- EMDB-20164: The central pair apparatus focusing on the C1a-e-c supercomplex e... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-20164
タイトル
The central pair apparatus focusing on the C1a-e-c supercomplex extracted from the cryo-electron tomography and subtomographic average of isolated Chlamydomonas fap81 mutant axoneme
マップデータ
The central pair apparatus focusing on the C1a-e-c supercomplex extracted from the cryo-electron tomography and subtomographic average of isolated Chlamydomonas fap81 mutant axoneme
試料
複合体: The C1a-e-c supercomplex of central pair apparatus averaged from Chlamydomonas fap81 mutant cilia
National Institutes of Health/National Institute of General Medical Sciences
R01GM083122
米国
National Institutes of Health/National Institute of General Medical Sciences
R37GM030626
米国
National Institutes of Health/National Institute of General Medical Sciences
R01GM112050
米国
National Institutes of Health/National Institute of General Medical Sciences
R35GM122574
米国
引用
ジャーナル: J Cell Biol / 年: 2019 タイトル: Structural organization of the C1a-e-c supercomplex within the ciliary central apparatus. 著者: Gang Fu / Lei Zhao / Erin Dymek / Yuqing Hou / Kangkang Song / Nhan Phan / Zhiguo Shang / Elizabeth F Smith / George B Witman / Daniela Nicastro / 要旨: Nearly all motile cilia contain a central apparatus (CA) composed of two connected singlet microtubules with attached projections that play crucial roles in regulating ciliary motility. Defects in CA ...Nearly all motile cilia contain a central apparatus (CA) composed of two connected singlet microtubules with attached projections that play crucial roles in regulating ciliary motility. Defects in CA assembly usually result in motility-impaired or paralyzed cilia, which in humans causes disease. Despite their importance, the protein composition and functions of the CA projections are largely unknown. Here, we integrated biochemical and genetic approaches with cryo-electron tomography to compare the CA of wild-type with CA mutants. We identified a large (>2 MD) complex, the C1a-e-c supercomplex, that requires the PF16 protein for assembly and contains the CA components FAP76, FAP81, FAP92, and FAP216. We localized these subunits within the supercomplex using nanogold labeling and show that loss of any one of them results in impaired ciliary motility. These data provide insight into the subunit organization and 3D structure of the CA, which is a prerequisite for understanding the molecular mechanisms by which the CA regulates ciliary beating.
ダウンロード / ファイル: emd_20164.map.gz / 形式: CCP4 / 大きさ: 3.7 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
The central pair apparatus focusing on the C1a-e-c supercomplex extracted from the cryo-electron tomography and subtomographic average of isolated Chlamydomonas fap81 mutant axoneme
ボクセルのサイズ
X=Y=Z: 5.523 Å
密度
表面レベル
登録者による: 123 / ムービー #1: 123
最小 - 最大
112.4079 - 134.06241
平均 (標準偏差)
120.77883 (±3.094791)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
-19
-102
-19
サイズ
90
90
120
Spacing
90
90
120
セル
A: 497.06998 Å / B: 497.06998 Å / C: 662.75995 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
5.523
5.523
5.523
M x/y/z
90
90
120
origin x/y/z
0.000
0.000
0.000
length x/y/z
497.070
497.070
662.760
α/β/γ
90.000
90.000
90.000
start NX/NY/NZ
0
0
0
NX/NY/NZ
254
265
109
MAP C/R/S
1
2
3
start NC/NR/NS
-102
-19
-19
NC/NR/NS
90
90
120
D min/max/mean
112.408
134.062
120.779
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添付データ
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試料の構成要素
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全体 : The C1a-e-c supercomplex of central pair apparatus averaged from ...
全体
名称: The C1a-e-c supercomplex of central pair apparatus averaged from Chlamydomonas fap81 mutant cilia
要素
複合体: The C1a-e-c supercomplex of central pair apparatus averaged from Chlamydomonas fap81 mutant cilia
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超分子 #1: The C1a-e-c supercomplex of central pair apparatus averaged from ...
超分子
名称: The C1a-e-c supercomplex of central pair apparatus averaged from Chlamydomonas fap81 mutant cilia タイプ: complex / ID: 1 / 親要素: 0