Details: 20 mM Na-Phosphate buffer pH 7.5, 300 mM NaCl, 1 mM DTT
Grid
Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 200 sec. / Pretreatment - Atmosphere: AIR / Pretreatment - Pressure: 0.039 kPa Details: Grids were glow discharged twice at 15 mA, 0.39 mBar, for 100 seconds each time
Vitrification
Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV Details: Sample volume 3 ul Wait time 5 s, Blot time 5 s, Delay time 0 s Force 12.
Details
Sample frozen directly after S200 size exclusion column
-
Electron microscopy
Microscope
JEOL CRYO ARM 200
Specialist optics
Energy filter - Name: In-column Omega Filter / Energy filter - Slit width: 20 eV
Image recording
Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Digitization - Dimensions - Width: 3838 pixel / Digitization - Dimensions - Height: 3710 pixel / Digitization - Frames/image: 1-40 / Number grids imaged: 1 / Number real images: 6579 / Average exposure time: 6.5 sec. / Average electron dose: 40.0 e/Å2
Electron beam
Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
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