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- EMDB-17725: CryoEM reconstruction of Influenza A virus (HK68) hemagglutinin b... -

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Basic information

Entry
Database: EMDB / ID: EMD-17725
TitleCryoEM reconstruction of Influenza A virus (HK68) hemagglutinin bound to an Affimer reagent
Map data
Sample
  • Complex: CryoEM reconstruction of Influenza A virus (HK68) hemagglutinin bound to an Affimer reagent
KeywordsInhibitor / Antiviral / Influenza A Virus / Complex / ANTIVIRAL PROTEIN
Biological speciesInfluenza A virus
Methodsingle particle reconstruction / cryo EM / Resolution: 4.4 Å
AuthorsDebski-Antoniak O / Flynn A / Klebl PD / Tiede C / Muench S / Tomlinson D / Fontana J
Funding support1 items
OrganizationGrant numberCountry
Other private
CitationJournal: To Be Published
Title: Exploiting the Affimer platform against influenza A virus
Authors: Debski-Antoniak O / Flynn A / Klebl PD / Tiede C / Muench S / Tomlinson D / Fontana J
History
DepositionJun 24, 2023-
Header (metadata) releaseJul 10, 2024-
Map releaseJul 10, 2024-
UpdateJul 10, 2024-
Current statusJul 10, 2024Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_17725.png

Downloads & links

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Map

FileDownload / File: emd_17725.map.gz / Format: CCP4 / Size: 30.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 0.83 Å
Density
Contour LevelBy AUTHOR: 0.0106
Minimum - Maximum-0.024936475 - 1.7399822
Average (Standard dev.)0.0015045465 (±0.024789704)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions200200200
Spacing200200200
CellA=B=C: 166.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : CryoEM reconstruction of Influenza A virus (HK68) hemagglutinin b...

EntireName: CryoEM reconstruction of Influenza A virus (HK68) hemagglutinin bound to an Affimer reagent
Components
  • Complex: CryoEM reconstruction of Influenza A virus (HK68) hemagglutinin bound to an Affimer reagent

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Supramolecule #1: CryoEM reconstruction of Influenza A virus (HK68) hemagglutinin b...

SupramoleculeName: CryoEM reconstruction of Influenza A virus (HK68) hemagglutinin bound to an Affimer reagent
type: complex / ID: 1 / Parent: 0
Source (natural)Organism: Influenza A virus

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 56.2 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: OTHER / Nominal defocus max: 4.0 µm / Nominal defocus min: 2.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.4 Å / Resolution method: FSC 0.5 CUT-OFF / Number images used: 206111
Initial angle assignmentType: ANGULAR RECONSTITUTION
Final angle assignmentType: ANGULAR RECONSTITUTION
FSC plot (resolution estimation)

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