+
データを開く
-
基本情報
登録情報 | ![]() | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
タイトル | Human pre-60S - State 5 | |||||||||
![]() | Human pre-60S - State 5 - Composite map | |||||||||
![]() |
| |||||||||
![]() | Human / ribosome biogenesis / RIX1 complex / 5S RNP / RIBOSOME | |||||||||
機能・相同性 | ![]() negative regulation of RNA polymerase II regulatory region sequence-specific DNA binding / positive regulation of protein localization to chromosome, telomeric region / basal RNA polymerase II transcription machinery binding / : / hematopoietic stem cell homeostasis / inner cell mass cell differentiation / dendrite extension / preribosome binding / regulation of Notch signaling pathway / lamin filament ...negative regulation of RNA polymerase II regulatory region sequence-specific DNA binding / positive regulation of protein localization to chromosome, telomeric region / basal RNA polymerase II transcription machinery binding / : / hematopoietic stem cell homeostasis / inner cell mass cell differentiation / dendrite extension / preribosome binding / regulation of Notch signaling pathway / lamin filament / regulation of fatty acid biosynthetic process / regulation of megakaryocyte differentiation / positive regulation of protein sumoylation / miRNA-mediated post-transcriptional gene silencing / miRNA-mediated gene silencing by inhibition of translation / stem cell division / eukaryotic 80S initiation complex / negative regulation of protein neddylation / regulation of G1 to G0 transition / axial mesoderm development / negative regulation of formation of translation preinitiation complex / protein localization to nucleolus / ribosomal protein import into nucleus / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / regulation of translation involved in cellular response to UV / protein-DNA complex disassembly / 90S preribosome assembly / GAIT complex / A band / positive regulation of DNA damage response, signal transduction by p53 class mediator / TORC2 complex binding / alpha-beta T cell differentiation / G1 to G0 transition / regulation of cyclin-dependent protein serine/threonine kinase activity / skeletal system morphogenesis / middle ear morphogenesis / regulation of glycolytic process / translation at presynapse / regulation of reactive oxygen species metabolic process / negative regulation of cell-cell adhesion / positive regulation of dendritic spine development / cytoplasmic side of rough endoplasmic reticulum membrane / mitotic metaphase chromosome alignment / maturation of 5.8S rRNA / negative regulation of ubiquitin protein ligase activity / stem cell population maintenance / homeostatic process / response to aldosterone / negative regulation of DNA replication / macrophage chemotaxis / lung morphogenesis / positive regulation of telomere maintenance / ribosomal large subunit binding / Protein hydroxylation / preribosome, large subunit precursor / Peptide chain elongation / nuclear-transcribed mRNA catabolic process / Selenocysteine synthesis / positive regulation of signal transduction by p53 class mediator / Formation of a pool of free 40S subunits / negative regulation of mitotic cell cycle / ubiquitin ligase inhibitor activity / Eukaryotic Translation Termination / Response of EIF2AK4 (GCN2) to amino acid deficiency / SRP-dependent cotranslational protein targeting to membrane / blastocyst development / cellular response to actinomycin D / negative regulation of ubiquitin-dependent protein catabolic process / Viral mRNA Translation / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / protein localization to nucleus / GTP hydrolysis and joining of the 60S ribosomal subunit / L13a-mediated translational silencing of Ceruloplasmin expression / somitogenesis / Major pathway of rRNA processing in the nucleolus and cytosol / protein targeting / ribosomal subunit export from nucleus / hematopoietic progenitor cell differentiation / protein-RNA complex assembly / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / maturation of LSU-rRNA / rough endoplasmic reticulum / Notch signaling pathway / negative regulation of proteasomal ubiquitin-dependent protein catabolic process / MDM2/MDM4 family protein binding / cytosolic ribosome / negative regulation of protein ubiquitination / translation initiation factor activity / cellular response to interleukin-4 / ossification / innate immune response in mucosa / assembly of large subunit precursor of preribosome / DNA damage response, signal transduction by p53 class mediator / negative regulation of cell migration / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / condensed nuclear chromosome / ribosomal large subunit biogenesis / cytosolic ribosome assembly / regulation of signal transduction by p53 class mediator 類似検索 - 分子機能 | |||||||||
生物種 | ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.84 Å | |||||||||
![]() | Thoms M / Denk T / Beckmann R | |||||||||
資金援助 | European Union, 1件
| |||||||||
![]() | ジャーナル: Acta Crystallogr D Struct Biol / 年: 2018 タイトル: Real-space refinement in PHENIX for cryo-EM and crystallography. 著者: Pavel V Afonine / Billy K Poon / Randy J Read / Oleg V Sobolev / Thomas C Terwilliger / Alexandre Urzhumtsev / Paul D Adams / ![]() ![]() ![]() 要旨: This article describes the implementation of real-space refinement in the phenix.real_space_refine program from the PHENIX suite. The use of a simplified refinement target function enables very fast ...This article describes the implementation of real-space refinement in the phenix.real_space_refine program from the PHENIX suite. The use of a simplified refinement target function enables very fast calculation, which in turn makes it possible to identify optimal data-restraint weights as part of routine refinements with little runtime cost. Refinement of atomic models against low-resolution data benefits from the inclusion of as much additional information as is available. In addition to standard restraints on covalent geometry, phenix.real_space_refine makes use of extra information such as secondary-structure and rotamer-specific restraints, as well as restraints or constraints on internal molecular symmetry. The re-refinement of 385 cryo-EM-derived models available in the Protein Data Bank at resolutions of 6 Å or better shows significant improvement of the models and of the fit of these models to the target maps. | |||||||||
履歴 |
|
-
構造の表示
添付画像 |
---|
-
ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 18.1 MB | ![]() | |
---|---|---|---|---|
ヘッダ (付随情報) | ![]() ![]() | 72.5 KB 72.5 KB | 表示 表示 | ![]() |
画像 | ![]() | 94.4 KB | ||
Filedesc metadata | ![]() | 16.9 KB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 8rl2MC ![]() 8ql1C ![]() 9qiwC C: 同じ文献を引用 ( M: このマップから作成された原子モデル |
---|---|
類似構造データ | 類似検索 - 機能・相同性 ![]() |
-
リンク
EMDBのページ | ![]() ![]() |
---|---|
「今月の分子」の関連する項目 |
-
マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
注釈 | Human pre-60S - State 5 - Composite map | ||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.045 Å | ||||||||||||||||||||||||||||||||||||
密度 |
| ||||||||||||||||||||||||||||||||||||
対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
|
-添付データ
-
試料の構成要素
+全体 : Human pre-60S - State 5
+超分子 #1: Human pre-60S - State 5
+分子 #1: 28S rRNA
+分子 #2: 5S rRNA
+分子 #3: 5.8S rRNA
+分子 #4: 60S ribosomal protein L8
+分子 #5: 60S ribosomal protein L3
+分子 #6: 60S ribosomal protein L4
+分子 #7: 60S ribosomal protein L5
+分子 #8: 60S ribosomal protein L6
+分子 #9: 60S ribosomal protein L7
+分子 #10: 60S ribosomal protein L7a
+分子 #11: 60S ribosomal protein L9
+分子 #12: 60S ribosomal protein L11
+分子 #13: 60S ribosomal protein L13
+分子 #14: 60S ribosomal protein L14
+分子 #15: 60S ribosomal protein L15
+分子 #16: 60S ribosomal protein L13a
+分子 #17: 60S ribosomal protein L17
+分子 #18: 60S ribosomal protein L18
+分子 #19: 60S ribosomal protein L19
+分子 #20: 60S ribosomal protein L18a
+分子 #21: 60S ribosomal protein L21
+分子 #22: 60S ribosomal protein L22
+分子 #23: 60S ribosomal protein L23
+分子 #24: 60S ribosomal protein L23a
+分子 #25: 60S ribosomal protein L26
+分子 #26: 60S ribosomal protein L27
+分子 #27: 60S ribosomal protein L27a
+分子 #28: 60S ribosomal protein L30
+分子 #29: 60S ribosomal protein L31
+分子 #30: 60S ribosomal protein L32
+分子 #31: 60S ribosomal protein L35a
+分子 #32: 60S ribosomal protein L34
+分子 #33: 60S ribosomal protein L35
+分子 #34: 60S ribosomal protein L36
+分子 #35: 60S ribosomal protein L37
+分子 #36: 60S ribosomal protein L38
+分子 #37: 60S ribosomal protein L39
+分子 #38: 60S ribosomal protein L37a
+分子 #39: 60S ribosomal protein L28
+分子 #40: Large ribosomal subunit protein uL11
+分子 #41: Eukaryotic translation initiation factor 6
+分子 #42: Nucleolar GTP-binding protein 2
+分子 #43: Guanine nucleotide-binding protein-like 3
+分子 #44: GTP-binding protein 4
+分子 #45: Protein LLP homolog
+分子 #46: mRNA turnover protein 4 homolog
+分子 #47: Notchless protein homolog 1
+分子 #48: Ribosome biogenesis protein NSA2 homolog
+分子 #49: Probable ribosome biogenesis protein RLP24
+分子 #50: Ribosome production factor 2 homolog
+分子 #51: Ribosome biogenesis regulatory protein homolog
+分子 #52: Zinc finger protein 593
+分子 #53: ZINC ION
+分子 #54: GUANOSINE-5'-TRIPHOSPHATE
+分子 #55: MAGNESIUM ION
+分子 #56: GUANOSINE-5'-DIPHOSPHATE
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
---|---|
![]() | 単粒子再構成法 |
試料の集合状態 | particle |
-
試料調製
緩衝液 | pH: 7.5 |
---|---|
凍結 | 凍結剤: ETHANE |
-
電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
---|---|
撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 平均電子線量: 43.6 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: SPOT SCAN / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 3.5 µm / 最小 デフォーカス(公称値): 0.5 µm |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
-
画像解析
初期モデル | モデルのタイプ: NONE |
---|---|
最終 再構成 | 解像度のタイプ: BY AUTHOR / 解像度: 2.84 Å / 解像度の算出法: FSC 0.143 CUT-OFF 詳細: Resolution given for consensus refinement - this is a composite map 使用した粒子像数: 78099 |
初期 角度割当 | タイプ: MAXIMUM LIKELIHOOD |
最終 角度割当 | タイプ: MAXIMUM LIKELIHOOD |