PDB-8ql1: Crystal structure of the human MDN1-MIDAS/NLE1-UBL complex

ID or keywords:

Entry

Database: PDB / ID: 8ql1

Title

Crystal structure of the human MDN1-MIDAS/NLE1-UBL complex

Components

NDE1
Notchless protein homolog 1

Keywords

RIBOSOME / AAA+ ATPase / Metal ion dependent adhesion site (MIDAS) / Ubiquitin-like domain (Ubl) / pre-60S biogenesis

Function / homology

Function and homology information

hematopoietic stem cell homeostasis / inner cell mass cell differentiation / regulation of Notch signaling pathway / skeletal system morphogenesis / negative regulation of mitotic cell cycle / somitogenesis / Notch signaling pathway / ribosomal large subunit biogenesis / kidney development / positive regulation of canonical Wnt signaling pathway ...
Similarity search - Function

Biological species

Homo sapiens (human)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 2.3 Å

Authors

Wild, K. / Fiorentino, F. / Hurt, E. / Sinning, I.

Funding support

Germany, 1items

Citation

Journal: Nucleic Acids Res / Year: 2025
Title: Highly conserved ribosome biogenesis pathways between human and yeast revealed by the MDN1-NLE1 interaction and NLE1 containing pre-60S subunits.
Authors: Federica Fiorentino / Matthias Thoms / Klemens Wild / Timo Denk / Jingdong Cheng / Jakub Zeman / Irmgard Sinning / Ed Hurt / Roland Beckmann /

Abstract: The assembly of ribosomal subunits, primarily occurring in the nucleolar and nuclear compartments, is a highly complex process crucial for cellular function. This study reveals the conservation of ...

History

Deposition	Sep 19, 2023	Deposition site: PDBE / Processing site: PDBE
Revision 1.0	Apr 9, 2025	Provider: repository / Type: Initial release
Revision 1.1	Apr 30, 2025	Group: Database references / Category: citation / citation_author Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	8ql1.cif.gz	543.8 KB	Display	PDBx/mmCIF format
PDB format	pdb8ql1.ent.gz	448 KB	Display	PDB format
PDBx/mmJSON format	8ql1.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/ql/8ql1 ftp://data.pdbj.org/pub/pdb/validation_reports/ql/8ql1	HTTPS FTP

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Related structure data

Related structure data	19287C 19288C 19289C 19290C 19291C 19292C 19293C 19294C 19295C 19296C 19297C 19298C 19299C 19300C 19301C 19302C 19303C 19304C 19305C 19306C 19307C 19308C 19309C 19310C 19311C 19312C 19313C 19314C 19315C 19316C 19317C 19318C 19319C 19320C 19321C 19322C 19323C 19324C 19325C 19330C 53196C 8rl2C 9qiwC C: citing same article (ref.)
Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#10 - Oct 2000 Ribosome similarity (1) #58 - Oct 2004 G Proteins similarity (1) #164 - Aug 2013 Serotonin Receptor similarity (1)

Deposited unit

A: NDE1

B: Notchless protein homolog 1

C: NDE1

D: Notchless protein homolog 1

E: NDE1

F: Notchless protein homolog 1

G: NDE1

H: Notchless protein homolog 1

hetero molecules

172 kDa, 8 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: NDE1

B: Notchless protein homolog 1

hetero molecules

defined by author
Evidence: gel filtration
43.1 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

C: NDE1

D: Notchless protein homolog 1

hetero molecules

defined by author
43.1 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

3

E: NDE1

F: Notchless protein homolog 1

hetero molecules

defined by author
43.1 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

4

G: NDE1

H: Notchless protein homolog 1

hetero molecules

defined by author
43.1 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	237.160, 69.818, 103.183
Angle α, β, γ (deg.)	90.00, 91.79, 90.00
Int Tables number	5
Space group name H-M	C121

Components on special symmetry positions

ID	Model	Components
1	1	F-223- HOH

#1: Protein	NDE1 Mass: 31597.990 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli)
#2: Protein	Notchless protein homolog 1 Mass: 11496.986 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: NLE1, HUSSY-07 / Production host: Escherichia coli (E. coli) / References: UniProt: Q9NVX2
#3: Chemical	ChemComp-MG / MAGNESIUM ION Mass: 24.305 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Mg / Feature type: SUBJECT OF INVESTIGATION
#4: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 415 / Source method: isolated from a natural source / Formula: H₂O
Has ligand of interest	Y
Has protein modification	N

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.48 Å³/Da / Density % sol: 50.34 %
Crystal grow	Temperature: 277 K / Method: vapor diffusion, sitting drop Details: 0.2 M Ammonium sulfate, 18.2% v/v Polyethylene glycol 300, 0.1 M Sodium Acetate, 3.5 M Ammonium chloride

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Data collection

Diffraction	Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: PETRA III, EMBL c/o DESY / Beamline: P13 (MX1) / Wavelength: 0.9625 Å
Detector	Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Mar 26, 2019
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 0.9625 Å / Relative weight: 1
Reflection	Resolution: 2.3→118.522 Å / Num. obs: 75310 / % possible obs: 99.6 % / Redundancy: 6.6 % / CC_1/2: 0.981 / R_pim(I) all: 0.074 / Net I/σ(I): 8.9
Reflection shell	Resolution: 2.3→2.38 Å / Num. unique obs: 7442 / CC_1/2: 0.675

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Processing

Software

Name	Version	Classification
PHENIX	(1.19_4092: ???)	refinement
XDS		data reduction
Aimless		data scaling
PHENIX		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT / Resolution: 2.3→118.52 Å / SU ML: 0.33 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 28.48 / Stereochemistry target values: ML

	R_factor	Num. reflection	% reflection	Selection details
R_free	0.2476	3805	5.07 %	0
R_work	0.2144	-	-	-
obs	0.2161	75008	99.6 %	-

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Refinement step

Cycle: LAST / Resolution: 2.3→118.52 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	10351	0	4	415	10770

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.003	10537
X-RAY DIFFRACTION	f_angle_d	0.558	14283
X-RAY DIFFRACTION	f_dihedral_angle_d	15.074	3921
X-RAY DIFFRACTION	f_chiral_restr	0.043	1662
X-RAY DIFFRACTION	f_plane_restr	0.004	1854

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
2.3-2.33	0.4019	151	0.3512	2616	X-RAY DIFFRACTION	100
2.33-2.36	0.3382	136	0.3232	2580	X-RAY DIFFRACTION	100
2.36-2.39	0.3503	146	0.3103	2654	X-RAY DIFFRACTION	100
2.39-2.43	0.3466	141	0.2932	2607	X-RAY DIFFRACTION	100
2.43-2.46	0.3326	128	0.2816	2613	X-RAY DIFFRACTION	100
2.46-2.5	0.2938	125	0.2655	2640	X-RAY DIFFRACTION	100
2.5-2.54	0.2511	120	0.2562	2674	X-RAY DIFFRACTION	100
2.54-2.59	0.295	126	0.2529	2653	X-RAY DIFFRACTION	99
2.59-2.63	0.32	149	0.2579	2599	X-RAY DIFFRACTION	100
2.63-2.68	0.324	137	0.2669	2643	X-RAY DIFFRACTION	100
2.68-2.74	0.2944	133	0.275	2599	X-RAY DIFFRACTION	100
2.74-2.8	0.3062	118	0.2686	2672	X-RAY DIFFRACTION	100
2.8-2.86	0.3422	141	0.2763	2628	X-RAY DIFFRACTION	100
2.86-2.93	0.3156	128	0.2572	2662	X-RAY DIFFRACTION	100
2.93-3.01	0.2774	160	0.2441	2604	X-RAY DIFFRACTION	100
3.01-3.1	0.2538	138	0.2266	2649	X-RAY DIFFRACTION	100
3.1-3.2	0.2703	134	0.2257	2654	X-RAY DIFFRACTION	100
3.2-3.32	0.2552	143	0.2335	2622	X-RAY DIFFRACTION	100
3.32-3.45	0.2625	171	0.2289	2613	X-RAY DIFFRACTION	100
3.45-3.61	0.2558	146	0.2087	2639	X-RAY DIFFRACTION	100
3.61-3.8	0.2387	129	0.2106	2624	X-RAY DIFFRACTION	99
3.8-4.04	0.1945	145	0.1837	2652	X-RAY DIFFRACTION	100
4.04-4.35	0.1906	153	0.1556	2663	X-RAY DIFFRACTION	100
4.35-4.78	0.1877	128	0.1484	2669	X-RAY DIFFRACTION	100
4.78-5.48	0.2051	184	0.1664	2614	X-RAY DIFFRACTION	100
5.48-6.9	0.2292	165	0.1938	2677	X-RAY DIFFRACTION	99
6.9-118.52	0.189	130	0.1761	2683	X-RAY DIFFRACTION	96

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	0.5718	-0.6982	0.5424	1.2879	-1.3468	1.677	0.0096	-0.0691	0.8457	-0.3215	0.4117	-1.0924	-0.7126	0.31	-0.2203	0.5153	-0.0496	0.2137	0.4013	-0.0824	0.9745	90.1659	40.9183	81.4158
2	5.4562	0.0044	-1.1191	1.5728	-0.9081	3.0026	0.1845	0.1316	0.4476	-0.1717	-0.0364	-0.2679	0.0294	0.0575	-0.2444	0.3569	-0.016	0.0497	0.2347	-0.0044	0.4534	80.7106	36.6334	80.7273
3	3.0694	0.3124	-0.7822	2.2409	0.347	4.2782	0.0481	0.2534	0.6568	-0.4449	0.1364	-0.7304	-0.5515	0.1527	-0.2062	0.4888	-0.0258	0.1522	0.3188	0.0622	0.628	76.5461	44.4302	76.4872
4	2.2698	0.0114	-1.1036	1.6508	0.6174	5.9329	0.0501	0.3932	0.0684	-0.3023	0.0868	-0.2609	-0.1243	-0.0717	0.0288	0.3906	0.0004	0.0647	0.4067	0.0276	0.4782	67.306	35.2843	71.043
5	2.7329	-0.0315	0.4169	2.5078	0.6151	4.0854	-0.0879	0.1907	-0.096	0.002	-0.1863	0.1304	0.0296	-0.3648	0.0273	0.2981	-0.0136	0.0663	0.2371	-0.0055	0.343	70.1008	33.0897	78.5716
6	4.3631	1.1804	-4.9294	3.2022	-0.4246	5.8536	-0.4748	-0.0961	-1.0804	0.2647	-0.3964	0.0795	1.1612	0.4192	0.8963	0.3687	-0.0032	-0.0425	0.4235	-0.0054	0.3183	61.308	26.9837	77.9877
7	5.6129	-0.1842	0.9496	2.1308	-1.3659	1.0115	-0.0232	-0.6633	0.2362	0.2094	0.0288	0.05	-0.0434	0.3676	-0.1209	0.2715	-0.0511	0.0692	0.2846	-0.0872	0.3836	73.4869	30.613	90.4679
8	4.9905	-0.0146	-1.6132	1.8542	1.4101	4.2501	-0.3775	-0.1022	-0.7927	0.0375	-0.0792	0.2304	0.4689	-0.6106	0.3766	0.321	0.0109	0.0531	0.317	0.0022	0.3565	64.0066	23.8389	94.6598
9	4.7341	-0.9735	2.27	1.2997	-0.0491	8.4183	0.1997	0.3526	-0.2926	-0.1649	-0.2688	-0.1116	0.9866	0.0776	-0.1312	0.4252	0.02	0.1206	0.3098	-0.0409	0.4347	83.5136	23.6374	79.6897
10	5.7418	-1.1872	0.873	4.9891	-0.1164	1.6123	-0.132	-0.5004	0.2946	0.2808	-0.1583	-0.0064	0.4493	-0.794	0.3334	0.2398	-0.002	0.0723	0.3635	-0.0704	0.3941	58.7231	34.528	101.7912
11	5.0588	-2.8365	-0.3119	6.0052	0.4931	2.9097	-0.213	-0.16	-0.3532	0.5642	0.1374	0.3506	-0.0368	0.1956	0.1539	0.2789	0.003	0.0724	0.3041	-0.049	0.3306	51.6959	31.7911	106.9792
12	6.7563	-0.8534	-4.465	3.2254	0.7341	6.7063	0.149	-0.2711	0.4256	0.163	0.1822	0.2512	-0.2214	0.1146	-0.3067	0.2654	0.0081	0.0292	0.243	-0.0424	0.3379	51.5108	40.5738	105.888
13	1.431	0.5503	-0.4991	2.3059	0.0385	2.4067	0.0039	-0.5707	-0.0374	0.3065	-0.0737	-0.3204	0.0506	0.1225	0.0878	0.3068	0.0173	0.0365	0.323	0.0063	0.3751	86.6821	13.1083	101.3308
14	3.0952	-0.6859	0.8209	2.0669	0.2776	2.9771	-0.088	-0.5443	-0.7927	0.0468	-0.0187	-0.1859	0.5528	0.047	0.1681	0.4471	0.047	0.1134	0.3798	0.0706	0.6967	92.3553	3.3519	95.8191
15	4.1599	-0.1946	-0.9237	1.8506	0.2104	2.0402	-0.0459	0.2496	-0.0135	-0.4046	0.1243	-0.5431	0.0136	0.2261	-0.0516	0.3918	-0.0069	0.1535	0.3284	-0.0422	0.4999	94.1292	15.322	81.9851
16	0.0231	-0.2096	-0.2332	2.1648	3.4435	7.3109	-0.0837	0.3699	-0.0177	-0.2576	-0.0743	-0.7339	-0.0093	0.3173	-0.0375	0.3552	-0.0218	0.2395	0.523	-0.1476	0.9466	112.1612	12.5969	80.1884
17	4.9666	0.2638	2.1775	0.9739	-0.2048	4.1181	-0.1466	0.6118	1.0444	0.4018	0.0775	-2.0328	-0.3586	0.9879	-0.0343	0.5353	-0.1013	0.2898	0.6296	-0.0742	1.6008	118.4898	26.2279	84.2268
18	7.4405	1.4714	-0.0862	1.426	1.4176	2.5365	-0.2794	0.854	0.6664	-0.6168	-0.2363	-0.5886	-0.518	0.4145	-0.9741	0.3958	-0.089	0.8655	0.6964	-0.0264	1.5769	119.2751	20.4075	73.1413
19	1.0678	1.2611	0.783	5.7507	-1.0127	6.7061	0.2251	-0.353	-0.7303	-0.4996	0.1293	-1.6977	0.12	0.772	-0.2961	0.5702	0.1386	0.219	0.7975	-0.0336	1.6341	124.1034	9.3133	81.4681
20	1.6179	1.1254	-0.7771	2.1181	-1.114	5.144	0.4516	-1.1253	-0.0104	0.1167	-0.282	-0.4925	0.3163	1.4728	0.1776	0.4928	-0.0099	0.2861	0.7467	-0.0957	1.5191	119.6994	15.5244	85.4585
21	1.6602	-1.0282	1.174	1.1728	-0.6984	2.9629	0.1189	-0.6073	0.1652	0.3235	0.0189	-0.0384	-0.1105	-0.2904	-0.0777	0.3473	-0.0855	0.06	0.4249	-0.0133	0.4775	87.0997	49.4399	57.2942
22	4.2782	0.1022	0.3467	1.1557	-0.0364	2.1866	0.1235	-0.3985	-0.2018	0.0032	-0.0923	-0.2698	0.2927	0.0884	-0.0307	0.3347	0.0232	0.0453	0.2577	0.0468	0.4066	91.6226	40.6342	48.6614
23	5.8536	0.4217	0.1436	0.7971	0.2599	2.7919	0.0935	-0.2001	-0.4768	-0.2204	-0.0462	-0.683	0.1902	0.2392	-0.0074	0.3462	0.0409	0.0643	0.2275	0.0049	0.4176	95.079	41.1986	38.5984
24	2.8877	3.329	-1.4296	8.4558	1.011	5.5015	-0.5504	1.3479	-0.2599	-0.3068	0.4919	0.1648	0.2568	0.1392	-0.066	0.4979	0.0389	0.1407	0.3887	-0.0781	0.617	97.8435	37.1225	29.2525
25	3.5713	-2.2859	-3.4285	2.5246	1.3041	5.6645	0.3494	-0.5311	0.6989	0.2282	0.1082	-0.1277	-0.2143	0.5656	-0.3111	0.2414	-0.0017	0.0582	0.2692	-0.0016	0.4029	98.6372	52.1393	38.894
26	5.167	0.9361	-0.4597	1.4374	-0.4599	1.2974	-0.0293	0.7658	0.2973	-0.2354	0.0525	-0.2759	0.0925	0.1268	-0.0172	0.3359	0.0327	0.0882	0.2856	0.0336	0.4415	96.2819	52.436	32.1164
27	0.2602	-0.7125	-0.3983	5.8885	6.62	9.2222	-0.2264	0.7627	-0.2242	0.2274	-0.0902	0.2656	0.5595	-0.2292	0.265	0.2593	0.0164	0.127	0.4313	0.0043	0.6046	115.1512	47.5085	33.0826
28	3.7567	-0.8843	-1.7249	4.6994	0.842	3.3975	0.0514	0.345	0.6964	-0.097	0.2791	-0.6322	0.0413	0.5543	-0.0982	0.3248	-0.0607	0.1831	0.4269	0.0241	0.7398	120.2595	55.189	30.0598
29	5.0361	-4.8794	1.6278	8.1452	-2.0218	1.9294	0.3475	0.1973	1.5254	-0.521	-0.479	-0.5533	-0.641	0.0283	0.1385	0.5232	-0.0828	0.1126	0.7317	-0.0087	0.9643	127.693	60.5604	26.0375
30	9.6913	-0.1354	-0.6241	7.0849	-1.676	9.1661	0.0454	0.0652	-0.9377	-0.4483	-0.0958	-0.841	-0.3788	0.2625	0.1766	0.3824	0.0124	0.0413	0.4684	-0.0433	0.6099	126.8637	44.2513	35.2082
31	4.6848	0.016	0.3719	3.2213	-0.7631	3.8307	-0.1161	-0.1606	-0.5388	0.0207	-0.1532	-0.7867	0.1986	0.5521	0.1091	0.2919	-0.0061	0.0135	0.4811	-0.0483	0.628	122.2071	50.5475	39.0684
32	0.4148	0.9216	0.4781	8.5282	0.315	0.9158	-0.0404	0.283	0.7189	0.1922	0.0634	0.73	-0.5771	-0.1688	-0.2504	0.3918	0.0261	0.0729	0.3571	0.0359	0.7609	22.0476	40.7106	69.9423
33	5.5135	0.2328	-0.8796	2.5883	0.9552	1.1854	0.1314	-0.1995	0.5918	0.1527	-0.1235	0.4149	-0.037	0.0113	0.0142	0.3861	-0.0212	0.0607	0.3026	-0.0527	0.457	31.8029	37.376	70.3601
34	3.3061	0.2478	-0.3117	4.0766	-1.1902	3.235	0.1695	-0.3838	0.3703	0.6039	-0.0276	0.5272	-0.3687	-0.0205	-0.1259	0.4024	-0.048	0.0845	0.3462	-0.1245	0.5802	38.6195	40.425	75.9513
35	5.2358	-0.3472	1.8874	1.0549	1.5482	3.3828	-0.0561	0.1137	0.212	0.1139	-0.056	0.0525	0.1002	0.2205	0.0945	0.275	-0.0306	0.0674	0.1692	0.0035	0.3498	43.5541	29.2585	67.5407
36	8.1208	-4.0558	0.2776	8.4866	-0.0377	8.0841	0.587	0.1895	-1.0942	-0.5693	-0.6322	-0.5932	0.7744	0.9947	0.0042	0.4188	0.0188	0.1048	0.4572	0.0712	0.6857	60.6856	20.876	52.9411
37	4.6808	1.7473	-0.5701	2.2406	0.2061	2.5045	-0.1172	0.2951	-0.0083	-0.0485	0.1878	-0.3748	-0.0436	0.2351	-0.0133	0.304	-0.0474	0.0439	0.2518	0.0333	0.4071	43.299	25.0775	62.2356
38	3.1166	-0.0528	1.5503	0.2293	-0.4965	3.4317	0.0308	-0.146	-0.1154	0.3181	-0.2394	0.0417	0.2077	0.1936	0.0109	0.388	-0.0167	0.1053	0.2389	-0.0218	0.4518	28.8976	23.4283	73.2743
39	0.3923	-0.5317	-0.0771	0.7332	0.1184	0.0303	-0.1288	-0.0998	0.39	0.6004	-0.1323	-0.404	0.0314	0.07	0.1984	0.4817	0.0003	-0.0312	0.4459	-0.0579	0.5222	60.7592	29.3978	67.1767
40	5.7951	0.0933	1.496	1.9222	0.2537	7.5407	-0.0916	0.3825	0.1801	-0.0808	-0.1959	-0.0046	0.1297	0.0106	0.1424	0.2766	-0.0558	0.0372	0.3001	0.0455	0.403	54.1447	34.8307	52.9169
41	0.1647	-1.0473	0.396	7.2898	-1.3167	4.1738	-0.5916	1.9226	0.6153	-0.865	0.5334	-0.2269	-0.2498	0.4229	0.0112	0.4221	-0.1701	-0.0757	0.8032	0.22	0.4864	51.7284	36.2269	42.5885
42	4.6021	0.4597	-0.023	2.6788	1.0376	5.6211	-0.5446	0.1189	-0.2763	-0.2158	-0.1411	0.0485	-0.0492	-0.0174	0.6562	0.3128	-0.076	0.0307	0.3224	-0.0065	0.4034	60.66	28.9443	50.635
43	3.5185	1.1173	0.6531	2.9723	4.6001	8.2503	-0.3042	0.4407	-0.131	-0.4304	-0.1079	0.4509	0.29	-0.7255	0.5619	0.3638	-0.0619	-0.0126	0.4144	0.0581	0.3948	63.517	34.4013	44.1322
44	6.2208	-4.2173	-4.4262	9.831	1.4478	3.495	0.0716	-1.1124	2.3093	0.7144	0.5337	-0.8961	-0.8891	0.8766	-0.4056	0.4683	-0.0991	0.0478	0.6575	-0.1	0.6701	65.5019	42.0139	57.5617
45	7.1654	2.387	-4.4388	4.0283	-1.944	6.6454	0.5151	-0.3109	2.2468	0.2274	0.4529	-0.4239	-1.0352	0.2395	-1.3341	0.569	0.0201	0.1971	0.4908	-0.0128	0.776	53.559	44.8093	58.2653
46	7.3124	0.2133	-5.4203	1.899	-0.5644	7.3728	0.3409	0.699	0.8259	-0.5309	0.4648	-0.2744	0.0657	-0.3246	-0.7686	0.3325	-0.0804	0.0337	0.3094	0.0061	0.3666	64.9051	37.2889	39.758

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 5303 through 5335 )
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 5336 through 5418 )
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 5419 through 5462 )
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 5463 through 5478 )
5	X-RAY DIFFRACTION	5	chain 'A' and (resid 5479 through 5505 )
6	X-RAY DIFFRACTION	6	chain 'A' and (resid 5506 through 5518 )
7	X-RAY DIFFRACTION	7	chain 'A' and (resid 5519 through 5540 )
8	X-RAY DIFFRACTION	8	chain 'A' and (resid 5541 through 5571 )
9	X-RAY DIFFRACTION	9	chain 'A' and (resid 5572 through 5591 )
10	X-RAY DIFFRACTION	10	chain 'B' and (resid 11 through 32 )
11	X-RAY DIFFRACTION	11	chain 'B' and (resid 33 through 72 )
12	X-RAY DIFFRACTION	12	chain 'B' and (resid 73 through 100 )
13	X-RAY DIFFRACTION	13	chain 'C' and (resid 5302 through 5417 )
14	X-RAY DIFFRACTION	14	chain 'C' and (resid 5418 through 5505 )
15	X-RAY DIFFRACTION	15	chain 'C' and (resid 5506 through 5591 )
16	X-RAY DIFFRACTION	16	chain 'D' and (resid 12 through 22 )
17	X-RAY DIFFRACTION	17	chain 'D' and (resid 23 through 32 )
18	X-RAY DIFFRACTION	18	chain 'D' and (resid 33 through 63 )
19	X-RAY DIFFRACTION	19	chain 'D' and (resid 64 through 79 )
20	X-RAY DIFFRACTION	20	chain 'D' and (resid 80 through 95 )
21	X-RAY DIFFRACTION	21	chain 'E' and (resid 5302 through 5335 )
22	X-RAY DIFFRACTION	22	chain 'E' and (resid 5336 through 5477 )
23	X-RAY DIFFRACTION	23	chain 'E' and (resid 5478 through 5505 )
24	X-RAY DIFFRACTION	24	chain 'E' and (resid 5506 through 5518 )
25	X-RAY DIFFRACTION	25	chain 'E' and (resid 5519 through 5540 )
26	X-RAY DIFFRACTION	26	chain 'E' and (resid 5541 through 5591 )
27	X-RAY DIFFRACTION	27	chain 'F' and (resid 12 through 22 )
28	X-RAY DIFFRACTION	28	chain 'F' and (resid 23 through 52 )
29	X-RAY DIFFRACTION	29	chain 'F' and (resid 53 through 63 )
30	X-RAY DIFFRACTION	30	chain 'F' and (resid 64 through 79 )
31	X-RAY DIFFRACTION	31	chain 'F' and (resid 80 through 95 )
32	X-RAY DIFFRACTION	32	chain 'G' and (resid 5303 through 5335 )
33	X-RAY DIFFRACTION	33	chain 'G' and (resid 5336 through 5418 )
34	X-RAY DIFFRACTION	34	chain 'G' and (resid 5419 through 5505 )
35	X-RAY DIFFRACTION	35	chain 'G' and (resid 5506 through 5540 )
36	X-RAY DIFFRACTION	36	chain 'G' and (resid 5541 through 5552 )
37	X-RAY DIFFRACTION	37	chain 'G' and (resid 5553 through 5571 )
38	X-RAY DIFFRACTION	38	chain 'G' and (resid 5572 through 5592 )
39	X-RAY DIFFRACTION	39	chain 'H' and (resid 10 through 15 )
40	X-RAY DIFFRACTION	40	chain 'H' and (resid 16 through 22 )
41	X-RAY DIFFRACTION	41	chain 'H' and (resid 23 through 32 )
42	X-RAY DIFFRACTION	42	chain 'H' and (resid 33 through 52 )
43	X-RAY DIFFRACTION	43	chain 'H' and (resid 53 through 72 )
44	X-RAY DIFFRACTION	44	chain 'H' and (resid 73 through 79 )
45	X-RAY DIFFRACTION	45	chain 'H' and (resid 80 through 87 )
46	X-RAY DIFFRACTION	46	chain 'H' and (resid 88 through 99 )

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