ジャーナル: J Virol / 年: 2013 タイトル: Structural changes in dengue virus when exposed to a temperature of 37°C. 著者: Guntur Fibriansah / Thiam-Seng Ng / Victor A Kostyuchenko / Jaime Lee / Sumarlin Lee / Jiaqi Wang / Shee-Mei Lok / 要旨: Previous binding studies of antibodies that recognized a partially or fully hidden epitope suggest that insect cell-derived dengue virus undergoes structural changes at an elevated temperature. This ...Previous binding studies of antibodies that recognized a partially or fully hidden epitope suggest that insect cell-derived dengue virus undergoes structural changes at an elevated temperature. This was confirmed by our cryo-electron microscopy images of dengue virus incubated at 37°C, where viruses change their surface from smooth to rough. Here we present the cryo-electron microscopy structures of dengue virus at 37°C. Image analysis showed four classes of particles. The three-dimensional (3D) map of one of these classes, representing half of the imaged virus population, shows that the E protein shell has expanded and there is a hole at the 3-fold vertices. Fitting E protein structures into the map suggests that all of the interdimeric and some intradimeric E protein interactions are weakened. The accessibility of some previously found cryptic epitopes on this class of particles is discussed.
ダウンロード / ファイル: emd_2296.map.gz / 形式: CCP4 / 大きさ: 465.7 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
Reconstruction of one particles class in the "breathing" dengue virus particles
ボクセルのサイズ
X=Y=Z: 1.9 Å
密度
表面レベル
登録者による: 2.0 / ムービー #1: 2
最小 - 最大
-6.12860346 - 10.112843509999999
平均 (標準偏差)
0.0363888 (±0.99221563)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
-250
-250
-250
サイズ
500
500
500
Spacing
500
500
500
セル
A=B=C: 950.0 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
1.9
1.9
1.9
M x/y/z
500
500
500
origin x/y/z
0.000
0.000
0.000
length x/y/z
950.000
950.000
950.000
α/β/γ
90.000
90.000
90.000
start NX/NY/NZ
-36
-30
-80
NX/NY/NZ
73
61
161
MAP C/R/S
1
2
3
start NC/NR/NS
-250
-250
-250
NC/NR/NS
500
500
500
D min/max/mean
-6.129
10.113
0.036
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添付データ
-
試料の構成要素
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全体 : Dengue Virus 2
全体
名称: Dengue Virus 2 (デング熱ウイルス)
要素
試料: Dengue Virus 2
ウイルス: Dengue virus 2 (デング熱ウイルス)
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超分子 #1000: Dengue Virus 2
超分子
名称: Dengue Virus 2 / タイプ: sample / ID: 1000 / 集合状態: full dengue virion / Number unique components: 1
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超分子 #1: Dengue virus 2
超分子
名称: Dengue virus 2 / タイプ: virus / ID: 1 / NCBI-ID: 11060 / 生物種: Dengue virus 2 / Sci species strain: New Guinea C / ウイルスタイプ: VIRION / ウイルス・単離状態: STRAIN / ウイルス・エンベロープ: Yes / ウイルス・中空状態: No
宿主
生物種: Homo sapiens (ヒト) / 別称: VERTEBRATES
Host system
生物種: Aedes albopictus (ヒトスジシマカ) / 組換細胞: C6/36
-
実験情報
-
構造解析
手法
クライオ電子顕微鏡法
解析
単粒子再構成法
試料の集合状態
particle
-
試料調製
緩衝液
pH: 8 / 詳細: 10 mM Tris-HCl, 120 mM NaCl, 1 mM EDTA
グリッド
詳細: lacey carbon grid, layered with ultra thin carbon
凍結
凍結剤: ETHANE / チャンバー内湿度: 100 % / 装置: FEI VITROBOT MARK IV 手法: 2.5 microlitres virus sample was added per grid and blotted with filter paper for 2.5 s before plunging