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- EMDB-22475: Composite cryo-EM density map of the radial spoke 1 isolated from... -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-22475 | |||||||||
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Title | Composite cryo-EM density map of the radial spoke 1 isolated from Chlamydomonas reinhardtii | |||||||||
![]() | composite map | |||||||||
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Function / homology | ![]() radial spoke head / radial spoke / positive regulation of cilium-dependent cell motility / kinocilium / cilium movement involved in cell motility / cilium movement / motile cilium assembly / axoneme assembly / dynein complex / cytoplasmic dynein complex ...radial spoke head / radial spoke / positive regulation of cilium-dependent cell motility / kinocilium / cilium movement involved in cell motility / cilium movement / motile cilium assembly / axoneme assembly / dynein complex / cytoplasmic dynein complex / Set1C/COMPASS complex / ciliary plasm / Oxidoreductases / CTP biosynthetic process / UTP biosynthetic process / motile cilium / GTP biosynthetic process / nucleoside diphosphate kinase activity / dynein intermediate chain binding / axoneme / microtubule-based process / chaperone cofactor-dependent protein refolding / spermatid development / cilium assembly / protein peptidyl-prolyl isomerization / centriole / acrosomal vesicle / peptidylprolyl isomerase / peptidyl-prolyl cis-trans isomerase activity / cilium / unfolded protein binding / protein folding / protein-folding chaperone binding / microtubule / oxidoreductase activity / cytoskeleton / calmodulin binding / phosphorylation / calcium ion binding / ATP binding / membrane / nucleus / metal ion binding / cytoplasm / cytosol Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.2 Å | |||||||||
![]() | Gui M / Ma M / Sze-Tu E / Wang X / Koh F / Zhong E / Berger B / Davis J / Dutcher S / Zhang R / Brown A | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structures of radial spokes and associated complexes important for ciliary motility. Authors: Miao Gui / Meisheng Ma / Erica Sze-Tu / Xiangli Wang / Fujiet Koh / Ellen D Zhong / Bonnie Berger / Joseph H Davis / Susan K Dutcher / Rui Zhang / Alan Brown / ![]() ![]() Abstract: In motile cilia, a mechanoregulatory network is responsible for converting the action of thousands of dynein motors bound to doublet microtubules into a single propulsive waveform. Here, we use two ...In motile cilia, a mechanoregulatory network is responsible for converting the action of thousands of dynein motors bound to doublet microtubules into a single propulsive waveform. Here, we use two complementary cryo-EM strategies to determine structures of the major mechanoregulators that bind ciliary doublet microtubules in Chlamydomonas reinhardtii. We determine structures of isolated radial spoke RS1 and the microtubule-bound RS1, RS2 and the nexin-dynein regulatory complex (N-DRC). From these structures, we identify and build atomic models for 30 proteins, including 23 radial-spoke subunits. We reveal how mechanoregulatory complexes dock to doublet microtubules with regular 96-nm periodicity and communicate with one another. Additionally, we observe a direct and dynamically coupled association between RS2 and the dynein motor inner dynein arm subform c (IDAc), providing a molecular basis for the control of motor activity by mechanical signals. These structures advance our understanding of the role of mechanoregulation in defining the ciliary waveform. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 21.2 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 49.3 KB 49.3 KB | Display Display | ![]() |
Images | ![]() | 69.2 KB | ||
Masks | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() | 669.9 MB 669.9 MB 669.9 MB 669.9 MB 669.9 MB 669.9 MB 669.9 MB 669.9 MB | ![]() | |
Others | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() | 13.6 MB 22.1 MB 6.4 MB 6.9 MB 3.4 MB 2.6 MB 2.4 MB 2.5 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 189.3 KB | Display | ![]() |
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Full document | ![]() | 188.8 KB | Display | |
Data in XML | ![]() | 503 B | Display | |
Data in CIF | ![]() | 372 B | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7jtkMC ![]() 7jtsC ![]() 7ju4C C: citing same article ( M: atomic model generated by this map |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Annotation | composite map | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.09 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
+Mask #1
+Mask #2
+Mask #3
+Mask #4
+Mask #5
+Mask #6
+Mask #7
+Mask #8
+Additional map: #6
+Additional map: #5
+Additional map: #4
+Additional map: #3
+Additional map: #2
+Additional map: #8
+Additional map: #7
+Additional map: #1
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Sample components
+Entire : radial spoke 1
+Supramolecule #1: radial spoke 1
+Macromolecule #1: Flagellar radial spoke protein 1
+Macromolecule #2: Flagellar radial spoke protein 2
+Macromolecule #3: Flagellar radial spoke protein 3
+Macromolecule #4: Flagellar radial spoke protein 4
+Macromolecule #5: Flagellar radial spoke protein 5
+Macromolecule #6: Flagellar radial spoke protein 6
+Macromolecule #7: Flagellar radial spoke protein 7
+Macromolecule #8: Flagellar radial spoke protein 9
+Macromolecule #9: Flagellar radial spoke protein 10
+Macromolecule #10: Flagellar radial spoke protein 11
+Macromolecule #11: Flagellar radial spoke protein 12
+Macromolecule #12: Flagellar radial spoke protein 14
+Macromolecule #13: Flagellar radial spoke protein 16
+Macromolecule #14: Dynein 8 kDa light chain, flagellar outer arm
+Macromolecule #15: Unknown protein
+Macromolecule #16: Flagellar radial spoke protein 23
+Macromolecule #17: Cytochrome b5 heme-binding domain-containing protein
+Macromolecule #18: Uncharacterized protein
+Macromolecule #19: Predicted protein
+Macromolecule #20: PHOSPHATE ION
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 0.75 mg/mL |
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Buffer | pH: 7.4 |
Grid | Pretreatment - Type: GLOW DISCHARGE / Details: unspecified |
Vitrification | Cryogen name: ETHANE / Instrument: FEI VITROBOT MARK IV |
Details | This sample was monodisperse. |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 60.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | C2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal magnification: 81000 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |