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TitleElectron cryotomography of intact motile cilia defines the basal body to axoneme transition.
Journal, issue, pagesJ Cell Biol, Vol. 219, Issue 1, Year 2020
Publish dateJan 6, 2020
AuthorsGarrett A Greenan / Ronald D Vale / David A Agard /
PubMed AbstractCells use motile cilia to generate force in the extracellular space. The structure of a cilium can be classified into three subdomains: the intracellular basal body (BB) that templates cilium ...Cells use motile cilia to generate force in the extracellular space. The structure of a cilium can be classified into three subdomains: the intracellular basal body (BB) that templates cilium formation, the extracellular axoneme that generates force, and the transition zone (TZ) that bridges them. While the BB is composed of triplet microtubules (TMTs), the axoneme is composed of doublet microtubules (DMTs), meaning the cilium must convert between different microtubule geometries. Here, we performed electron cryotomography to define this conversion, and our reconstructions reveal identifying structural features of the BB, TZ, and axoneme. Each region is distinct in terms of microtubule number and geometry, microtubule inner proteins, and microtubule linkers. TMT to DMT conversion occurs within the BB, and microtubule geometry changes to axonemal by the end of the TZ, followed by the addition of axoneme-specific components essential for cilium motility. Our results provide the highest-resolution images of the motile cilium to date and reveal how BBs template axonemes.
External linksJ Cell Biol / PubMed:31874113 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution30.7 - 35.6 Å
Structure data

EMDB-20674:
Nascent axoneme from bovine respiratory epithelial cells
Method: EM (subtomogram averaging) / Resolution: 35.6 Å

EMDB-20675:
Whole-population average of the first 800 nm of the bovine respiratory epithelium
Method: EM (subtomogram averaging) / Resolution: 30.7 Å

EMDB-20676:
Average of the basal body from bovine respiratory cilia
Method: EM (subtomogram averaging) / Resolution: 31.5 Å

EMDB-20679:
Average of the transition zone from bovine respiratory cilia
Method: EM (subtomogram averaging) / Resolution: 31.5 Å

EMDB-20680:
Average of the proximal axoneme from bovine respiratory cilia
Method: EM (subtomogram averaging) / Resolution: 35.1 Å

EMDB-20681:
Average of the proximal basal body from bovine respiratory cilia
Method: EM (subtomogram averaging) / Resolution: 33.2 Å

EMDB-20683:
Average of the mid basal body from bovine respiratory cilia
Method: EM (subtomogram averaging) / Resolution: 33.2 Å

EMDB-20684:
Average of the distal basal body from bovine respiratory cilia
Method: EM (subtomogram averaging) / Resolution: 32.3 Å

EMDB-20685:
Average of the TZ linker from bovine respiratory cilia
Method: EM (subtomogram averaging) / Resolution: 33.2 Å

Source
  • Bos taurus (cattle)

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