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- EMDB-24652: Structure of ribosomal complex bound with Rbg1/Tma46 -

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Basic information

Entry
Database: EMDB / ID: EMD-24652
TitleStructure of ribosomal complex bound with Rbg1/Tma46
Map data
Sample
  • Complex: Ribosomal complex with Rbg1/Tma46
    • Complex: 60S subunitEukaryotic large ribosomal subunit (60S)
      • RNA: x 3 types
      • Protein or peptide: x 44 types
    • Complex: 40S subunit
      • RNA: x 1 types
      • Protein or peptide: x 33 types
    • Complex: Rbg1/Tma46
      • Protein or peptide: x 2 types
    • Complex: A-tRNA
      • RNA: x 1 types
    • Complex: P-tRNA
      • RNA: x 1 types
    • Complex: eIF5A
      • Protein or peptide: x 1 types
    • Complex: mRNAMessenger RNA
      • RNA: x 1 types
  • Ligand: x 3 types
Function / homology
Function and homology information


positive regulation of translational termination / positive regulation of translational elongation / ribosomal subunit / pre-mRNA 5'-splice site binding / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / Major pathway of rRNA processing in the nucleolus and cytosol / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit ...positive regulation of translational termination / positive regulation of translational elongation / ribosomal subunit / pre-mRNA 5'-splice site binding / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / Major pathway of rRNA processing in the nucleolus and cytosol / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Formation of a pool of free 40S subunits / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / negative regulation of mRNA splicing, via spliceosome / L13a-mediated translational silencing of Ceruloplasmin expression / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translation elongation factor activity / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / ribosomal large subunit biogenesis / translation initiation factor activity / cytosolic ribosome / macroautophagy / ribosomal small subunit assembly / rRNA processing / cytosolic small ribosomal subunit / ribosome binding / large ribosomal subunit / ribosome biogenesis / cytoplasmic translation / small ribosomal subunit / 5S rRNA binding / cytosolic large ribosomal subunit / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / GTP binding / RNA binding / zinc ion binding / metal ion binding / nucleus / cytosol / cytoplasm
Similarity search - Function
ZC3H15/TMA46 family, C-terminal / DRG Family Regulatory Proteins, Tma46 / GTP binding protein, second domain / C-terminal region of MMR_HSR1 domain / GTP1/OBG, conserved site / GTP1/OBG family signature. / Translation elongation factor, IF5A, hypusine site / Eukaryotic initiation factor 5A hypusine signature. / Eukaryotic elongation factor 5A hypusine, DNA-binding OB fold / Translation elongation factor IF5A-like ...ZC3H15/TMA46 family, C-terminal / DRG Family Regulatory Proteins, Tma46 / GTP binding protein, second domain / C-terminal region of MMR_HSR1 domain / GTP1/OBG, conserved site / GTP1/OBG family signature. / Translation elongation factor, IF5A, hypusine site / Eukaryotic initiation factor 5A hypusine signature. / Eukaryotic elongation factor 5A hypusine, DNA-binding OB fold / Translation elongation factor IF5A-like / Translation elongation factor, IF5A C-terminal / Eukaryotic elongation factor 5A hypusine, DNA-binding OB fold / TGS domain / Zinc finger, CCCH-type superfamily / zinc finger / 60S acidic ribosomal protein P0 / Zinc finger, CCCH-type / Zinc finger C3H1-type profile. / TGS domain profile. / TGS / TGS-like / OBG-type guanine nucleotide-binding (G) domain / OBG-type guanine nucleotide-binding (G) domain profile. / 50S ribosome-binding GTPase / GTP binding domain / : / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S12e / 50S ribosomal protein L10, insertion domain superfamily / : / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / Ribosomal protein S21e, conserved site / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S19e, conserved site / S27a-like superfamily / Ribosomal protein S26e / Ribosomal protein L29e / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S25 / Ribosomal protein S26e signature. / Ribosomal protein S17e, conserved site / Ribosomal protein S2, eukaryotic / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal L29e protein family / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein S8e subdomain, eukaryotes / S25 ribosomal protein / Ribosomal protein L10e, conserved site / Ribosomal protein L10e / Ribosomal protein L27e, conserved site / Ribosomal protein S19A/S15e / Ribosomal protein S21e signature. / Ribosomal protein S30 / Ribosomal protein S3Ae, conserved site / Ribosomal protein S12e signature. / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal protein S27a / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S19e / Ribosomal_S19e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S8e, conserved site / 40S ribosomal protein S11, N-terminal / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein S6, eukaryotic / Ribosomal protein L24e, conserved site / Ribosomal protein S7e / 40S ribosomal protein S4, C-terminal domain / Ribosomal protein L34e, conserved site / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal S17 / Ribosomal protein S19e signature. / Ribosomal protein L44e / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal protein S19e / Ribosomal protein S27, zinc-binding domain superfamily / Ribosomal L38e protein family / Ribosomal L22e protein family / Ribosomal protein L23/L25, N-terminal / 40S Ribosomal protein S10 / Ribosomal protein S17, archaeal/eukaryotic / Ribosomal protein S27 / Ribosomal protein S28e conserved site / Ribosomal protein S6/S6e/A/B/2, conserved site
Similarity search - Domain/homology
RPS5 isoform 1 / Small ribosomal subunit protein eS1 / RPS22A isoform 1 / 60S acidic ribosomal protein P0 / RPS31 isoform 1 / RPL38 isoform 1 / RPL10 isoform 1 / RPS29A isoform 1 / RPS20 isoform 1 / RPS2 isoform 1 ...RPS5 isoform 1 / Small ribosomal subunit protein eS1 / RPS22A isoform 1 / 60S acidic ribosomal protein P0 / RPS31 isoform 1 / RPL38 isoform 1 / RPL10 isoform 1 / RPS29A isoform 1 / RPS20 isoform 1 / RPS2 isoform 1 / 60S ribosomal protein L29 / BJ4_G0051200.mRNA.1.CDS.1 / Eukaryotic translation initiation factor 5A / RPL24A isoform 1 / RPL11B isoform 1 / 40S ribosomal protein S25 / 40S ribosomal protein S26 / RPL5 isoform 1 / 40S ribosomal protein S8 / RPL32 isoform 1 / RPL12A isoform 1 / 40S ribosomal protein S3 / RPL4A isoform 1 / Large ribosomal subunit protein uL3 / RPS15 isoform 1 / RPS28A isoform 1 / BJ4_G0035820.mRNA.1.CDS.1 / 40S ribosomal protein S12 / 60S ribosomal protein L2-B / 40S ribosomal protein S13 / 60S ribosomal protein L31-A / 40S ribosomal protein S11-B / EM14S01-3B_G0023620.mRNA.1.CDS.1 / 60S ribosomal protein L25 / BJ4_G0018510.mRNA.1.CDS.1 / 40S ribosomal protein S4 / 40S ribosomal protein S14-B / 60S ribosomal protein L23-B / 60S ribosomal protein L16-A / 60S ribosomal protein L28 / 60S ribosomal protein L34-A / 40S ribosomal protein S10-A / 60S ribosomal protein L27 / 40S ribosomal protein S0 / 60S ribosomal protein L22-A / 40S ribosomal protein S30 / 60S ribosomal protein L18-B / 40S ribosomal protein S7 / 60S ribosomal protein L13 / 40S ribosomal protein S18-B / 40S ribosomal protein S6 / 60S ribosomal protein L39 / 40S ribosomal protein S24 / 40S ribosomal protein S23 / 60S ribosomal protein L42-B / 60S ribosomal protein L17-A / Ribosomal protein L37 / 60S ribosomal protein L6 / EM14S01-3B_G0023350.mRNA.1.CDS.1 / EM14S01-3B_G0022060.mRNA.1.CDS.1 / 40S ribosomal protein S19-A / EM14S01-3B_G0017920.mRNA.1.CDS.1 / 60S ribosomal protein L7-A / Ubiquitin / EM14S01-3B_G0041280.mRNA.1.CDS.1 / EM14S01-3B_G0018500.mRNA.1.CDS.1 / Large ribosomal subunit protein uL6A / Large ribosomal subunit protein uL24A / Large ribosomal subunit protein eL36A / Large ribosomal subunit protein eL15A / Small ribosomal subunit protein eS21A / Large ribosomal subunit protein eL20A / Large ribosomal subunit protein eL43A / Large ribosomal subunit protein eL19A / Small ribosomal subunit protein eS32A / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein eL8A / Small ribosomal subunit protein eS27A / Large ribosomal subunit protein eL14A
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (brewer's yeast) / Baker's yeast (brewer's yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.23 Å
AuthorsZeng F / Li X / Pires-Alves M / Chen X / Hawk CW / Jin H
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01-GM120552 United States
CitationJournal: Cell Rep / Year: 2021
Title: Conserved heterodimeric GTPase Rbg1/Tma46 promotes efficient translation in eukaryotic cells.
Authors: Fuxing Zeng / Xin Li / Melissa Pires-Alves / Xin Chen / Christopher W Hawk / Hong Jin /
Abstract: Conserved developmentally regulated guanosine triphosphate (GTP)-binding proteins (Drgs) and their binding partner Drg family regulatory proteins (Dfrps) are important for embryonic development, ...Conserved developmentally regulated guanosine triphosphate (GTP)-binding proteins (Drgs) and their binding partner Drg family regulatory proteins (Dfrps) are important for embryonic development, cellular growth control, differentiation, and proliferation. Here, we report that the yeast Drg1/Dfrp1 ortholog Rbg1/Tma46 facilitates translational initiation, elongation, and termination by suppressing prolonged ribosome pausing. Consistent with the genome-wide observations, deletion of Rbg1 exacerbates the growth defect resulting from translation stalling, and Rbg1 stabilizes mRNAs against no-go decay. Furthermore, we provide a cryoelectron microscopy (cryo-EM) structure of the 80S ribosome bound with Rbg1/Tma46 that reveals the molecular interactions responsible for Rbg1/Tma46 function. The Rbg1 subunit binds to the GTPase association center of the ribosome and the A-tRNA, and the N-terminal zinc finger domain of the Tma46 subunit binds to the 40S, establishing an interaction critical for the ribosomal association. Our results answer the fundamental question of how a paused ribosome resumes translation and show that Drg1/Dfrp1 play a critical role in ensuring orderly translation.
History
DepositionAug 9, 2021-
Header (metadata) releaseNov 10, 2021-
Map releaseNov 10, 2021-
UpdateNov 10, 2021-
Current statusNov 10, 2021Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.003
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.003
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7rr5
  • Surface level: 0.003
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_24652.png

Downloads & links

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Map

FileDownload / File: emd_24652.map.gz / Format: CCP4 / Size: 343 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.08 Å
Density
Contour LevelBy AUTHOR: 0.003 / Movie #1: 0.003
Minimum - Maximum-0.029108446 - 0.047066502
Average (Standard dev.)3.5638895e-05 (±0.0020829395)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions448448448
Spacing448448448
CellA=B=C: 483.84003 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.081.081.08
M x/y/z448448448
origin x/y/z0.0000.0000.000
length x/y/z483.840483.840483.840
α/β/γ90.00090.00090.000
start NX/NY/NZ45440
NX/NY/NZ103105148
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS448448448
D min/max/mean-0.0290.0470.000

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Supplemental data

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Sample components

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Entire : Ribosomal complex with Rbg1/Tma46

EntireName: Ribosomal complex with Rbg1/Tma46
Components
  • Complex: Ribosomal complex with Rbg1/Tma46
    • Complex: 60S subunitEukaryotic large ribosomal subunit (60S)
      • RNA: 25S rRNA
      • RNA: 5S rRNA5S ribosomal RNA
      • RNA: 5.8S rRNA5.8S ribosomal RNA
      • Protein or peptide: 60S ribosomal protein L2-BRibosome
      • Protein or peptide: RPL3 isoform 1
      • Protein or peptide: RPL4A isoform 1
      • Protein or peptide: RPL5 isoform 1
      • Protein or peptide: 60S ribosomal protein L6
      • Protein or peptide: 60S ribosomal protein L7-ARibosome
      • Protein or peptide: 60S ribosomal protein L8-ARibosome
      • Protein or peptide: 60S ribosomal protein L9-ARibosome
      • Protein or peptide: RPL10 isoform 1
      • Protein or peptide: RPL11B isoform 1
      • Protein or peptide: 60S ribosomal protein L13
      • Protein or peptide: 60S ribosomal protein L14-ARibosome
      • Protein or peptide: 60S ribosomal protein L15-ARibosome
      • Protein or peptide: 60S ribosomal protein L16-ARibosome
      • Protein or peptide: 60S ribosomal protein L17-ARibosome
      • Protein or peptide: 60S ribosomal protein L18-BRibosome
      • Protein or peptide: 60S ribosomal protein L19-ARibosome
      • Protein or peptide: 60S ribosomal protein L20-ARibosome
      • Protein or peptide: 60S ribosomal protein L21-ARibosome
      • Protein or peptide: 60S ribosomal protein L22-ARibosome
      • Protein or peptide: 60S ribosomal protein L23-BRibosome
      • Protein or peptide: RPL24A isoform 1
      • Protein or peptide: 60S ribosomal protein L25
      • Protein or peptide: 60S ribosomal protein L26-ARibosome
      • Protein or peptide: 60S ribosomal protein L27
      • Protein or peptide: 60S ribosomal protein L28
      • Protein or peptide: RPL29 isoform 1
      • Protein or peptide: 60S ribosomal protein L30
      • Protein or peptide: 60S ribosomal protein L31-ARibosome
      • Protein or peptide: RPL32 isoform 1
      • Protein or peptide: 60S ribosomal protein L33-ARibosome
      • Protein or peptide: 60S ribosomal protein L34-ARibosome
      • Protein or peptide: 60S ribosomal protein L35-ARibosome
      • Protein or peptide: 60S ribosomal protein L36-ARibosome
      • Protein or peptide: Ribosomal protein L37
      • Protein or peptide: RPL38 isoform 1
      • Protein or peptide: 60S ribosomal protein L39
      • Protein or peptide: Ubiquitin-60S ribosomal protein L40
      • Protein or peptide: 60S ribosomal protein L41-ARibosome
      • Protein or peptide: 60S ribosomal protein L42-ARibosome
      • Protein or peptide: 60S ribosomal protein L43-ARibosome
      • Protein or peptide: RPP0 isoform 1
      • Protein or peptide: RPL12A isoform 1
      • Protein or peptide: 60S ribosomal protein L1
    • Complex: 40S subunit
      • RNA: 18S rRNA18S ribosomal RNA
      • Protein or peptide: 40S ribosomal protein S0
      • Protein or peptide: RPS1A isoform 1
      • Protein or peptide: RPS2 isoform 1
      • Protein or peptide: RPS3 isoform 1
      • Protein or peptide: 40S ribosomal protein S4
      • Protein or peptide: Rps5p
      • Protein or peptide: 40S ribosomal protein S6
      • Protein or peptide: 40S ribosomal protein S7
      • Protein or peptide: RPS8A isoform 1
      • Protein or peptide: 40S ribosomal protein S9-ARibosome
      • Protein or peptide: 40S ribosomal protein S10-ARibosome
      • Protein or peptide: 40S ribosomal protein S11-BRibosome
      • Protein or peptide: 40S ribosomal protein S12
      • Protein or peptide: 40S ribosomal protein S13
      • Protein or peptide: 40S ribosomal protein S14-BRibosome
      • Protein or peptide: RPS15 isoform 1
      • Protein or peptide: 40S ribosomal protein S16-ARibosome
      • Protein or peptide: 40S ribosomal protein S17-ARibosome
      • Protein or peptide: 40S ribosomal protein S18-BRibosome
      • Protein or peptide: 40S ribosomal protein S19-ARibosome
      • Protein or peptide: RPS20 isoform 1
      • Protein or peptide: 40S ribosomal protein S21-ARibosome
      • Protein or peptide: RPS22A isoform 1
      • Protein or peptide: 40S ribosomal protein S23
      • Protein or peptide: 40S ribosomal protein S24
      • Protein or peptide: RPS25A isoform 1
      • Protein or peptide: RPS26B isoform 1
      • Protein or peptide: 40S ribosomal protein S27-ARibosome
      • Protein or peptide: RPS28A isoform 1
      • Protein or peptide: RPS29A isoform 1
      • Protein or peptide: 40S ribosomal protein S30
      • Protein or peptide: Ubiquitin-40S ribosomal protein S31
      • Protein or peptide: Guanine nucleotide-binding protein subunit beta-like protein
    • Complex: Rbg1/Tma46
      • Protein or peptide: Ribosome-interacting GTPase 1
      • Protein or peptide: Translation machinery-associated protein 46
    • Complex: A-tRNA
      • RNA: A-tRNA
    • Complex: P-tRNA
      • RNA: P-tRNA
    • Complex: eIF5A
      • Protein or peptide: Eukaryotic translation initiation factor 5A
    • Complex: mRNAMessenger RNA
      • RNA: mRNAMessenger RNA
  • Ligand: MAGNESIUM ION
  • Ligand: ASPARTIC ACID
  • Ligand: ZINC ION

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Supramolecule #1: Ribosomal complex with Rbg1/Tma46

SupramoleculeName: Ribosomal complex with Rbg1/Tma46 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#87
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)

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Supramolecule #2: 60S subunit

SupramoleculeName: 60S subunit / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#46, #87
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)

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Supramolecule #3: 40S subunit

SupramoleculeName: 40S subunit / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #47-#80
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)

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Supramolecule #4: Rbg1/Tma46

SupramoleculeName: Rbg1/Tma46 / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #85-#86
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)

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Supramolecule #5: A-tRNA

SupramoleculeName: A-tRNA / type: complex / ID: 5 / Parent: 1 / Macromolecule list: #81
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)

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Supramolecule #6: P-tRNA

SupramoleculeName: P-tRNA / type: complex / ID: 6 / Parent: 1 / Macromolecule list: #82
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)

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Supramolecule #7: eIF5A

SupramoleculeName: eIF5A / type: complex / ID: 7 / Parent: 1 / Macromolecule list: #84
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)

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Supramolecule #8: mRNA

SupramoleculeName: mRNA / type: complex / ID: 8 / Parent: 1 / Macromolecule list: #83
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)

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Macromolecule #1: 25S rRNA

MacromoleculeName: 25S rRNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 1.0974938749999998 MDa
SequenceString: GUUUGACCUC AAAUCAGGUA GGAGUACCCG CUGAACUUAA GCAUAUCAAU AAGCGGAGGA AAAGAAACCA ACCGGGAUUG CCUUAGUAA CGGCGAGUGA AGCGGCAAAA GCUCAAAUUU GAAAUCUGGU ACCUUCGGUG CCCGAGUUGU AAUUUGGAGA G GGCAACUU ...String:
GUUUGACCUC AAAUCAGGUA GGAGUACCCG CUGAACUUAA GCAUAUCAAU AAGCGGAGGA AAAGAAACCA ACCGGGAUUG CCUUAGUAA CGGCGAGUGA AGCGGCAAAA GCUCAAAUUU GAAAUCUGGU ACCUUCGGUG CCCGAGUUGU AAUUUGGAGA G GGCAACUU UGGGGCCGUU CCUUGUCUAU GUUCCUUGGA ACAGGACGUC AUAGAGGGUG AGAAUCCCGU GUGGCGAGGA GU GCGGUUC UUUGUAAAGU GCCUUCGAAG AGUCGAGUUG UUUGGGAAUG CAGCUCUAAG UGGGUGGUAA AUUCCAUCUA AAG CUAAAU AUUGGCGAGA GACCGAUAGC GAACAAGUAC AGUGAUGGAA AGAUGAAAAG AACUUUGAAA AGAGAGUGAA AAAG UACGU GAAAUUGUUG AAAGGGAAGG GCAUUUGAUC AGACAUGGUG UUUUGUGCCC UCUGCUCCUU GUGGGUAGGG GAAUC UCGC AUUUCACUGG GCCAGCAUCA GUUUUGGUGG CAGGAUAAAU CCAUAGGAAU GUAGCUUGCC UCGGUAAGUA UUAUAG CCU GUGGGAAUAC UGCCAGCUGG GACUGAGGAC UGCGACGUAA GUCAAGGAUG CUGGCAUAAU GGUUAUAUGC CGCCCGU CU UGAAACACGG ACCAAGGAGU CUAACGUCUA UGCGAGUGUU UGGGUGUAAA ACCCAUACGC GUAAUGAAAG UGAACGUA G GUUGGGGCCU CGCAAGAGGU GCACAAUCGA CCGAUCCUGA UGUCUUCGGA UGGAUUUGAG UAAGAGCAUA GCUGUUGGG ACCCGAAAGA UGGUGAACUA UGCCUGAAUA GGGUGAAGCC AGAGGAAACU CUGGUGGAGG CUCGUAGCGG UUCUGACGUG CAAAUCGAU CGUCGAAUUU GGGUAUAGGG GCGAAAGACU AAUCGAACCA UCUAGUAGCU GGUUCCUGCC GAAGUUUCCC U CAGGAUAG CAGAAGCUCG UAUCAGUUUU AUGAGGUAAA GCGAAUGAUU AGAGGUUCCG GGGUCGAAAU GACCUUGACC UA UUCUCAA ACUUUAAAUA UGUAAGAAGU CCUUGUUACU UAAUUGAACG UGGACAUUUG AAUGAAGAGC UUUUAGUGGG CCA UUUUUG GUAAGCAGAA CUGGCGAUGC GGGAUGAACC GAACGUAGAG UUAAGGUGCC GGAAUACACG CUCAUCAGAC ACCA CAAAA GGUGUUAGUU CAUCUAGACA GCCGGACGGU GGCCAUGGAA GUCGGAAUCC GCUAAGGAGU GUGUAACAAC UCACC GGCC GAAUGAACUA GCCCUGAAAA UGGAUGGCGC UCAAGCGUGU UACCUAUACU CUACCGUCAG GGUUGAUAUG AUGCCC UGA CGAGUAGGCA GGCGUGGAGG UCAGUGACGA AGCCUAGACC GUAAGGUCGG GUCGAACGGC CUCUAGUGCA GAUCUUG GU GGUAGUAGCA AAUAUUCAAA UGAGAACUUU GAAGACUGAA GUGGGGAAAG GUUCCACGUC AACAGCAGUU GGACGUGG G UUAGUCGAUC CUAAGAGAUG GGGAAGCUCC GUUUCAAAGG CCUGAUUUUA UGCAGGCCAC CAUCGAAAGG GAAUCCGGU UAAGAUUCCG GAACCUGGAU AUGGAUUCUU CACGGUAACG UAACUGAAUG UGGAGACGUC GGCGCGAGCC CUGGGAGGAG UUAUCUUUU CUUCUUAACA GCUUAUCACC CCGGAAUUGG UUUAUCCGGA GAUGGGGUCU UAUGGCUGGA AGAGGCCAGC A CCUUUGCU GGCUCCGGUG CGCUUGUGAC GGCCCGUGAA AAUCCACAGG AAGGAAUAGU UUUCAUGCCA GGUCGUACUG AU AACCGCA GCAGGUCUCC AAGGUGAACA GCCUCUAGUU GAUAGAAUAA UGUAGAUAAG GGAAGUCGGC AAAAUAGAUC CGU AACUUC GGGAUAAGGA UUGGCUCUAA GGGUCGGGUA GUGAGGGCCU UGGUCAGACG CAGCGGGCGU GCUUGUGGAC UGCU UGGUG GGGCUUGCUC UGCUAGGCGG ACUACUUGCG UGCCUUGUUG UAGACGGCCU UGGUAGGUCU CUUGUAGACC GUCGC UUGC UACAAUUAAC GAUCAACUUA GAACUGGUAC GGACAAGGGG AAUCUGACUG UCUAAUUAAA ACAUAGCAUU GCGAUG GUC AGAAAGUGAU GUUGACGCAA UGUGAUUUCU GCCCAGUGCU CUGAAUGUCA AAGUGAAGAA AUUCAACCAA GCGCGGG UA AACGGCGGGA GUAACUAUGA CUCUCUUAAG GUAGCCAAAU GCCUCGUCAU CUAAUUAGUG ACGCGCAUGA AUGGAUUA A CGAGAUUCCC ACUGUCCCUA UCUACUAUCU AGCGAAACCA CAGCCAAGGG AACGGGCUUG GCAGAAUCAG CGGGGAAAG AAGACCCUGU UGAGCUUGAC UCUAGUUUGA CAUUGUGAAG AGACAUAGAG GGUGUAGAAU AAGUGGGAGC UUCGGCGCCA GUGAAAUAC CACUACCUUU AUAGUUUCUU UACUUAUUCA AUGAAGCGGA GCUGGAAUUC AUUUUCCACG UUCUAGCAUU C AAGGUCCC AUUCGGGGCU GAUCCGGGUU GAAGACAUUG UCAGGUGGGG AGUUUGGCUG GGGCGGCACA UCUGUUAAAC GA UAACGCA GAUGUCCUAA GGGGGGCUCA UGGAGAACAG AAAUCUCCAG UAGAACAAAA GGGUAAAAGC CCCCUUGAUU UUG AUUUUC AGUGUGAAUA CAAACCAUGA AAGUGUGGCC UAUCGAUCCU UUAGUCCCUC GGAAUUUGAG GCUAGAGGUG CCAG AAAAG UUACCACAGG GAUAACUGGC UUGUGGCAGU CAAGCGUUCA UAGCGACAUU GCUUUUUGAU UCUUCGAUGU CGGCU CUUC CUAUCAUACC GAAGCAGAAU UCGGUAAGCG UUGGAUUGUU CACCCACUAA UAGGGAACGU GAGCUGGGUU UAGACC GUC GUGAGACAGG UUAGUUUUAC CCUACUGAUG AAUGUUACCG CAAUAGUAAU UGAACUUAGU ACGAGAGGAA CAGUUCA UU CGGAUAAUUG GUUUUUGCGG CUGUCUGAUC AGGCAUUGCC GCGAAGCUAC CAUCCGCUGG AUUAUGGCUG AACGCCUC U AAGUCAGAAU CCAUGCUAGA ACGCGGUGAU UUCUUUGCUC CACACAAUAU AGAUGGAUAC GAAUAAGGCG UCCUUGUGG CGUCGCUGAA CCAUAGCAGG CUAGCAACGG UGCACUUGGC GGAAAGGCCU UGGGUGCUUG CUGGCGAAUU GCAAUGUCAU UUUGCGUGG GGAUAAAUCA UUUGUAUACG ACUUAGAUGU ACAACGGGGU AUUGUAAGCA GUAGAGUAGC CUUGUUGUUA C GAUCUGCU GAGAUUAAGC CUUUGUUGUC UGAUUUGU

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Macromolecule #2: 5S rRNA

MacromoleculeName: 5S rRNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 38.951105 KDa
SequenceString:
GGUUGCGGCC AUAUCUACCA GAAAGCACCG UUUCCCGUCC GAUCAACUGU AGUUAAGCUG GUAAGAGCCU GACCGAGUAG UGUAGUGGG UGACCAUACG CGAAACUCAG GUGCUGCAAU CU

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Macromolecule #3: 5.8S rRNA

MacromoleculeName: 5.8S rRNA / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 50.682922 KDa
SequenceString:
AAACUUUCAA CAACGGAUCU CUUGGUUCUC GCAUCGAUGA AGAACGCAGC GAAAUGCGAU ACGUAAUGUG AAUUGCAGAA UUCCGUGAA UCAUCGAAUC UUUGAACGCA CAUUGCGCCC CUUGGUAUUC CAGGGGGCAU GCCUGUUUGA GCGUCAUUU

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Macromolecule #47: 18S rRNA

MacromoleculeName: 18S rRNA / type: rna / ID: 47 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 579.761938 KDa
SequenceString: UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUG CGAAUGGCUC AUUAAAUCAG UUAUCGUUUA UUUGAUAGUU CCUUUACUAC AUGGUAUAAC UGUGGUAAUU C UAGAGCUA ...String:
UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUG CGAAUGGCUC AUUAAAUCAG UUAUCGUUUA UUUGAUAGUU CCUUUACUAC AUGGUAUAAC UGUGGUAAUU C UAGAGCUA AUACAUGCUU AAAAUCUCGA CCCUUUGGAA GAGAUGUAUU UAUUAGAUAA AAAAUCAAUG UCUUCGGACU CU UUGAUGA UUCAUAAUAA CUUUUCGAAU CGCAUGGCCU UGUGCUGGCG AUGGUUCAUU CAAAUUUCUG CCCUAUCAAC UUU CGAUGG UAGGAUAGUG GCCUACCAUG GUUUCAACGG GUAACGGGGA AUAAGGGUUC GAUUCCGGAG AGGGAGCCUG AGAA ACGGC UACCACAUCC AAGGAAGGCA GCAGGCGCGC AAAUUACCCA AUCCUAAUUC AGGGAGGUAG UGACAAUAAA UAACG AUAC AGGGCCCAUU CGGGUCUUGU AAUUGGAAUG AGUACAAUGU AAAUACCUUA ACGAGGAACA AUUGGAGGGC AAGUCU GGU GCCAGCAGCC GCGGUAAUUC CAGCUCCAAU AGCGUAUAUU AAAGUUGUUG CAGUUAAAAA GCUCGUAGUU GAACUUU GG GCCCGGUUGG CCGGUCCGAU UUUUUCGUGU ACUGGAUUUC CAACGGGGCC UUUCCUUCUG GCUAACCUUG AGUCCUUG U GGCUCUUGGC GAACCAGGAC UUUUACUUUG AAAAAAUUAG AGUGUUCAAA GCAGGCGUAU UGCUCGAAUA UAUUAGCAU GGAAUAAUAG AAUAGGACGU UUGGUUCUAU UUUGUUGGUU UCUAGGACCA UCGUAAUGAU UAAUAGGGAC GGUCGGGGGC AUCAGUAUU CAAUUGUCAG AGGUGAAAUU CUUGGAUUUA UUGAAGACUA ACUACUGCGA AAGCAUUUGC CAAGGACGUU U UCAUUAAU CAAGAACGAA AGUUAGGGGA UCGAAGAUGA UCAGAUACCG UCGUAGUCUU AACCAUAAAC UAUGCCGACU AG GGAUCGG GUGGUGUUUU UUUAAUGACC CACUCGGCAC CUUACGAGAA AUCAAAGUCU UUGGGUUCUG GGGGGAGUAU GGU CGCAAG GCUGAAACUU AAAGGAAUUG ACGGAAGGGC ACCACCAGGA GUGGAGCCUG CGGCUUAAUU UGACUCAACA CGGG GAAAC UCACCAGGUC CAGACACAAU AAGGAUUGAC AGAUUGAGAG CUCUUUCUUG AUUUUGUGGG UGGUGGUGCA UGGCC GUUC UUAGUUGGUG GAGUGAUUUG UCUGCUUAAU UGCGAUAACG AACGAGACCU UAACCUACUA AAUAGUGGUG CUAGCA UUU GCUGGUUAUC CACUUCUUAG AGGGACUAUC GGUUUCAAGC CGAUGGAAGU UUGAGGCAAU AACAGGUCUG UGAUGCC CU UAGACGUUCU GGGCCGCACG CGCGCUACAC UGACGGAGCC AGCGAGUCUA ACCUUGGCCG AGAGGUCUUG GUAAUCUU G UGAAACUCCG UCGUGCUGGG GAUAGAGCAU UGUAAUUAUU GCUCUUCAAC GAGGAAUUCC UAGUAAGCGC AAGUCAUCA GCUUGCGUUG AUUACGUCCC UGCCCUUUGU ACACACCGCC CGUCGCUAGU ACCGAUUGAA UGGCUUAGUG AGGCCUCAGG AUCUGCUUA GAGAAGGGGG CAACUCCAUC UCAGAGCGGA GAAUUUGGAC AAACUUGGUC AUUUAGAGGA ACUAAAAGUC G UAACAAGG UUUCCGUAGG UGAACCUGCG GAAGGAUCAU UA

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Macromolecule #81: A-tRNA

MacromoleculeName: A-tRNA / type: rna / ID: 81 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 24.355369 KDa
SequenceString:
GGGUCGUUAG CUCAGUUGGU AGAGCAGUUG ACUUUUUAUC AAUUGGUCGC AGGUUCGAAU CCUGCACGAC CCACCA

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Macromolecule #82: P-tRNA

MacromoleculeName: P-tRNA / type: rna / ID: 82
Details: Althrough the position 34 is G or A in NCBI database. But it can only be U base on the density. It might be a varient tRNA from the yeast.
Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 24.445512 KDa
SequenceString:
UUCCUCGUGG CCCAAUGGUC ACGGCGUCUG GCUUCGAACC AGAAGAUUCC AGGUUCAAGU CCUGGCGGGG AAGCCA

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Macromolecule #83: mRNA

MacromoleculeName: mRNA / type: rna / ID: 83 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 5.232232 KDa
SequenceString:
UGGAUGGAAC GAAAAA

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Macromolecule #4: 60S ribosomal protein L2-B

MacromoleculeName: 60S ribosomal protein L2-B / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 27.463574 KDa
SequenceString: MGRVIRNQRK GAGSIFTSHT RLRQGAAKLR TLDYAERHGY IRGIVKQIVH DSGRGAPLAK VVFRDPYKYR LREEIFIANE GVHTGQFIY AGKKASLNVG NVLPLGSVPE GTIVSNVEEK PGDRGALARA SGNYVIIIGH NPDENKTRVR LPSGAKKVIS S DARGVIGV ...String:
MGRVIRNQRK GAGSIFTSHT RLRQGAAKLR TLDYAERHGY IRGIVKQIVH DSGRGAPLAK VVFRDPYKYR LREEIFIANE GVHTGQFIY AGKKASLNVG NVLPLGSVPE GTIVSNVEEK PGDRGALARA SGNYVIIIGH NPDENKTRVR LPSGAKKVIS S DARGVIGV IAGGGRVDKP LLKAGRAFHK YRLKRNSWPK TRGVAMNPVD HPHGGGNHQH IGKASTISRG AVSGQKAGLI AA RRTGLLR GSQKTQD

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Macromolecule #5: RPL3 isoform 1

MacromoleculeName: RPL3 isoform 1 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 43.850793 KDa
SequenceString: MSHRKYEAPR HGHLGFLPRK RAASIRARVK AFPKDDRSKP VALTSFLGYK AGMTTIVRDL DRPGSKFHKR EVVEAVTVVD TPPVVVVGV VGYVETPRGL RSLTTVWAEH LSDEVKRRFY KNWYKSKKKA FTKYSAKYAQ DGAGIERELA RIKKYASVVR V LVHTQIRK ...String:
MSHRKYEAPR HGHLGFLPRK RAASIRARVK AFPKDDRSKP VALTSFLGYK AGMTTIVRDL DRPGSKFHKR EVVEAVTVVD TPPVVVVGV VGYVETPRGL RSLTTVWAEH LSDEVKRRFY KNWYKSKKKA FTKYSAKYAQ DGAGIERELA RIKKYASVVR V LVHTQIRK TPLAQKKAHL AEIQLNGGSI SEKVDWAREH FEKTVAVDSV FEQNEMIDAI AVTKGHGFEG VTHRWGTKKL PR KTHRGLR KVACIGAWHP AHVMWSVARA GQRGYHSRTS INHKIYRVGK GDDEANGATS FDRTKKTITP MGGFVHYGEI KND FIMVKG CIPGNRKRIV TLRKSLYTNT SRKALEEVSL KWIDTASKFG KGRFQTPAEK HAFMGTLKKD L

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Macromolecule #6: RPL4A isoform 1

MacromoleculeName: RPL4A isoform 1 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 39.159125 KDa
SequenceString: MSRPQVTVHS LTGEATANAL PLPAVFSAPI RPDIVHTVFT SVNKNKRQAY AVSEKAGHQT SAESWGTGRA VARIPRVGGG GTGRSGQGA FGNMCRGGRM FAPTKTWRKW NVKVNHNEKR YATASAIAAT AVASLVLARG HRVEKIPEIP LVVSTDLESI Q KTKEAVAA ...String:
MSRPQVTVHS LTGEATANAL PLPAVFSAPI RPDIVHTVFT SVNKNKRQAY AVSEKAGHQT SAESWGTGRA VARIPRVGGG GTGRSGQGA FGNMCRGGRM FAPTKTWRKW NVKVNHNEKR YATASAIAAT AVASLVLARG HRVEKIPEIP LVVSTDLESI Q KTKEAVAA LKAVGAHSDL LKVLKSKKLR AGKGKYRNRR WTQRRGPLVV YAEDNGIVKA LRNVPGVETA NVASLNLLQL AP GAHLGRF VIWTEAAFTK LDQVWGSETV ASSKVGYTLP SHIISTSDVT RIINSSEIQS AIRPAGQATQ KRTHVLKKNP LKN KQVLLR LNPYAKVFAA EKLGSKKAEK TGTKPAAVFT ETLKHD

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Macromolecule #7: RPL5 isoform 1

MacromoleculeName: RPL5 isoform 1 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 33.764828 KDa
SequenceString: MAFQKDAKSS AYSSRFQTPF RRRREGKTDY YQRKRLVTQH KAKYNTPKYR LVVRFTNKDI ICQIISSTIT GDVVLAAAYS HELPRYGIT HGLTNWAAAY ATGLLIARRT LQKLGLDETY KGVEEVEGEY ELTEAVEDGP RPFKVFLDIG LQRTTTGARV F GALKGASD ...String:
MAFQKDAKSS AYSSRFQTPF RRRREGKTDY YQRKRLVTQH KAKYNTPKYR LVVRFTNKDI ICQIISSTIT GDVVLAAAYS HELPRYGIT HGLTNWAAAY ATGLLIARRT LQKLGLDETY KGVEEVEGEY ELTEAVEDGP RPFKVFLDIG LQRTTTGARV F GALKGASD GGLYVPHSEN RFPGWDFETE EIDPELLRSY IFGGHVSQYM EELADDDEER FSELFKGYLA DDIDADSLED IY TSAHEAI RADPAFKPTE KKFTKEQYAA ESKKYRQTKL SKEERAARVA AKIAALAGQQ

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Macromolecule #8: 60S ribosomal protein L6

MacromoleculeName: 60S ribosomal protein L6 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 20.024541 KDa
SequenceString:
MTAQQAPKWY PSEDVAAPKK TRKAVRPQKL RASLVPGTVL ILLAGRFRGK RVVYLKHLED NTLLVTGPFK VNGVPLRRVN ARYVIATST KVSVEGVNVE KFNVEYFAKE KLTKKEKKEA NLFPEQQTKE IKTERVEDQK VVDKALLAEI KKTPLLKQYL S ASFSLKNG DKPHLLKF

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Macromolecule #9: 60S ribosomal protein L7-A

MacromoleculeName: 60S ribosomal protein L7-A / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 27.686281 KDa
SequenceString: MAAEKILTPE SQLKKSKAQQ KTAEQVAAER AARKAANKEK RAIILERNAA YQKEYETAER NIIQAKRDAK AAGSYYVEAQ HKLVFVVRI KGINKIPPKP RKVLQLLRLT RINSGTFVKV TKATLELLKL IEPYVAYGYP SYSTIRQLVY KRGFGKINKQ R VPLSDNAI ...String:
MAAEKILTPE SQLKKSKAQQ KTAEQVAAER AARKAANKEK RAIILERNAA YQKEYETAER NIIQAKRDAK AAGSYYVEAQ HKLVFVVRI KGINKIPPKP RKVLQLLRLT RINSGTFVKV TKATLELLKL IEPYVAYGYP SYSTIRQLVY KRGFGKINKQ R VPLSDNAI IEANLGKYGI LSIDDLIHEI ITVGPHFKQA NNFLWPFKLS NPSGGWGVPR KFKHFIQGGS FGNREEFINK LV KSMN

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Macromolecule #10: 60S ribosomal protein L8-A

MacromoleculeName: 60S ribosomal protein L8-A / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 28.17582 KDa
SequenceString: MAPGKKVAPA PFGAKSTKSN KTRNPLTHST PKNFGIGQAV QPKRNLSRYV KWPEYVRVQR QKKILSIRLK VPPTIAQFQY TLDRNTAAE TFKLFNKYRP ETAAEKKERL TKEAAAVAEG KSKQDASPKP YAVKYGLNHV VALIENKKAK LVLIANDVDP I ELVVFLPA ...String:
MAPGKKVAPA PFGAKSTKSN KTRNPLTHST PKNFGIGQAV QPKRNLSRYV KWPEYVRVQR QKKILSIRLK VPPTIAQFQY TLDRNTAAE TFKLFNKYRP ETAAEKKERL TKEAAAVAEG KSKQDASPKP YAVKYGLNHV VALIENKKAK LVLIANDVDP I ELVVFLPA LCKKMGVPYA IVKGKARLGT LVNQKTSAVA ALTEVRAEDE AALAKLVSTI DANFADKYDE VKKHWGGGIL GN KAQAKMD KRAKNSDSA

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Macromolecule #11: 60S ribosomal protein L9-A

MacromoleculeName: 60S ribosomal protein L9-A / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 21.605061 KDa
SequenceString:
MKYIQTEQQI EVPEGVTVSI KSRIVKVVGP RGTLTKNLKH IDVTFTKVNN QLIKVAVHNG GRKHVAALRT VKSLVDNMIT GVTKGYKYK MRYVYAHFPI NVNIVEKDGA KFIEVRNFLG DKKIRNVPVR DGVTIEFSTN VKDEIVLSGN SVEDVSQNAA D LQQICRVR NKDIRKFLDG IYVSHKGFIT EDL

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Macromolecule #12: RPL10 isoform 1

MacromoleculeName: RPL10 isoform 1 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 25.410465 KDa
SequenceString: MARRPARCYR YQKNKPYPKS RYNRAVPDSK IRIYDLGKKK ATVDEFPLCV HLVSNELEQL SSEALEAARI CANKYMTTVS GRDAFHLRV RVHPFHVLRI NKMLSCAGAD RLQQGMRGAW GKPHGLAARV DIGQIIFSVR TKDSNKDVVV EGLRRARYKF P GQQKIILS ...String:
MARRPARCYR YQKNKPYPKS RYNRAVPDSK IRIYDLGKKK ATVDEFPLCV HLVSNELEQL SSEALEAARI CANKYMTTVS GRDAFHLRV RVHPFHVLRI NKMLSCAGAD RLQQGMRGAW GKPHGLAARV DIGQIIFSVR TKDSNKDVVV EGLRRARYKF P GQQKIILS KKWGFTNLDR PEYLKKREAG EVKDDGAFVK FLSKKGSLEN NIREFPEYFA AQA

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Macromolecule #13: RPL11B isoform 1

MacromoleculeName: RPL11B isoform 1 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 19.785719 KDa
SequenceString:
MSTKAQNPMR DLKIEKLVLN ISVGESGDRL TRASKVLEQL SGQTPVQSKA RYTVRTFGIR RNEKIAVHVT VRGPKAEEIL ERGLKVKEY QLRDRNFSAT GNFGFGIDEH IDLGIKYDPS IGIFGMDFYV VMNRPGARVT RRKRCKGTVG NSHKTTKEDT V SWFKQKYD ADVLDK

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Macromolecule #14: 60S ribosomal protein L13

MacromoleculeName: 60S ribosomal protein L13 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 22.604164 KDa
SequenceString: MAISKNLPIL KNHFRKHWQE RVKVHFDQAG KKVSRRNARA TRAAKIAPRP LDLLRPVVRA PTVKYNRKVR AGRGFTLAEV KAAGLTAAY ARTIGIAVDH RRQNRNQEIF DANVQRLKEY QSKIIVFPRN GKAPEAEQVL SAAATFPIAQ PATDVEARAV Q DNGESAFR ...String:
MAISKNLPIL KNHFRKHWQE RVKVHFDQAG KKVSRRNARA TRAAKIAPRP LDLLRPVVRA PTVKYNRKVR AGRGFTLAEV KAAGLTAAY ARTIGIAVDH RRQNRNQEIF DANVQRLKEY QSKIIVFPRN GKAPEAEQVL SAAATFPIAQ PATDVEARAV Q DNGESAFR TLRLARSEKK FRGIREKRAR EKAEAEAEKK K

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Macromolecule #15: 60S ribosomal protein L14-A

MacromoleculeName: 60S ribosomal protein L14-A / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 15.195066 KDa
SequenceString:
MSTDSIVKAS NWRLVEVGRV VLIKKGQSAG KLAAIVEIID QKKVLIDGPK AGVPRQAINL GQVVLTPLTF ALPRGARTAT VSKKWAAAA VCEKWAASSW AKKIAQRERR AALTDFERFQ VMVLRKQKRY TVKKALAKA

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Macromolecule #16: 60S ribosomal protein L15-A

MacromoleculeName: 60S ribosomal protein L15-A / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 24.482357 KDa
SequenceString: MGAYKYLEEL QRKKQSDVLR FLQRVRVWEY RQKNVIHRAA RPTRPDKARR LGYKAKQGFV IYRVRVRRGN RKRPVPKGAT YGKPTNQGV NELKYQRSLR ATAEERVGRR AANLRVLNSY WVNQDSTYKY FEVILVDPQH KAIRRDARYN WICDPVHKHR E ARGLTATG ...String:
MGAYKYLEEL QRKKQSDVLR FLQRVRVWEY RQKNVIHRAA RPTRPDKARR LGYKAKQGFV IYRVRVRRGN RKRPVPKGAT YGKPTNQGV NELKYQRSLR ATAEERVGRR AANLRVLNSY WVNQDSTYKY FEVILVDPQH KAIRRDARYN WICDPVHKHR E ARGLTATG KKSRGINKGH KFNNTKAGRR KTWKRQNTLS LWRYRK

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Macromolecule #17: 60S ribosomal protein L16-A

MacromoleculeName: 60S ribosomal protein L16-A / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 22.247227 KDa
SequenceString: MSVEPVVVID GKGHLVGRLA SVVAKQLLNG QKIVVVRAEE LNISGEFFRN KLKYHDFLRK ATAFNKTRGP FHFRAPSRIF YKALRGMVS HKTARGKAAL ERLKVFEGIP PPYDKKKRVV VPQALRVLRL KPGRKYTTLG KLSTSVGWKY EDVVAKLEAK R KVSSAEYY ...String:
MSVEPVVVID GKGHLVGRLA SVVAKQLLNG QKIVVVRAEE LNISGEFFRN KLKYHDFLRK ATAFNKTRGP FHFRAPSRIF YKALRGMVS HKTARGKAAL ERLKVFEGIP PPYDKKKRVV VPQALRVLRL KPGRKYTTLG KLSTSVGWKY EDVVAKLEAK R KVSSAEYY AKKRAFTKKV ASANATAAES DVAKQLAALG Y

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Macromolecule #18: 60S ribosomal protein L17-A

MacromoleculeName: 60S ribosomal protein L17-A / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 20.589518 KDa
SequenceString:
MARYGATSTN PAKSASARGS YLRVSFKNTR ETAQAINGWE LTKAQKYLEQ VLDHQRAIPF RRFNSSIGRT AQGKEFGVTK ARWPAKSVK FVQGLLQNAA ANAEAKGLDA TKLYVSHIQV NQAPKQRRRT YRAHGRINKY ESSPSHIELV VTEKEEAVAK A AEKKVVRL TSRQRGRIAA QKRIAA

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Macromolecule #19: 60S ribosomal protein L18-B

MacromoleculeName: 60S ribosomal protein L18-B / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 20.609252 KDa
SequenceString:
MGIDHTSKQH KRSGHRTAPK SDNVYLKLLV KLYTFLARRT DAPFNKVVLK ALFLSKINRP PVSVSRIARA LKQEGAANKT VVVVGTVTD DARIFEFPKT TVAALRFTAG ARAKIVKAGG ECITLDQLAV RAPKGQNTLI LRGPRNSREA VRHFGMGPHK G KAPRILST GRKFERARGR RRSKGFKV

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Macromolecule #20: 60S ribosomal protein L19-A

MacromoleculeName: 60S ribosomal protein L19-A / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 21.762316 KDa
SequenceString:
MANLRTQKRL AASVVGVGKR KVWLDPNETS EIAQANSRNA IRKLVKNGTI VKKAVTVHSK SRTRAHAQSK REGRHSGYGK RKGTREARL PSQVVWIRRL RVLRRLLAKY RDAGKIDKHL YHVLYKESKG NAFKHKRALV EHIIQAKADA QREKALNEEA E ARRLKNRA ARDRRAQRVA EKRDALLKED A

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Macromolecule #21: 60S ribosomal protein L20-A

MacromoleculeName: 60S ribosomal protein L20-A / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 20.478852 KDa
SequenceString:
MAHFKEYQVI GRRLPTESVP EPKLFRMRIF ASNEVIAKSR YWYFLQKLHK VKKASGEIVS INQINEAHPT KVKNFGVWVR YDSRSGTHN MYKEIRDVSR VAAVETLYQD MAARHRARFR SIHILKVAEI EKTADVKRQY VKQFLTKDLK FPLPHRVQKS T KTFSYKRP STFY

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Macromolecule #22: 60S ribosomal protein L21-A

MacromoleculeName: 60S ribosomal protein L21-A / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 18.279266 KDa
SequenceString:
MGKSHGYRSR TRYMFQRDFR KHGAVHLSTY LKVYKVGDIV DIKANGSIQK GMPHKFYQGK TGVVYNVTKS SVGVIINKMV GNRYLEKRL NLRVEHIKHS KCRQEFLERV KANAAKRAEA KAQGVAVQLK RQPAQPRESR IVSTEGNVPQ TLAPVPYETF I

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Macromolecule #23: 60S ribosomal protein L22-A

MacromoleculeName: 60S ribosomal protein L22-A / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 13.711359 KDa
SequenceString:
MAPNTSRKQK IAKTFTVDVS SPTENGVFDP ASYAKYLIDH IKVEGAVGNL GNAVTVTEDG TVVTVVSTAK FSGKYLKYLT KKYLKKNQL RDWIRFVSTK TNEYRLAFYQ VTPEEDEEED EE

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Macromolecule #24: 60S ribosomal protein L23-B

MacromoleculeName: 60S ribosomal protein L23-B / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 14.49395 KDa
SequenceString:
MSGNGAQGTK FRISLGLPVG AIMNCADNSG ARNLYIIAVK GSGSRLNRLP AASLGDMVMA TVKKGKPELR KKVMPAIVVR QAKSWRRRD GVFLYFEDNA GVIANPKGEM KGSAITGPVG KECADLWPRV ASNSGVVV

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Macromolecule #25: RPL24A isoform 1

MacromoleculeName: RPL24A isoform 1 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 17.661717 KDa
SequenceString:
MKVEIDSFSG AKIYPGRGTL FVRGDSKIFR FQNSKSASLF KQRKNPRRIA WTVLFRKHHK KGITEEVAKK RSRKTVKAQR PITGASLDL IKERRSLKPE VRKANREEKL KANKEKKKAE KAARKAEKAK SAGTQSSKFS KQQAKGAFQK VAATSR

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Macromolecule #26: 60S ribosomal protein L25

MacromoleculeName: 60S ribosomal protein L25 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 15.787612 KDa
SequenceString:
MAPSAKATAA KKAVVKGTNG KKALKVRTSA TFRLPKTLKL ARAPKYASKA VPHYNRLDSY KVIEQPITSE TAMKKVEDGN ILVFQVSMK ANKYQIKKAV KELYEVDVLK VNTLVRPNGT KKAYVRLTAD YDALDIANRI GYI

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Macromolecule #27: 60S ribosomal protein L26-A

MacromoleculeName: 60S ribosomal protein L26-A / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 14.265784 KDa
SequenceString:
MAKQSLDVSS DRRKARKAYF TAPSSQRRVL LSAPLSKELR AQYGIKALPI RRDDEVLVVR GSKKGQEGKI SSVYRLKFAV QVDKVTKEK VNGASVPINL HPSKLVITKL HLDKDRKALI QRKGGKLE

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Macromolecule #28: 60S ribosomal protein L27

MacromoleculeName: 60S ribosomal protein L27 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 15.56836 KDa
SequenceString:
MAKFLKAGKV AVVVRGRYAG KKVVIVKPHD EGSKSHPFGH ALVAGIERYP LKVTKKHGAK KVAKRTKIKP FIKVVNYNHL LPTRYTLDV EAFKSVVSTE TFEQPSQREE AKKVVKKAFE ERHQAGKNQW FFSKLRF

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Macromolecule #29: 60S ribosomal protein L28

MacromoleculeName: 60S ribosomal protein L28 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 16.761666 KDa
SequenceString:
MPSRFTKTRK HRGHVSAGKG RIGKHRKHPG GRGMAGGQHH HRINMDKYHP GYFGKVGMRY FHKQQAHFWK PVLNLDKLWT LIPEDKRDQ YLKSASKETA PVIDTLAAGY GKILGKGRIP NVPVIVKARF VSKLAEEKIR AAGGVVELIA

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Macromolecule #30: RPL29 isoform 1

MacromoleculeName: RPL29 isoform 1 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 6.691884 KDa
SequenceString:
MAKSKNHTAH NQTRKAHRNG IKKPKTYKYP SLKGVDPKFR RNHKHALHGT AKALAAAKK

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Macromolecule #31: 60S ribosomal protein L30

MacromoleculeName: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 11.430364 KDa
SequenceString:
MAPVKSQESI NQKLALVIKS GKYTLGYKST VKSLRQGKSK LIIIAANTPV LRKSELEYYA MLSKTKVYYF QGGNNELGTA VGKLFRVGV VSILEAGDSD ILTTLA

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Macromolecule #32: 60S ribosomal protein L31-A

MacromoleculeName: 60S ribosomal protein L31-A / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 12.980158 KDa
SequenceString:
MAGLKDVVTR EYTINLHKRL HGVSFKKRAP RAVKEIKKFA KLHMGTDDVR LAPELNQAIW KRGVKGVEYR LRLRISRKRN EEEDAKNPL FSYVEPVLVA SAKGLQTVVV EEDA

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Macromolecule #33: RPL32 isoform 1

MacromoleculeName: RPL32 isoform 1 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 14.809441 KDa
SequenceString:
MASLPHPKIV KKHTKKFKRH HSDRYHRVAE NWRKQKGIDS VVRRRFRGNI SQPKIGYGSN KKTKFLSPSG HKTFLVANVK DLETLTMHT KTYAAEIAHN ISAKNRVVIL ARAKALGIKV TNPKGRLALE A

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Macromolecule #34: 60S ribosomal protein L33-A

MacromoleculeName: 60S ribosomal protein L33-A / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 12.17713 KDa
SequenceString:
MAESHRLYVK GKHLSYQRSK RVNNPNVSLI KIEGVATPQD AQFYLGKRIA YVYRASKEVR GSKIRVMWGK VTRTHGNSGV VRATFRNNL PAKTFGASVR IFLYPSNI

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Macromolecule #35: 60S ribosomal protein L34-A

MacromoleculeName: 60S ribosomal protein L34-A / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 13.673196 KDa
SequenceString:
MAQRVTFRRR NPYNTRSNKI KVVKTPGGIL RAQHVKKLAT RPKCGDCGSA LQGISTLRPR QYATVSKTHK TVSRAYGGSR CANCVKERI IRAFLIEEQK IVKKVVKEQT EAAKKSEKKA KK

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Macromolecule #36: 60S ribosomal protein L35-A

MacromoleculeName: 60S ribosomal protein L35-A / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 13.94264 KDa
SequenceString:
MAGVKAYELR TKSKEQLASQ LVDLKKELAE LKVQKLSRPS LPKIKTVRKS IACVLTVINE QQREAVRQLY KGKKYQPKDL RAKKTRALR RALTKFEASQ VTEKQRKKQI AFPQRKYAIK A

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Macromolecule #37: 60S ribosomal protein L36-A

MacromoleculeName: 60S ribosomal protein L36-A / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 11.151259 KDa
SequenceString:
MTVKTGIAIG LNKGKKVTSM TPAPKISYKK GAASNRTKFV RSLVREIAGL SPYERRLIDL IRNSGEKRAR KVAKKRLGSF TRAKAKVEE MNNIIAASRR H

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Macromolecule #38: Ribosomal protein L37

MacromoleculeName: Ribosomal protein L37 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 9.877395 KDa
SequenceString:
MGKGTPSFGK RHNKSHTLCN RCGRRSFHVQ KKTCSSCGYP AAKTRSYNWG AKAKRRHTTG TGRMRYLKHV SRRFKNGFQT GSASKASA

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Macromolecule #39: RPL38 isoform 1

MacromoleculeName: RPL38 isoform 1 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 8.845561 KDa
SequenceString:
MAREITDIKQ FLELTRRADV KTATVKINKK LNKAGKPFRQ TKFKVRGSSS LYTLVINDAG KAKKLIQSLP PTLKVNRL

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Macromolecule #40: 60S ribosomal protein L39

MacromoleculeName: 60S ribosomal protein L39 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 6.35864 KDa
SequenceString:
MAAQKSFRIK QKMAKAKKQN RPLPQWIRLR TNNTIRYNAK RRNWRRTKMN I

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Macromolecule #41: Ubiquitin-60S ribosomal protein L40

MacromoleculeName: Ubiquitin-60S ribosomal protein L40 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 14.583077 KDa
SequenceString:
MQIFVKTLTG KTITLEVESS DTIDNVKSKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGIIEP SLKALASKY NCDKSVCRKC YARLPPRATN CRKRKCGHTN QLRPKKKLK

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Macromolecule #42: 60S ribosomal protein L41-A

MacromoleculeName: 60S ribosomal protein L41-A / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 3.354243 KDa
SequenceString:
MRAKWRKKRT RRLKRKRRKV RARSK

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Macromolecule #43: 60S ribosomal protein L42-A

MacromoleculeName: 60S ribosomal protein L42-A / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 12.246658 KDa
SequenceString:
MVNVPKTRKT YCKGKTCRKH TQHKVTQYKA GKASLFAQGK RRYDRKQSGF GGQTKPVFHK KAKTTKKVVL RLECVKCKTR AQLTLKRCK HFELGGEKKQ KGQALQF

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Macromolecule #44: 60S ribosomal protein L43-A

MacromoleculeName: 60S ribosomal protein L43-A / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 10.112952 KDa
SequenceString:
MAKRTKKVGI TGKYGVRYGS SLRRQVKKLE IQQHARYDCS FCGKKTVKRG AAGIWTCSCC KKTVAGGAYT VSTAAAATVR STIRRLREM VEA

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Macromolecule #45: RPP0 isoform 1

MacromoleculeName: RPP0 isoform 1 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 33.749121 KDa
SequenceString: MGGIREKKAE YFAKLREYLE EYKSLFVVGV DNVSSQQMHE VRKELRGRAV VLMGKNTMVR RAIRGFLSDL PDFEKLLPFV KGNVGFVFT NEPLTEIKNV IVSNRVAAPA RAGAVAPEDI WVRAVNTGME PGKTSFFQAL GVPTKIARGT IEIVSDVKVV D AGNKVGQS ...String:
MGGIREKKAE YFAKLREYLE EYKSLFVVGV DNVSSQQMHE VRKELRGRAV VLMGKNTMVR RAIRGFLSDL PDFEKLLPFV KGNVGFVFT NEPLTEIKNV IVSNRVAAPA RAGAVAPEDI WVRAVNTGME PGKTSFFQAL GVPTKIARGT IEIVSDVKVV D AGNKVGQS EASLLNLLNI SPFTFGLTVV QVYDNGQVFP SSILDITDEE LVSHFVSAVS TIASISLAIG YPTLPSVGHT LI NNYKDLL AVAIAASYHY PEIEDLVDRI ENPEKYAAAA PAATSAASGD AAPAEEAAAE EEEESDDDMG FGLFD

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Macromolecule #46: RPL12A isoform 1

MacromoleculeName: RPL12A isoform 1 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 17.850621 KDa
SequenceString:
MPPKFDPNEV KYLYLRAVGG EVGASAALAP KIGPLGLSPK KVGEDIAKAT KEFKGIKVTV QLKIQNRQAA ASVVPSASSL VITALKEPP RDRKKDKNVK HSGNIQLDEI IEIARQMRDK SFGRTLASVT KEILGTAQSV GCRVDFKNPH DIIEGINAGE I EIPEN

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Macromolecule #48: 40S ribosomal protein S0

MacromoleculeName: 40S ribosomal protein S0 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 28.05133 KDa
SequenceString: MSLPATFDLT PEDAQLLLAA NTHLGARNVQ VHQEPYVFNA RPDGVHVINV GKTWEKLVLA ARIIAAIPNP EDVVAISSRT FGQRAVLKF AAHTGATPIA GRFTPGSFTN YITRSFKEPR LVIVTDPRSD AQAIKEASYV NIPVIALTDL DSPSEFVDVA I PCNNRGKH ...String:
MSLPATFDLT PEDAQLLLAA NTHLGARNVQ VHQEPYVFNA RPDGVHVINV GKTWEKLVLA ARIIAAIPNP EDVVAISSRT FGQRAVLKF AAHTGATPIA GRFTPGSFTN YITRSFKEPR LVIVTDPRSD AQAIKEASYV NIPVIALTDL DSPSEFVDVA I PCNNRGKH SIGLIWYLLA REVLRLRGAL VDRTQPWSIM PDLYFYRDPE EVEQQVAEEA TTEEAGEEEA KEEVTEEQAE AT EWAEENA DNVEW

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Macromolecule #49: RPS1A isoform 1

MacromoleculeName: RPS1A isoform 1 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 28.798467 KDa
SequenceString: MAVGKNKRLS KGKKGQKKRV VDPFTRKEWF DIKAPSTFEN RNVGKTLVNK STGLKSASDA LKGRVVEVCL ADLQGSEDHS FRKIKLRVD EVQGKNLLTN FHGMDFTTDK LRSMVRKWQT LIEANVTVKT SDDYVLRIFA IAFTRKQANQ VKRHSYAQSS H IRAIRKVI ...String:
MAVGKNKRLS KGKKGQKKRV VDPFTRKEWF DIKAPSTFEN RNVGKTLVNK STGLKSASDA LKGRVVEVCL ADLQGSEDHS FRKIKLRVD EVQGKNLLTN FHGMDFTTDK LRSMVRKWQT LIEANVTVKT SDDYVLRIFA IAFTRKQANQ VKRHSYAQSS H IRAIRKVI SEILTKEVQG STLAQLTSKL IPEVINKEIE NATKDIFPLQ NIHVRKVKLL KQPKFDVGAL MALHGEGSGE EK GKKVTGF KDEVLETV

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Macromolecule #50: RPS2 isoform 1

MacromoleculeName: RPS2 isoform 1 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 27.490826 KDa
SequenceString: MSAPEAQQQK RGGFGGRNRG RPNRRGPRNT EEKGWVPVTK LGRLVKAGKI TTIEEIFLHS LPVKEFQIID TLLPGLQDEV MNIKPVQKQ TRAGQRTRFK AVVVVGDSNG HVGLGIKTAK EVAGAIRAGI IIAKLSVIPI RRGYWGTNLG QPHSLATKTT G KCGSVTVR ...String:
MSAPEAQQQK RGGFGGRNRG RPNRRGPRNT EEKGWVPVTK LGRLVKAGKI TTIEEIFLHS LPVKEFQIID TLLPGLQDEV MNIKPVQKQ TRAGQRTRFK AVVVVGDSNG HVGLGIKTAK EVAGAIRAGI IIAKLSVIPI RRGYWGTNLG QPHSLATKTT G KCGSVTVR LIPAPRGSGI VASPAVKKLL QLAGVEDVYT QSNGKTRTLE NTLKAAFVAI GNTYGFLTPN LWAEQPLPVS PL DIYSDEA SAQKKRF

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Macromolecule #51: RPS3 isoform 1

MacromoleculeName: RPS3 isoform 1 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 26.542789 KDa
SequenceString: MVALISKKRK LVADGVFYAE LNEFFTRELA EEGYSGVEVR VTPTKTEVII RATRTQDVLG ENGRRINELT LLVQKRFKYA PGTIVLYAE RVQDRGLSAV AQAESMKFKL LNGLAIRRAA YGVVRYVMES GAKGCEVVVS GKLRAARAKA MKFADGFLIH S GQPVNDFI ...String:
MVALISKKRK LVADGVFYAE LNEFFTRELA EEGYSGVEVR VTPTKTEVII RATRTQDVLG ENGRRINELT LLVQKRFKYA PGTIVLYAE RVQDRGLSAV AQAESMKFKL LNGLAIRRAA YGVVRYVMES GAKGCEVVVS GKLRAARAKA MKFADGFLIH S GQPVNDFI DTATRHVLMR QGVLGIKVKI MRDPAKSRTG PKALPDAVTI IEPKEEEPIL APSVKDYRPA EETEAQAEPV EA

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Macromolecule #52: 40S ribosomal protein S4

MacromoleculeName: 40S ribosomal protein S4 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 29.46933 KDa
SequenceString: MARGPKKHLK RLAAPHHWLL DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN RLKYALNGRE VKAILMQRHV KVDGKVRTDT TYPAGFMDV ITLDATNENF RLVYDVKGRF AVHRITDEEA SYKLGKVKKV QLGKKGVPYV VTHDGRTIRY PDPNIKVNDT V KIDLASGK ...String:
MARGPKKHLK RLAAPHHWLL DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN RLKYALNGRE VKAILMQRHV KVDGKVRTDT TYPAGFMDV ITLDATNENF RLVYDVKGRF AVHRITDEEA SYKLGKVKKV QLGKKGVPYV VTHDGRTIRY PDPNIKVNDT V KIDLASGK ITDFIKFDAG KLVYVTGGRN LGRIGTIVHK ERHDGGFDLV HIKDSLDNTF VTRLNNVFVI GEQGKPYISL PK GKGIKLS IAEERDRRRA QQGL

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Macromolecule #53: Rps5p

MacromoleculeName: Rps5p / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 25.0726 KDa
SequenceString: MSDTEAPVEV QEDFEVVEEF TPVVLATPIP EEVQQAQTEI KLFNKWSFEE VEVKDASLVD YVQVRQPIFV AHTAGRYANK RFRKAQCPI IERLTNSLMM NGRNNGKKLK AVRIIKHTLD IINVLTDQNP IQVVVDAITN TGPREDTTRV GGGGAARRQA V DVSPLRRV ...String:
MSDTEAPVEV QEDFEVVEEF TPVVLATPIP EEVQQAQTEI KLFNKWSFEE VEVKDASLVD YVQVRQPIFV AHTAGRYANK RFRKAQCPI IERLTNSLMM NGRNNGKKLK AVRIIKHTLD IINVLTDQNP IQVVVDAITN TGPREDTTRV GGGGAARRQA V DVSPLRRV NQAIALLTIG AREAAFRNIK TIAETLAEEL INAAKGSSTS YAIKKKDELE RVAKSNR

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Macromolecule #54: 40S ribosomal protein S6

MacromoleculeName: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 27.054486 KDa
SequenceString: MKLNISYPVN GSQKTFEIDD EHRIRVFFDK RIGQEVDGEA VGDEFKGYVF KISGGNDKQG FPMKQGVLLP TRIKLLLTKN VSCYRPRRD GERKRKSVRG AIVGPDLAVL ALVIVKKGEQ ELEGLTDTTV PKRLGPKRAN NIRKFFGLSK EDDVRDFVIR R EVTKGEKT ...String:
MKLNISYPVN GSQKTFEIDD EHRIRVFFDK RIGQEVDGEA VGDEFKGYVF KISGGNDKQG FPMKQGVLLP TRIKLLLTKN VSCYRPRRD GERKRKSVRG AIVGPDLAVL ALVIVKKGEQ ELEGLTDTTV PKRLGPKRAN NIRKFFGLSK EDDVRDFVIR R EVTKGEKT YTKAPKIQRL VTPQRLQRKR HQRALKVRNA QAQREAAAEY AQLLAKRLSE RKAEKAEIRK RRASSLKA

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Macromolecule #55: 40S ribosomal protein S7

MacromoleculeName: 40S ribosomal protein S7 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 21.658209 KDa
SequenceString:
MSAPQAKILS QAPTELELQV AQAFVELENS SPELKAELRP LQFKSIREID VAGGKKALAI FVPVPSLAGF HKVQTKLTRE LEKKFQDRH VIFLAERRIL PKPSRTSRQV QKRPRSRTLT AVHDKILEDL VFPTEIVGKR VRYLVGGNKI QKVLLDSKDV Q QIDYKLES FQAVYNKLTG KQIVFEIPSE TH

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Macromolecule #56: RPS8A isoform 1

MacromoleculeName: RPS8A isoform 1 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 22.537803 KDa
SequenceString: MGISRDSRHK RSATGAKRAQ FRKKRKFELG RQPANTKIGA KRIHSVRTRG GNKKYRALRI ETGNFSWASE GISKKTRIAG VVYHPSNNE LVRTNTLTKA AIVQIDATPF RQWFEAHYGQ TLGKKKNVKE EETVAKSKNA ERKWAARAAS AKIESSVESQ F SAGRLYAC ...String:
MGISRDSRHK RSATGAKRAQ FRKKRKFELG RQPANTKIGA KRIHSVRTRG GNKKYRALRI ETGNFSWASE GISKKTRIAG VVYHPSNNE LVRTNTLTKA AIVQIDATPF RQWFEAHYGQ TLGKKKNVKE EETVAKSKNA ERKWAARAAS AKIESSVESQ F SAGRLYAC ISSRPGQSGR CDGYILEGEE LAFYLRRLTA KK

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Macromolecule #57: 40S ribosomal protein S9-A

MacromoleculeName: 40S ribosomal protein S9-A / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 22.487893 KDa
SequenceString: MPRAPRTYSK TYSTPKRPYE SSRLDAELKL AGEFGLKNKK EIYRISFQLS KIRRAARDLL TRDEKDPKRL FEGNALIRRL VRVGVLSED KKKLDYVLAL KVEDFLERRL QTQVYKLGLA KSVHHARVLI TQRHIAVGKQ IVNIPSFMVR LDSEKHIDFA P TSPFGGAR ...String:
MPRAPRTYSK TYSTPKRPYE SSRLDAELKL AGEFGLKNKK EIYRISFQLS KIRRAARDLL TRDEKDPKRL FEGNALIRRL VRVGVLSED KKKLDYVLAL KVEDFLERRL QTQVYKLGLA KSVHHARVLI TQRHIAVGKQ IVNIPSFMVR LDSEKHIDFA P TSPFGGAR PGRVARRNAA RKAEASGEAA DEADEADEE

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Macromolecule #58: 40S ribosomal protein S10-A

MacromoleculeName: 40S ribosomal protein S10-A / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 12.757445 KDa
SequenceString:
MLMPKEDRNK IHQYLFQEGV VVAKKDFNQA KHEEIDTKNL YVIKALQSLT SKGYVKTQFS WQYYYYTLTE EGVEYLREYL NLPEHIVPG TYIQERNPTQ RPQRRY

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Macromolecule #59: 40S ribosomal protein S11-B

MacromoleculeName: 40S ribosomal protein S11-B / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 17.785934 KDa
SequenceString:
MSTELTVQSE RAFQKQPHIF NNPKVKTSKR TKRWYKNAGL GFKTPKTAIE GSYIDKKCPF TGLVSIRGKI LTGTVVSTKM HRTIVIRRA YLHYIPKYNR YEKRHKNVPV HVSPAFRVQV GDIVTVGQCR PISKTVRFNV VKVSAAAGKA NKQFAKF

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Macromolecule #60: 40S ribosomal protein S12

MacromoleculeName: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 15.488631 KDa
SequenceString:
MSDVEEVVEV QEETVVEQTA EVTIEDALKV VLRTALVHDG LARGLRESTK ALTRGEALLV VLVSSVTEAN IIKLVEGLAN DPENKVPLI KVADAKQLGE WAGLGKIDRE GNARKVVGAS VVVVKNWGAE TDELSMIMEH FSQQ

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Macromolecule #61: 40S ribosomal protein S13

MacromoleculeName: 40S ribosomal protein S13 / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 17.059945 KDa
SequenceString:
MGRMHSAGKG ISSSAIPYSR NAPAWFKLSS ESVIEQIVKY ARKGLTPSQI GVLLRDAHGV TQARVITGNK IMRILKSNGL APEIPEDLY YLIKKAVSVR KHLERNRKDK DAKFRLILIE SRIHRLARYY RTVAVLPPNW KYESATASAL VN

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Macromolecule #62: 40S ribosomal protein S14-B

MacromoleculeName: 40S ribosomal protein S14-B / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 14.675771 KDa
SequenceString:
MANDLVQARD NSQVFGVARI YASFNDTFVH VTDLSGKETI ARVTGGMKVK ADRDESSPYA AMLAAQDVAA KCKEVGITAV HVKIRATGG TRTKTPGPGG QAALRALARS GLRIGRIEDV TPVPSDSTRK KGGRRGRRL

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Macromolecule #63: RPS15 isoform 1

MacromoleculeName: RPS15 isoform 1 / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 16.031907 KDa
SequenceString:
MSQAVNAKKR VFKTHSYRGV DLEKLLEMST EDFVKLAPAR VRRRFARGMT SKPAGFMKKL RAAKLAAPEN EKPAPVRTHM RNMIIVPEM IGSVVGIYNG KAFNQVEIRP EMLGHYLGEF SITYTPVRHG RAGATTSRFI PLK

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Macromolecule #64: 40S ribosomal protein S16-A

MacromoleculeName: 40S ribosomal protein S16-A / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 15.87749 KDa
SequenceString:
MSAVPSVQTF GKKKSATAVA HVKAGKGLIK VNGSPITLVE PEILRFKVYE PLLLVGLDKF SNIDIRVRVT GGGHVSQVYA IRQAIAKGL VAYHQKYVDE QSKNELKKAF TSYDRTLLIA DSRRPEPKKF GGKGARSRFQ KSYR

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Macromolecule #65: 40S ribosomal protein S17-A

MacromoleculeName: 40S ribosomal protein S17-A / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 15.820413 KDa
SequenceString:
MGRVRTKTVK RASKALIERY YPKLTLDFQT NKRLCDEIAT IQSKRLRNKI AGYTTHLMKR IQKGPVRGIS FKLQEEERER KDQYVPEVS ALDLSRSNGV LNVDNQTSDL VKSLGLKLPL SVINVSAQRD RRYRKRV

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Macromolecule #66: 40S ribosomal protein S18-B

MacromoleculeName: 40S ribosomal protein S18-B / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 17.071641 KDa
SequenceString:
MSLVVQEQGS FQHILRLLNT NVDGNIKIVY ALTTIKGVGR RYSNLVCKKA DVDLHKRAGE LTQEELERIV QIMQNPTHYK IPAWFLNRQ NDITDGKDYH TLANNVESKL RDDLERLKKI RAHRGIRHFW GLRVRGQHTK TTGRRRA

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Macromolecule #67: 40S ribosomal protein S19-A

MacromoleculeName: 40S ribosomal protein S19-A / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 15.942125 KDa
SequenceString:
MPGVSVRDVA AQDFINAYAS FLQRQGKLEV PGYVDIVKTS SGNEMPPQDA EGWFYKRAAS VARHIYMRKQ VGVGKLNKLY GGAKSRGVR PYKHIDASGS INRKVLQALE KIGIVEISPK GGRRISENGQ RDLDRIAAQT LEEDE

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Macromolecule #68: RPS20 isoform 1

MacromoleculeName: RPS20 isoform 1 / type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 13.929044 KDa
SequenceString:
MSDFQKEKVE EQEQQQQQII KIRITLTSTK VKQLENVSSN IVKNAEQHNL VKKGPVRLPT KVLKISTRKT PNGEGSKTWE TYEMRIHKR YIDLEAPVQI VKRITQITIE PGVDVEVVVA SN

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Macromolecule #69: 40S ribosomal protein S21-A

MacromoleculeName: 40S ribosomal protein S21-A / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 9.758829 KDa
SequenceString:
MENDKGQLVE LYVPRKCSAT NRIIKADDHA SVQINVAKVD EEGRAIPGEY VTYALSGYVR SRGESDDSLN RLAQNDGLLK NVWSYSR

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Macromolecule #70: RPS22A isoform 1

MacromoleculeName: RPS22A isoform 1 / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 14.650062 KDa
SequenceString:
MTRSSVLADA LNAINNAEKT GKRQVLIRPS SKVIIKFLQV MQKHGYIGEF EYIDDHRSGK IVVQLNGRLN KCGVISPRFN VKIGDIEKW TANLLPARQF GYVILTTSAG IMDHEEARRK HVSGKILGFV Y

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Macromolecule #71: 40S ribosomal protein S23

MacromoleculeName: 40S ribosomal protein S23 / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 16.073896 KDa
SequenceString:
MGKGKPRGLN SARKLRVHRR NNRWAENNYK KRLLGTAFKS SPFGGSSHAK GIVLEKLGIE SKQPNSAIRK CVRVQLIKNG KKVTAFVPN DGCLNFVDEN DEVLLAGFGR KGKAKGDIPG VRFKVVKVSG VSLLALWKEK KEKPRS

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Macromolecule #72: 40S ribosomal protein S24

MacromoleculeName: 40S ribosomal protein S24 / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 15.362848 KDa
SequenceString:
MSDAVTIRTR KVISNPLLAR KQFVVDVLHP NRANVSKDEL REKLAEVYKA EKDAVSVFGF RTQFGGGKSV GFGLVYNSVA EAKKFEPTY RLVRYGLAEK VEKASRQQRK QKKNRDKKIF GTGKRLAKKV ARRNAD

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Macromolecule #73: RPS25A isoform 1

MacromoleculeName: RPS25A isoform 1 / type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 12.067272 KDa
SequenceString:
MPPKQQLSKA AKAAAALAGG KKSKKKWSKK SMKDRAQHAV ILDQEKYDRI LKEVPTYRYV SVSVLVDRLK IGGSLARIAL RHLEKEGII KPISKHSKQA IYTRATASE

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Macromolecule #74: RPS26B isoform 1

MacromoleculeName: RPS26B isoform 1 / type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 13.480886 KDa
SequenceString:
MPKKRASNGR NKKGRGHVKP VRCVNCSKSI PKDKAIKRMA IRNIVEAAAV RDLSEASVYP EYALPKTYNK LHYCVSCAIH ARIVRVRSR EDRKNRAPPQ RPRFNRDNKV SPAAAAKKAL

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Macromolecule #75: 40S ribosomal protein S27-A

MacromoleculeName: 40S ribosomal protein S27-A / type: protein_or_peptide / ID: 75 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 8.893391 KDa
SequenceString:
MVLVQDLLHP TAASEARKHK LKTLVQGPRS YFLDVKCPGC LNITTVFSHA QTAVTCESCS TILCTPTGGK AKLSEGTSFR RK

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Macromolecule #76: RPS28A isoform 1

MacromoleculeName: RPS28A isoform 1 / type: protein_or_peptide / ID: 76 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 7.605847 KDa
SequenceString:
MDNKTPVTLA KVIKVLGRTG SRGGVTQVRV EFLEDTSRTI VRNVKGPVRE NDILVLMESE REARRLR

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Macromolecule #77: RPS29A isoform 1

MacromoleculeName: RPS29A isoform 1 / type: protein_or_peptide / ID: 77 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 6.675723 KDa
SequenceString:
MAHENVWFSH PRRYGKGSRQ CRVCSSHTGL IRKYGLNICR QCFREKANDI GFNKFR

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Macromolecule #78: 40S ribosomal protein S30

MacromoleculeName: 40S ribosomal protein S30 / type: protein_or_peptide / ID: 78 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 7.137541 KDa
SequenceString:
MAKVHGSLAR AGKVKSQTPK VEKTEKPKKP KGRAYKRLLY TRRFVNVTLV NGKRRMNPGP SVQ

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Macromolecule #79: Ubiquitin-40S ribosomal protein S31

MacromoleculeName: Ubiquitin-40S ribosomal protein S31 / type: protein_or_peptide / ID: 79 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 17.254227 KDa
SequenceString:
MQIFVKTLTG KTITLEVESS DTIDNVKSKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGGKKR KKKVYTTPK KIKHKHKKVK LAVLSYYKVD AEGKVTKLRR ECSNPTCGAG VFLANHKDRL YCGKCHSVYK VNA

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Macromolecule #80: Guanine nucleotide-binding protein subunit beta-like protein

MacromoleculeName: Guanine nucleotide-binding protein subunit beta-like protein
type: protein_or_peptide / ID: 80 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 34.841219 KDa
SequenceString: MASNEVLVLR GTLEGHNGWV TSLATSAGQP NLLLSASRDK TLISWKLTGD DQKFGVPVRS FKGHSHIVQD CTLTADGAYA LSASWDKTL RLWDVATGET YQRFVGHKSD VMSVDIDKKA SMIISGSRDK TIKVWTIKGQ CLATLLGHND WVSQVRVVPN E KADDDSVT ...String:
MASNEVLVLR GTLEGHNGWV TSLATSAGQP NLLLSASRDK TLISWKLTGD DQKFGVPVRS FKGHSHIVQD CTLTADGAYA LSASWDKTL RLWDVATGET YQRFVGHKSD VMSVDIDKKA SMIISGSRDK TIKVWTIKGQ CLATLLGHND WVSQVRVVPN E KADDDSVT IISAGNDKMV KAWNLNQFQI EADFIGHNSN INTLTASPDG TLIASAGKDG EIMLWNLAAK KAMYTLSAQD EV FSLAFSP NRYWLAAATA TGIKVFSLDP QYLVDDLRPE FAGYSKAAEP HAVSLAWSAD GQTLFAGYTD NVIRVWQVMT AN

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Macromolecule #84: Eukaryotic translation initiation factor 5A

MacromoleculeName: Eukaryotic translation initiation factor 5A / type: protein_or_peptide / ID: 84 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 17.222406 KDa
SequenceString:
MSDEEHTFET ADAGSSATYP MQCSALRKNG FVVIKSRPCK IVDMSTSKTG (5CT)HGHAKVHLV AIDIFTGKKL EDLSPS THN MEVPVVKRNE YQLLDIDDGF LSLMNMDGDT KDDVKAPEGE LGDSLQTAFD EGKDLMVTII SAMGEEAAIS FKEAART D

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Macromolecule #85: Ribosome-interacting GTPase 1

MacromoleculeName: Ribosome-interacting GTPase 1 / type: protein_or_peptide / ID: 85 / Details: Chromasomally TAP tagged / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 45.470316 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MSTTVEKIKA IEDEMARTQK NKATSFHLGQ LKAKLAKLRR ELLTSASSGS GGGAGIGFDV ARTGVASVGF VGFPSVGKST LLSKLTGTE SEAAEYEFTT LVTVPGVIRY KGAKIQMLDL PGIIDGAKDG RGRGKQVIAV ARTCNLLFII LDVNKPLHHK Q IIEKELEG ...String:
MSTTVEKIKA IEDEMARTQK NKATSFHLGQ LKAKLAKLRR ELLTSASSGS GGGAGIGFDV ARTGVASVGF VGFPSVGKST LLSKLTGTE SEAAEYEFTT LVTVPGVIRY KGAKIQMLDL PGIIDGAKDG RGRGKQVIAV ARTCNLLFII LDVNKPLHHK Q IIEKELEG VGIRLNKTPP DILIKKKEKG GISITNTVPL THLGNDEIRA VMSEYRINSA EIAFRCDATV DDLIDVLEAS SR RYMPAIY VLNKIDSLSI EELELLYRIP NAVPISSGQD WNLDELLQVM WDRLNLVRIY TKPKGQIPDF TDPVVLRSDR CSV KDFCNQ IHKSLVDDFR NALVYGSSVK HQPQYVGLSH ILEDEDVVTI LKKKRRWKKN FIAVSAANRF KKISSSGALD YDIP TTASE NLYFQ

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Macromolecule #86: Translation machinery-associated protein 46

MacromoleculeName: Translation machinery-associated protein 46 / type: protein_or_peptide / ID: 86 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 39.592172 KDa
SequenceString: MPPKKGKQAQ AAGKKKDNVD KTFGMKNKNR STKVQKYIKQ VQSQSDPKKE EMRLKKLEEK KRREAEEAER RALFNPVADQ RVRAGVDPK SMVCALFKLG NCNKGAKCKF SHDLNVGRRM EKKDLYQDTR SEKENDTMDN WDEEKLRKVI LSKHGNPKTT T DKVCKYFI ...String:
MPPKKGKQAQ AAGKKKDNVD KTFGMKNKNR STKVQKYIKQ VQSQSDPKKE EMRLKKLEEK KRREAEEAER RALFNPVADQ RVRAGVDPK SMVCALFKLG NCNKGAKCKF SHDLNVGRRM EKKDLYQDTR SEKENDTMDN WDEEKLRKVI LSKHGNPKTT T DKVCKYFI EAVENGKYGW FWICPNGGDK CMYRHSLPEG FVLKTNEQKR LERESLEKQP KITLEEFIET ERGKLDKSKL TP ITIANFA QWKKDHVIAK INAEKKLSSK RKPTGREIIL KMSAENKSFE TDNADMPDDV TQGSAWDLTE FTDALKKADH QDD GGIKDY GDGSNPTFDI KKANSATLA

+
Macromolecule #87: 60S ribosomal protein L1

MacromoleculeName: 60S ribosomal protein L1 / type: protein_or_peptide / ID: 87
Details: Only show the backbone of the protein due to the poor density
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 17.889996 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)

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Macromolecule #88: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 88 / Number of copies: 286 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #89: ASPARTIC ACID

MacromoleculeName: ASPARTIC ACID / type: ligand / ID: 89 / Number of copies: 1 / Formula: ASP
Molecular weightTheoretical: 133.103 Da
Chemical component information

ChemComp-ASP:
ASPARTIC ACID / Aspartic acid

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Macromolecule #90: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 90 / Number of copies: 8 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
Component:
ConcentrationFormula
20.0 mMTris-HClTris
140.0 mMKCl
1.5 mMMgCl2
1.0 %TritonX100
1.0 mMDTT
100.0 ug/mlCycloheximide
GridModel: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Specialist opticsPhase plate: OTHER
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Average electron dose: 30.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

4v88

Initial angle assignmentType: ANGULAR RECONSTITUTION
Final angle assignmentType: ANGULAR RECONSTITUTION
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.23 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 95380
FSC plot (resolution estimation)

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