- EMDB-24772: C17 reconstruction for Outer Membrane Core Complex (OMCC) of Type... -
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IDまたはキーワード:
読み込み中...
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基本情報
登録情報
データベース: EMDB / ID: EMD-24772
タイトル
C17 reconstruction for Outer Membrane Core Complex (OMCC) of Type IV Secretion System (T4SS) encoded by a plasmid overproducing TraV, TraK and TraB of pED208
マップデータ
C17 reconstruction for Outer Membrane Core Complex (OMCC) of Type IV Secretion System (T4SS) encoded by a plasmid overproducing TraV, TraK and TraB of pED208
試料
細胞器官・細胞要素: Outer membrane core complex of T4SS encoded by a plasmid overproducing TraV, TraK and TraB of pED208
機能・相同性
Type IV conjugative transfer system protein TraV / Type IV conjugative transfer system lipoprotein (TraV) / Bacterial conjugation TrbI-like protein / Type IV secretion system, VirB10 / TraB / TrbI / Prokaryotic membrane lipoprotein lipid attachment site profile. / TraV / TraB
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
米国
引用
ジャーナル: Nat Commun / 年: 2022 タイトル: Structure of a type IV secretion system core complex encoded by multi-drug resistance F plasmids. 著者: Xiangan Liu / Pratick Khara / Matthew L Baker / Peter J Christie / Bo Hu / 要旨: Bacterial type IV secretion systems (T4SSs) are largely responsible for the proliferation of multi-drug resistance. We solved the structure of the outer-membrane core complex (OMCC) of a T4SS encoded ...Bacterial type IV secretion systems (T4SSs) are largely responsible for the proliferation of multi-drug resistance. We solved the structure of the outer-membrane core complex (OMCC) of a T4SS encoded by a conjugative F plasmid at <3.0 Å resolution by cryoelectron microscopy. The OMCC consists of a 13-fold symmetrical outer ring complex (ORC) built from 26 copies of TraK and TraV C-terminal domains, and a 17-fold symmetrical central cone (CC) composed of 17 copies of TraB β-barrels. Domains of TraV and TraB also bind the CC and ORC substructures, establishing that these proteins undergo an intraprotein symmetry alteration to accommodate the C13:C17 symmetry mismatch. We present evidence that other pED208-encoded factors stabilize the C13:C17 architecture and define the importance of TraK, TraV and TraB domains to T4SS function. This work identifies OMCC structural motifs of proposed importance for structural transitions associated with F plasmid dissemination and F pilus biogenesis.
ダウンロード / ファイル: emd_24772.map.gz / 形式: CCP4 / 大きさ: 244.1 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
C17 reconstruction for Outer Membrane Core Complex (OMCC) of Type IV Secretion System (T4SS) encoded by a plasmid overproducing TraV, TraK and TraB of pED208
ボクセルのサイズ
X=Y=Z: 1.0652 Å
密度
表面レベル
登録者による: 0.38 / ムービー #1: 0.38
最小 - 最大
-0.9331222 - 1.9079931
平均 (標準偏差)
0.005173705 (±0.059405543)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
0
0
0
サイズ
400
400
400
Spacing
400
400
400
セル
A=B=C: 426.08 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
1.0652
1.0652
1.0652
M x/y/z
400
400
400
origin x/y/z
0.000
0.000
0.000
length x/y/z
426.080
426.080
426.080
α/β/γ
90.000
90.000
90.000
start NX/NY/NZ
116
110
112
NX/NY/NZ
123
123
149
MAP C/R/S
1
2
3
start NC/NR/NS
0
0
0
NC/NR/NS
400
400
400
D min/max/mean
-0.933
1.908
0.005
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添付データ
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試料の構成要素
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全体 : Outer membrane core complex of T4SS encoded by a plasmid overprod...
全体
名称: Outer membrane core complex of T4SS encoded by a plasmid overproducing TraV, TraK and TraB of pED208
要素
細胞器官・細胞要素: Outer membrane core complex of T4SS encoded by a plasmid overproducing TraV, TraK and TraB of pED208
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超分子 #1: Outer membrane core complex of T4SS encoded by a plasmid overprod...
超分子
名称: Outer membrane core complex of T4SS encoded by a plasmid overproducing TraV, TraK and TraB of pED208 タイプ: organelle_or_cellular_component / ID: 1 / 親要素: 0
由来(天然)
生物種: Escherichia coli BL21(DE3) (大腸菌)
組換発現
生物種: Escherichia coli BL21(DE3) (大腸菌)
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実験情報
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構造解析
手法
クライオ電子顕微鏡法
解析
単粒子再構成法
試料の集合状態
particle
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試料調製
緩衝液
pH: 8
凍結
凍結剤: ETHANE
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電子顕微鏡法
顕微鏡
FEI TITAN KRIOS
撮影
フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 平均電子線量: 40.0 e/Å2