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Open data
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Basic information
Entry | ![]() | ||||||||||||||||||||||||
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Title | DNA-origami turbine with left-handed chirality | ||||||||||||||||||||||||
![]() | DNA-origami turbine with left-handed chirality | ||||||||||||||||||||||||
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![]() | DNA-origami / DNA nanoturbine / nanorobotics / nanomachine / rotary motor / hydrodynamic flows / DNA | ||||||||||||||||||||||||
Biological species | ![]() | ||||||||||||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 15.0 Å | ||||||||||||||||||||||||
![]() | Shi X / Pumm A-K / Maffeo C / Kohler F / Feigl E / Zhao W / Verschueren D / Golestanian R / Aksimentiev A / Dietz H / Dekker C | ||||||||||||||||||||||||
Funding support | European Union, ![]() ![]() ![]()
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![]() | ![]() Title: A nanopore-powered DNA turbine Authors: Shi X / Pumm A-K / Maffeo C / Kohler F / Feigl E / Zhao W / Verschueren D / Golestanian R / Aksimentiev A / Dietz H / Dekker C | ||||||||||||||||||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 15.9 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 15.8 KB 15.8 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 14.3 KB | Display | ![]() |
Images | ![]() | 61.1 KB | ||
Others | ![]() ![]() | 193.6 MB 193.6 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 568.5 KB | Display | ![]() |
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Full document | ![]() | 568.1 KB | Display | |
Data in XML | ![]() | 21.8 KB | Display | |
Data in CIF | ![]() | 28.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | DNA-origami turbine with left-handed chirality | ||||||||||||||||||||
Voxel size | X=Y=Z: 2.28 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: DNA-origami turbine with left-handed chirality - unfiltered half...
File | emd_17600_half_map_1.map | ||||||||||||
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Annotation | DNA-origami turbine with left-handed chirality - unfiltered half map 2 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: DNA-origami turbine with left-handed chirality - unfiltered half...
File | emd_17600_half_map_2.map | ||||||||||||
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Annotation | DNA-origami turbine with left-handed chirality - unfiltered half map 1 | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : DNA Origami
Entire | Name: DNA Origami |
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Components |
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-Supramolecule #1: DNA Origami
Supramolecule | Name: DNA Origami / type: complex / ID: 1 / Parent: 0 |
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-Supramolecule #2: scaffold DNA single strand
Supramolecule | Name: scaffold DNA single strand / type: complex / ID: 2 / Parent: 1 / Details: m13mp18-derived, 8064 bases |
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Source (natural) | Organism: ![]() |
-Supramolecule #3: staple oligonucleotides
Supramolecule | Name: staple oligonucleotides / type: complex / ID: 3 / Parent: 1 / Details: chemically synthesized |
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Source (natural) | Organism: synthetic construct (others) |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 8 |
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Grid | Model: C-flat-1.2/1.3 / Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Support film - topology: HOLEY / Support film - Film thickness: 40 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Atmosphere: AIR |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TITAN |
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Image recording | Film or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Average electron dose: 60.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.5 µm |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |