EMDB-18765: Human 60S ribosomal subunit

Basic information

Entry

Database: EMDB / ID: EMD-18765

• Title: Human 60S ribosomal subunit
• Map data: Cryo-EM reconstruction of the HeLa large ribosomal subunit.

Sample

• Complex: Human mature large ribosomal subunit
  • Protein or peptide: x 41 types
  • RNA: x 3 types
• Ligand: x 8 types

• Keywords: Large ribosomal subunit / HeLa / modifications / polyamines / RIBOSOME

Function / homology

Function:

eukaryotic 80S initiation complex / negative regulation of protein neddylation / negative regulation of formation of translation preinitiation complex / regulation of G1 to G0 transition / axial mesoderm development / ribosomal protein import into nucleus / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / regulation of translation involved in cellular response to UV / protein-DNA complex disassembly / 90S preribosome assembly / positive regulation of DNA damage response, signal transduction by p53 class mediator / GAIT complex / A band / TORC2 complex binding / alpha-beta T cell differentiation / G1 to G0 transition / middle ear morphogenesis / exit from mitosis / translation at presynapse / optic nerve development / cytoplasmic side of rough endoplasmic reticulum membrane / retinal ganglion cell axon guidance / negative regulation of ubiquitin protein ligase activity / homeostatic process / response to aldosterone / macrophage chemotaxis / lung morphogenesis / male meiosis I / Protein hydroxylation / Peptide chain elongation / Selenocysteine synthesis / positive regulation of signal transduction by p53 class mediator / Formation of a pool of free 40S subunits / Eukaryotic Translation Termination / ubiquitin ligase inhibitor activity / Response of EIF2AK4 (GCN2) to amino acid deficiency / SRP-dependent cotranslational protein targeting to membrane / blastocyst development / cellular response to actinomycin D / negative regulation of ubiquitin-dependent protein catabolic process / Viral mRNA Translation / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / protein localization to nucleus / GTP hydrolysis and joining of the 60S ribosomal subunit / L13a-mediated translational silencing of Ceruloplasmin expression / Major pathway of rRNA processing in the nucleolus and cytosol / protein targeting / protein-RNA complex assembly / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / maturation of LSU-rRNA / rough endoplasmic reticulum / Maturation of protein E / negative regulation of proteasomal ubiquitin-dependent protein catabolic process / Maturation of protein E / MDM2/MDM4 family protein binding / cytosolic ribosome / ER Quality Control Compartment (ERQC) / Myoclonic epilepsy of Lafora / FLT3 signaling by CBL mutants / Prevention of phagosomal-lysosomal fusion / IRAK2 mediated activation of TAK1 complex / Alpha-protein kinase 1 signaling pathway / Glycogen synthesis / embryo implantation / IRAK1 recruits IKK complex / IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation / Membrane binding and targetting of GAG proteins / Endosomal Sorting Complex Required For Transport (ESCRT) / Regulation of TBK1, IKKε (IKBKE)-mediated activation of IRF3, IRF7 / Negative regulation of FLT3 / PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1 / Constitutive Signaling by NOTCH1 HD Domain Mutants / Regulation of TBK1, IKKε-mediated activation of IRF3, IRF7 upon TLR3 ligation / IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation / NOTCH2 Activation and Transmission of Signal to the Nucleus / TICAM1,TRAF6-dependent induction of TAK1 complex / TICAM1-dependent activation of IRF3/IRF7 / APC/C:Cdc20 mediated degradation of Cyclin B / Regulation of FZD by ubiquitination / Downregulation of ERBB4 signaling / p75NTR recruits signalling complexes / APC-Cdc20 mediated degradation of Nek2A / InlA-mediated entry of Listeria monocytogenes into host cells / TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling / Regulation of innate immune responses to cytosolic DNA / TRAF6-mediated induction of TAK1 complex within TLR4 complex / Regulation of pyruvate metabolism / cellular response to interleukin-4 / NF-kB is activated and signals survival / Downregulation of ERBB2:ERBB3 signaling / ossification / innate immune response in mucosa / Pexophagy / NRIF signals cell death from the nucleus / DNA damage response, signal transduction by p53 class mediator / Regulation of PTEN localization / VLDLR internalisation and degradation / Activated NOTCH1 Transmits Signal to the Nucleus / Synthesis of active ubiquitin: roles of E1 and E2 enzymes / Regulation of BACH1 activity

Domain/homology:

Ribosomal protein L6, N-terminal / Ribosomal protein L6, N-terminal domain / Ribosomal protein L30e / Ribosomal protein L2, archaeal-type / Ribosomal L15/L27a, N-terminal / Ribosomal protein L28e / Ribosomal protein L23 / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / metallochaperone-like domain / TRASH domain / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L10e / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / Ribosomal protein L44e signature. / : / Ribosomal protein L24e, conserved site / Ribosomal protein L24e signature. / : / Ribosomal protein L19, eukaryotic / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19e signature. / Ribosomal protein L6e signature. / Ribosomal protein L13e / Ribosomal protein L13e / 60S ribosomal protein L18a/ L20, eukaryotes / : / Ribosomal protein L44e / Ribosomal protein L44 / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L30e signature 1. / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal L40e family / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein 60S L18 and 50S L18e / Ribosomal Protein L6, KOW domain / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / Ribosomal protein L30e signature 2. / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal L27e protein family / Ribosomal protein L36e signature. / Ribosomal protein L35Ae, conserved site / Ribosomal protein L30e, conserved site / Ribosomal protein L35Ae signature. / Ribosomal protein L39e, conserved site / Ribosomal protein L39e signature. / : / Ribosomal protein L6e / Ribosomal protein L34Ae / Ribosomal protein L34e / 60S ribosomal protein L19 / Ribosomal protein L30/YlxQ / Ribosomal protein L7A/L8 / 60S ribosomal protein L6E / 60S ribosomal protein L35 / Ribosomal protein L13, eukaryotic/archaeal / Ribosomal protein L18e / 60S ribosomal protein L4, C-terminal domain / 60S ribosomal protein L4 C-terminal domain / Ribosomal protein L7, eukaryotic / Ribosomal protein L30, N-terminal / Ribosomal protein L31e, conserved site / Ribosomal L30 N-terminal domain / Ribosomal protein L31e signature. / Ribosomal protein L37ae / Ribosomal L37ae protein family / Ribosomal protein L36e / Ribosomal protein L36e domain superfamily / Ribosomal protein L36e / Ribosomal_L19e / Ribosomal protein L19/L19e / Ribosomal protein L19/L19e, domain 1 / Ribosomal protein L19/L19e superfamily / Ribosomal protein L19e, N-terminal domain / Ribosomal protein L14e domain / Ribosomal protein L14

Component:

Large ribosomal subunit protein eL33 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein eL13 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein eL22 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein eL21 / Large ribosomal subunit protein eL28 / Large ribosomal subunit protein eL29 / Large ribosomal subunit protein eL34 / Large ribosomal subunit protein eL14 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein eL15 / Large ribosomal subunit protein eL27 / Large ribosomal subunit protein eL43 / Large ribosomal subunit protein eL37 / Large ribosomal subunit protein eL8 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein eL31 / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein uL2 / Ubiquitin-ribosomal protein eL40 fusion protein / Large ribosomal subunit protein eL38 / Large ribosomal subunit protein eL24 / Large ribosomal subunit protein eL42 / Large ribosomal subunit protein eL19 / Large ribosomal subunit protein eL20 / Large ribosomal subunit protein eL6 / Large ribosomal subunit protein eL18 / Ribosomal protein uL16-like / Large ribosomal subunit protein eL36

• Biological species: Homo sapiens (human)
• Method: single particle reconstruction / cryo EM / Resolution: 1.78 Å
• Authors: Wiechert F / Schacherl M / Sprink T

Funding support

Germany, 1 items

Organization	Grant number	Country
German Research Foundation (DFG)		Germany

• Citation: Journal: Nucleic Acids Res / Year: 2025; Title: Visualizing the modification landscape of the human 60S ribosomal subunit at close to atomic resolution.; Authors: Franziska Wiechert / Anett Unbehaun / Thiemo Sprink / Helena Seibel / Jörg Bürger / Justus Loerke / Thorsten Mielke / Christoph A Diebolder / Magdalena Schacherl / Christian M T Spahn / ; Abstract: Chemical modifications of ribosomal RNAs (rRNAs) and proteins expand their topological repertoire, and together with the plethora of bound ligands, fine-tune ribosomal function. Detailed knowledge of this natural composition provides important insights into ribosome genesis and function and clarifies some aspects of ribosomopathies. The discovery of new structural properties and functional aspects of ribosomes has gone hand in hand with cryo-electron microscopy (cryo-EM) and its technological development. In line with the ability to visualize atomic details - a prerequisite for identifying chemical modifications and ligands in cryo-EM maps - in this work we present the structure of the 60S ribosomal subunit from HeLa cells at the very high global resolution of 1.78 Å. We identified 113 rRNA modifications and four protein modifications including uL2-Hisβ-ox216, which stabilizes the local structure near the peptidyl transferase centre via an extended hydrogen-bonding network. We can differentiate metal ions Mg2+ and K+, polyamines spermine, spermidine and putrescine and identify thousands of water molecules binding to the 60S subunit. Approaching atomic resolution cryo-EM has become a powerful tool to examine fine details of macromolecular structures that will expand our knowledge about translation and other biological processes in the future and assess the variability of the chemical space due to differences between species/tissues or varying physicochemical environment.

History

Deposition	Oct 26, 2023	-
Header (metadata) release	Dec 11, 2024	-
Map release	Dec 11, 2024	-
Update	Jan 22, 2025	-
Current status	Jan 22, 2025	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_18765.map.gz / Format: CCP4 / Size: 1000 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Cryo-EM reconstruction of the HeLa large ribosomal subunit.
• Voxel size: X=Y=Z: 0.616 Å

Density

Contour Level	By AUTHOR: 2.86
Minimum - Maximum	-17.531147000000001 - 48.267560000000003
Average (Standard dev.)	-0.00000000565372 (±1.0)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 640 640 640 Spacing 640 640 640
Cell	A=B=C: 394.24 Å α=β=γ: 90.0 °

Supplemental data

Mask #1

• File: emd_18765_msk_1.map

Half map: Cryo-EM reconstruction of the HeLa large ribosomal subunit....

• File: emd_18765_half_map_1.map
• Annotation: Cryo-EM reconstruction of the HeLa large ribosomal subunit. (Half map B)

Half map: Cryo-EM reconstruction of the HeLa large ribosomal subunit....

• File: emd_18765_half_map_2.map
• Annotation: Cryo-EM reconstruction of the HeLa large ribosomal subunit. (Half map A)

Sample components

Entire : Human mature large ribosomal subunit

• Entire: Name: Human mature large ribosomal subunit

Components

• Complex: Human mature large ribosomal subunit
  • Protein or peptide: Large ribosomal subunit protein uL30
  • Protein or peptide: 60S ribosomal protein L7a
  • Protein or peptide: 60S ribosomal protein L9
  • Protein or peptide: Ribosomal protein uL16-like
  • Protein or peptide: 60S ribosomal protein L11
  • Protein or peptide: 60S ribosomal protein L13
  • Protein or peptide: 60S ribosomal protein L14
  • Protein or peptide: 60S ribosomal protein L15
  • Protein or peptide: 60S ribosomal protein L13a
  • Protein or peptide: 60S ribosomal protein L17
  • Protein or peptide: 60S ribosomal protein L18
  • Protein or peptide: 60S ribosomal protein L19
  • Protein or peptide: 60S ribosomal protein L18a
  • Protein or peptide: 60S ribosomal protein L21
  • Protein or peptide: 60S ribosomal protein L22
  • Protein or peptide: 60S ribosomal protein L23
  • Protein or peptide: 60S ribosomal protein L24
  • Protein or peptide: 60S ribosomal protein L23a
  • Protein or peptide: 60S ribosomal protein L26
  • Protein or peptide: 60S ribosomal protein L27
  • Protein or peptide: Large ribosomal subunit protein uL15
  • Protein or peptide: 60S ribosomal protein L29
  • Protein or peptide: 60S ribosomal protein L30
  • Protein or peptide: 60S ribosomal protein L31
  • Protein or peptide: 60S ribosomal protein L32
  • Protein or peptide: 60S ribosomal protein L35a
  • Protein or peptide: 60S ribosomal protein L34
  • Protein or peptide: 60S ribosomal protein L35
  • Protein or peptide: 60S ribosomal protein L36
  • Protein or peptide: 60S ribosomal protein L37
  • Protein or peptide: 60S ribosomal protein L38
  • Protein or peptide: 60S ribosomal protein L39
  • Protein or peptide: Large ribosomal subunit protein eL40
  • Protein or peptide: 60S ribosomal protein L36a
  • Protein or peptide: 60S ribosomal protein L37a
  • Protein or peptide: 60S ribosomal protein L28
  • RNA: 28S rRNA
  • RNA: 5S rRNA
  • RNA: 5.8S rRNA
  • Protein or peptide: Large ribosomal subunit protein uL2
  • Protein or peptide: Large ribosomal subunit protein uL3
  • Protein or peptide: 60S ribosomal protein L4
  • Protein or peptide: 60S ribosomal protein L5
  • Protein or peptide: 60S ribosomal protein L6
• Ligand: MAGNESIUM ION
• Ligand: 1,4-DIAMINOBUTANE
• Ligand: POTASSIUM ION
• Ligand: ZINC ION
• Ligand: SPERMIDINE
• Ligand: SPERMINE
• Ligand: ADENOSINE-5'-TRIPHOSPHATE
• Ligand: water

Supramolecule #1: Human mature large ribosomal subunit

• Supramolecule: Name: Human mature large ribosomal subunit / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#44
• Source (natural): Organism: Homo sapiens (human)

Macromolecule #1: Large ribosomal subunit protein uL30

• Macromolecule: Name: Large ribosomal subunit protein uL30 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 29.290973 KDa

Sequence

String:

MEGVEEKKKE VPAVPETLKK KRRNFAELKI KRLRKKFAQK MLRKARRKLI YEKAKHYHKE YRQMYRTEIR MARMARKAGN FYVPAEPKL AFVIRIRGIN GVSPKVRKVL QLLRLRQIFN GTFVKLNKAS INMLRIVEPY IAWGYPNLKS VNELIYKRGY G KINKKRIA LTDNALIARS LGKYGIICME DLIHEIYTVG KRFKEANNFL WPFKLSSPRG GMKKKTTHFV EGGDAGNRED QI NRLIRRM N

UniProtKB: Large ribosomal subunit protein uL30

Macromolecule #2: 60S ribosomal protein L7a

• Macromolecule: Name: 60S ribosomal protein L7a / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 30.061785 KDa

Sequence

String:

MPKGKKAKGK KVAPAPAVVK KQEAKKVVNP LFEKRPKNFG IGQDIQPKRD LTRFVKWPRY IRLQRQRAIL YKRLKVPPAI NQFTQALDR QTATQLLKLA HKYRPETKQE KKQRLLARAE KKAAGKGDVP TKRPPVLRAG VNTVTTLVEN KKAQLVVIAH D VDPIELVV FLPALCRKMG VPYCIIKGKA RLGRLVHRKT CTTVAFTQVN SEDKGALAKL VEAIRTNYND RYDEIRRHWG GN VLGPKSV ARIAKLEKAK AKELATKLG

UniProtKB: Large ribosomal subunit protein eL8

Macromolecule #3: 60S ribosomal protein L9

• Macromolecule: Name: 60S ribosomal protein L9 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 21.899471 KDa

Sequence

String:

MKTILSNQTV DIPENVDITL KGRTVIVKGP RGTLRRDFNH INVELSLLGK KKKRLRVDKW WGNRKELATV RTICSHVQNM IKGVTLGFR YKMRSVYAHF PINVVIQENG SLVEIRNFLG EKYIRRVRMR PGVACSVSQA QKDELILEGN DIELVSNSAA L IQQATTVK NKDIRKFLDG IYVSEKGTVQ QADE

UniProtKB: Large ribosomal subunit protein uL6

Macromolecule #4: Ribosomal protein uL16-like

• Macromolecule: Name: Ribosomal protein uL16-like / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 24.570949 KDa

Sequence

String:

MGRRPARCYR YCKNKPYPKS RFCRGVPDAK IRIFDLGRKK AKVDEFPLGG HMVSDEYEQL SSEALEAARI CANKYMVKSC GRDGFHMRV RLHPFHVIRI NKMLSCAGAD RLQTGMRGAF GKPQGTVARV HIGQVIMSIR TKLQNEEHVI EALRRAKFKF P GRQKIHIS KKWGFTKFNA DEFEDMVAKK CLIPDGCGVK YVPSHGPLDK WRVLHS

UniProtKB: Ribosomal protein uL16-like

Macromolecule #5: 60S ribosomal protein L11

• Macromolecule: Name: 60S ribosomal protein L11 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 20.288465 KDa

Sequence

String:

MAQDQGEKEN PMRELRIRKL CLNICVGESG DRLTRAAKVL EQLTGQTPVF SKARYTVRSF GIRRNEKIAV HCTVRGAKAE EILEKGLKV REYELRKNNF SDTGNFGFGI QEHIDLGIKY DPSIGIYGLD FYVVLGRPGF SIADKKRRTG CIGAKHRISK E EAMRWFQQ KYDGIILPGK

UniProtKB: Large ribosomal subunit protein uL5

Macromolecule #6: 60S ribosomal protein L13

• Macromolecule: Name: 60S ribosomal protein L13 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 24.321682 KDa

Sequence

String:

MAPSRNGMVL KPHFHKDWQR RVATWFNQPA RKIRRRKARQ AKARRIAPRP ASGPIRPIVR CPTVRYHTKV RAGRGFSLEE LRVAGIHKK VARTIGISVD PRRRNKSTES LQANVQRLKE YRSKLILFPR KPSAPKKGDS SAEELKLATQ LTGPVMPVRN V YKKEKARV ITEEEKNFKA FASLRMARAN ARLFGIRAKR AKEAAEQDVE KKK

UniProtKB: Large ribosomal subunit protein eL13

Macromolecule #7: 60S ribosomal protein L14

• Macromolecule: Name: 60S ribosomal protein L14 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 23.485016 KDa

Sequence

String:

MVFRRFVEVG RVAYVSFGPH AGKLVAIVDV IDQNRALVDG PCTQVRRQAM PFKCMQLTDF ILKFPHSAHQ KYVRQAWQKA DINTKWAAT RWAKKIEARE RKAKMTDFDR FKVMKAKKMR NRIIKNEVKK LQKAALLKAS PKKAPGTKGT AAAAAAAAAA K VPAKKITA ASKKAPAQKV PAQKATGQKA APAPKAQKGQ KAPAQKAPAP KASGKKA

UniProtKB: Large ribosomal subunit protein eL14

Macromolecule #8: 60S ribosomal protein L15

• Macromolecule: Name: 60S ribosomal protein L15 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 24.207285 KDa

Sequence

String:

MGAYKYIQEL WRKKQSDVMR FLLRVRCWQY RQLSALHRAP RPTRPDKARR LGYKAKQGYV IYRIRVRRGG RKRPVPKGAT YGKPVHHGV NQLKFARSLQ SVAEERAGRH CGALRVLNSY WVGEDSTYKF FEVILIDPFH KAIRRNPDTQ WITKPVHKHR E MRGLTSAG RKSRGLGKGH KFHHTIGGSR RAAWRRRNTL QLHRYR

UniProtKB: Large ribosomal subunit protein eL15

Macromolecule #9: 60S ribosomal protein L13a

• Macromolecule: Name: 60S ribosomal protein L13a / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 23.633412 KDa

Sequence

String:

MAEVQVLVLD GRGHLLGRLA AIVAKQVLLG RKVVVVRCEG INISGNFYRN KLKYLAFLRK RMNTNPSRGP YHFRAPSRIF WRTVRGMLP HKTKRGQAAL DRLKVFDGIP PPYDKKKRMV VPAALKVVRL KPTRKFAYLG RLAHEVGWKY QAVTATLEEK R KEKAKIHY RKKKQLMRLR KQAEKNVEKK IDKYTEVLKT HGLLV

UniProtKB: Large ribosomal subunit protein uL13

Macromolecule #10: 60S ribosomal protein L17

• Macromolecule: Name: 60S ribosomal protein L17 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 21.44317 KDa

Sequence

String:

MVRYSLDPEN PTKSCKSRGS NLRVHFKNTR ETAQAIKGMH IRKATKYLKD VTLQKQCVPF RRYNGGVGRC AQAKQWGWTQ GRWPKKSAE FLLHMLKNAE SNAELKGLDV DSLVIEHIQV NKAPKMRRRT YRAHGRINPY MSSPCHIEMI LTEKEQIVPK P EEEVAQKK KISQKKLKKQ KLMARE

UniProtKB: Large ribosomal subunit protein uL22

Macromolecule #11: 60S ribosomal protein L18

• Macromolecule: Name: 60S ribosomal protein L18 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 21.687676 KDa

Sequence

String:

MGVDIRHNKD RKVRRKEPKS QDIYLRLLVK LYRFLARRTN STFNQVVLKR LFMSRTNRPP LSLSRMIRKM KLPGRENKTA VVVGTITDD VRVQEVPKLK VCALRVTSRA RSRILRAGGK ILTFDQLALD SPKGCGTVLL SGPRKGREVY RHFGKAPGTP H SHTKPYVR SKGRKFERAR GRRASRGYKN

UniProtKB: Large ribosomal subunit protein eL18

Macromolecule #12: 60S ribosomal protein L19

• Macromolecule: Name: 60S ribosomal protein L19 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 23.535281 KDa

Sequence

String:

MSMLRLQKRL ASSVLRCGKK KVWLDPNETN EIANANSRQQ IRKLIKDGLI IRKPVTVHSR ARCRKNTLAR RKGRHMGIGK RKGTANARM PEKVTWMRRM RILRRLLRRY RESKKIDRHM YHSLYLKVKG NVFKNKRILM EHIHKLKADK ARKKLLADQA E ARRSKTKE ARKRREERLQ AKKEEIIKTL SKEEETKK

UniProtKB: Large ribosomal subunit protein eL19

Macromolecule #13: 60S ribosomal protein L18a

• Macromolecule: Name: 60S ribosomal protein L18a / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 20.808514 KDa

Sequence

String:

MKASGTLREY KVVGRCLPTP KCHTPPLYRM RIFAPNHVVA KSRFWYFVSQ LKKMKKSSGE IVYCGQVFEK SPLRVKNFGI WLRYDSRSG THNMYREYRD LTTAGAVTQC YRDMGARHRA RAHSIQIMKV EEIAASKCRR PAVKQFHDSK IKFPLPHRVL R RQHKPRFT TKRPNTFF

UniProtKB: Large ribosomal subunit protein eL20

Macromolecule #14: 60S ribosomal protein L21

• Macromolecule: Name: 60S ribosomal protein L21 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 18.609988 KDa

Sequence

String:

MTNTKGKRRG TRYMFSRPFR KHGVVPLATY MRIYKKGDIV DIKGMGTVQK GMPHKCYHGK TGRVYNVTQH AVGIVVNKQV KGKILAKRI NVRIEHIKHS KSRDSFLKRV KENDQKKKEA KEKGTWVQLK RQPAPPREAH FVRTNGKEPE LLEPIPYEFM A

UniProtKB: Large ribosomal subunit protein eL21

Macromolecule #15: 60S ribosomal protein L22

• Macromolecule: Name: 60S ribosomal protein L22 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.813015 KDa

Sequence

String:

MAPVKKLVVK GGKKKKQVLK FTLDCTHPVE DGIMDAANFE QFLQERIKVN GKAGNLGGGV VTIERSKSKI TVTSEVPFSK RYLKYLTKK YLKKNNLRDW LRVVANSKES YELRYFQINQ DEEEEEDED

UniProtKB: Large ribosomal subunit protein eL22

Macromolecule #16: 60S ribosomal protein L23

• Macromolecule: Name: 60S ribosomal protein L23 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.892505 KDa

Sequence

String:

MSKRGRGGSS GAKFRISLGL PVGAVINCAD NTGAKNLYII SVKGIKGRLN RLPAAGVGDM VMATVKKGKP ELRKKVHPAV VIRQRKSYR RKDGVFLYFE DNAGVIVNNK GEMKGSAITG PVAKECADLW PRIASNAGSI A

UniProtKB: Large ribosomal subunit protein uL14

Macromolecule #17: 60S ribosomal protein L24

• Macromolecule: Name: 60S ribosomal protein L24 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.825111 KDa

Sequence

String:

MKVELCSFSG YKIYPGHGRR YARTDGKVFQ FLNAKCESAF LSKRNPRQIN WTVLYRRKHK KGQSEEIQKK RTRRAVKFQR AITGASLAD IMAKRNQKPE VRKAQREQAI RAAKEAKKAK QASKKTAMAA AKAPTKAAPK QKIVKPVKVS APRVGGKR

UniProtKB: Large ribosomal subunit protein eL24

Macromolecule #18: 60S ribosomal protein L23a

• Macromolecule: Name: 60S ribosomal protein L23a / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.740193 KDa

Sequence

String:

MAPKAKKEAP APPKAEAKAK ALKAKKAVLK GVHSHKKKKI RTSPTFRRPK TLRLRRQPKY PRKSAPRRNK LDHYAIIKFP LTTESAMKK IEDNNTLVFI VDVKANKHQI KQAVKKLYDI DVAKVNTLIR PDGEKKAYVR LAPDYDALDV ANKIGII

UniProtKB: Large ribosomal subunit protein uL23

Macromolecule #19: 60S ribosomal protein L26

• Macromolecule: Name: 60S ribosomal protein L26 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.303363 KDa

Sequence

String:

MKFNPFVTSD RSKNRKRHFN APSHIRRKIM SSPLSKELRQ KYNVRSMPIR KDDEVQVVRG HYKGQQIGKV VQVYRKKYVI YIERVQREK ANGTTVHVGI HPSKVVITRL KLDKDRKKIL ERKAKSRQVG KEKGKYKEET IEKMQE

UniProtKB: Large ribosomal subunit protein uL24

Macromolecule #20: 60S ribosomal protein L27

• Macromolecule: Name: 60S ribosomal protein L27 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.835831 KDa

Sequence

String:

MGKFMKPGKV VLVLAGRYSG RKAVIVKNID DGTSDRPYSH ALVAGIDRYP RKVTAAMGKK KIAKRSKIKS FVKVYNYNHL MPTRYSVDI PLDKTVVNKD VFRDPALKRK ARREAKVKFE ERYKTGKNKW FFQKLRF

UniProtKB: Large ribosomal subunit protein eL27

Macromolecule #21: Large ribosomal subunit protein uL15

• Macromolecule: Name: Large ribosomal subunit protein uL15 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 16.619527 KDa

Sequence

String:

MPSRLRKTRK LRGHVSHGHG RIGKHRKHPG GRGNAGGL(V5N)H HRINFDKYHP GYFGKVGMKH YHLKRNQSFC PTVNLD KLW TLVSEQTRVN AAKNKTGAAP IIDVVRSGYY KVLGKGKLPK QPVIVKAKFF SRRAEEKIKS VGGACVLVA

UniProtKB: Large ribosomal subunit protein uL15

Macromolecule #22: 60S ribosomal protein L29

• Macromolecule: Name: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.804275 KDa

Sequence

String:

MAKSKNHTTH NQSRKWHRNG IKKPRSQRYE SLKGVDPKFL RNMRFAKKHN KKGLKKMQAN NAKAMSARAE AIKALVKPKE VKPKIPKGV SRKLDRLAYI AHPKLGKRAR ARIAKGLRLC RPKAKAKAKA KDQTKAQAAA PASVPAQAPK RTQAPTKASE

UniProtKB: Large ribosomal subunit protein eL29

Macromolecule #23: 60S ribosomal protein L30

• Macromolecule: Name: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 12.805092 KDa

Sequence

String:

MVAAKKTKKS LESINSRLQL VMKSGKYVLG YKQTLKMIRQ GKAKLVILAN NCPALRKSEI EYYAMLAKTG VHHYSGNNIE LGTACGKYY RVCTLAIIDP GDSDIIRSMP EQTGEK

UniProtKB: Large ribosomal subunit protein eL30

Macromolecule #24: 60S ribosomal protein L31

• Macromolecule: Name: 60S ribosomal protein L31 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.494938 KDa

Sequence

String:

MAPAKKGGEK KKGRSAINEV VTREYTINIH KRIHGVGFKK RAPRALKEIR KFAMKEMGTP DVRIDTRLNK AVWAKGIRNV PYRIRVRLS RKRNEDEDSP NKLYTLVTYV PVTTFKNLQT VNVDEN

UniProtKB: Large ribosomal subunit protein eL31

Macromolecule #25: 60S ribosomal protein L32

• Macromolecule: Name: 60S ribosomal protein L32 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.898932 KDa

Sequence

String:

MAALRPLVKP KIVKKRTKKF IRHQSDRYVK IKRNWRKPRG IDNRVRRRFK GQILMPNIGY GSNKKTKHML PSGFRKFLVH NVKELEVLL MCNKSYCAEI AHNVSSKNRK AIVERAAQLA IRVTNPNARL RSEENE

UniProtKB: Large ribosomal subunit protein eL32

Macromolecule #26: 60S ribosomal protein L35a

• Macromolecule: Name: 60S ribosomal protein L35a / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 12.564743 KDa

Sequence

String:

MSGRLWSKAI FAGYKRGLRN QREHTALLKI EGVYARDETE FYLGKRCAYV YKAKNNTVTP GGKPNKTRVI WGKVTRAHGN SGMVRAKFR SNLPAKAIGH RIRVMLYPSR I

UniProtKB: Large ribosomal subunit protein eL33

Macromolecule #27: 60S ribosomal protein L34

• Macromolecule: Name: 60S ribosomal protein L34 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.326074 KDa

Sequence

String:

MVQRLTYRRR LSYNTASNKT RLSRTPGNRI VYLYTKKVGK APKSACGVCP GRLRGVRAVR PKVLMRLSKT KKHVSRAYGG SMCAKCVRD RIKRAFLIEE QKIVVKVLKA QAQSQKAK

UniProtKB: Large ribosomal subunit protein eL34

Macromolecule #28: 60S ribosomal protein L35

• Macromolecule: Name: 60S ribosomal protein L35 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.593624 KDa

Sequence

String:

MAKIKARDLR GKKKEELLKQ LDDLKVELSQ LRVAKVTGGA ASKLSKIRVV RKSIARVLTV INQTQKENLR KFYKGKKYKP LDLRPKKTR AMRRRLNKHE ENLKTKKQQR KERLYPLRKY AVKA

UniProtKB: Large ribosomal subunit protein uL29

Macromolecule #29: 60S ribosomal protein L36

• Macromolecule: Name: 60S ribosomal protein L36 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 12.290859 KDa

Sequence

String:

MALRYPMAVG LNKGHKVTKN VSKPRHSRRR GRLTKHTKFV RDMIREVCGF APYERRAMEL LKVSKDKRAL KFIKKRVGTH IRAKRKREE LSNVLAAMRK AAAKKD

UniProtKB: Large ribosomal subunit protein eL36

Macromolecule #30: 60S ribosomal protein L37

• Macromolecule: Name: 60S ribosomal protein L37 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 11.111032 KDa

Sequence

String:

MTKGTSSFGK RRNKTHTLCR RCGSKAYHLQ KSTCGKCGYP AKRKRKYNWS AKAKRRNTTG TGRMRHLKIV YRRFRHGFRE GTTPKPKRA AVAASSSS

UniProtKB: Large ribosomal subunit protein eL37

Macromolecule #31: 60S ribosomal protein L38

• Macromolecule: Name: 60S ribosomal protein L38 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 8.238948 KDa

Sequence

String:

MPRKIEEIKD FLLTARRKDA KSVKIKKNKD NVKFKVRCSR YLYTLVITDK EKAEKLKQSL PPGLAVKELK

UniProtKB: Large ribosomal subunit protein eL38

Macromolecule #32: 60S ribosomal protein L39

• Macromolecule: Name: 60S ribosomal protein L39 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 6.426759 KDa

Sequence

String:

MSSHKTFRIK RFLAKKQKQN RPIPQWIRMK TGNKIRYNSK RRHWRRTKLG L

UniProtKB: Large ribosomal subunit protein eL39

Macromolecule #33: Large ribosomal subunit protein eL40

• Macromolecule: Name: Large ribosomal subunit protein eL40 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.771411 KDa

Sequence

String:

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGIIEP SLRQLAQKY NCDKMICR(MLZ)C YARLHPRAVN CRKKKCGHTN NLRPKKKVK

UniProtKB: Ubiquitin-ribosomal protein eL40 fusion protein

Macromolecule #34: 60S ribosomal protein L36a

• Macromolecule: Name: 60S ribosomal protein L36a / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 12.476973 KDa

Sequence

String:

MVNVPKTRRT FCKKCGKHQP HKVTQYKKGK DSLYAQGKRR YDRKQSGYGG QTKPIFRKKA KTTKKIVLRL ECVEPNCRSK RMLAIKRCK HFELGGDKKR KGQVIQF

UniProtKB: Large ribosomal subunit protein eL42

Macromolecule #35: 60S ribosomal protein L37a

• Macromolecule: Name: 60S ribosomal protein L37a / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 10.29935 KDa

Sequence

String:

MAKRTKKVGI VGKYGTRYGA SLRKMVKKIE ISQHAKYTCS FCGKTKMKRR AVGIWHCGSC MKTVAGGAWT YNTTSAVTVK SAIRRLKEL KDQ

UniProtKB: Large ribosomal subunit protein eL43

Macromolecule #36: 60S ribosomal protein L28

• Macromolecule: Name: 60S ribosomal protein L28 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.784622 KDa

Sequence

String:

MSAHLQWMVV RNCSSFLIKR NKQTYSTEPN NLKARNSFRY NGLIHRKTVG VEPAADGKGV VVVIKRRSGQ RKPATSYVRT TINKNARAT LSSIRHMIRK NKYRPDLRMA AIRRASAILR SQKPVMVKRK RTRPTKSS

UniProtKB: Large ribosomal subunit protein eL28

Macromolecule #40: Large ribosomal subunit protein uL2

• Macromolecule: Name: Large ribosomal subunit protein uL2 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 28.103855 KDa

Sequence

String:

MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH DPGRGAPLAK VVFRDPYRFK KRTELFIAAE GIHTGQFVY CGKKAQLNIG NVLPVGTMPE GTIVCCLEEK PGDRGKLARA SGNYATVISH NPETKKTRVK LPSGSKKVIS S ANRAVVGV VAGGGRIDKP ILKAGRAYHK YKAKRNCWPR VRGVAMNPVE HPFGGGN(V5N)QH IGKPSTIRRD APAGRKVG L IAARRTGRLR GTKTVQEKEN

UniProtKB: Large ribosomal subunit protein uL2

Macromolecule #41: Large ribosomal subunit protein uL3

• Macromolecule: Name: Large ribosomal subunit protein uL3 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 46.224133 KDa

Sequence

String:

MSHRKFSAPR HGSLGFLPRK RSSRHRGKVK SFPKDDPSKP VHLTAFLGYK AGMTHIVREV DRPGSKVNKK EVVEAVTIVE TPPMVVVGI VGYVETPRGL RTFKTVFAEH ISDECKRRFY KNWHKSKKKA FTKYCKKWQD EDGKKQLEKD FSSMKKYCQV I RVIAHTQM RLLPLRQKKA HLMEIQVNGG TVAEKLDWAR ERLEQQVPVN QVFGQDEMID VIGVTKGKGY KGVTSRWHTK KL PRKT(HIC)RG LRKVACIGAW HPARVAFSVA RAGQKGYHHR TEINKKIYKI GQGYLIKDGK LIKNNASTDY DLSDKSINP LGGFVHYGEV TNDFVMLKGC VVGTKKRVLT LRKSLLVQTK RRALEKIDLK FIDTTSKFGH GRFQTMEEKK AFMGPLKKDR IAKEEGA

UniProtKB: Large ribosomal subunit protein uL3

Macromolecule #42: 60S ribosomal protein L4

• Macromolecule: Name: 60S ribosomal protein L4 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 47.804621 KDa

Sequence

String:

MACARPLISV YSEKGESSGK NVTLPAVFKA PIRPDIVNFV HTNLRKNNRQ PYAVSELAGH QTSAESWGTG RAVARIPRVR GGGTHRSGQ GAFGNMCRGG RMFAPTKTWR RWHRRVNTTQ KRYAICSALA ASALPALVMS KGHRIEEVPE LPLVVEDKVE G YKKTKEAV LLLKKLKAWN DIKKVYASQR MRAGKGKMRN RRRIQRRGPC IIYNEDNGII KAFRNIPGIT LLNVSKLNIL KL APGGHVG RFCIWTESAF RKLDELYGTW RKAASLKSNY NLPMHKMINT DLSRILKSPE IQRALRAPRK KIHRRVLKKN PLK NLRIML KLNPYAKTMR RNTILRQARN HKLRVDKAAA AAAALQAKSD EKAAVAGKKP VVGKKGKKAA VGVKKQKKPL VGKK AAATK KPAPEKKPAE KKPTTEEKKP AA

UniProtKB: Large ribosomal subunit protein uL4

Macromolecule #43: 60S ribosomal protein L5

• Macromolecule: Name: 60S ribosomal protein L5 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 34.426789 KDa

Sequence

String:

MGFVKVVKNK AYFKRYQVKF RRRREGKTDY YARKRLVIQD KNKYNTPKYR MIVRVTNRDI ICQIAYARIE GDMIVCAAYA HELPKYGVK VGLTNYAAAY CTGLLLARRL LNRFGMDKIY EGQVEVTGDE YNVESIDGQP GAFTCYLDAG LARTTTGNKV F GALKGAVD GGLSIPHSTK RFPGYDSESK EFNAEVHRKH IMGQNVADYM RYLMEEDEDA YKKQFSQYIK NSVTPDMMEE MY KKAHAAI RENPVYEKKP KKEVKKKRWN RPKMSLAQKK DRVAQKKASF LRAQERAAES

UniProtKB: Large ribosomal subunit protein uL18

Macromolecule #44: 60S ribosomal protein L6

• Macromolecule: Name: 60S ribosomal protein L6 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 32.810176 KDa

Sequence

String:

MAGEKVEKPD TKEKKPEAKK VDAGGKVKKG NLKAKKPKKG KPHCSRNPVL VRGIGRYSRS AMYSRKAMYK RKYSAAKSKV EKKKKEKVL ATVTKPVGGD KNGGTRVVKL RKMPRYYPTE DVPRKLLSHG KKPFSQHVRK LRASITPGTI LIILTGRHRG K RVVFLKQL ASGLLLVTGP LVLNRVPLRR THQKFVIATS TKIDISNVKI PKHLTDAYFK KKKLRKPRHQ EGEIFDTEKE KY EITEQRK IDQKAVDSQI LPKIKAIPQL QGYLRSVFAL TNGIYPHKLV F

UniProtKB: Large ribosomal subunit protein eL6

Macromolecule #37: 28S rRNA

• Macromolecule: Name: 28S rRNA / type: rna / ID: 37 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 1.640816625 MDa

Sequence

String:

CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAG AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC CCGCGGCGGG GCGCGGGACA UGUGGCGUAC G GAAGACCC GCUCCCCGGC GCCGCUCGUG GGGGGCCCAA GUCCUUCUGA UCGAGGCCCA GCCCG(PSU)GGAC GGUGUGAG G CCGGUAGCGG CCCCCGGCGC GCCGGGCCCG GGUCUUCCCG GAGUCGGGUU GCUUGGGAAU GCAGCCCAAA GCGGGUGGU AAACUCCAUC UAAGGCUAAA UACCGGCACG AGACCGAUAG UCAACAAGUA CCGUAAGGGA AAGUUGAAAA GAACUUUGAA G(A2M)G(A2M)GAGUU CAAGAGGGCG UGAAACCGUU AAGAGGUAAA CGGGUGGGGU CCGCGCAGUC CGCCCGGAGG AUU CAACCC GGCGGCGGGU CCGGCCGUGU CGGCGGCCCG GCGGAUCUUU CCCGCCCCCC GUUCCUCCCG ACCCCUCCAC CCGC CCUCC CUUCCCCCGC CGCCCCUCCU CCUCCUCCCC GGAGGGGGCG GGCUCCGGCG GGUGCGGGGG UGGGCGGGCG GGGCC GGGG GUGGGGUCGG CGGGGGACCG UCCCCCGACC GGCGACCGGC CGCCGCCGGG CGCAUUUCCA CCGCGGCGGU GCGCCG CGA CCGGCUCCGG GACGGCUGGG AAGGCCCGGC GGGGAAGGUG GCUCGGGGGG CCCCGUCCGU CCGUCCGUCC GUCCUCC UC CUCCCCCGUC UCCGCCCCCC GGCCCCGCGU CCUCCCUCGG GAGGGCGCGC GGGUCGGGGC GGCGGCGGCG GCGGCGGU G GCGGCGGCGG CGGCGGCGGC GGGACCGAAA CCCCCCCCGA GUGUUACAGC CCCCCCGGCA GCAGCACUCG CCGAAUCCC GGGGCCGAGG GAGCGAGACC CGUCGCCGCG CUCUCCCCCC UCCCGGCGCC CACCCCCGCG GGGAAUCCCC CGCGAGGGGG GUCUCCCCC GCGGGGGCGC GCCGGCGUCU CCUCGUGGGG GGGCCGGGCC ACCCCUCCCA CGGCGCGACC GCUCUCCCAC C CCUCCUCC CCGCGCCCCC GCCCCGGCGA CGGGGGGGGU GCCGCGCGCG GGUCGGGGGG CGGGGCGGAC UGUCCCCAGU GC GCCCCGG GCGGGUCGCG CCGUCGGGCC CGGGGGAGGU UCUCUCGGGG CCACGCGCGC GUCCCCCGAA GAGGGGGACG GCG GAGCGA GCGCACGGGG UCGGCGGCGA CGUCGGCUAC CCACCCGACC C(OMG)UCUUG(1MA)(A2M)A C(A2M)CGGACC A AGGAGU(OMC)UAA CACGUGCGCG AGUCGGGGGC UCGCACGAAA GCCGCCGUGG CGCAAUGAAG GUGAAGGCCG GCGCG CUCG CCGGCCGAGG UGGGAUCCCG AGGCCUCUCC AGUCCGCCGA GGGCGCACCA CCGGCCCGUC UCGCCCGCCG CGCCGG GGA GGUGGAGCAC GAGCGCACGU GUUAGGACCC (OMG)A(A2M)AGAUGGU GA(A2M)C(PSU)AUGCC UGGGCAGGGC G AAGCCAGA GGAAACUCUG GUGGAGGUCC GUAGCGGUCC UGACGUGCAA AUCGGUCGUC CGACCUGGGU AUAG(OMG)GGC G AAAGACUAAU CGAACCAUCU AGUAGCUGGU UCCCUCCGAA GUUUCCC(PSU)CA GGA(PSU)AGCUGG CGCUCUCGCA G ACCCGACG CACCCCCGCC ACGCAGUUUU AUCCGGUAAA GCGAA(PSU)GAUU AGAGGUCUUG GGGCCGAAAC GAUCUCAA C C(PSU)A(PSU)(PSU)CUCAA ACUU(PSU)AAAUG GGUAAGAAGC CCGGCUCGCU GGCGUGGAGC CGGGCGUGGA AUG CGAGUG CCUAGUGGGC CA(B8T)(PSU)U(PSU)UGGU AAGC(A2M)GAACU GGCG(OMC)UGCGG GAUGAACCGA ACGC CGGGU UAAGGCGCCC GAUGCCGACG CUCAUCAGAC CCCAGAAAAG GUGUUGGUUG AUAUAGACAG CAGGACGGUG GCCAU GGAA GUCGGAAUCC GCUAAGGAGU GUGUAACAAC UCACCUGCCG AAUCAACUAG CCCUGAAAAU GGAUGGCGCU GGAGCG UCG GGCCCAUACC CGGCCGUCGC CGGCAGUCGA GAGUGGACGG GAGCGGCGGG GGCGGCGCGC GCGCGCGCGC GUGUGGU GU GCGUCGGAGG GCGGCGGCGG CGGCGGCGGC GGGGGUGUGG GGUCCUUCCC CCGCCCCCCC CCCCACGCCU CCUCCCCU C CUCCCGCCCA CGCCCCGCUC CCCGCCCCCG GAGCCCCGCG GACGCUACGC CGCGACGAGU AGGAGGGCCG CUGCGGUGA GCCUUGAAGC CUAGGGCGCG GGCCCGGGUG GAGCCGCCGC AGGUGCAGAU (OMC)UUGGUGGUA GU(A2M)(OMG) (OMC)AAAU AUUCAAACGA GAACUUUGAA GGCCGAAGUG G(A2M)GAAGGGUU CCAUG(OMU)GAAC AG(OMC)A(OMG) UUGA ACAUGGGUCA GUCGGUCCUG AGAGAUGGGC GAGCGCCGUU CCGAAGGGAC GGGCGAUGGC CUCCGUUGCC CUCGGC CGA (PSU)CGAAAGGGA GUCGGGUUCA GAUCCCCGAA UCCGGAGUGG CGGAGAUGGG CGCCGCGAGG CGUCCAGUGC GGU AACGCG ACCGAUCCCG GAGAAGCCGG CGGGAGCCCC GGGGAGAGUU CUCUU(PSU)UCUU UGUGAAGGGC AGGGCGCCCU GGAAUGGGU UCGCCCCGAG AGAGGGGCCC GUGCCUUGGA AAGCGUCGCG GUUCCGGCGG CGUCCGGUGA GCUCUCGCUG G CCCUUGAA AAUCCGGGGG AGAGGGUGUA AAUCUCGCGC CGGGCCGUAC CC(A2M)UAUCCGC AGCAGGUCU(OMC) CAAG GUGAA C(A2M)GCCUCUGG (OMC)AUGUUGGAA CAA(OMU)G(PSU)AGGU AAGGGAAGUC GGCAAGC(OMC)GG AUCCG UAAC UUC(OMG)GGAUAA GGAUUGGCUC UAAGGGCUGG GUCGGUCGCG CUGGGGCGCG AAGCGGGGCU GGGCGCGCGC CG CGGCUGG ACGAGGCGCC GCCGCCCCCC CCACGCCCGG GGCACCCCCC UCGCGGCCCU CCCCCGCCCC ACCCCGCGCG CGC CGCUCG CUCCCUCCCC GCCCCGCGCC CUCUCUCUCU CUCUCUCCCC CGCUCCCCGU CCUCCCCCCU CCCCGGGGGA GCGC CGCGU GGGGGCGGCG GCGGGGGGAG AAGGGUCGGG GCGGCAGGGG CCGGCGGCGG CCCGCCGCGG GGCCCCGGCG GCGGG GGCA CGGUCCCCCG CGAGGGGGGC CCGGGCACCC GGGGGGCCGG CGGCGGCGGC GACUCUGGAC GCGAGCCGGG CCCUUC CCG UGGAUCGCCC CAGCUGCGGC GGGCGUCGCG GCCGCCCCCG GGGAGCCCGG CGGGCGCCGG CGCGCCCCCC CCCCCAC CC CACGUCUCGU CGCGCGCGCG UCCGCUGGGG GCGGGGAGCG GUCGGGCGGC GGCGGUCGGC GGGCGGCGGG GCGGGGCG G UUCGUCCCCC CGCCCUACCC CCCCGGCCCC GUCCGCCCCC CGUUCCCCCC UCCUCCUCGG CGCGCGGCGG CGGCGGCGG CAGGCGGCGG AGGGGCCGCG GGCCGGUCCC CCCCGCCGGG UCCGCCCCCG GGGCCGCGGU UCCGCGCGGC GCCUCGCCUC GGCCGGCGC CUAGCAGCCG ACUUAGAACU GGUGCGGACC AGG(OMG)GAAUCC GAC(PSU)G(PSU)UUAA UUAAAACAA AGCAUCGCGA AGGCCC(OMG)CGG CGGGUGUUGA CGCGAUGUGA UU(PSU)CUGCC(OMC)A GUGCUCUGAA UG(PSU)C A(A2M)AGU GAAGAAAUUC AAUGAAGCGC GG(OMG)UAAACGG CGGGAGUAAC UAUGACUCUC UUAAGGUAGC (5MC)AA (A2M)UGCCU C(OMG)UCAUCUAA UUAGUGA(OMC)GC GCAUGAA(UY1)GG A(PSU)GA(A2M)CGAG(A2M) UUCCCA CUG U(OMC)CC(PSU)ACCUA C(PSU)AUCCAGCG AAACCAC(A2M)G(OMC) CAAGGGAACG GGCU(PSU)GG(OMC)G GAAUCAGCGG (OMG)GAAAGAAGA CCCUGUUGAG C(PSU)UGAC(OMU)CUA GUCUGGCACG GUGAAGAGAC AUGAGAGG U GUAGAAUAAG UGGGAGGCCC CCGGCGCCCC CCCGGUGUCC CCGCGAGGGG CCCGGGGCGG GGUCCGCCGG CCCUGCGGG CCGCCGGUGA AAUACCACUA CUCUGAUCGU UUUUUCACUG ACCCGGUGAG GCGGGGGGGC GAGCCCCGAG GGGCUCUCGC UUCUGGCGC CAAGCGCCCG GCCGCGCGCC GGCCGGGCGC GACCCGCUCC GGGGACAGUG CCAGGUGGGG AGUUUGACUG (OMG)GGCGGUAC ACCUGUCAAA CGGUA(6MZ)CGCA GG(OMU)(OMG)UCCUAA GGCGAGCUCA GGGAGGACAG AAACC UCCC GUGGAGCAGA AGGGCAAAAG CUCGCUUGA(PSU) CU(PSU)GA(PSU)UUUC AG(OMU)ACGAA(PSU)A CAGACC GUG AAAGCGGGGC CUCACGAUCC UUCUGACCUU (PSU)UGGGUUU(PSU)A AGCAGGA(OMG)GU GUCAGAAAAG UUACC ACAG (OMG)GAUAACUGG C(PSU)UGUGGCGG CCAAGCGU(PSU)C A(PSU)AGCGACG(PSU) CGCUUUUUGA (PSU)C CUU(5MC)GAU GUCGG(OMC)(PSU)CUU CCUAUCAUUG (PSU)GAAGCAGAA UUCACCAAGC GU(PSU)(OMG)GAU (OMU)(OMG) (PSU)UCACCCACU AAUAGGGAAC G(PSU)G(A2M)GCUGGG (UR3)U(PSU)AGA(OMC)CGU CGUGAG ACA GGU(PSU)AGUUUU ACCCUACUGA UG(A2M)UGUG(PSU)UG (PSU)UGCCAUGGU AAUCCUGCUC AGUACGAGAG G AACCGCAG (OMG)U(OMU)CA(OMG)ACAU (PSU)UGGUGUAU(OMG) UGCUUGGCUG AGGAGCCAAU GGGGCGAAGC UA CCA(PSU)CUG UGGGAUUAUG AC(PSU)GAACGCC UCUAAGUCAG AAUCCCGCCC AGGCGGAACG AUACGGCAGC GCCGC GGAG CCUCGGUUGG CCUCGGAUAG CCGGUCCCCC GCCUGUCCCC GCCGGCGGGC CGCCCCCCCC CUCCACGCGC CCCGCG CGC GCGGGAGGGC GCGUGCCCCG CCGCGCGCCG GGACCGGGGU CCGGUGCGGA GUGCCCUUCG UCCUGGGAAA CGGGGCG CG GCCGGAGAGG CGGCCGCCCC CUCGCCCGUC ACGCACCGCA CGUUCGUGGG GAACCUGGCG CUAAACCA(PSU)U CGUA GACGA CCUGCUUCUG GGUCGGGG(PSU)U UCGUACGUAG CAGAGCAGCU CCCUCGCUGC GAUCUAUUGA AAGUCAGCCC U CGACACAA GGGUUUGU

Macromolecule #38: 5S rRNA

• Macromolecule: Name: 5S rRNA / type: rna / ID: 38 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 38.691914 KDa

Sequence

String:

GUCUACGGCC AUACCACCCU GAACGCGCCC GAUCUCGUCU GAUCUCGGAA GCUAAGCAGG GUCGGGCCUG GUUAGUACUU GGAUGGGAG ACCGCCUGGG AAUACCGGGU GCUGUAGGCU U

GENBANK: GENBANK: X12811.1

Macromolecule #39: 5.8S rRNA

• Macromolecule: Name: 5.8S rRNA / type: rna / ID: 39 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 50.46384 KDa

Sequence

String:

CGACUCUUAG CGGUGGAUCA CUCGGCUCGU GCGUCGAUGA AGAACGCAGC UAGC(PSU)GCGAG AAUUAAUG(PSU)G AA UU(OMG)CAGG ACACAUUGAU CAUCGACACU UCGAACGCAC UUGCGGCCCC GGGUUCCUCC CGGGGCUACG CCUGUCUGA GCGUCGCUU

GENBANK: GENBANK: U13369.1

Macromolecule #45: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 45 / Number of copies: 186 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #46: 1,4-DIAMINOBUTANE

• Macromolecule: Name: 1,4-DIAMINOBUTANE / type: ligand / ID: 46 / Number of copies: 7 / Formula: PUT
• Molecular weight: Theoretical: 88.151 Da

Chemical component information

ChemComp-PUT:
1,4-DIAMINOBUTANE

Macromolecule #47: POTASSIUM ION

• Macromolecule: Name: POTASSIUM ION / type: ligand / ID: 47 / Number of copies: 46 / Formula: K
• Molecular weight: Theoretical: 39.098 Da

Macromolecule #48: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 48 / Number of copies: 5 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #49: SPERMIDINE

• Macromolecule: Name: SPERMIDINE / type: ligand / ID: 49 / Number of copies: 7 / Formula: SPD
• Molecular weight: Theoretical: 145.246 Da

Chemical component information

ChemComp-SPD:
SPERMIDINE

Macromolecule #50: SPERMINE

• Macromolecule: Name: SPERMINE / type: ligand / ID: 50 / Number of copies: 5 / Formula: SPM
• Molecular weight: Theoretical: 202.34 Da

Chemical component information

ChemComp-SPM:
SPERMINE

Macromolecule #51: ADENOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 51 / Number of copies: 1 / Formula: ATP
• Molecular weight: Theoretical: 507.181 Da

Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

Macromolecule #52: water

• Macromolecule: Name: water / type: ligand / ID: 52 / Number of copies: 11817 / Formula: HOH
• Molecular weight: Theoretical: 18.015 Da

Chemical component information

ChemComp-HOH:
WATER

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 0.4583 mg/mL
• Buffer: pH: 7.5
• Grid: Model: Quantifoil R2/2 / Material: COPPER / Mesh: 200 / Support film - Material: CARBON / Support film - topology: HOLEY / Support film - Film thickness: 2 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 20 sec. / Pretreatment - Atmosphere: AIR / Pretreatment - Pressure: 0.037 kPa; Details: Operated at 15 mA, easiGlow Discharge Cleaning system (PELCO)
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 85 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV
• Details: Hela 60S ribosomal subunits were isolated from cytoplasmic extracts of Hela cells, involving puromycin treatment and sucrose gradient centrifugation in high salt. The gradients were manually fractionated, and separated peak fractions of 60S subunits were collected. For buffer exchange, the sample was diluted 6 x in buffer F (20 mM HEPES pH7.5, 10 mM KCl, 2 mM MgCl2, 2 mM DTT) supplemented with 8.56 % sucrose to adjust the sucrose in the storage buffer to 12%, respectively. Finally, the 60S subunit suspension was concentrated on Amicon Ultracel filter units with a 10 kDa cutoff (Merck Millipore) was snap frozen in 10 ul aliquots and stored at -80 degrees Celsius. Concentrated Hela 60S ribosomal subunits were diluted to 200 nM in buffer E (20 mM HEPES pH7.5, 2 mM MgCl2, 90 mM KCl, 0.5 mM Spermidine, 0.04 mM Spermine, 2 mM DTT) and 4 ul were spotted onto glow-discharged holey carbon grids coated with a continuous thin carbon film (Quantifoil Cu200 R2/2+) and incubated for 40 sec at 4 degrees CelsiusC, 85% RH. The excess of liquid was blotted away for 2 sec and the grids were plunge frozen using the Vitrobot Mark IV (Thermo Fischer Scientific).

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Temperature: Min: 80.0 K / Max: 82.0 K
• Image recording: Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Digitization - Dimensions - Width: 11520 pixel / Digitization - Dimensions - Height: 8184 pixel / Number grids imaged: 1 / Number real images: 16983 / Average electron dose: 49.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: C2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 105000
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 1372803
• Startup model: Type of model: NONE
• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Algorithm: BACK PROJECTION / Resolution.type: BY AUTHOR / Resolution: 1.78 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 3.3) / Number images used: 880876
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 3.3)
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 3.3)
• Final 3D classification: Software - Name: cryoSPARC (ver. 3.1)

Atomic model buiding 1

Initial model

PDB ID:

6eko

Chain - Source name: PDB / Chain - Initial model type: experimental model

• Details: Interactive model building was performed in Coot using an auto sharpened map in cryoSPARC and additionally a low-pass-filtered map to 4 Angstrom for the more mobile regions of the 60S structure. Each residue of the model was checked individually for modifications and adjusted manually with a subsequent refinement in phenix.refine. Polyamines and metals were added while searching for unmodelled blobs in Coot. Water molecules were added using phenix.douse and visually validated in Coot. The model was validated using Molprobity.
• Refinement: Space: REAL / Protocol: OTHER / Target criteria: Cross-correlation coefficient

Output model

PDB-8qyx:
Human 60S ribosomal subunit