Entry Database : EMDB / ID : EMD-18225 Downloads & linksTitle cryo-EM structure of the human spliceosomal B complex protomer (tri-snRNP core region) Map data Details SampleComplex : human pre-catalytic spliceosomeRNA : x 4 typesProtein or peptide : x 17 types Details Keywords spliceosome / pre-catalytic spliceosome / spliceosomal B complex / SPLICINGFunction / homology Function and homology informationFunction Domain/homology Component
microfibril / spliceosomal snRNP complex / ribonucleoprotein complex localization / U4atac snRNP / positive regulation of cytotoxic T cell differentiation / maturation of 5S rRNA / RNA localization / positive regulation of primary miRNA processing / U4atac snRNA binding / box C/D sno(s)RNA binding ... microfibril / spliceosomal snRNP complex / ribonucleoprotein complex localization / U4atac snRNP / positive regulation of cytotoxic T cell differentiation / maturation of 5S rRNA / RNA localization / positive regulation of primary miRNA processing / U4atac snRNA binding / box C/D sno(s)RNA binding / snRNP binding / dense fibrillar component / transcription elongation factor activity / U4/U6 snRNP / positive regulation of androgen receptor activity / U2-type catalytic step 1 spliceosome / RNA splicing, via transesterification reactions / spliceosomal tri-snRNP complex / U4 snRNA binding / box C/D methylation guide snoRNP complex / mRNA cis splicing, via spliceosome / U2-type spliceosomal complex / U2-type precatalytic spliceosome / proline-rich region binding / U2-type prespliceosome assembly / U2-type catalytic step 2 spliceosome / ubiquitin-like protein conjugating enzyme binding / RNA polymerase binding / U4 snRNP / rRNA modification in the nucleus and cytosol / box C/D snoRNP assembly / U2 snRNP / positive regulation of protein targeting to mitochondrion / U3 snoRNA binding / U2-type prespliceosome / K63-linked polyubiquitin modification-dependent protein binding / positive regulation of miRNA metabolic process / precatalytic spliceosome / spliceosomal complex assembly / regulation of alternative mRNA splicing, via spliceosome / mRNA Splicing - Minor Pathway / negative regulation of transcription elongation by RNA polymerase II / mRNA 3'-splice site recognition / MLL1 complex / spliceosomal tri-snRNP complex assembly / single fertilization / Major pathway of rRNA processing in the nucleolus and cytosol / U5 snRNA binding / U5 snRNP / RNA processing / U2 snRNA binding / Cajal body / U6 snRNA binding / spliceosomal snRNP assembly / ribonucleoprotein complex binding / pre-mRNA intronic binding / U1 snRNA binding / U4/U6 x U5 tri-snRNP complex / catalytic step 2 spliceosome / maturation of LSU-rRNA / mRNA Splicing - Major Pathway / RNA splicing / nuclear receptor coactivator activity / maturation of SSU-rRNA / response to cocaine / small-subunit processome / nuclear receptor binding / positive regulation of transcription elongation by RNA polymerase II / transcription coregulator activity / spliceosomal complex / protein modification process / mRNA processing / mRNA splicing, via spliceosome / protein tag activity / ribosomal small subunit biogenesis / transcription corepressor activity / cellular response to xenobiotic stimulus / protein-macromolecule adaptor activity / cellular response to tumor necrosis factor / ATPase binding / cellular response to lipopolysaccharide / RNA polymerase II-specific DNA-binding transcription factor binding / cytosolic large ribosomal subunit / transcription coactivator activity / nuclear speck / cell cycle / cell division / intracellular membrane-bounded organelle / GTPase activity / centrosome / chromatin / nucleolus / GTP binding / Golgi apparatus / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / protein-containing complex / DNA binding / RNA binding / zinc ion binding Similarity search - Function WW domain-binding protein 4 / Ubiquitin-like modifier Hub1/Ubl5 / Pre-mRNA-splicing factor 38, C-terminal / Pre-mRNA-splicing factor 38-associated hydrophilic C-term / Micro-fibrillar-associated protein 1, C-terminal / Microfibrillar-associated protein 1 / Microfibril-associated/Pre-mRNA processing / U4/U6.U5 small nuclear ribonucleoprotein component Snu23 / Pre-mRNA-splicing factor 38 / Transcription elongation regulator 1-like ... WW domain-binding protein 4 / Ubiquitin-like modifier Hub1/Ubl5 / Pre-mRNA-splicing factor 38, C-terminal / Pre-mRNA-splicing factor 38-associated hydrophilic C-term / Micro-fibrillar-associated protein 1, C-terminal / Microfibrillar-associated protein 1 / Microfibril-associated/Pre-mRNA processing / U4/U6.U5 small nuclear ribonucleoprotein component Snu23 / Pre-mRNA-splicing factor 38 / Transcription elongation regulator 1-like / PRP38 family / U1-C, C2H2-type zinc finger / U1 zinc finger / PRP4-like superfamily / pre-mRNA processing factor 4 (PRP4) like / FF domain / SNU66/SART1 family / HIND motif / SART-1 family / HIND motif / FF domain / PWI domain superfamily / FF domain superfamily / PWI domain / PWI domain profile. / FF domain profile. / Contains two conserved F residues / Pre-mRNA processing factor 4 (PRP4)-like / Splicing factor 3A subunit 1, ubiquitin domain / Splicing Factor Motif, present in Prp18 and Pr04 / Pre-mRNA-splicing factor 3 / U4/U6 small nuclear ribonucleoprotein Prp3 / pre-mRNA processing factor 3 domain / Prp31 C-terminal / U4/U6 small nuclear ribonucleoprotein Prp31 / Prp31 C terminal domain / Small nuclear ribonucleoprotein Prp3, C-terminal domain / Small nuclear ribonucleoprotein Prp3, C-terminal domain / PWI domain / PWI, domain in splicing factors / BUD31/G10-related, conserved site / G10 protein signature 1. / G10 protein signature 2. / Dim1 family / Mitosis protein DIM1 / Mitosis protein DIM1 / PRP1 splicing factor, N-terminal / PRP1 splicing factor, N-terminal / Splicing factor 3A subunit 1 / Splicing factor 3A subunit 1, conserved domain / Pre-mRNA splicing factor PRP21 like protein / G10 protein / Pre-mRNA-splicing factor BUD31 / SWAP/Surp / SWAP/Surp superfamily / Surp module / SURP motif repeat profile. / Suppressor-of-White-APricot splicing regulator / Matrin/U1-C, C2H2-type zinc finger / Zinc finger matrin-type profile. / Zinc-finger double-stranded RNA-binding / Zinc finger, double-stranded RNA binding / H/ACA ribonucleoprotein complex, subunit Nhp2-like / Pre-mRNA-splicing factor Syf1-like / NOSIC / NOSIC (NUC001) domain / Nop domain / Nop domain superfamily / Nop, C-terminal domain / snoRNA binding domain, fibrillarin / Nop domain profile. / Snu114, GTP-binding domain / Tetratricopeptide repeat / 116kDa U5 small nuclear ribonucleoprotein component, N-terminal / 116kDa U5 small nuclear ribonucleoprotein component, C-terminal / 116 kDa U5 small nuclear ribonucleoprotein component N-terminus / Matrin/U1-C-like, C2H2-type zinc finger / U1-like zinc finger / HAT (Half-A-TPR) repeat / HAT (Half-A-TPR) repeats / Tetratricopeptide repeat / Elongation Factor G, domain II / Elongation Factor G, domain III / JAB1/Mov34/MPN/PAD-1 ubiquitin protease / Translation elongation factor EFG/EF2, domain IV / Elongation factor G, domain IV / Elongation factor G, domain IV / Elongation factor G C-terminus / WW domain / Elongation factor EFG, domain V-like / Elongation factor G C-terminus / WW/rsp5/WWP domain signature. / EF-G domain III/V-like / WW domain superfamily / WW/rsp5/WWP domain profile. / Domain with 2 conserved Trp (W) residues / WW domain / PROCT domain / Prp8 RNase domain IV, fingers region / PROCT (NUC072) domain Similarity search - Domain/homology Transcription elongation regulator 1 / U4/U6 small nuclear ribonucleoprotein Prp4 / U4/U6.U5 tri-snRNP-associated protein 1 / U4/U6 small nuclear ribonucleoprotein Prp3 / WW domain-binding protein 4 / Pre-mRNA-processing factor 6 / Protein BUD31 homolog / Microfibrillar-associated protein 1 / NHP2-like protein 1 / Thioredoxin-like protein 4A ... Transcription elongation regulator 1 / U4/U6 small nuclear ribonucleoprotein Prp4 / U4/U6.U5 tri-snRNP-associated protein 1 / U4/U6 small nuclear ribonucleoprotein Prp3 / WW domain-binding protein 4 / Pre-mRNA-processing factor 6 / Protein BUD31 homolog / Microfibrillar-associated protein 1 / NHP2-like protein 1 / Thioredoxin-like protein 4A / 116 kDa U5 small nuclear ribonucleoprotein component / Splicing factor 3A subunit 1 / Pre-mRNA-processing-splicing factor 8 / Pre-mRNA-splicing factor 38A / U4/U6 small nuclear ribonucleoprotein Prp31 / Zinc finger matrin-type protein 2 / Ubiquitin-like protein 5 Similarity search - ComponentBiological species Homo sapiens (human)Method single particle reconstruction / cryo EM / Resolution : 3.1 Å Details AuthorsZhang Z / Kumar V / Dybkov O / Will CL / Urlaub H / Stark H / Luehrmann R Funding support Germany, 1 items Details Hide detailsOrganization Grant number Country German Research Foundation (DFG) Germany
CitationJournal : To Be Published Title : New insights into the functions of B complex proteins revealed by cryo-EM of dimerized spliceosomesAuthors : Zhang Z / Kumar V / Dybkov O / Will CL / Urlaub H / Stark H / Luehrmann R History Deposition Aug 16, 2023 - Header (metadata) release Jan 24, 2024 - Map release Jan 24, 2024 - Update Jan 24, 2024 - Current status Jan 24, 2024 Processing site : PDBe / Status : Released
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