- EMDB-1760: Double filaments of C-terminally GFP-fused TubZ -
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Basic information
Entry
Database: EMDB / ID: EMD-1760
Title
Double filaments of C-terminally GFP-fused TubZ
Map data
Double helical filaments of a C-Terminal fusion of GFPmut2 to Bacillus thuringiensis serovar israelensis (ATCC 35646) TubZ (Q8KNP3_BACTI) Density for the GFP fusion of TubZ to remove positive density on the outer boundary of the negative stain and unconnected to the main helix.
Journal: Proc Natl Acad Sci U S A / Year: 2010 Title: Filament structure of bacterial tubulin homologue TubZ. Authors: Christopher H S Aylett / Qing Wang / Katharine A Michie / Linda A Amos / Jan Löwe / Abstract: Low copy number plasmids often depend on accurate partitioning systems for their continued survival. Generally, such systems consist of a centromere-like region of DNA, a DNA-binding adaptor, and a ...Low copy number plasmids often depend on accurate partitioning systems for their continued survival. Generally, such systems consist of a centromere-like region of DNA, a DNA-binding adaptor, and a polymerizing cytomotive filament. Together these components drive newly replicated plasmids to opposite ends of the dividing cell. The Bacillus thuringiensis plasmid pBToxis relies on a filament of the tubulin/FtsZ-like protein TubZ for its segregation. By combining crystallography and electron microscopy, we have determined the structure of this filament. We explain how GTP hydrolysis weakens the subunit-subunit contact and also shed light on the partitioning of the plasmid-adaptor complex. The double helical superstructure of TubZ filaments is unusual for tubulin-like proteins. Filaments of ParM, the actin-like partitioning protein, are also double helical. We suggest that convergent evolution shapes these different types of cytomotive filaments toward a general mechanism for plasmid separation.
History
Deposition
Jul 9, 2010
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Header (metadata) release
Jul 23, 2010
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Map release
Oct 29, 2010
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Update
Apr 20, 2016
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Current status
Apr 20, 2016
Processing site: PDBe / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
Download / File: emd_1760.map.gz / Format: CCP4 / Size: 666 KB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation
Double helical filaments of a C-Terminal fusion of GFPmut2 to Bacillus thuringiensis serovar israelensis (ATCC 35646) TubZ (Q8KNP3_BACTI) Density for the GFP fusion of TubZ to remove positive density on the outer boundary of the negative stain and unconnected to the main helix.
Specimen holder: Eucentric / Specimen holder model: SIDE ENTRY, EUCENTRIC
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Image processing
Final reconstruction
Applied symmetry - Helical parameters - Axial symmetry: C2 (2 fold cyclic) Algorithm: OTHER / Resolution.type: BY AUTHOR / Resolution: 35.0 Å / Resolution method: OTHER / Software - Name: MRC Details: Final map was calculated from two averaged filaments
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