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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-20685 | |||||||||
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Title | Average of the TZ linker from bovine respiratory cilia | |||||||||
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![]() | Nascent axoneme from bovine respiratory epithelial cells | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | subtomogram averaging / ![]() | |||||||||
![]() | Greenan GA / Vale RD / Agard DA | |||||||||
Funding support | ![]()
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![]() | Journal: To Be Published Title: Defining the transition zone in motile cilia using electron cryo-tomography Authors: Greenan GA / Vale RD / Agard DA | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
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Download
Header (meta data in XML format) |
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Images |
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Archive directory |
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Links
EMDB pages | ![]() ![]() |
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-Related structure data
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 8.18 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire Nascent axoneme from bovine respiratory epithelial cells
Entire | Name: Nascent axoneme from bovine respiratory epithelial cells Number of components: 1 |
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-Component #1: cellular-component, Nascent axoneme from bovine respiratory epith...
Cellular-component | Name: Nascent axoneme from bovine respiratory epithelial cells Recombinant expression: No |
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Source | Species: ![]() ![]() ![]() |
Source (natural) | Organelle: cilium |
-Experimental details
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Sample preparation
Specimen | Specimen state: Filament / Method: ![]() |
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Sample solution | pH: 7.4 |
Vitrification | Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Temperature: 295 K / Humidity: 100 % / Details: 15 second blot. |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Tecnai Polara / Image courtesy: FEI Company |
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![]() | Microscope: FEI POLARA 300 |
Electron gun | Electron source: FIELD EMISSION GUN![]() |
Lens | Imaging mode: BRIGHT FIELD![]() |
Specimen Holder | Model: OTHER |
Camera | Detector: GATAN K2 SUMMIT (4k x 4k) |
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Image processing
![]() | Method: subtomogram averaging / Applied symmetry: C1 (asymmetric) / Number of subtomograms: 824 |
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3D reconstruction | Algorithm: BACK PROJECTION / Resolution: 33.2 Å / Resolution method: FSC 0.143 CUT-OFF |