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データを開く
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基本情報
登録情報 | ![]() | |||||||||
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タイトル | cA4-bound Cami1 in complex with 70S ribosome | |||||||||
![]() | Sharpened composite map | |||||||||
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![]() | CRISPR / cyclic oligoadenylate / RNAse / RelE-toxin / HYDROLASE / RIBOSOME | |||||||||
機能・相同性 | ![]() negative regulation of cytoplasmic translational initiation / stringent response / ornithine decarboxylase inhibitor activity / transcription antitermination factor activity, RNA binding / misfolded RNA binding / Group I intron splicing / RNA folding / transcriptional attenuation / endoribonuclease inhibitor activity / RNA-binding transcription regulator activity ...negative regulation of cytoplasmic translational initiation / stringent response / ornithine decarboxylase inhibitor activity / transcription antitermination factor activity, RNA binding / misfolded RNA binding / Group I intron splicing / RNA folding / transcriptional attenuation / endoribonuclease inhibitor activity / RNA-binding transcription regulator activity / positive regulation of ribosome biogenesis / negative regulation of cytoplasmic translation / four-way junction DNA binding / translational termination / DnaA-L2 complex / translation repressor activity / negative regulation of translational initiation / negative regulation of DNA-templated DNA replication initiation / regulation of mRNA stability / mRNA regulatory element binding translation repressor activity / ribosome assembly / positive regulation of RNA splicing / assembly of large subunit precursor of preribosome / transcription elongation factor complex / cytosolic ribosome assembly / regulation of DNA-templated transcription elongation / DNA endonuclease activity / ribosomal large subunit assembly / transcription antitermination / response to reactive oxygen species / translational initiation / regulation of cell growth / DNA-templated transcription termination / maintenance of translational fidelity / response to radiation / mRNA 5'-UTR binding / large ribosomal subunit / ribosome biogenesis / ribosome binding / regulation of translation / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / transferase activity / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / molecular adaptor activity / rRNA binding / negative regulation of translation / ribosome / structural constituent of ribosome / translation / response to antibiotic / negative regulation of DNA-templated transcription / mRNA binding / protein homodimerization activity / DNA binding / RNA binding / zinc ion binding / membrane / cytosol / cytoplasm 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.67 Å | |||||||||
![]() | Tamulaitiene G / Mogila I / Sasnauskas G / Tamulaitis G | |||||||||
資金援助 | リトアニア, 1件
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![]() | ![]() タイトル: Ribosomal stalk-captured CARF-RelE ribonuclease inhibits translation following CRISPR signaling. 著者: Irmantas Mogila / Giedre Tamulaitiene / Konstanty Keda / Albertas Timinskas / Audrone Ruksenaite / Giedrius Sasnauskas / Česlovas Venclovas / Virginijus Siksnys / Gintautas Tamulaitis 要旨: Prokaryotic type III CRISPR-Cas antiviral systems employ cyclic oligoadenylate (cA) signaling to activate a diverse range of auxiliary proteins that reinforce the CRISPR-Cas defense. Here we ...Prokaryotic type III CRISPR-Cas antiviral systems employ cyclic oligoadenylate (cA) signaling to activate a diverse range of auxiliary proteins that reinforce the CRISPR-Cas defense. Here we characterize a class of cA-dependent effector proteins named CRISPR-Cas-associated messenger RNA (mRNA) interferase 1 (Cami1) consisting of a CRISPR-associated Rossmann fold sensor domain fused to winged helix-turn-helix and a RelE-family mRNA interferase domain. Upon activation by cyclic tetra-adenylate (cA), Cami1 cleaves mRNA exposed at the ribosomal A-site thereby depleting mRNA and leading to cell growth arrest. The structures of apo-Cami1 and the ribosome-bound Cami1-cA complex delineate the conformational changes that lead to Cami1 activation and the mechanism of Cami1 binding to a bacterial ribosome, revealing unexpected parallels with eukaryotic ribosome-inactivating proteins. | |||||||||
履歴 |
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構造の表示
添付画像 |
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ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 228.3 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 82.3 KB 82.3 KB | 表示 表示 | ![]() |
画像 | ![]() | 84.6 KB | ||
Filedesc metadata | ![]() | 15.4 KB | ||
その他 | ![]() | 116.2 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 8phjMC ![]() 8phbC C: 同じ文献を引用 ( M: このマップから作成された原子モデル |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||
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注釈 | Sharpened composite map | ||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.12 Å | ||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-追加マップ: Unsharpened composite map
ファイル | emd_17667_additional_1.map | ||||||||||||
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注釈 | Unsharpened composite map | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
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試料の構成要素
+全体 : Cami1 bound in 70S E.coli ribosome
+超分子 #1: Cami1 bound in 70S E.coli ribosome
+超分子 #2: 70S E.coli ribosome
+超分子 #3: synthetic mRNA
+超分子 #4: cA4
+超分子 #5: CRISPR-associated protein Cami1, APE2256 family
+分子 #1: Large ribosomal subunit protein bL33
+分子 #2: Large ribosomal subunit protein bL34
+分子 #3: Large ribosomal subunit protein bL35
+分子 #4: Large ribosomal subunit protein bL36A
+分子 #5: Large ribosomal subunit protein bL31A
+分子 #6: Large ribosomal subunit protein uL10
+分子 #7: Large ribosomal subunit protein uL11
+分子 #11: Small ribosomal subunit protein uS2
+分子 #12: Small ribosomal subunit protein uS3
+分子 #13: Small ribosomal subunit protein uS4
+分子 #14: Small ribosomal subunit protein uS5
+分子 #15: Small ribosomal subunit protein bS6, fully modified isoform
+分子 #16: Small ribosomal subunit protein uS7
+分子 #17: Small ribosomal subunit protein uS8
+分子 #18: Small ribosomal subunit protein uS9
+分子 #19: Small ribosomal subunit protein uS10
+分子 #20: Small ribosomal subunit protein uS11
+分子 #21: Small ribosomal subunit protein uS12
+分子 #22: Small ribosomal subunit protein uS13
+分子 #23: Small ribosomal subunit protein uS14
+分子 #24: Small ribosomal subunit protein uS15
+分子 #25: Small ribosomal subunit protein bS16
+分子 #26: Small ribosomal subunit protein uS17
+分子 #27: Small ribosomal subunit protein bS18
+分子 #28: Small ribosomal subunit protein uS19
+分子 #29: Small ribosomal subunit protein bS20
+分子 #30: Small ribosomal subunit protein bS21
+分子 #31: Large ribosomal subunit protein bL12
+分子 #32: CRISPR-associated protein, APE2256 family
+分子 #35: Large ribosomal subunit protein uL2
+分子 #36: Large ribosomal subunit protein uL3
+分子 #37: Large ribosomal subunit protein uL4
+分子 #38: Large ribosomal subunit protein uL5
+分子 #39: Large ribosomal subunit protein uL6
+分子 #40: Large ribosomal subunit protein bL9
+分子 #41: Large ribosomal subunit protein uL13
+分子 #42: Large ribosomal subunit protein uL14
+分子 #43: Large ribosomal subunit protein uL15
+分子 #44: Large ribosomal subunit protein uL16
+分子 #45: Large ribosomal subunit protein bL17
+分子 #46: Large ribosomal subunit protein uL18
+分子 #47: Large ribosomal subunit protein bL19
+分子 #48: Large ribosomal subunit protein bL20
+分子 #49: Large ribosomal subunit protein bL21
+分子 #50: Large ribosomal subunit protein uL22
+分子 #51: Large ribosomal subunit protein uL23
+分子 #52: Large ribosomal subunit protein uL24
+分子 #53: Large ribosomal subunit protein bL25
+分子 #54: Large ribosomal subunit protein bL27
+分子 #55: Large ribosomal subunit protein bL28
+分子 #56: Large ribosomal subunit protein uL29
+分子 #57: Large ribosomal subunit protein uL30
+分子 #58: Large ribosomal subunit protein bL32
+分子 #8: fMet-tRNA(fMet)
+分子 #9: mRNA
+分子 #10: 16S rRNA
+分子 #33: 23S rRNA (2862-MER)
+分子 #34: 5S rRNA
+分子 #59: Cyclic tetraadenosine monophosphate (cA4)
+分子 #60: ZINC ION
+分子 #61: MAGNESIUM ION
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.5 |
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グリッド | 材質: COPPER / メッシュ: 300 / 支持フィルム - 材質: CARBON / 支持フィルム - トポロジー: HOLEY / 詳細: GLOW DISCHARGED 60 seconds |
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 95 % / チャンバー内温度: 277 K / 装置: FEI VITROBOT MARK IV |
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電子顕微鏡法
顕微鏡 | TFS GLACIOS |
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撮影 | フィルム・検出器のモデル: FEI FALCON III (4k x 4k) 検出モード: COUNTING / 平均電子線量: 30.0 e/Å2 |
電子線 | 加速電圧: 200 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2.0 µm / 最小 デフォーカス(公称値): 1.0 µm |
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画像解析
初期モデル | モデルのタイプ: PDB ENTRY PDBモデル - PDB ID: |
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最終 再構成 | 解像度のタイプ: BY AUTHOR / 解像度: 3.67 Å / 解像度の算出法: OTHER 詳細: Composite map, resolution determined by the local refimenet map of lowest resolution 使用した粒子像数: 158387 |
初期 角度割当 | タイプ: MAXIMUM LIKELIHOOD |
最終 角度割当 | タイプ: MAXIMUM LIKELIHOOD |