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- EMDB-17730: masked refinement giving rise to better defined protruding densit... -
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Open data
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Basic information
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Title | masked refinement giving rise to better defined protruding densities of the potential macrodomain outside the AUD helical assemblies. | |||||||||
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![]() | Helical scaffold / Replication complex / Alpha granules / Viral factories / VIRAL PROTEIN | |||||||||
Biological species | ![]() | |||||||||
Method | helical reconstruction / cryo EM / Resolution: 2.98 Å | |||||||||
![]() | Reguera J / Hons M / Zimberger C / Ptchelkine D / Jones R / Desfosses A | |||||||||
Funding support | ![]()
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![]() | ![]() Title: The alphavirus nsP3 protein forms helical tubular scaffolds important for viral replication and particle assembly Authors: Reguera J / Hons M / Zimberger C / Ptchelkine D / Jones R / Desfosses A | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 203.3 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 18.8 KB 18.8 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 14.4 KB | Display | ![]() |
Images | ![]() | 119.9 KB | ||
Filedesc metadata | ![]() | 5.3 KB | ||
Others | ![]() ![]() ![]() ![]() | 5.7 MB 200.2 MB 204.5 MB 204.5 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 766.1 KB | Display | ![]() |
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Full document | ![]() | 765.6 KB | Display | |
Data in XML | ![]() | 21.7 KB | Display | |
Data in CIF | ![]() | 28.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Voxel size | X=Y=Z: 1.06 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: Class of a mask including 16 protomers Map alligned to EMD-17729
File | emd_17730_additional_1.map | ||||||||||||
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Annotation | Class of a mask including 16 protomers Map alligned to EMD-17729 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Additional map: Map alligned to EMD-17729
File | emd_17730_additional_2.map | ||||||||||||
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Annotation | Map alligned to EMD-17729 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_17730_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_17730_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Helical scaffold assembly of CHIKV nsP3 mediated by its Unique al...
Entire | Name: Helical scaffold assembly of CHIKV nsP3 mediated by its Unique alphavirus domain. |
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Components |
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-Supramolecule #1: Helical scaffold assembly of CHIKV nsP3 mediated by its Unique al...
Supramolecule | Name: Helical scaffold assembly of CHIKV nsP3 mediated by its Unique alphavirus domain. type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 220.25 kDa/nm |
-Macromolecule #1: Chikungunya virus non structural protein 3
Macromolecule | Name: Chikungunya virus non structural protein 3 / type: protein_or_peptide / ID: 1 / Details: Zn / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Recombinant expression | Organism: ![]() |
Sequence | String: APSYRVKRMD IAKNDEECVV NAANPRGLPG DGVCKAVYKK WPESFKNSAT PVGTAKTVMC GTYPVIHAVG PNFSNYSESE GDRELAAAYR EVAKEVTRLG VNSVAIPLLS TGVYSGGKDR LTQSLNHLFT AMDSTDADVV IYCRDKEWEK KISEAIQMRT QVELLDEHIS ...String: APSYRVKRMD IAKNDEECVV NAANPRGLPG DGVCKAVYKK WPESFKNSAT PVGTAKTVMC GTYPVIHAVG PNFSNYSESE GDRELAAAYR EVAKEVTRLG VNSVAIPLLS TGVYSGGKDR LTQSLNHLFT AMDSTDADVV IYCRDKEWEK KISEAIQMRT QVELLDEHIS IDCDVVRVHP DSSLAGRKGY STTEGALYSY LEGTRFHQTA VDMAEIYTMW PKQTEANEQV CLYALGESIE SIRQKCPVDD ADASSPPKTV PCLCRYAMTP ERVTRLRMNH VTSIIVCSSF PLPKYKIEGV QKVKCSKVML FDHNVPSRVS PREYRPSQES VQEASTTTSL THSQFDLSVD GKILPVPSDL DADAPALEPA LDDGAIHTLP SATGNLAAVS DWVMSTVPVA PPRRRRGRNL TVTCDEREGN ITPMASVRFF RAELCPVVQE TAETRDTAMS LQAPPSTATE LSHPPISFGA PSETFPITFG DFNEGEIESL SSELLTFGDF LPGEVDDLTD SDWSTCSDTD DEL |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | helical reconstruction |
Aggregation state | helical array |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE-PROPANE |
Details | his sample was heterogeneous in lenght |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 18.1 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.8 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |