- EMDB-23818: Cryo-EM 3D map of the S. cerevisiae origin recognition complex bo... -
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Basic information
Entry
Database: EMDB / ID: EMD-23818
Title
Cryo-EM 3D map of the S. cerevisiae origin recognition complex bound to Cdc6 and ARS1 origin DNA
Map data
Sample
Complex: Complex of origin replication complex with Cdc6 and ARS1 origin DNA
Protein or peptide: Origin recognition complex subunit 1
Protein or peptide: Origin recognition complex subunit 2
Protein or peptide: Origin recognition complex subunit 3
Protein or peptide: Origin recognition complex subunit 4
Protein or peptide: Origin recognition complex subunit 5
Protein or peptide: Origin recognition complex subunit 6
Protein or peptide: Cell division control protein 6
DNA: ARS1 DNA(85-mer)
DNA: ARS1 DNA(85-mer)
Function / homology
Function and homology information
MCM complex loading / CDC6 association with the ORC:origin complex / Cul8-RING ubiquitin ligase complex / maintenance of rDNA / Assembly of the ORC complex at the origin of replication / nuclear origin of replication recognition complex / pre-replicative complex assembly involved in nuclear cell cycle DNA replication / Activation of the pre-replicative complex / nucleosome organization / nuclear pre-replicative complex ...MCM complex loading / CDC6 association with the ORC:origin complex / Cul8-RING ubiquitin ligase complex / maintenance of rDNA / Assembly of the ORC complex at the origin of replication / nuclear origin of replication recognition complex / pre-replicative complex assembly involved in nuclear cell cycle DNA replication / Activation of the pre-replicative complex / nucleosome organization / nuclear pre-replicative complex / Activation of ATR in response to replication stress / DNA replication preinitiation complex / cyclin-dependent protein serine/threonine kinase inhibitor activity / mitotic DNA replication checkpoint signaling / silent mating-type cassette heterochromatin formation / regulation of DNA-templated DNA replication initiation / CDK-mediated phosphorylation and removal of Cdc6 / Orc1 removal from chromatin / regulation of DNA replication / DNA replication origin binding / DNA replication initiation / subtelomeric heterochromatin formation / nucleosome binding / G1/S transition of mitotic cell cycle / chromosome / chromosome, telomeric region / cell division / chromatin binding / GTPase activity / GTP binding / ATP hydrolysis activity / nucleoplasm / ATP binding / nucleus / metal ion binding / cytoplasm Similarity search - Function
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM131754
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM45436
United States
Biotechnology and Biological Sciences Research Council (BBSRC)
BB/S001387.1
United States
Biotechnology and Biological Sciences Research Council (BBSRC)
BB/N000323/1
United States
Citation
Journal: Nat Commun / Year: 2021 Title: The structure of ORC-Cdc6 on an origin DNA reveals the mechanism of ORC activation by the replication initiator Cdc6. Authors: Xiang Feng / Yasunori Noguchi / Marta Barbon / Bruce Stillman / Christian Speck / Huilin Li / Abstract: The Origin Recognition Complex (ORC) binds to sites in chromosomes to specify the location of origins of DNA replication. The S. cerevisiae ORC binds to specific DNA sequences throughout the cell ...The Origin Recognition Complex (ORC) binds to sites in chromosomes to specify the location of origins of DNA replication. The S. cerevisiae ORC binds to specific DNA sequences throughout the cell cycle but becomes active only when it binds to the replication initiator Cdc6. It has been unclear at the molecular level how Cdc6 activates ORC, converting it to an active recruiter of the Mcm2-7 hexamer, the core of the replicative helicase. Here we report the cryo-EM structure at 3.3 Å resolution of the yeast ORC-Cdc6 bound to an 85-bp ARS1 origin DNA. The structure reveals that Cdc6 contributes to origin DNA recognition via its winged helix domain (WHD) and its initiator-specific motif. Cdc6 binding rearranges a short α-helix in the Orc1 AAA+ domain and the Orc2 WHD, leading to the activation of the Cdc6 ATPase and the formation of the three sites for the recruitment of Mcm2-7, none of which are present in ORC alone. The results illuminate the molecular mechanism of a critical biochemical step in the licensing of eukaryotic replication origins.
History
Deposition
Apr 10, 2021
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Header (metadata) release
Jun 2, 2021
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Map release
Jun 2, 2021
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Update
Aug 4, 2021
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Current status
Aug 4, 2021
Processing site: RCSB / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
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