ジャーナル: Nanoscale / 年: 2020 タイトル: Truncated tetrahedral RNA nanostructures exhibit enhanced features for delivery of RNAi substrates. 著者: Paul Zakrevsky / Wojciech K Kasprzak / William F Heinz / Weimin Wu / Htet Khant / Eckart Bindewald / Nomongo Dorjsuren / Eric A Fields / Natalia de Val / Luc Jaeger / Bruce A Shapiro / 要旨: Using RNA as a material for nanoparticle construction provides control over particle size and shape at the nano-scale. RNA nano-architectures have shown promise as delivery vehicles for RNA ...Using RNA as a material for nanoparticle construction provides control over particle size and shape at the nano-scale. RNA nano-architectures have shown promise as delivery vehicles for RNA interference (RNAi) substrates, allowing multiple functional entities to be combined on a single particle in a programmable fashion. Rather than employing a completely bottom-up approach to scaffold design, here multiple copies of an existing synthetic supramolecular RNA nano-architecture serve as building blocks along with additional motifs for the design of a novel truncated tetrahedral RNA scaffold, demonstrating that rationally designed RNA assemblies can themselves serve as modular pieces in the construction of larger rationally designed structures. The resulting tetrahedral scaffold displays enhanced characteristics for RNAi-substrate delivery in comparison to similar RNA-based scaffolds, as evidenced by its increased functional capacity, increased cellular uptake and ultimately an increased RNAi efficacy of its adorned Dicer substrate siRNAs. The unique truncated tetrahedral shape of the nanoparticle core appears to contribute to this particle's enhanced function, indicating the physical characteristics of RNA scaffolds merit significant consideration when designing platforms for delivery of functional RNAs via RNA nanoparticles.
ダウンロード / ファイル: emd_21026.map.gz / 形式: CCP4 / 大きさ: 64 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
ボクセルのサイズ
X=Y=Z: 1.36 Å
密度
表面レベル
登録者による: 0.01 / ムービー #1: 0.01
最小 - 最大
-0.0018489175 - 0.021540685
平均 (標準偏差)
0.00016715552 (±0.0018350774)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
0
0
0
サイズ
256
256
256
Spacing
256
256
256
セル
A=B=C: 348.16 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
1.36
1.36
1.36
M x/y/z
256
256
256
origin x/y/z
0.000
0.000
0.000
length x/y/z
348.160
348.160
348.160
α/β/γ
90.000
90.000
90.000
start NX/NY/NZ
-38
-19
-20
NX/NY/NZ
85
80
82
MAP C/R/S
1
2
3
start NC/NR/NS
0
0
0
NC/NR/NS
256
256
256
D min/max/mean
-0.002
0.022
0.000
-
添付データ
-
試料の構成要素
-
全体 : Tetrahedral RNA nanoparticle
全体
名称: Tetrahedral RNA nanoparticle
要素
複合体: Tetrahedral RNA nanoparticle
-
超分子 #1: Tetrahedral RNA nanoparticle
超分子
名称: Tetrahedral RNA nanoparticle / タイプ: complex / ID: 1 / 親要素: 0 詳細: Supra-molecular RNA structure assembled from rationally designed RNA strands produced by in vitro transcription of synthetic DNA templates
由来(天然)
生物種: unidentified, produced by invitro transcription of synthetic templates (unknown)