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- EMDB-23972: Mtb 70SIC in complex with MtbEttA at Trans_R1 state -

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Basic information

Entry
Database: EMDB / ID: EMD-23972
TitleMtb 70SIC in complex with MtbEttA at Trans_R1 state
Map data
Sample
  • Complex: The complex of Mtb 70SIC with MtbEttA at ATP-hydrolysis transition state (Trans_R1)
    • Protein or peptide: x 50 types
    • RNA: x 5 types
  • Ligand: x 5 types
Keywordsmycobacterium tuberculosis / ABCF ribosome complex / antibiotic / RIBOSOME
Function / homology
Function and homology information


negative regulation of translational elongation / Hydrolases; Acting on acid anhydrides; In phosphorus-containing anhydrides / peptidoglycan-based cell wall / regulation of translation / large ribosomal subunit / ribosome binding / transferase activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit ...negative regulation of translational elongation / Hydrolases; Acting on acid anhydrides; In phosphorus-containing anhydrides / peptidoglycan-based cell wall / regulation of translation / large ribosomal subunit / ribosome binding / transferase activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / 5S rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / chromatin extrusion motor activity / ATP-dependent H2AZ histone chaperone activity / ATP-dependent H3-H4 histone complex chaperone activity / cohesin loader activity / DNA clamp loader activity / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / response to antibiotic / mRNA binding / ATP hydrolysis activity / RNA binding / zinc ion binding / ATP binding / metal ion binding / plasma membrane / cytosol / cytoplasm
Similarity search - Function
Energy-dependent translational throttle protein EttA / ABC-transporter extension domain / ABC transporter / Ribosomal protein L1, bacterial-type / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / : / Ribosomal protein S14, type Z ...Energy-dependent translational throttle protein EttA / ABC-transporter extension domain / ABC transporter / Ribosomal protein L1, bacterial-type / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / : / Ribosomal protein S14, type Z / Ribosomal protein L1, conserved site / Ribosomal protein L1 signature. / Ribosomal protein L1 / Ribosomal protein L1, 3-layer alpha/beta-sandwich / Ribosomal protein L1-like / Ribosomal protein L1/ribosomal biogenesis protein / Ribosomal protein L1p/L10e family / Ribosomal protein L31 type A / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / : / Ribosomal protein L16 signature 1. / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / : / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L17 signature. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L28/L24 superfamily / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / ABC transporter-like, conserved site / ABC transporters family signature. / : / Ribosomal protein L28 / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L18, bacterial-type / : / Ribosomal protein L6, bacterial-type / Ribosomal protein S14/S29 / Ribosomal protein S3, bacterial-type / Ribosomal protein S19, bacterial-type / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein S13, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L36 / Ribosomal protein S11, bacterial-type / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S4, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L20 signature. / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L35 / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal L28 family / ABC transporter / ABC transporter-like, ATP-binding domain
Similarity search - Domain/homology
Large ribosomal subunit protein uL14 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein uL13 / 30S ribosomal protein THX / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS4 ...Large ribosomal subunit protein uL14 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein uL13 / 30S ribosomal protein THX / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein bS20 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein bS18A / Small ribosomal subunit protein uS17 / Small ribosomal subunit protein bS16 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS14B / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein bL9 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein bL36 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL33B / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein bL31 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL25 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein uL1 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL15 / Energy-dependent translational throttle protein EttA
Similarity search - Component
Biological speciesMycobacterium tuberculosis H37Rv (bacteria) / Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.1 Å
AuthorsCui Z / Zhang J
Funding support United States, 2 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)P01AI095208 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)U24GM116787 United States
CitationJournal: Nat Commun / Year: 2022
Title: Interplay between an ATP-binding cassette F protein and the ribosome from Mycobacterium tuberculosis.
Authors: Zhicheng Cui / Xiaojun Li / Joonyoung Shin / Howard Gamper / Ya-Ming Hou / James C Sacchettini / Junjie Zhang /
Abstract: EttA, energy-dependent translational throttle A, is a ribosomal factor that gates ribosome entry into the translation elongation cycle. A detailed understanding of its mechanism of action is limited ...EttA, energy-dependent translational throttle A, is a ribosomal factor that gates ribosome entry into the translation elongation cycle. A detailed understanding of its mechanism of action is limited due to the lack of high-resolution structures along its ATPase cycle. Here we present the cryo-electron microscopy (cryo-EM) structures of EttA from Mycobacterium tuberculosis (Mtb), referred to as MtbEttA, in complex with the Mtb 70S ribosome initiation complex (70SIC) at the pre-hydrolysis (ADPNP) and transition (ADP-VO) states, and the crystal structure of MtbEttA alone in the post-hydrolysis (ADP) state. We observe that MtbEttA binds the E-site of the Mtb 70SIC, remodeling the P-site tRNA and the ribosomal intersubunit bridge B7a during the ribosomal ratcheting. In return, the rotation of the 30S causes conformational changes in MtbEttA, forcing the two nucleotide-binding sites (NBSs) to alternate to engage each ADPNP in the pre-hydrolysis states, followed by complete engagements of both ADP-VO molecules in the ATP-hydrolysis transition states. In the post-hydrolysis state, the conserved ATP-hydrolysis motifs of MtbEttA dissociate from both ADP molecules, leaving two nucleotide-binding domains (NBDs) in an open conformation. These structures reveal a dynamic interplay between MtbEttA and the Mtb ribosome, providing insights into the mechanism of translational regulation by EttA-like proteins.
History
DepositionMay 12, 2021-
Header (metadata) releaseFeb 2, 2022-
Map releaseFeb 2, 2022-
UpdateMay 29, 2024-
Current statusMay 29, 2024Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7msz
  • Surface level: 0.02
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_23972.png

Downloads & links

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Map

FileDownload / File: emd_23972.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.06 Å/pix.
x 320 pix.
= 340.16 Å		1.06 Å/pix.
x 320 pix.
= 340.16 Å		1.06 Å/pix.
x 320 pix.
= 340.16 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.063 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.03 / Movie #1: 0.03
Minimum - Maximum-0.005295941 - 0.3732738
Average (Standard dev.)0.0013718326 (±0.012716157)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions320320320
Spacing320320320
CellA=B=C: 340.15997 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.0631.0631.063
M x/y/z320320320
origin x/y/z0.0000.0000.000
length x/y/z340.160340.160340.160
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS320320320
D min/max/mean-0.0050.3730.001

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Supplemental data

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Sample components

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Entire : The complex of Mtb 70SIC with MtbEttA at ATP-hydrolysis transitio...

EntireName: The complex of Mtb 70SIC with MtbEttA at ATP-hydrolysis transition state (Trans_R1)
Components
  • Complex: The complex of Mtb 70SIC with MtbEttA at ATP-hydrolysis transition state (Trans_R1)
    • Protein or peptide: 50S ribosomal protein L32
    • Protein or peptide: 50S ribosomal protein L33 2
    • Protein or peptide: 50S ribosomal protein L34
    • Protein or peptide: 50S ribosomal protein L35
    • Protein or peptide: 50S ribosomal protein L36
    • Protein or peptide: 50S ribosomal protein L31
    • Protein or peptide: 50S ribosomal protein L37
    • Protein or peptide: 50S ribosomal protein L1
    • RNA: 23S rRNA
    • RNA: 5S rRNA
    • Protein or peptide: 50S ribosomal protein L2
    • Protein or peptide: 50S ribosomal protein L3
    • Protein or peptide: 50S ribosomal protein L4
    • Protein or peptide: 50S ribosomal protein L5
    • Protein or peptide: 50S ribosomal protein L6
    • Protein or peptide: 50S ribosomal protein L9
    • Protein or peptide: 50S ribosomal protein L13
    • Protein or peptide: 50S ribosomal protein L14
    • Protein or peptide: 50S ribosomal protein L15
    • Protein or peptide: 50S ribosomal protein L16
    • Protein or peptide: 50S ribosomal protein L17
    • Protein or peptide: 50S ribosomal protein L18
    • Protein or peptide: 50S ribosomal protein L19
    • Protein or peptide: 50S ribosomal protein L20
    • Protein or peptide: 50S ribosomal protein L21
    • Protein or peptide: 50S ribosomal protein L22
    • Protein or peptide: 50S ribosomal protein L23
    • Protein or peptide: 50S ribosomal protein L24
    • Protein or peptide: 50S ribosomal protein L25
    • Protein or peptide: 50S ribosomal protein L27
    • Protein or peptide: 50S ribosomal protein L28
    • Protein or peptide: 50S ribosomal protein L29
    • Protein or peptide: 50S ribosomal protein L30
    • RNA: 16S rRNA
    • Protein or peptide: 30S ribosomal protein S3
    • Protein or peptide: 30S ribosomal protein S4
    • Protein or peptide: 30S ribosomal protein S5
    • Protein or peptide: 30S ribosomal protein S6
    • Protein or peptide: 30S ribosomal protein S7
    • Protein or peptide: 30S ribosomal protein S8
    • Protein or peptide: 30S ribosomal protein S9
    • Protein or peptide: 30S ribosomal protein S10
    • Protein or peptide: 30S ribosomal protein S11
    • Protein or peptide: 30S ribosomal protein S12
    • Protein or peptide: 30S ribosomal protein S13
    • Protein or peptide: 30S ribosomal protein S14 type Z
    • Protein or peptide: 30S ribosomal protein S15
    • Protein or peptide: 30S ribosomal protein S16
    • Protein or peptide: 30S ribosomal protein S17
    • Protein or peptide: 30S ribosomal protein S18 1
    • Protein or peptide: 30S ribosomal protein S19
    • Protein or peptide: 30S ribosomal protein S20
    • Protein or peptide: Energy-dependent translational throttle protein EttA
    • RNA: initiator tRNA (Met)
    • RNA: mRNA
  • Ligand: ZINC ION
  • Ligand: MAGNESIUM ION
  • Ligand: ADENOSINE-5'-DIPHOSPHATE
  • Ligand: VANADATE ION
  • Ligand: N-FORMYLMETHIONINE

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Supramolecule #1: The complex of Mtb 70SIC with MtbEttA at ATP-hydrolysis transitio...

SupramoleculeName: The complex of Mtb 70SIC with MtbEttA at ATP-hydrolysis transition state (Trans_R1)
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#55
Source (natural)Organism: Mycobacterium tuberculosis H37Rv (bacteria)

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Macromolecule #1: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 6.525757 KDa
SequenceString:
MAVPKRRKSR SNTRSRRSQW KAAKTELVGV TVAGHAHKVP RRLLKAARLG LIDFDKR

UniProtKB: Large ribosomal subunit protein bL32

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Macromolecule #2: 50S ribosomal protein L33 2

MacromoleculeName: 50S ribosomal protein L33 2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 6.512581 KDa
SequenceString:
MASSTDVRPK ITLACEVCKH RNYITKKNRR NDPDRLELKK FCPNCGKHQA HRETR

UniProtKB: Large ribosomal subunit protein bL33B

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Macromolecule #3: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 5.643697 KDa
SequenceString:
MTKGKRTFQP NNRRRARVHG FRLRMRTRAG RSIVSSRRRK GRRTLSA

UniProtKB: Large ribosomal subunit protein bL34

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Macromolecule #4: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 7.24143 KDa
SequenceString:
MPKAKTHSGA SKRFRRTGTG KIVRQKANRR HLLEHKPSTR TRRLDGRTVV AANDTKRVTS LLNG

UniProtKB: Large ribosomal subunit protein bL35

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Macromolecule #5: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 4.323239 KDa
SequenceString:
VKVNPSVKPI CDKCRLIRRH GRVMVICSDP RHKQRQG

UniProtKB: Large ribosomal subunit protein bL36

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Macromolecule #6: 50S ribosomal protein L31

MacromoleculeName: 50S ribosomal protein L31 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 8.770074 KDa
SequenceString:
MKSDIHPAYE ETTVVCGCGN TFQTRSTKPG GRIVVEVCSQ CHPFYTGKQK ILDSGGRVAR FEKRYGKRKV GADKAVSTGK

UniProtKB: Large ribosomal subunit protein bL31

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Macromolecule #7: 50S ribosomal protein L37

MacromoleculeName: 50S ribosomal protein L37 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 2.882402 KDa
SequenceString:
MAKRGRKKRD RKYSKANHGK RPNS

UniProtKB: 30S ribosomal protein THX

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Macromolecule #8: 50S ribosomal protein L1

MacromoleculeName: 50S ribosomal protein L1 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 24.79342 KDa
SequenceString: MSKTSKAYRA AAAKVDRTNL YTPLQAAKLA KETSSTKQDA TVEVAIRLGV DPRKADQMVR GTVNLPHGTG KTARVAVFAV GEKADAAVA AGADVVGSDD LIERIQGGWL EFDAAIATPD QMAKVGRIAR VLGPRGLMPN PKTGTVTADV AKAVADIKGG K INFRVDKQ ...String:
MSKTSKAYRA AAAKVDRTNL YTPLQAAKLA KETSSTKQDA TVEVAIRLGV DPRKADQMVR GTVNLPHGTG KTARVAVFAV GEKADAAVA AGADVVGSDD LIERIQGGWL EFDAAIATPD QMAKVGRIAR VLGPRGLMPN PKTGTVTADV AKAVADIKGG K INFRVDKQ ANLHFVIGKA SFDEKLLAEN YGAAIDEVLR LKPSSSKGRY LKKITVSTTT GPGIPVDPSI TRNFAGE

UniProtKB: Large ribosomal subunit protein uL1

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Macromolecule #11: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 30.644002 KDa
SequenceString: MAIRKYKPTT PGRRGASVSD FAEITRSTPE KSLVRPLHGR GGRNAHGRIT TRHKGGGHKR AYRMIDFRRN DKDGVNAKVA HIEYDPNRT ARIALLHYLD GEKRYIIAPN GLSQGDVVES GANADIKPGN NLPLRNIPAG TLIHAVELRP GGGAKLARSA G SSIQLLGK ...String:
MAIRKYKPTT PGRRGASVSD FAEITRSTPE KSLVRPLHGR GGRNAHGRIT TRHKGGGHKR AYRMIDFRRN DKDGVNAKVA HIEYDPNRT ARIALLHYLD GEKRYIIAPN GLSQGDVVES GANADIKPGN NLPLRNIPAG TLIHAVELRP GGGAKLARSA G SSIQLLGK EASYASLRMP SGEIRRVDVR CRATVGEVGN AEQANINWGK AGRMRWKGKR PSVRGVVMNP VDHPHGGGEG KT SGGRHPV SPWGKPEGRT RNANKSSNKF IVRRRRTGKK HSR

UniProtKB: Large ribosomal subunit protein uL2

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Macromolecule #12: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 23.127533 KDa
SequenceString: MARKGILGTK LGMTQVFDES NRVVPVTVVK AGPNVVTRIR TPERDGYSAV QLAYGEISPR KVNKPLTGQY TAAGVNPRRY LAELRLDDS DAATEYQVGQ ELTAEIFADG SYVDVTGTSK GKGFAGTMKR HGFRGQGASH GAQAVHRRPG SIGGCATPAR V FKGTRMAG ...String:
MARKGILGTK LGMTQVFDES NRVVPVTVVK AGPNVVTRIR TPERDGYSAV QLAYGEISPR KVNKPLTGQY TAAGVNPRRY LAELRLDDS DAATEYQVGQ ELTAEIFADG SYVDVTGTSK GKGFAGTMKR HGFRGQGASH GAQAVHRRPG SIGGCATPAR V FKGTRMAG RMGNDRVTVL NLLVHKVDAE NGVLLIKGAV PGRTGGLVMV RSAIKRGEK

UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #13: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 23.779936 KDa
SequenceString: MAAQEQKTLK IDVKTPAGKV DGAIELPAEL FDVPANIALM HQVVTAQRAA ARQGTHSTKT RGEVSGGGRK PYRQKGTGRA RQGSTRAPQ FTGGGVVHGP KPRDYSQRTP KKMIAAALRG ALSDRARNGR IHAITELVEG QNPSTKSARA FLASLTERKQ V LVVIGRSD ...String:
MAAQEQKTLK IDVKTPAGKV DGAIELPAEL FDVPANIALM HQVVTAQRAA ARQGTHSTKT RGEVSGGGRK PYRQKGTGRA RQGSTRAPQ FTGGGVVHGP KPRDYSQRTP KKMIAAALRG ALSDRARNGR IHAITELVEG QNPSTKSARA FLASLTERKQ V LVVIGRSD EAGAKSVRNL PGVHILAPDQ LNTYDVLRAD DVVFSVEALN AYIAANTTTS EEVSA

UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #14: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 21.052303 KDa
SequenceString:
MTTAQKVQPR LKERYRSEIR DALRKQFGYG NVMQIPTVTK VVVNMGVGEA ARDAKLINGA VNDLALITGQ KPEVRRARKS IAQFKLREG MPVGVRVTLR GDRMWEFLDR LTSIALPRIR DFRGLSPKQF DGVGNYTFGL AEQAVFHEVD VDKIDRVRGM D INVVTSAA TDDEGRALLR ALGFPFKEN

UniProtKB: Large ribosomal subunit protein uL5

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Macromolecule #15: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 19.408363 KDa
SequenceString:
MSRIGKQPIP VPAGVDVTIE GQSISVKGPK GTLGLTVAEP IKVARNDDGA IVVTRPDDER RNRSLHGLSR TLVSNLVTGV TQGYTTKME IFGVGYRVQL KGSNLEFALG YSHPVVIEAP EGITFAVQAP TKFTVSGIDK QKVGQIAANI RRLRRPDPYK G KGVRYEGE QIRRKVGKTG K

UniProtKB: Large ribosomal subunit protein uL6

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Macromolecule #16: 50S ribosomal protein L9

MacromoleculeName: 50S ribosomal protein L9 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 16.196654 KDa
SequenceString:
MKLILTADVD HLGSIGDTVE VKDGYGRNFL LPRGLAIVAS RGAQKQADEI RRARETKSVR DLEHANEIKA AIEALGPIAL PVKTSADSG KLFGSVTAAD VVAAIKKAGG PNLDKRIVRL PKTHIKAVGT HFVSVHLHPE IDVEVSLDVV AQS

UniProtKB: Large ribosomal subunit protein bL9

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Macromolecule #17: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 21.646121 KDa
SequenceString: MLVGKLLGWR AVRRDLGSMS VAETNPPFTP DRRPGHPGIH PREKKVSAVP TYAPKAGDTT RSWYVIDATD VVLGRLAVAA ANLLRGKHK PTFAPNVDGG DFVIVINADK VAISGDKLQH KMVYRHSGYP GGLHKRTIGE LMQRHPDRVV EKAILGMLPK N RLSRQIQR ...String:
MLVGKLLGWR AVRRDLGSMS VAETNPPFTP DRRPGHPGIH PREKKVSAVP TYAPKAGDTT RSWYVIDATD VVLGRLAVAA ANLLRGKHK PTFAPNVDGG DFVIVINADK VAISGDKLQH KMVYRHSGYP GGLHKRTIGE LMQRHPDRVV EKAILGMLPK N RLSRQIQR KLRVYAGPEH PHSAQQPVPY ELKQVAQ

UniProtKB: Large ribosomal subunit protein uL13

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Macromolecule #18: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 13.419649 KDa
SequenceString:
VIQQESRLKV ADNTGAKEIL CIRVLGGSSR RYAGIGDVIV ATVKDAIPGG NVKRGDVVKA VVVRTVKERR RPDGSYIKFD ENAAVIIKP DNDPRGTRIF GPVGRELREK RFMKIISLAP EVL

UniProtKB: Large ribosomal subunit protein uL14

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Macromolecule #19: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 15.566126 KDa
SequenceString:
MTLKLHDLRP ARGSKIARTR VGRGDGSKGK TAGRGTKGTR ARKQVPVTFE GGQMPIHMRL PKLKGFRNRF RTEYEIVNVG DINRLFPQG GAVGVDDLVA KGAVRKNALV KVLGDGKLTA KVDVSAHKFS GSARAKITAA GGSATEL

UniProtKB: Large ribosomal subunit protein uL15

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Macromolecule #20: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 15.724277 KDa
SequenceString:
MLIPRKVKHR KQHHPRQRGI ASGGTTVNFG DYGIQALEHA YVTNRQIESA RIAINRHIKR GGKVWINIFP DRPLTKKPAE TRMGSGKGS PEWWVANVKP GRVLFELSYP NEGVARAALT RAIHKLPIKA RIITREEQF

UniProtKB: Large ribosomal subunit protein uL16

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Macromolecule #21: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 19.512273 KDa
SequenceString:
MPKPTKGPRL GGSSSHQKAI LANLATSLFE HGRITTTEPK ARALRPYAEK LITHAKKGAL HNRREVLKKL RDKDVVHTLF AEIGPFFAD RDGGYTRIIK IEARKGDNAP MAVIELVREK TVTSEANRAR RVAAAQAKAK KAAAMPTEES EAKPAEEGDV V GASEPDAK APEEPPAEAP EN

UniProtKB: Large ribosomal subunit protein bL17

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Macromolecule #22: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 13.211122 KDa
SequenceString:
MAQSVSATRR ISRLRRHTRL RKKLSGTAER PRLVVHRSAR HIHVQLVNDL NGTTVAAASS IEADVRGVPG DKKARSVRVG QLIAERAKA AGIDTVVFDR GGYTYGGRIA ALADAARENG LSF

UniProtKB: Large ribosomal subunit protein uL18

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Macromolecule #23: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 13.041126 KDa
SequenceString:
MNRLDFVDKP SLRDDIPAFN PGDTINVHVK VIEGAKERLQ VFKGVVIRRQ GGGIRETFTV RKESYGVGVE RTFPVHSPNI DHIEVVTRG DVRRAKLYYL RELRGKKAKI KEKR

UniProtKB: Large ribosomal subunit protein bL19

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Macromolecule #24: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 14.557777 KDa
SequenceString:
MARVKRAVNA HKKRRSILKA SRGYRGQRSR LYRKAKEQQL HSLNYAYRDR RARKGEFRKL WIARINAAAR LNDITYNRLI QGLKAAGVE VDRKNLADIA ISDPAAFTAL VDVARAALPE DVNAPSGEAA

UniProtKB: Large ribosomal subunit protein bL20

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Macromolecule #25: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 11.173147 KDa
SequenceString:
MMATYAIVKT GGKQYKVAVG DVVKVEKLES EQGEKVSLPV ALVVDGATVT TDAKALAKVA VTGEVLGHTK GPKIRIHKFK NKTGYHKRQ GHRQQLTVLK VTGIA

UniProtKB: Large ribosomal subunit protein bL21

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Macromolecule #26: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 20.424594 KDa
SequenceString: MTAATKATEY PSAVAKARFV RVSPRKARRV IDLVRGRSVS DALDILRWAP QAASGPVAKV IASAAANAQN NGGLDPATLV VATVYADQG PTAKRIRPRA QGRAFRIRRR TSHITVVVES RPAKDQRSAK SSRARRTEAS KAASKVGATA PAKKAAAKAP A KKAPASSG ...String:
MTAATKATEY PSAVAKARFV RVSPRKARRV IDLVRGRSVS DALDILRWAP QAASGPVAKV IASAAANAQN NGGLDPATLV VATVYADQG PTAKRIRPRA QGRAFRIRRR TSHITVVVES RPAKDQRSAK SSRARRTEAS KAASKVGATA PAKKAAAKAP A KKAPASSG VKKTPAKKAP AKKAPAKASE TSAAKGGSD

UniProtKB: Large ribosomal subunit protein uL22

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Macromolecule #27: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 10.976785 KDa
SequenceString:
MATLADPRDI ILAPVISEKS YGLLDDNVYT FLVRPDSNKT QIKIAVEKIF AVKVASVNTA NRQGKRKRTR TGYGKRKSTK RAIVTLAPG SRPIDLFGAP A

UniProtKB: Large ribosomal subunit protein uL23

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Macromolecule #28: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 11.500274 KDa
SequenceString:
MKVHKGDTVL VISGKDKGAK GKVLQAYPDR NRVLVEGVNR IKKHTAISTT QRGARSGGIV TQEAPIHVSN VMVVDSDGKP TRIGYRVDE ETGKRVRISK RNGKDI

UniProtKB: Large ribosomal subunit protein uL24

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Macromolecule #29: 50S ribosomal protein L25

MacromoleculeName: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 22.466166 KDa
SequenceString: MAKSASNQLR VTVRTETGKG ASRRARRAGK IPAVLYGHGA EPQHLELPGH DYAAVLRHSG TNAVLTLDIA GKEQLALTKA LHIHPIRRT IQHADLLVVR RGEKVVVEVS VVVEGQAGPD TLVTQETNSI EIEAEALSIP EQLTVSIEGA EPGTQLTAGQ I ALPAGVSL ...String:
MAKSASNQLR VTVRTETGKG ASRRARRAGK IPAVLYGHGA EPQHLELPGH DYAAVLRHSG TNAVLTLDIA GKEQLALTKA LHIHPIRRT IQHADLLVVR RGEKVVVEVS VVVEGQAGPD TLVTQETNSI EIEAEALSIP EQLTVSIEGA EPGTQLTAGQ I ALPAGVSL ISDPDLLVVN VVKAPTAEEL EGEVAGAEEA EEAAVEAGEA EAAGESE

UniProtKB: Large ribosomal subunit protein bL25

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Macromolecule #30: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 8.987272 KDa
SequenceString:
MAHKKGASSS RNGRDSAAQR LGVKRYGGQV VKAGEILVRQ RGTKFHPGVN VGRGGDDTLF AKTAGAVEFG IKRGRKTVSI VGSTTA

UniProtKB: Large ribosomal subunit protein bL27

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Macromolecule #31: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 6.920075 KDa
SequenceString:
MAAVCDICGK GPGFGKSVSH SHRRTSRRWD PNIQTVHAVT RPGGNKKRLN VCTSCIKAGK ITRG

UniProtKB: Large ribosomal subunit protein bL28

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Macromolecule #32: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 8.872015 KDa
SequenceString:
MAVGVSPGEL RELTDEELAE RLRESKEELF NLRFQMATGQ LNNNRRLRTV RQEIARIYTV LRERELGLAT GPDGKES

UniProtKB: Large ribosomal subunit protein uL29

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Macromolecule #33: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 7.361569 KDa
SequenceString:
MSQLKITQVR STIGARWKQR ESLRTLGLRR IRHSVIREDN AATRGLIAVV RHLVEVEPAQ TGGKT

UniProtKB: Large ribosomal subunit protein uL30

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Macromolecule #35: 30S ribosomal protein S3

MacromoleculeName: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 30.071018 KDa
SequenceString: MGQKINPHGF RLGITTDWKS RWYADKQYAE YVKEDVAIRR LLSSGLERAG IADVEIERTR DRVRVDIHTA RPGIVIGRRG TEADRIRAD LEKLTGKQVQ LNILEVKNPE SQAQLVAQGV AEQLSNRVAF RRAMRKAIQS AMRQPNVKGI RVQCSGRLGG A EMSRSEFY ...String:
MGQKINPHGF RLGITTDWKS RWYADKQYAE YVKEDVAIRR LLSSGLERAG IADVEIERTR DRVRVDIHTA RPGIVIGRRG TEADRIRAD LEKLTGKQVQ LNILEVKNPE SQAQLVAQGV AEQLSNRVAF RRAMRKAIQS AMRQPNVKGI RVQCSGRLGG A EMSRSEFY REGRVPLHTL RADIDYGLYE AKTTFGRIGV KVWIYKGDIV GGKRELAAAA PAGADRPRRE RPSGTRPRRS GA SGTTATG TDAGRAAGGE EAAPDAAAPV EAQSTES

UniProtKB: Small ribosomal subunit protein uS3

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Macromolecule #36: 30S ribosomal protein S4

MacromoleculeName: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 23.515898 KDa
SequenceString: MARYTGPVTR KSRRLRTDLV GGDQAFEKRP YPPGQHGRAR IKESEYLLQL QEKQKARFTY GVMEKQFRRY YEEAVRQPGK TGEELLKIL ESRLDNVIYR AGLARTRRMA RQLVSHGHFN VNGVHVNVPS YRVSQYDIVD VRDKSLNTVP FQIARETAGE R PIPSWLQV ...String:
MARYTGPVTR KSRRLRTDLV GGDQAFEKRP YPPGQHGRAR IKESEYLLQL QEKQKARFTY GVMEKQFRRY YEEAVRQPGK TGEELLKIL ESRLDNVIYR AGLARTRRMA RQLVSHGHFN VNGVHVNVPS YRVSQYDIVD VRDKSLNTVP FQIARETAGE R PIPSWLQV VGERQRVLIH QLPERAQIDV PLTEQLIVEY YSK

UniProtKB: Small ribosomal subunit protein uS4

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Macromolecule #37: 30S ribosomal protein S5

MacromoleculeName: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 22.925242 KDa
SequenceString: MAEQPAGQAG TTDNRDARGD REGRRRDSGR GSRERDGEKS NYLERVVAIN RVSKVVKGGR RFSFTALVIV GDGNGMVGVG YGKAKEVPA AIAKGVEEAR KSFFRVPLIG GTITHPVQGE AAAGVVLLRP ASPGTGVIAG GAARAVLECA GVHDILAKSL G SDNAINVV ...String:
MAEQPAGQAG TTDNRDARGD REGRRRDSGR GSRERDGEKS NYLERVVAIN RVSKVVKGGR RFSFTALVIV GDGNGMVGVG YGKAKEVPA AIAKGVEEAR KSFFRVPLIG GTITHPVQGE AAAGVVLLRP ASPGTGVIAG GAARAVLECA GVHDILAKSL G SDNAINVV HATVAALKLL QRPEEVAARR GLPIEDVAPA GMLKARRKSE ALAASVLPDR TI

UniProtKB: Small ribosomal subunit protein uS5

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Macromolecule #38: 30S ribosomal protein S6

MacromoleculeName: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 10.952728 KDa
SequenceString:
MRPYEIMVIL DPTLDERTVA PSLETFLNVV RKDGGKVEKV DIWGKRRLAY EIAKHAEGIY VVIDVKAAPA TVSELDRQLS LNESVLRTK VMRTDKH

UniProtKB: Small ribosomal subunit protein bS6

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Macromolecule #39: 30S ribosomal protein S7

MacromoleculeName: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 17.633418 KDa
SequenceString:
MPRKGPAPKR PLVNDPVYGS QLVTQLVNKV LLKGKKSLAE RIVYGALEQA RDKTGTDPVI TLKRALDNVK PALEVRSRRV GGATYQVPV EVRPDRSTTL ALRWLVGYSR QRREKTMIER LANEILDASN GLGASVKRRE DTHKMAEANR AFAHYRW

UniProtKB: Small ribosomal subunit protein uS7

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Macromolecule #40: 30S ribosomal protein S8

MacromoleculeName: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 14.432563 KDa
SequenceString:
MTMTDPIADF LTRLRNANSA YHDEVSLPHS KLKANIAQIL KNEGYISDFR TEDARVGKSL VIQLKYGPSR ERSIAGLRRV SKPGLRVYA KSTNLPRVLG GLGVAIISTS SGLLTDRQAA RQGVGGEVLA YVW

UniProtKB: Small ribosomal subunit protein uS8

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Macromolecule #41: 30S ribosomal protein S9

MacromoleculeName: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 16.465061 KDa
SequenceString:
MTETTPAPQT PAAPAGPAQS FVLERPIQTV GRRKEAVVRV RLVPGTGKFD LNGRSLEDYF PNKVHQQLIK APLVTVDRVE SFDIFAHLG GGGPSGQAGA LRLGIARALI LVSPEDRPAL KKAGFLTRDP RATERKKYGL KKARKAPQYS KR

UniProtKB: Small ribosomal subunit protein uS9

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Macromolecule #42: 30S ribosomal protein S10

MacromoleculeName: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 11.45234 KDa
SequenceString:
MAGQKIRIRL KAYDHEAIDA SARKIVETVV RTGASVVGPV PLPTEKNVYC VIRSPHKYKD SREHFEMRTH KRLIDIIDPT PKTVDALMR IDLPASVDVN IQ

UniProtKB: Small ribosomal subunit protein uS10

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Macromolecule #43: 30S ribosomal protein S11

MacromoleculeName: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 14.815918 KDa
SequenceString:
MPPAKKGPAT SARKGQKTRR REKKNVPHGA AHIKSTFNNT IVTITDPQGN VIAWASSGHV GFKGSRKSTP FAAQLAAENA ARKAQDHGV RKVDVFVKGP GSGRETAIRS LQAAGLEVGA ISDVTPQPHN GVRPPKRRRV

UniProtKB: Small ribosomal subunit protein uS11

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Macromolecule #44: 30S ribosomal protein S12

MacromoleculeName: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 13.882341 KDa
SequenceString:
MPTIQQLVRK GRRDKISKVK TAALKGSPQR RGVCTRVYTT TPKKPNSALR KVARVKLTSQ VEVTAYIPGE GHNLQEHSMV LVRGGRVKD LPGVRYKIIR GSLDTQGVKN RKQARSRYGA KKEKG

UniProtKB: Small ribosomal subunit protein uS12

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Macromolecule #45: 30S ribosomal protein S13

MacromoleculeName: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 14.383779 KDa
SequenceString:
MARLVGVDLP RDKRMEVALT YIFGIGRTRS NEILAATGID RDLRTRDLTE EQLIHLRDYI EANLKVEGDL RREVQADIRR KIEIGCYQG LRHRRGMPVR GQRTKTNART RKGPKRTIAG KKKAR

UniProtKB: Small ribosomal subunit protein uS13

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Macromolecule #46: 30S ribosomal protein S14 type Z

MacromoleculeName: 30S ribosomal protein S14 type Z / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 6.841249 KDa
SequenceString:
MAKKALVNKA AGKPRFAVRA YTRCSKCGRP RAVYRKFGLC RICLREMAHA GELPGVQKSS W

UniProtKB: Small ribosomal subunit protein uS14B

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Macromolecule #47: 30S ribosomal protein S15

MacromoleculeName: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 10.500277 KDa
SequenceString:
MALTAEQKKE ILRSYGLHET DTGSPEAQIA LLTKRIADLT EHLKVHKHDH HSRRGLLLLV GRRRRLIKYI SQIDVERYRS LIERLGLRR

UniProtKB: Small ribosomal subunit protein uS15

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Macromolecule #48: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 17.4661 KDa
SequenceString:
MAVKIKLTRL GKIRNPQYRV AVADARTRRD GRAIEVIGRY HPKEEPSLIE INSERAQYWL SVGAQPTEPV LKLLKITGDW QKFKGLPGA QGRLKVAAPK PSKLEVFNAA LAAADGGPTT EATKPKKKSP AKKAAKAAEP APQPEQPDTP ALGGEQAELT A ES

UniProtKB: Small ribosomal subunit protein bS16

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Macromolecule #49: 30S ribosomal protein S17

MacromoleculeName: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 14.776373 KDa
SequenceString:
MAEAKTGAKA APRVAKAAKA APKKAAPNDA EAIGAANAAN VKGPKHTPRT PKPRGRRKTR IGYVVSDKMQ KTIVVELEDR MRHPLYGKI IRTTKKVKAH DEDSVAGIGD RVSLMETRPL SATKRWRLVE ILEKAK

UniProtKB: Small ribosomal subunit protein uS17

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Macromolecule #50: 30S ribosomal protein S18 1

MacromoleculeName: 30S ribosomal protein S18 1 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 9.565279 KDa
SequenceString:
MAKSSKRRPA PEKPVKTRKC VFCAKKDQAI DYKDTALLRT YISERGKIRA RRVTGNCVQH QRDIALAVKN AREVALLPFT SSVR

UniProtKB: Small ribosomal subunit protein bS18A

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Macromolecule #51: 30S ribosomal protein S19

MacromoleculeName: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 10.830629 KDa
SequenceString:
MPRSLKKGPF VDEHLLKKVD VQNEKNTKQV IKTWSRRSTI IPDFIGHTFA VHDGRKHVPV FVTESMVGHK LGEFAPTRTF KGHIKDDRK SKRR

UniProtKB: Small ribosomal subunit protein uS19

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Macromolecule #52: 30S ribosomal protein S20

MacromoleculeName: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 9.428941 KDa
SequenceString:
MANIKSQQKR NRTNERARLR NKAVKSSLRT AVRAFREAAH AGDKAKAAEL LASTNRKLDK AASKGVIHKN QAANKKSALA QALNKL

UniProtKB: Small ribosomal subunit protein bS20

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Macromolecule #53: Energy-dependent translational throttle protein EttA

MacromoleculeName: Energy-dependent translational throttle protein EttA / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
EC number: Hydrolases; Acting on acid anhydrides; In phosphorus-containing anhydrides
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 61.972957 KDa
SequenceString: MAEFIYTMKK VRKAHGDKVI LDDVTLSFYP GAKIGVVGPN GAGKSSVLRI MAGLDKPNNG DAFLATGATV GILQQEPPLN EDKTVRGNV EEGMGDIKIK LDRFNEVAEL MATDYTDELM EEMGRLQEEL DHADAWDLDA QLEQAMDALR CPPADEPVTN L SGGERRRV ...String:
MAEFIYTMKK VRKAHGDKVI LDDVTLSFYP GAKIGVVGPN GAGKSSVLRI MAGLDKPNNG DAFLATGATV GILQQEPPLN EDKTVRGNV EEGMGDIKIK LDRFNEVAEL MATDYTDELM EEMGRLQEEL DHADAWDLDA QLEQAMDALR CPPADEPVTN L SGGERRRV ALCKLLLSKP DLLLLDEPTN HLDAESVQWL EQHLASYPGA ILAVTHDRYF LDNVAEWILE LDRGRAYPYE GN YSTYLEK KAERLAVQGR KDAKLQKRLT EELAWVRSGA KARQAKSKAR LQRYEEMAAE AEKTRKLDFE EIQIPVGPRL GNV VVEVDH LDKGYDGRAL IKDLSFSLPR NGIVGVIGPN GVGKTTLFKT IVGLETPDSG SVKVGETVKL SYVDQARAGI DPRK TVWEV VSDGLDYIQV GQTEVPSRAY VSAFGFKGPD QQKPAGVLSG GERNRLNLAL TLKQGGNLIL LDEPTNDLDV ETLGS LENA LLNFPGCAVV ISHDRWFLDR TCTHILAWEG DDDNEAKWFW FEGNFGAYEE NKVERLGVDA ARPHRVTHRK LTRG

UniProtKB: Energy-dependent translational throttle protein EttA

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Macromolecule #9: 23S rRNA

MacromoleculeName: 23S rRNA / type: rna / ID: 9 / Number of copies: 1
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 1.017820562 MDa
SequenceString: UUGUAAGUGU CUAAGGGCGC AUGGUGGAUG CCUUGGCAUC GAGAGCCGAU GAAGGACGUG GGAGGCUGCG AUAUGCCUCG GGGAGCUGU CAACCGAGCG UGGAUCCGAG GAUUUCCGAA UGGGGAAACC CAGCACGAGU GAUGUCGUGC UACCCGCAUC U GAAUAUAU ...String:
UUGUAAGUGU CUAAGGGCGC AUGGUGGAUG CCUUGGCAUC GAGAGCCGAU GAAGGACGUG GGAGGCUGCG AUAUGCCUCG GGGAGCUGU CAACCGAGCG UGGAUCCGAG GAUUUCCGAA UGGGGAAACC CAGCACGAGU GAUGUCGUGC UACCCGCAUC U GAAUAUAU AGGGUGCGGG AGGGAACGCG GGGAAGUGAA ACAUCUCAGU ACCCGUAGGA GGAGAAAACA AUUGUGAUUC CG CAAGUAG UGGCGAGCGA ACGCGGAACA GGCUAAACCG CACGCAUGGG UAACCGGGUA GGGGUUGUGU GUGCGGGGUU GUG GGAGGA UAUGUCUCAG CGCUACCCGG CUGAGAGGCA GUCAGAAAGU GUCGUGGUUA GCGGAAGUGG CCUGGGAUGG UCUG CCGUA GACGGUGAGA GCCCGGUACG CGAAAACCCG GCACCUGCCU AGUAUCAAUU CCCGAGUAGC AGCGGGCCCG UGGAA UCCG CUGUGAAUCC GCCGGGACCA CCCGGUAAGC CUAAAUACUC CUCGAUGACC GAUAGCGGAU UAGUACCGUG AGGGAA UGG UGAAAAGUAC CCCGGGAGGG GAGUGAAAGA GUACCUGAAA CCGUGUGCCU ACAAUCCGUC AGAGCCUCCU UUUCCUC UC CGGAGGAGGG UGGUGAUGGC GUGCCUUUUG AAGAAUGAGC CUGCGAGUCA GGGACAUGUC GCAAGGUUAA CCCGUGUG G GGUAGCCGCA GCGAAAGCGA GUCUGAAUAG GGCGACCCAC ACGCGCAUAC GCGCGUGUGA AUAGUGGCGU GUUCUGGAC CCGAAGCGGA GUGAUCUACC CAUGGCCAGG GUGAAGCGCG GGUAAGACCG CGUGGAGGCC CGAACCCACU UAGGUUGAAG ACUGAGGGG AUGAGCUGUG GGUAGGGGUG AAAGGCCAAU CAAACUCCGU GAUAGCUGGU UCUCCCCGAA AUGCAUUUAG G UGCAGCGU UGCGUGGUUC ACCGCGGAGG UAGAGCUACU GGAUGGCCGA UGGGCCCUAC UAGGUUACUG ACGUCAGCCA AA CUCCGAA UGCCGUGGUG UAAAGCGUGG CAGUGAGACG GCGGGGGAUA AGCUCCGUAC GUCGAAAGGG AAACAGCCCA GAU CGCCGG CUAAGGCCCC CAAGCGUGUG CUAAGUGGGA AAGGAUGUGC AGUCGCAAAG ACAACCAGGA GGUUGGCUUA GAAG CAGCC ACCCUUGAAA GAGUGCGUAA UAGCUCACUG GUCAAGUGAU UGUGCGCCGA UAAUGUAGCG GGGCUCAAGC ACACC GCCG AAGCCGCGGC ACAUCCACCU UGUGGUGGGU GUGGGUAGGG GAGCGUCCCU CAUUCAGCGA AGCCACCGGG UGACCG GUG GUGGAGGGUG GGGGAGUGAG AAUGCAGGCA UGAGUAGCGA CAAGGCAAGU GAGAACCUUG CCCGCCGAAA GACCAAG GG UUCCUGGGCC AGGCCAGUCC GCCCAGGGUG AGUCGGGACC UAAGGCGAGG CCGACAGGCG UAGUCGAUGG ACAACGGG U UGAUAUUCCC GUACCCGUGU GUGGGCGCCC GUGACGAAUC AGCGGUACUA ACCACCCAAA ACCGGAUCGA UCACUCCCC UUCGGGGGUG UGGAGUUCUG GGGCUGCGUG GGAACUUCGC UGGUAGUAGU CAAGCGAAGG GGUGACGCAG GAAGGUAGCC GUACCAGUC AGUGGUAACA CUGGGGCAAG CCGGUAGGGA GAGCGAUAGG CAAAUCCGUC GCUCACUAAU CCUGAGAGGU G ACGCAUAG CCGGUUGAGG CGAAUUCGGU GAUCCUCUGC UGCCAAGAAA AGCCUCUAGC GAGCACACAC ACGGCCCGUA CC CCAAACC GACACAGGUG GUCAGGUAGA GCAUACCAAG GCGUACGAGA UAACUAUGGU UAAGGAACUC GGCAAAAUGC CCC CGUAAC UUCGGGAGAA GGGGGACCGG AAUAUCGUGA ACACCCUUGC GGUGGGAGCG GGAUCCGGUC GCAGAAACCA GUGA GGAGC GACUGUUUAC UAAAAACACA GGUCCGUGCG AAGUCGCAAG ACGAUGUAUA CGGACUGACG CCUGCCCGGU GCUGG AAGG UUAAGAGGAC CCGUUAACCC GCAAGGGUGA AGCGGAGAAU UUAAGCCCCA GUAAACGGCG GUGGUAACUA UAACCA UCC UAAGGUAGCG AAA(5MU)UCCUUG UCGGGUAAGU UCCGACCUGC ACGAAUGGCG UAACGACUUC UCAACUGUCU CAA CCAUAG ACUCGGCGAA AUUGCACUAC GAGUAAAGAU GCUCGUUACG CGCGGCAGGA CGAAAAGACC CCGGGACCUU CACU ACAAC UUGGUAUUGA UGUUCGGUAC GGUUUGUGUA GGAUAGGUGG GAGACUGUGA AACCUCGACG CCAGUUGGGG CGGAG UCGU UGUUGAAAUA CCACUCUGAU CGUAUUGGGC AUCUAACCUC GAACCCUGAA UCGGGUUUAG GGACAGUGCC UGGCGG GUA GUUUAACUGG GGCGGUUGCC UCCUAAAAUG UAACGGAGGC GCCCAAAGGU UCCCUCAACC UGGACGGCAA UCAGGUG GC GAGUGUAAAU GCACAAGGGA GCUUGACUGC GAGACUUACA AGUCAAGCAG GGACGAAAGU CGGGAUUAGU GAUCCGGC A CCCCCGAGUG GAAGGGGUGU CGCUCAACGG AUAAAAGGUA CCCCGGGGAU AACAGGCUGA UCUUCCCCAA GAGUCCAUA UCGACGGGAU GGUUUGGCAC CUCGAUGUCG GCUCGUCGCA UCCUGGGGCU GGAGCAGGUC CCAAGGGUUG GGCU(OMG)U UCG CCCAUUAAAG CGGCACGCGA GCUGGGUUUA GAACGUCGUG AGACAGUUCG GUCUCUAUCC GCCGCGCGCG UCAGAAA CU UGAGGAAACC UGUCCCUAGU ACGAGAGGAC CGGGACGGAC GAACCUCUGG UGCACCAGUU GUCCCGCCAG GGGCACCG C UGGAUAGCCA CGUUCGGUCA GGAUAACCGC UGAAAGCAUC UAAGCGGGAA ACCUUCUCCA AGAUCAGGUU UCUCACCCA CUUGGUGGGA UAAGGCCCCC CGCAGAACAC GGGUUCAAUA GGUCAGACCU GGAAGCUCAG UAAUGGGUGU AGGGAACUGG UGCUAACCG GCCGAAAACU UACAACA

GENBANK: GENBANK: CP023578.1

+
Macromolecule #10: 5S rRNA

MacromoleculeName: 5S rRNA / type: rna / ID: 10 / Number of copies: 1
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 37.097238 KDa
SequenceString:
UUACGGCGGC CACAGCGGCA GGGAAACGCC CGGUCCCAUU CCGAACCCGG AAGCUAAGCC UGCCAGCGCC GAUGAUACUG CCCCUCCGG GUGGAAAAGU AGGACACCGC CGAACA

GENBANK: GENBANK: CP023593.1

+
Macromolecule #34: 16S rRNA

MacromoleculeName: 16S rRNA / type: rna / ID: 34 / Number of copies: 1
Source (natural)Organism: Mycobacterium tuberculosis H37Rv (bacteria) / Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 498.40175 KDa
SequenceString: UUUUGUUUGG AGAGUUUGAU CCUGGCUCAG GACGAACGCU GGCGGCGUGC UUAACACAUG CAAGUCGAAC GGAAAGGUCU CUUCGGAGA UACUCGAGUG GCGAACGGGU GAGUAACACG UGGGUGAUCU GCCCUGCACU UCGGGAUAAG CCUGGGAAAC U GGGUCUAA ...String:
UUUUGUUUGG AGAGUUUGAU CCUGGCUCAG GACGAACGCU GGCGGCGUGC UUAACACAUG CAAGUCGAAC GGAAAGGUCU CUUCGGAGA UACUCGAGUG GCGAACGGGU GAGUAACACG UGGGUGAUCU GCCCUGCACU UCGGGAUAAG CCUGGGAAAC U GGGUCUAA UACCGGAUAG GACCACGGGA UGCAUGUCUU GUGGUGGAAA GCGCUUUAGC GGUGUGGGAU GAGCCCGCGG CC UAUCAGC UUGUUGGUGG GGUGACGGCC UACCAAGGCG ACGACGGGUA GCCGGCCUGA GAGGGUGUCC GGCCACACUG GGA CUGAGA UACGGCCCAG ACUCCUACGG GAGGCAGCAG UGGGGAAUAU UGCACAAUGG GCGCAAGCCU GAUGCAGCGA CGCC GCGUG GGGGAUGACG GCCUUCGGGU UGUAAACCUC UUUCACCAUC GACGAAGGUC CGGGUUCUCU CGGAUUGACG GUAGG UGGA GAAGAAGCAC CGGCCAACUA CGUGCCAGCA GCC(G7M)CGGUAA UACGUAGGGU GCGAGCGUUG UCCGGAAUUA CU GGGCGUA AAGAGCUCGU AGGUGGUUUG UCGCGUUGUU CGUGAAAUCU CACGGCUUAA CUGUGAGCGU GCGGGCGAUA CGG GCAGAC UAGAGUACUG CAGGGGAGAC UGGAAUUCCU GGUGUAGCGG UGGAAUGCGC AGAUAUCAGG AGGAACACCG GUGG CGAAG GCGGGUCUCU GGGCAGUAAC UGACGCUGAG GAGCGAAAGC GUGGGGAGCG AACAGGAUUA GAUACCCUGG UAGUC CACG CCGUAAACGG UGGGUACUAG GUGUGGGUUU CCUUCCUUGG GAUCCGUGCC GUAGCUAACG CAUUAAGUAC CCCGCC UGG GGAGUACGGC CGCAAGGCUA AAACUCAAAG GAAUUGACGG GGGCCCGCAC AAGCGGCGGA GCAUGUGGAU UAAUUCG AU (2MG)(5MC)AACGCGAA GAACCUUACC UGGGUUUGAC AUGCACAGGA CGCGUCUAGA GAUAGGCGUU CCCUUGUGGC CUGUGUGCA GGUGGUGCAU GGCUGUCGUC AGCUCGUGUC GUGAGAUGUU GGGUUAAGUC CCGCAACGAG CGCAACCCUU G UCUCAUGU UGCCAGCACG UAAUGGUGGG GACUCGUGAG AGACUGCCGG GGUCAACUCG GAGGAAGGUG GGGAUGACGU CA AGUCAUC AUGCCCCUUA UGUCCAGGGC UUCACACAUG CUACAAUGGC CGGUACAAAG GGCUGCGAUG CCGCGAGGUU AAG CGAAUC CUUAAAAGCC GGUCUCAGUU CGGAUCGGGG UCUGCAACUC GACCCCGUGA AGUCGGAGUC GCUAGUAAUC GCAG AUCAG CAACGCUGCG GUGAAUACGU UCCCGGGCCU UGUACACACC G(4OC)CCGUCACG UCAUGAAAGU CGGUAACACC C GAAGCCAG UGGCCUAACC CUCGGGAGGG AGCUGUCGAA GGUGGGAUCG GCGAUUGGGA CGAAGUCG(UR3)A ACAAGGUA G CCGUACCGG(MA6) (MA6)GGUGCGGCU GGAUCACCUC CUUUCU

GENBANK: GENBANK: MH794239.1

+
Macromolecule #54: initiator tRNA (Met)

MacromoleculeName: initiator tRNA (Met) / type: rna / ID: 54 / Number of copies: 1
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 24.802785 KDa
SequenceString:
CGCGGGGUGG AGCAGCCUGG UAGCUCGUCG GGCUCAUAAC CCGAAGGUCG UCGGUUCAAA UCCGGCCCCC GCAACCA

+
Macromolecule #55: mRNA

MacromoleculeName: mRNA / type: rna / ID: 55 / Number of copies: 1
Source (natural)Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv
Molecular weightTheoretical: 8.470186 KDa
SequenceString:
AGAAAGGAGG UAAAACAUGU UCAAAA

+
Macromolecule #56: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 56 / Number of copies: 6 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

+
Macromolecule #57: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 57 / Number of copies: 417 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #58: ADENOSINE-5'-DIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 58 / Number of copies: 2 / Formula: ADP
Molecular weightTheoretical: 427.201 Da
Chemical component information

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

+
Macromolecule #59: VANADATE ION

MacromoleculeName: VANADATE ION / type: ligand / ID: 59 / Number of copies: 2 / Formula: VO4
Molecular weightTheoretical: 114.939 Da
Chemical component information

ChemComp-VN3:
VANADATE ION

+
Macromolecule #60: N-FORMYLMETHIONINE

MacromoleculeName: N-FORMYLMETHIONINE / type: ligand / ID: 60 / Number of copies: 1 / Formula: FME
Molecular weightTheoretical: 177.221 Da
Chemical component information

ChemComp-FME:
N-FORMYLMETHIONINE

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.5
GridModel: Quantifoil R2/1 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 2
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 295 K

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 48.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

Particle selectionNumber selected: 8666
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

5v93

Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 32731
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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