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- EMDB-19885: Low-dose cryo-electron ptychographic reconstruction of TMV record... -
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Open data
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Basic information
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Title | Low-dose cryo-electron ptychographic reconstruction of TMV recorded with CSA of 6.1 mrad | |||||||||
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![]() | Capsid / Tobacco Mosaic Virus / VIRUS | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | helical reconstruction / cryo EM / Resolution: 6.4 Å | |||||||||
![]() | Mohammed I / Stalhberg H / Kucukoglu B | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Low-dose cryo-electron ptychography of proteins at sub-nanometer resolution Authors: Mohammed I / Stalhberg H / Kucukoglu B | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 15.8 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 12.7 KB 12.7 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 12.1 KB | Display | ![]() |
Images | ![]() | 73 KB | ||
Masks | ![]() | 149.9 MB | ![]() | |
Filedesc metadata | ![]() | 4 KB | ||
Others | ![]() ![]() | 118.7 MB 118.5 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Voxel size | X=Y=Z: 1.1659 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Half map: #1
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Density Histograms |
-Half map: #2
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Tobacco mosaic virus
Entire | Name: ![]() ![]() |
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Components |
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-Supramolecule #1: Tobacco mosaic virus
Supramolecule | Name: Tobacco mosaic virus / type: virus / ID: 1 / Parent: 0 / NCBI-ID: 12242 / Sci species name: Tobacco mosaic virus / Virus type: VIRION / Virus isolate: OTHER / Virus enveloped: Yes / Virus empty: No |
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Molecular weight | Theoretical: 40.0 MDa |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | helical reconstruction |
Aggregation state | filament |
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Sample preparation
Buffer | pH: 7.4 |
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Grid | Model: Quantifoil R1.2/1.3 / Material: GOLD / Mesh: 300 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 60 sec. |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 283 K / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: DECTRIS ELA (1k x 0.5k) / Average electron dose: 32.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: SPOT SCAN / Imaging mode: DIFFRACTION / Cs: 0.1 mm / Nominal defocus max: 1.8 µm / Nominal defocus min: 1.4000000000000001 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |