ジャーナル: J Virol / 年: 2013 タイトル: Structure and functional analysis of the host recognition device of lactococcal phage tuc2009. 著者: Barry Collins / Cecilia Bebeacua / Jennifer Mahony / Stéphanie Blangy / François P Douillard / David Veesler / Christian Cambillau / Douwe van Sinderen / 要旨: Many phages employ a large heteropolymeric organelle located at the tip of the tail, termed the baseplate, for host recognition. Contrast electron microscopy (EM) of the lactococcal phage Tuc2009 ...Many phages employ a large heteropolymeric organelle located at the tip of the tail, termed the baseplate, for host recognition. Contrast electron microscopy (EM) of the lactococcal phage Tuc2009 baseplate and its host-binding subunits, the so-called tripods, allowed us to obtain a low-resolution structural image of this organelle. Structural comparisons between the baseplate of the related phage TP901-1 and that of Tuc2009 demonstrated that they are highly similar, except for the presence of an additional protein in the Tuc2009 baseplate (BppATuc2009), which is attached to the top of the Tuc2009 tripod structure. Recombinantly produced Tuc2009 or TP901-1 tripods were shown to bind specifically to their particular host cell surfaces and are capable of almost fully and specifically eliminating Tuc2009 or TP901-1 phage adsorption, respectively. In the case of Tuc2009, such adsorption-blocking ability was reduced in tripods that lacked BppATuc2009, indicating that this protein increases the binding specificity and/or affinity of the Tuc2009 tripod to its host receptor.
ダウンロード / ファイル: emd_2340.map.gz / 形式: CCP4 / 大きさ: 11.1 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
Reconstruction of the baseplate of lactococcal phage Tuc2009
ボクセルのサイズ
X=Y=Z: 4.95 Å
密度
表面レベル
登録者による: 0.001 / ムービー #1: 0.001
最小 - 最大
-0.0000302 - 0.04787388
平均 (標準偏差)
0.00086621 (±0.00411965)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
-72
-72
-72
サイズ
144
144
144
Spacing
144
144
144
セル
A=B=C: 712.8 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
4.95
4.95
4.95
M x/y/z
144
144
144
origin x/y/z
0.000
0.000
0.000
length x/y/z
712.800
712.800
712.800
α/β/γ
90.000
90.000
90.000
start NX/NY/NZ
-27
-15
-36
NX/NY/NZ
55
31
73
MAP C/R/S
1
2
3
start NC/NR/NS
-72
-72
-72
NC/NR/NS
144
144
144
D min/max/mean
-0.000
0.048
0.001
-
添付データ
-
試料の構成要素
-
全体 : Baseplate of lactoccocal phage Tuc2009
全体
名称: Baseplate of lactoccocal phage Tuc2009
要素
試料: Baseplate of lactoccocal phage Tuc2009
ウイルス: Lactococcus phage Tuc2009 (ファージ)
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超分子 #1000: Baseplate of lactoccocal phage Tuc2009
超分子
名称: Baseplate of lactoccocal phage Tuc2009 / タイプ: sample / ID: 1000 詳細: The baseplate was selected from images collected from a sample of the entire phages. 集合状態: 6 / Number unique components: 1
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超分子 #1: Lactococcus phage Tuc2009
超分子
名称: Lactococcus phage Tuc2009 / タイプ: virus / ID: 1 / Name.synonym: Tuc2009 / NCBI-ID: 35241 / 生物種: Lactococcus phage Tuc2009 / ウイルスタイプ: OTHER / ウイルス・単離状態: SPECIES / ウイルス・エンベロープ: No / ウイルス・中空状態: No / Syn species name: Tuc2009