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- EMDB-17363: Staphylococcus aureus 70S ribosome with elongation factor G locke... -

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Open data

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Basic information

Entry

Database: EMDB / ID: EMD-17363

Title

Staphylococcus aureus 70S ribosome with elongation factor G locked with fusidic acid cyclopentane in post-translocational state

Map data

Local filtered map

Sample

Complex: Staphylococcus aureus 70S ribosome with elongation factor G and fusidic acid cyclopentane
- Protein or peptide: x 48 types
- RNA: x 5 types
Ligand: x 8 types

Keywords

RIBOSOME /

fusidic acid /

EF-G /

antibiotic

Function / homology

Function and homology information

ribosome disassembly /

translation elongation factor activity /

ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding /

ribosomal small subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / large ribosomal subunit / small ribosomal subunit / cytoplasmic translation ...
Similarity search - Function

Ribosomal protein L31 type B /

: / Translation elongation factor EFG/EF2 /

Elongation factor G, domain III / EFG, domain V /

Elongation Factor G, domain II /

Elongation Factor G, domain III / Translation elongation factor EFG/EF2, domain IV /

Elongation factor G, domain IV /

Elongation factor G, domain IV ...

Ribosomal protein L31 type B /

: / Translation elongation factor EFG/EF2 /

Elongation factor G, domain III / EFG, domain V /

Elongation Factor G, domain II /

Elongation Factor G, domain III / Translation elongation factor EFG/EF2, domain IV /

Elongation factor G, domain IV /

Elongation factor G, domain IV / Elongation factor G C-terminus /

Ribosomal protein L25, long-form / Elongation factor EFG, domain V-like / Elongation factor G C-terminus /

Ribosomal protein L25, beta domain /

Ribosomal protein L25, C-terminal /

Ribosomal protein TL5, C-terminal domain / EF-G domain III/V-like / Tr-type G domain, conserved site / Translational (tr)-type guanine nucleotide-binding (G) domain signature. /

Ribosomal protein S14, type Z / Translation elongation factor EFTu-like, domain 2 / Elongation factor Tu domain 2 / Translational (tr)-type GTP-binding domain / Elongation factor Tu GTP binding domain / Translational (tr)-type guanine nucleotide-binding (G) domain profile. /

Ribosomal protein L31 signature. /

Ribosomal protein L31 /

Ribosomal protein L31 superfamily /

Ribosomal protein L31 /

Ribosomal protein L16 signature 1. /

: /

Ribosomal protein L6, conserved site /

Ribosomal protein L6 signature 1. /

Ribosomal protein L16, conserved site /

Ribosomal protein L16 signature 2. /

Ribosomal protein L17 signature. / Ribosomal L25p family /

Ribosomal protein L25 /

Ribosomal protein S14/S29 /

Ribosomal protein L28/L24 superfamily /

Ribosomal protein L36 signature. /

Ribosomal protein L25/Gln-tRNA synthetase, N-terminal /

Ribosomal protein L32p, bacterial type /

Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily /

Ribosomal protein L28 /

Ribosomal protein L35, conserved site /

Ribosomal protein L35 signature. /

Ribosomal protein L33, conserved site /

Ribosomal protein L33 signature. /

Ribosomal protein L35, non-mitochondrial /

Ribosomal protein L5, bacterial-type /

Ribosomal protein L6, bacterial-type /

Ribosomal protein L18, bacterial-type /

Ribosomal protein L19, conserved site /

Ribosomal protein L19 signature. /

Ribosomal protein L36 /

Ribosomal protein L36 superfamily /

Ribosomal protein L36 /

Ribosomal protein L20 signature. /

Ribosomal protein S3, bacterial-type /

Ribosomal protein S6, conserved site /

Ribosomal protein S6 signature. /

Ribosomal protein L27, conserved site /

Ribosomal protein L27 signature. /

Ribosomal protein S19, bacterial-type /

Ribosomal protein S7, bacterial/organellar-type /

Ribosomal protein S11, bacterial-type /

Ribosomal protein S13, bacterial-type /

Ribosomal protein S20 /

Ribosomal protein S20 superfamily /

Ribosomal protein S20 /

Ribosomal protein S9, bacterial/plastid /

Ribosomal protein L14P, bacterial-type /

Ribosomal protein S4, bacterial-type /

Ribosomal protein L34, conserved site /

Ribosomal protein L34 signature. /

30S ribosomal protein S17 /

Ribosomal protein S5, bacterial-type /

Ribosomal protein L22, bacterial/chloroplast-type /

Ribosomal protein S6, plastid/chloroplast /

Ribosomal protein L35 /

Ribosomal protein L35 superfamily /

Ribosomal protein L2, bacterial/organellar-type /

Ribosomal protein L35 /

Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal L28 family /

Ribosomal protein L33 /

Ribosomal protein L28/L24 /

Ribosomal protein L33 superfamily /

: /

Ribosomal protein L30, bacterial-type /

Ribosomal protein L16 /

Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast /

Ribosomal protein S18, conserved site /

Ribosomal protein S18 signature. / L28p-like /

Ribosomal protein L20
Similarity search - Domain/homology

Small ribosomal subunit protein uS10 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL30 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 ...
Similarity search - Component

Biological species

Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) /

Escherichia coli (E. coli)

Method

single particle reconstruction /

cryo EM / Resolution: 2.44 Å

Authors

Gonzalez-Lopez A / Selmer M

Funding support

Sweden, 4 items

Citation

Journal: To Be Published
Title: Cryo-EM structures of Staphylococcus aureus 70S ribosomes in complex with elongation factor G and fusidic acid
Authors: Gonzalez-Lopez A / Larsson D / Nelson B / Hergenrother PJ / Selmer M

History

Deposition	May 16, 2023	-
Header (metadata) release	May 1, 2024	-
Map release	May 1, 2024	-
Update	May 1, 2024	-
Current status	May 1, 2024	Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images	emd_17363.png

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Downloads & links

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EMDB archive

Map data	emd_17363.map.gz	62.2 MB		EMDB map data format
Header (meta data)	emd-17363-v30.xml emd-17363.xml	85.6 KB 85.6 KB	Display Display	EMDB header
FSC (resolution estimation)	emd_17363_fsc.xml	26.1 KB	Display	FSC data file
Images	emd_17363.png	168.6 KB
Masks	emd_17363_msk_1.map	1.3 GB		Mask map
Filedesc metadata	emd-17363.cif.gz	16 KB
Others	emd_17363_additional_1.map.gz emd_17363_additional_2.map.gz emd_17363_half_map_1.map.gz emd_17363_half_map_2.map.gz	654.6 MB 656.8 MB 1.2 GB 1.2 GB
Archive directory	http://ftp.pdbj.org/pub/emdb/structures/EMD-17363 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-17363	HTTPS FTP

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Related structure data

Related structure data	8p2fMC 17364C 8p2gC M: atomic model generated by this map C: citing same article (ref.)
Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

EMDB pages	EMDB (EBI/PDBe) / EMDataResource
Related items in Molecule of the Month	#81 - Sep 2006 Elongation Factors similarity (19) #121 - Jan 2010 70S Ribosomes similarity (30) #253 - Jan 2021 Expressome similarity (12) #157 - Jan 2013 Transfer-Messenger RNA similarity (23) #245 - May 2020 Spliceosomes similarity (12) #104 - Aug 2008 Selenocysteine Synthase similarity (5) #262 - Oct 2021 Fifty Years of Open Access to PDB Structures similarity (10) #146 - Feb 2012 Aminoglycoside Antibiotics similarity (1) #226 - Oct 2018 Aminoglycoside Antibiotics and Resistance similarity (1) #10 - Oct 2000 Ribosome similarity (1)

EMDB pages

EMDB (EBI/PDBe) /

EMDataResource

Related items in Molecule of the Month

#81 - Sep 2006
Elongation Factors
similarity (19)
#121 - Jan 2010
70S Ribosomes
similarity (30)
#253 - Jan 2021
Expressome
similarity (12)
#157 - Jan 2013
Transfer-Messenger RNA
similarity (23)
#245 - May 2020
Spliceosomes
similarity (12)
#104 - Aug 2008
Selenocysteine Synthase
similarity (5)
#262 - Oct 2021
Fifty Years of Open Access to PDB Structures
similarity (10)
#146 - Feb 2012
Aminoglycoside Antibiotics
similarity (1)
#226 - Oct 2018
Aminoglycoside Antibiotics and Resistance
similarity (1)
#10 - Oct 2000
Ribosome
similarity (1)

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Map

File

Download / File: emd_17363.map.gz / Format: CCP4 / Size: 1.3 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)

Annotation

Local filtered map

Projections & slices

Image control

Size
Brightness
Contrast
Others	InvertY flip

Axes	Z (Sec.)	Y (Row.)	X (Col.)
	0.73 Å/pix. x 700 pix. = 509.6 Å	0.73 Å/pix. x 700 pix. = 509.6 Å	0.73 Å/pix. x 700 pix. = 509.6 Å
Surface
Projections
Slices (1/3)
Slices (1/2)
Slices (2/3)

Images are generated by Spider.

Voxel size

X=Y=Z: 0.728 Å

Density

Histogram

Histogram (log scale)

Contour Level	By AUTHOR: 0.2
Minimum - Maximum	-1.5997443 - 3.2361841
Average (Standard dev.)	0.0030918824 (±0.049866907)

Symmetry

Space group: 1

Details

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Supplemental data

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Mask #1

File

emd_17363_msk_1.map

Projections & Slices

Axes	Z	Y	X
Projections
Slices (1/2)

Density Histograms

Histogram

Histogram (log scale)

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Additional map: Local map around EF-G

File

emd_17363_additional_1.map

Annotation

Local map around EF-G

Projections & Slices

Axes	Z	Y	X
Projections
Slices (1/2)

Density Histograms

Histogram

Histogram (log scale)

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Additional map: Unsharpened map

File

emd_17363_additional_2.map

Annotation

Unsharpened map

Projections & Slices

Axes	Z	Y	X
Projections
Slices (1/2)

Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map A

File

emd_17363_half_map_1.map

Annotation

Half map A

Projections & Slices

Axes	Z	Y	X
Projections
Slices (1/2)

Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map B

File

emd_17363_half_map_2.map

Annotation

Half map B

Projections & Slices

Axes	Z	Y	X
Projections
Slices (1/2)

Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Staphylococcus aureus 70S ribosome with elongation factor G and f...

Entire	Name: Staphylococcus aureus 70S ribosome with elongation factor G and fusidic acid cyclopentane
Components	Complex: Staphylococcus aureus 70S ribosome with elongation factor G and fusidic acid cyclopentane Protein or peptide: 50S ribosomal protein L28 Protein or peptide: 50S ribosomal protein L29 Protein or peptide: 50S ribosomal protein L30 Protein or peptide: 50S ribosomal protein L31 type BRibosome Protein or peptide: 50S ribosomal protein L32 Protein or peptide: 50S ribosomal protein L33 1Ribosome Protein or peptide: 50S ribosomal protein L34 Protein or peptide: 50S ribosomal protein L35 Protein or peptide: 50S ribosomal protein L36 RNA: 23S ribosomal RNA RNA: 5S ribosomal RNA RNA: tRNATransfer RNA Protein or peptide: Elongation factor GEF-G Protein or peptide: 50S ribosomal protein L2 Protein or peptide: 50S ribosomal protein L3 Protein or peptide: 50S ribosomal protein L4 Protein or peptide: 50S ribosomal protein L5 Protein or peptide: 50S ribosomal protein L6 Protein or peptide: 50S ribosomal protein L13 Protein or peptide: 50S ribosomal protein L14 Protein or peptide: 50S ribosomal protein L15 Protein or peptide: 50S ribosomal protein L16 Protein or peptide: 50S ribosomal protein L17 Protein or peptide: 50S ribosomal protein L18 Protein or peptide: 50S ribosomal protein L19 Protein or peptide: 50S ribosomal protein L20 Protein or peptide: 50S ribosomal protein L21 Protein or peptide: 50S ribosomal protein L22 Protein or peptide: 50S ribosomal protein L23 Protein or peptide: 50S ribosomal protein L24 Protein or peptide: 50S ribosomal protein L25 Protein or peptide: 50S ribosomal protein L27 RNA: 16S ribosomal RNA RNA: mRNAMessenger RNA Protein or peptide: 30S ribosomal protein S2 Protein or peptide: 30S ribosomal protein S3 Protein or peptide: 30S ribosomal protein S4 Protein or peptide: 30S ribosomal protein S5 Protein or peptide: 30S ribosomal protein S6 Protein or peptide: 30S ribosomal protein S7 Protein or peptide: 30S ribosomal protein S8 Protein or peptide: 30S ribosomal protein S9 Protein or peptide: 30S ribosomal protein S10 Protein or peptide: 30S ribosomal protein S11 Protein or peptide: 30S ribosomal protein S12 Protein or peptide: 30S ribosomal protein S13 Protein or peptide: 30S ribosomal protein S14 type ZRibosome Protein or peptide: 30S ribosomal protein S15 Protein or peptide: 30S ribosomal protein S16 Protein or peptide: 30S ribosomal protein S17 Protein or peptide: 30S ribosomal protein S18 Protein or peptide: 30S ribosomal protein S19 Protein or peptide: 30S ribosomal protein S20 Ligand: ZINC ION Ligand: MAGNESIUM ION Ligand: SPERMIDINE Ligand: 1,4-DIAMINOBUTANEPutrescine Ligand: GUANOSINE-5'-DIPHOSPHATE Ligand: 2-[(3~{R},4~{S},5~{S},8~{S},9~{S},10~{S},11~{R},13~{S},14~{S},16~{S})-16-acetyloxy-4,8,10,14-tetramethyl-3,11-bis(oxidanyl)-1,2,3,4,5,6,7,9,11,12,13,15,16,17-tetradecahydrocyclopenta[a]phenanthren-17-yl]-5-cyclopentyl-pentanoic acid Ligand: POTASSIUM IONPotassium Ligand: water

Entire

Name: Staphylococcus aureus 70S ribosome with elongation factor G and fusidic acid cyclopentane

Components

Complex: Staphylococcus aureus 70S ribosome with elongation factor G and fusidic acid cyclopentane
- Protein or peptide: 50S ribosomal protein L28
- Protein or peptide: 50S ribosomal protein L29
- Protein or peptide: 50S ribosomal protein L30
- Protein or peptide: 50S ribosomal protein L31 type BRibosome
- Protein or peptide: 50S ribosomal protein L32
- Protein or peptide: 50S ribosomal protein L33 1Ribosome
- Protein or peptide: 50S ribosomal protein L34
- Protein or peptide: 50S ribosomal protein L35
- Protein or peptide: 50S ribosomal protein L36
- RNA: 23S ribosomal RNA
- RNA: 5S ribosomal RNA
- RNA: tRNATransfer RNA
- Protein or peptide: Elongation factor GEF-G
- Protein or peptide: 50S ribosomal protein L2
- Protein or peptide: 50S ribosomal protein L3
- Protein or peptide: 50S ribosomal protein L4
- Protein or peptide: 50S ribosomal protein L5
- Protein or peptide: 50S ribosomal protein L6
- Protein or peptide: 50S ribosomal protein L13
- Protein or peptide: 50S ribosomal protein L14
- Protein or peptide: 50S ribosomal protein L15
- Protein or peptide: 50S ribosomal protein L16
- Protein or peptide: 50S ribosomal protein L17
- Protein or peptide: 50S ribosomal protein L18
- Protein or peptide: 50S ribosomal protein L19
- Protein or peptide: 50S ribosomal protein L20
- Protein or peptide: 50S ribosomal protein L21
- Protein or peptide: 50S ribosomal protein L22
- Protein or peptide: 50S ribosomal protein L23
- Protein or peptide: 50S ribosomal protein L24
- Protein or peptide: 50S ribosomal protein L25
- Protein or peptide: 50S ribosomal protein L27
- RNA: 16S ribosomal RNA
- RNA: mRNAMessenger RNA
- Protein or peptide: 30S ribosomal protein S2
- Protein or peptide: 30S ribosomal protein S3
- Protein or peptide: 30S ribosomal protein S4
- Protein or peptide: 30S ribosomal protein S5
- Protein or peptide: 30S ribosomal protein S6
- Protein or peptide: 30S ribosomal protein S7
- Protein or peptide: 30S ribosomal protein S8
- Protein or peptide: 30S ribosomal protein S9
- Protein or peptide: 30S ribosomal protein S10
- Protein or peptide: 30S ribosomal protein S11
- Protein or peptide: 30S ribosomal protein S12
- Protein or peptide: 30S ribosomal protein S13
- Protein or peptide: 30S ribosomal protein S14 type ZRibosome
- Protein or peptide: 30S ribosomal protein S15
- Protein or peptide: 30S ribosomal protein S16
- Protein or peptide: 30S ribosomal protein S17
- Protein or peptide: 30S ribosomal protein S18
- Protein or peptide: 30S ribosomal protein S19
- Protein or peptide: 30S ribosomal protein S20
Ligand: ZINC ION
Ligand: MAGNESIUM ION
Ligand: SPERMIDINE
Ligand: 1,4-DIAMINOBUTANEPutrescine
Ligand: GUANOSINE-5'-DIPHOSPHATE
Ligand: 2-[(3~{R},4~{S},5~{S},8~{S},9~{S},10~{S},11~{R},13~{S},14~{S},16~{S})-16-acetyloxy-4,8,10,14-tetramethyl-3,11-bis(oxidanyl)-1,2,3,4,5,6,7,9,11,12,13,15,16,17-tetradecahydrocyclopenta[a]phenanthren-17-yl]-5-cyclopentyl-pentanoic acid
Ligand: POTASSIUM IONPotassium
Ligand: water

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Supramolecule #1: Staphylococcus aureus 70S ribosome with elongation factor G and f...

Supramolecule	Name: Staphylococcus aureus 70S ribosome with elongation factor G and fusidic acid cyclopentane type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#53
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 2.6 MDa

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Macromolecule #1: 50S ribosomal protein L28

Macromolecule	Name: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 6.995289 KDa
Sequence	String: MGKQCFVTGR KASTGNRRSH ALNSTKRRWN ANLQKVRILV DGKPKKVWVS ARALKSGKVT RV UniProtKB: Large ribosomal subunit protein bL28

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Macromolecule #2: 50S ribosomal protein L29

Macromolecule	Name: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 8.105266 KDa
Sequence	String: MKAKEIRDLT TSEIEEQIKS SKEELFNLRF QLATGQLEET ARIRTVRKTI ARLKTVARER EIEQSKANQ UniProtKB: Large ribosomal subunit protein uL29

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Macromolecule #3: 50S ribosomal protein L30

Macromolecule	Name: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 6.565683 KDa
Sequence	String: MAKLQITLTR SVIGRPETQR KTVEALGLKK TNSSVVVEDN PAIRGQINKV KHLVTVEEK UniProtKB: Large ribosomal subunit protein uL30

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Macromolecule #4: 50S ribosomal protein L31 type B

Macromolecule	Name: 50S ribosomal protein L31 type B / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 9.737912 KDa
Sequence	String: MKQGIHPEYH QVIFLDTTTN FKFLSGSTKT SSEMMEWEDG KEYPVIRLDI SSDSHPFYTG RQKFAAADGR VERFNKKFGL KSNN UniProtKB: Large ribosomal subunit protein bL31B

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Macromolecule #5: 50S ribosomal protein L32

Macromolecule	Name: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 6.500609 KDa
Sequence	String: MAVPKRRTSK TRKNKRRTHF KISVPGMTEC PNCGEYKLSH RVCKNCGSYN GEEVAAK UniProtKB: Large ribosomal subunit protein bL32

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Macromolecule #6: 50S ribosomal protein L33 1

Macromolecule	Name: 50S ribosomal protein L33 1 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 5.944937 KDa
Sequence	String: MRVNVTLACT ECGDRNYITT KNKRNNPERI EMKKYCPRLN KYTLHRETK UniProtKB: Large ribosomal subunit protein bL33A

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Macromolecule #7: 50S ribosomal protein L34

Macromolecule	Name: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 5.454642 KDa
Sequence	String: MVKRTYQPNK RKHSKVHGFR KRMSTKNGRK VLARRRRKGR KVLSA UniProtKB: Large ribosomal subunit protein bL34

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Macromolecule #8: 50S ribosomal protein L35

Macromolecule	Name: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 7.722368 KDa
Sequence	String: MPKMKTHRGA AKRVKRTASG QLKRSRAFTS HLFANKSTKQ KRQLRKARLV SKSDMKRVKQ LLAYKK UniProtKB: Large ribosomal subunit protein bL35

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Macromolecule #9: 50S ribosomal protein L36

Macromolecule	Name: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 4.318422 KDa
Sequence	String: MKVRPSVKPI CEKCKVIKRK GKVMVICENP KHKQRQG UniProtKB: Large ribosomal subunit protein bL36

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Macromolecule #13: Elongation factor G

Macromolecule	Name: Elongation factor G / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria)
Molecular weight	Theoretical: 76.699289 KDa
Recombinant expression	Organism: Escherichia coli BL21(DE3) (bacteria)
Sequence	String: MAREFSLEKT RNIGIMAHID AGKTTTTERI LYYTGRIHKI GETHEGASQM DWMEQEQDRG ITITSAATTA AWEGHRVNII DTPGHVDFT VEVERSLRVL DGAVTVLDAQ SGVEPQTETV WRQATTYGVP RIVFVNKMDK LGANFEYSVS TLHDRLQANA A PIQLPIGA ...String: MAREFSLEKT RNIGIMAHID AGKTTTTERI LYYTGRIHKI GETHEGASQM DWMEQEQDRG ITITSAATTA AWEGHRVNII DTPGHVDFT VEVERSLRVL DGAVTVLDAQ SGVEPQTETV WRQATTYGVP RIVFVNKMDK LGANFEYSVS TLHDRLQANA A PIQLPIGA EDEFEAIIDL VEMKCFKYTN DLGTEIEEIE IPEDHLDRAE EARASLIEAV AETSDELMEK YLGDEEISVS EL KEAIRQA TTNVEFYPVL CGTAFKNKGV QLMLDAVIDY LPSPLDVKPI IGHRASNPEE EVIAKADDSA EFAALAFKVM TDP YVGKLT FFRVYSGTMT SGSYVKNSTK GKRERVGRLL QMHANSRQEI DTVYSGDIAA AVGLKDTGTG DTLCGEKNDI ILES MEFPE PVIHLSVEPK SKADQDKMTQ ALVKLQEEDP TFHAHTDEET GQVIIGGMGE LHLDILVDRM KKEFNVECNV GAPMV SYRE TFKSSAQVQG KFSRQSGGRG QYGDVHIEFT PNETGAGFEF ENAIVGGVVP REYIPSVEAG LKDAMENGVL AGYPLI DVK AKLYDGSYHD VDSSEMAFKI AASLALKEAA KKCDPVILEP MMKVTIEMPE EYMGDIMGDV TSRRGRVDGM EPRGNAQ VV NAYVPLSEMF GYATSLRSNT QGRGTYTMYF DHYAEVPKSI AEDIIKKNKG E UniProtKB: Elongation factor G

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Macromolecule #14: 50S ribosomal protein L2

Macromolecule	Name: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 30.217164 KDa
Sequence	String: MAIKKYKPIT NGRRNMTSLD FAEITKTTPE KSLLKPLPKK AGRNNQGKLT VRHHGGGHKR QYRVIDFKRN KDGINAKVDS IQYDPNRSA NIALVVYADG EKRYIIAPKG LEVGQIVESG AEADIKVGNA LPLQNIPVGT VVHNIELKPG KGGQIARSAG A SAQVLGKE ...String: MAIKKYKPIT NGRRNMTSLD FAEITKTTPE KSLLKPLPKK AGRNNQGKLT VRHHGGGHKR QYRVIDFKRN KDGINAKVDS IQYDPNRSA NIALVVYADG EKRYIIAPKG LEVGQIVESG AEADIKVGNA LPLQNIPVGT VVHNIELKPG KGGQIARSAG A SAQVLGKE GKYVLIRLRS GEVRMILSTC RATIGQVGNL QHELVNVGKA GRSRWKGIRP TVRGSVMNPN DHPHGGGEGR AP IGRPSPM SPWGKPTLGK KTRRGKKSSD KLIVRGRKKK UniProtKB: Large ribosomal subunit protein uL2

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Macromolecule #15: 50S ribosomal protein L3

Macromolecule	Name: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 23.760256 KDa
Sequence	String: MTKGILGRKI GMTQVFGENG ELIPVTVVEA KENVVLQKKT VEVDGYNAIQ VGFEDKKAYK KDAKSNKYAN KPAEGHAKKA DAAPKRFIR EFRNVDVDAY EVGQEVSVDT FVAGDVIDVT GVSKGKGFQG AIKRHGQSRG PMSHGSHFHR APGSVGMASD A SRVFKGQK ...String: MTKGILGRKI GMTQVFGENG ELIPVTVVEA KENVVLQKKT VEVDGYNAIQ VGFEDKKAYK KDAKSNKYAN KPAEGHAKKA DAAPKRFIR EFRNVDVDAY EVGQEVSVDT FVAGDVIDVT GVSKGKGFQG AIKRHGQSRG PMSHGSHFHR APGSVGMASD A SRVFKGQK MPGRMGGNTV TVQNLEVVQV DTENKVILVK GNVPGPKKGL VEIRTSIKKG NK UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #16: 50S ribosomal protein L4

Macromolecule	Name: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 22.495697 KDa
Sequence	String: MANYDVLKLD GTKSGSIELS DAVFGIEPNN SVLFEAINLQ RASLRQGTHA VKNRSAVSGG GRKPWKQKGT GRARQGTIRA PQWRGGGIV FGPTPRSYAY KMPKKMRRLA LRSALSFKAQ ENGLTVVDAF NFEAPKTKEF KNVLSTLEQP KKVLVVTENE D VNVELSAR ...String: MANYDVLKLD GTKSGSIELS DAVFGIEPNN SVLFEAINLQ RASLRQGTHA VKNRSAVSGG GRKPWKQKGT GRARQGTIRA PQWRGGGIV FGPTPRSYAY KMPKKMRRLA LRSALSFKAQ ENGLTVVDAF NFEAPKTKEF KNVLSTLEQP KKVLVVTENE D VNVELSAR NIPGVQVTTA QGLNVLDITN ADSLVITEAA AKKVEEVLG UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #17: 50S ribosomal protein L5

Macromolecule	Name: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 20.296637 KDa
Sequence	String: MNRLKEKFNT EVTENLMKKF NYSSVMEVPK IDKIVVNMGV GDAVQNSKVL DNAVEELELI TGQKPLVTKA KKSIATFRLR EGMPIGAKV TLRGERMYEF LDKLISVSLP RVRDFQGVSK KAFDGRGNYT LGVKEQLIFP EIDYDKVSKV RGMDIVIVTT A NTDEEARE LLANFGMPFR K UniProtKB: Large ribosomal subunit protein uL5

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Macromolecule #18: 50S ribosomal protein L6

Macromolecule	Name: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 19.81858 KDa
Sequence	String: MSRVGKKIID IPSDVTVTFD GNHVTVKGPK GELSRTLNER MTFKQEENTI EVVRPSDSKE DRTNHGTTRA LLNNMVQGVS QGYVKVLEL VGVGYRAQMQ GKDLILNVGY SHPVEIKAEE NITFSVEKNT VVKVEGISKE QVGALASNIR SVRPPEPYKG K GIRYQGEY VRRKEGKTGK UniProtKB: Large ribosomal subunit protein uL6

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Macromolecule #19: 50S ribosomal protein L13

Macromolecule	Name: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 16.359427 KDa
Sequence	String: MRQTFMANES NIERKWYVID AEGQTLGRLS SEVASILRGK NKVTYTPHVD TGDYVIVINA SKIEFTGNKE TDKVYYRHSN HPGGIKSIT AGELRRTNPE RLIENSIKGM LPSTRLGEKQ GKKLFVYGGA EHPHAAQQPE NYELRG UniProtKB: Large ribosomal subunit protein uL13

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Macromolecule #20: 50S ribosomal protein L14

Macromolecule	Name: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 13.157342 KDa
Sequence	String: MIQQETRLKV ADNSGAREVL TIKVLGGSGR KTANIGDVIV CTVKNATPGG VVKKGDVVKA VIVRTKSGVR RNDGSYIKFD ENACVIIRD DKGPRGTRIF GPVARELREG NFMKIVSLAP EVL UniProtKB: Large ribosomal subunit protein uL14

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Macromolecule #21: 50S ribosomal protein L15

Macromolecule	Name: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 15.62889 KDa
Sequence	String: MKLHELKPAE GSRKERNRVG RGVATGNGKT SGRGHKGQKA RSGGGVRPGF EGGQLPLFRR LPKRGFTNIN RKEYAIVNLD QLNKFEDGT EVTPALLVES GVVKNEKSGI KILGNGSLDK KLTVKAHKFS ASAAEAIDAK GGAHEVI UniProtKB: Large ribosomal subunit protein uL15

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Macromolecule #22: 50S ribosomal protein L16

Macromolecule	Name: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 16.274049 KDa
Sequence	String: MLLPKRVKYR RQHRPKTTGR SKGGNYVTFG EFGLQATTTS WITSRQIESA RIAMTRYMKR GGKVWIKIFP HTPYTKKPLE VRMGAGKGA VEGWIAVVKP GRILFEVAGV SEEVAREALR LASHKLPVKT KFVKREELGG ETNES UniProtKB: Large ribosomal subunit protein uL16

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Macromolecule #23: 50S ribosomal protein L17

Macromolecule	Name: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 13.771773 KDa
Sequence	String: MGYRKLGRTS DQRKAMLRDL ATSLIISERI ETTEARAKEV RSVVEKLITL GKKGDLASRR NAAKTLRNVE ILNEDETTQT ALQKLFGEI AERYTERQGG YTRILKQGPR RGDGAESVII ELV UniProtKB: Large ribosomal subunit protein bL17

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Macromolecule #24: 50S ribosomal protein L18

Macromolecule	Name: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 13.124093 KDa
Sequence	String: MISKIDKNKV RLKRHARVRT NLSGTAEKPR LNVYRSNKHI YAQIIDDNKG VTLAQASSKD SDIATTATKV ELATKVGEAI AKKAADKGI KEIVFDRGGY LYHGRVKALA EAARESGLEF UniProtKB: Large ribosomal subunit protein uL18

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Macromolecule #25: 50S ribosomal protein L19

Macromolecule	Name: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 13.392771 KDa
Sequence	String: MTNHKLIEAV TKSQLRTDLP SFRPGDTLRV HVRIIEGTRE RIQVFEGVVI KRRGGGVSET FTVRKISSGV GVERTFPLHT PKIEKIEVK RRGKVRRAKL YYLRSLRGKA ARIQEIR UniProtKB: Large ribosomal subunit protein bL19

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Macromolecule #26: 50S ribosomal protein L20

Macromolecule	Name: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 13.720295 KDa
Sequence	String: MPRVKGGTVT RARRKKTIKL AKGYFGSKHT LYKVAKQQVM KSGQYAFRDR RQRKRDFRKL WITRINAAAR QHEMSYSRLM NGLKKAGID INRKMLSEIA ISDEKAFAQL VTKAKDALK UniProtKB: Large ribosomal subunit protein bL20

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Macromolecule #27: 50S ribosomal protein L21

Macromolecule	Name: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 11.354081 KDa
Sequence	String: MFAIIETGGK QIKVEEGQEI FVEKLDVNEG DTFTFDKVLF VGGDSVKVGA PTVEGATVTA TVNKQGRGKK ITVFTYKRRK NSKRKKGHR QPYTKLTIDK INA UniProtKB: Large ribosomal subunit protein bL21

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Macromolecule #28: 50S ribosomal protein L22

Macromolecule	Name: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 12.857922 KDa
Sequence	String: MEAKAVARTI RIAPRKVRLV LDLIRGKNAA EAIAILKLTN KASSPVIEKV LMSALANAEH NYDMNTDELV VKEAYANEGP TLKRFRPRA QGRASAINKR TSHITIVVSD GKEEAKEA UniProtKB: Large ribosomal subunit protein uL22

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Macromolecule #29: 50S ribosomal protein L23

Macromolecule	Name: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 10.625398 KDa
Sequence	String: MEARDILKRP VITEKSSEAM AEDKYTFDVD TRVNKTQVKM AVEEIFNVKV ASVNIMNYKP KKKRMGRYQG YTNKRRKAIV TLKEGSIDL FN UniProtKB: Large ribosomal subunit protein uL23

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Macromolecule #30: 50S ribosomal protein L24

Macromolecule	Name: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 11.561504 KDa
Sequence	String: MHIKKGDNVK VIAGKDKGKE GKVIATLPKK DRVVVEGVNI MKKHQKPTQL NPEGGILETE AAIHVSNVQL LDPKTNEPTR VGYKFVDGK KVRIAKKSGE EIKSNN UniProtKB: Large ribosomal subunit protein uL24

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Macromolecule #31: 50S ribosomal protein L25

Macromolecule	Name: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 23.810609 KDa
Sequence	String: MASLKSIIRQ GKQTRSDLKQ LRKSGKVPAV VYGYGTKNVS VKVDEVEFIK VIREVGRNGV IELGVGSKTI KVMVADYQFD PLKNQITHI DFLAINMSEE RTVEVPVQLV GEAVGAKEGG VVEQPLFNLE VTATPDNIPE AIEVDITELN INDSLTVADV K VTGDFKIE ...String: MASLKSIIRQ GKQTRSDLKQ LRKSGKVPAV VYGYGTKNVS VKVDEVEFIK VIREVGRNGV IELGVGSKTI KVMVADYQFD PLKNQITHI DFLAINMSEE RTVEVPVQLV GEAVGAKEGG VVEQPLFNLE VTATPDNIPE AIEVDITELN INDSLTVADV K VTGDFKIE NDSAESVVTV VAPTEEPTEE EIEAMEGEQQ TEEPEVVGES KEDEEKTEE UniProtKB: Large ribosomal subunit protein bL25

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Macromolecule #32: 50S ribosomal protein L27

Macromolecule	Name: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 10.334798 KDa
Sequence	String: MLKLNLQFFA SKKGVSSTKN GRDSESKRLG AKRADGQFVT GGSILYRQRG TKIYPGENVG RGGDDTLFAK IDGVVKFERK GRDKKQVSV YAVAE UniProtKB: Large ribosomal subunit protein bL27

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Macromolecule #35: 30S ribosomal protein S2

Macromolecule	Name: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 29.136369 KDa
Sequence	String: MAVISMKQLL EAGVHFGHQT RRWNPKMKKY IFTERNGIYI IDLQKTVKKV DEAYNFLKQV SEDGGQVLFV GTKKQAQESV KSEAERAGQ FYINQRWLGG LLTNYKTISK RIKRISEIEK MEEDGLFEVL PKKEVVELKK EYDRLIKFLG GIRDMKSMPQ A LFVVDPRK ...String: MAVISMKQLL EAGVHFGHQT RRWNPKMKKY IFTERNGIYI IDLQKTVKKV DEAYNFLKQV SEDGGQVLFV GTKKQAQESV KSEAERAGQ FYINQRWLGG LLTNYKTISK RIKRISEIEK MEEDGLFEVL PKKEVVELKK EYDRLIKFLG GIRDMKSMPQ A LFVVDPRK ERNAIAEARK LNIPIVGIVD TNCDPDEIDY VIPANDDAIR AVKLLTAKMA DAILEGQQGV SNEEVAAEQN ID LDEKEKS EETEATEE UniProtKB: Small ribosomal subunit protein uS2

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Macromolecule #36: 30S ribosomal protein S3

Macromolecule	Name: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 24.143867 KDa
Sequence	String: MGQKINPIGL RVGIIRDWEA KWYAEKDFAS LLHEDLKIRK FIDNELKEAS VSHVEIERAA NRINIAIHTG KPGMVIGKGG SEIEKLRNK LNALTDKKVH INVIEIKKVD LDARLVAENI ARQLENRASF RRVQKQAITR AMKLGAKGIK TQVSGRLGGA D IARAEQYS ...String: MGQKINPIGL RVGIIRDWEA KWYAEKDFAS LLHEDLKIRK FIDNELKEAS VSHVEIERAA NRINIAIHTG KPGMVIGKGG SEIEKLRNK LNALTDKKVH INVIEIKKVD LDARLVAENI ARQLENRASF RRVQKQAITR AMKLGAKGIK TQVSGRLGGA D IARAEQYS EGTVPLHTLR ADIDYAHAEA DTTYGKLGVK VWIYRGEVLP TKNTSGGGK UniProtKB: Small ribosomal subunit protein uS3

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Macromolecule #37: 30S ribosomal protein S4

Macromolecule	Name: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 23.051416 KDa
Sequence	String: MARFRGSNWK KSRRLGISLS GTGKELEKRP YAPGQHGPNQ RKKLSEYGLQ LREKQKLRYL YGMTERQFRN TFDIAGKKFG VHGENFMIL LASRLDAVVY SLGLARTRRQ ARQLVNHGHI LVDGKRVDIP SYSVKPGQTI SVREKSQKLN IIVESVEINN F VPEYLNFD ...String: MARFRGSNWK KSRRLGISLS GTGKELEKRP YAPGQHGPNQ RKKLSEYGLQ LREKQKLRYL YGMTERQFRN TFDIAGKKFG VHGENFMIL LASRLDAVVY SLGLARTRRQ ARQLVNHGHI LVDGKRVDIP SYSVKPGQTI SVREKSQKLN IIVESVEINN F VPEYLNFD ADSLTGTFVR LPERSELPAE INEQLIVEYY SR UniProtKB: Small ribosomal subunit protein uS4

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Macromolecule #38: 30S ribosomal protein S5

Macromolecule	Name: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 17.770512 KDa
Sequence	String: MARREEETKE FEERVVTINR VAKVVKGGRR FRFTALVVVG DKNGRVGFGT GKAQEVPEAI KKAVEAAKKD LVVVPRVEGT TPHTITGRY GSGSVFMKPA APGTGVIAGG PVRAVLELAG ITDILSKSLG SNTPINMVRA TIDGLQNLKN AEDVAKLRGK T VEELYN UniProtKB: Small ribosomal subunit protein uS5

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Macromolecule #39: 30S ribosomal protein S6

Macromolecule	Name: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 11.613146 KDa
Sequence	String: MRTYEVMYIV RPNIEEDAKK ALVERFNGIL ATEGAEVLEA KDWGKRRLAY EINDFKDGFY NIVRVKSDNN KATDEFQRLA KISDDIIRY MVIREDEDK UniProtKB: Small ribosomal subunit protein bS6

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Macromolecule #40: 30S ribosomal protein S7

Macromolecule	Name: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 17.826555 KDa
Sequence	String: MPRKGSVPKR DVLPDPIHNS KLVTKLINKI MLDGKRGTAQ RILYSAFDLV EQRSGRDALE VFEEAINNIM PVLEVKARRV GGSNYQVPV EVRPERRTTL GLRWLVNYAR LRGEKTMEDR LANEILDAAN NTGGAVKKRE DTHKMAEANK AFAHYRW UniProtKB: Small ribosomal subunit protein uS7

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Macromolecule #41: 30S ribosomal protein S8

Macromolecule	Name: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 14.854315 KDa
Sequence	String: MTMTDPIADM LTRVRNANMV RHEKLELPAS NIKKEIAEIL KSEGFIKNVE YVEDDKQGVL RLFLKYGQND ERVITGLKRI SKPGLRVYA KASEMPKVLN GLGIALVSTS EGVITDKEAR KRNVGGEIIA YVW UniProtKB: Small ribosomal subunit protein uS8

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Macromolecule #42: 30S ribosomal protein S9

Macromolecule	Name: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 14.856987 KDa
Sequence	String: MTLAQVEYRG TGRRKNSVAR VRLVPGEGNI TVNNRDVREY LPFESLILDL NQPFDVTETK GNYDVLVNVH GGGFTGQAQA IRHGIARAL LEADPEYRGS LKRAGLLTRD PRMKERKKPG LKAARRSPQF SKR UniProtKB: Small ribosomal subunit protein uS9

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Macromolecule #43: 30S ribosomal protein S10

Macromolecule	Name: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 11.598503 KDa
Sequence	String: MAKQKIRIRL KAYDHRVIDQ SAEKIVETAK RSGADVSGPI PLPTEKSVYT IIRAVHKYKD SREQFEQRTH KRLIDIVNPT PKTVDALMG LNLPSGVDIE IKL UniProtKB: Small ribosomal subunit protein uS10

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Macromolecule #44: 30S ribosomal protein S11

Macromolecule	Name: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 13.907978 KDa
Sequence	String: MARKQVSRKR RVKKNIENGV AHIRSTFNNT IVTITDEFGN ALSWSSAGAL GFKGSKKSTP FAAQMASETA SKSAMEHGLK TVEVTVKGP GPGRESAIRA LQSAGLEVTA IRDVTPVPHN GCRPPKRRRV UniProtKB: Small ribosomal subunit protein uS11

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Macromolecule #45: 30S ribosomal protein S12

Macromolecule	Name: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 15.32087 KDa
Sequence	String: MPTINQLVRK PRQSKIKKSD SPALNKGFNS KKKKFTDLNS PQKRGVCTRV GTMTPKKPNS ALRKYARVRL SNNIEINAYI PGIGHNLQE HSVVLVRGGR VKDLPGVRYH IVRGALDTSG VDGRRQGRSL YGTKKPKN UniProtKB: Small ribosomal subunit protein uS12

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Macromolecule #46: 30S ribosomal protein S13

Macromolecule	Name: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 13.747919 KDa
Sequence	String: MARIAGVDIP REKRVVISLT YIYGIGTSTA QKILEEANVS ADTRVKDLTD DELGRIREVV DGYKVEGDLR RETNLNIKRL MEISSYRGI RHRRGLPVRG QKTKNNARTR KGPVKTVANK KK UniProtKB: Small ribosomal subunit protein uS13

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Macromolecule #47: 30S ribosomal protein S14 type Z

Macromolecule	Name: 30S ribosomal protein S14 type Z / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 7.317769 KDa
Sequence	String: MAKTSMVAKQ QKKQKYAVRE YTRCERCGRP HSVYRKFKLC RICFRELAYK GQIPGVRKAS W UniProtKB: Small ribosomal subunit protein uS14B

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Macromolecule #48: 30S ribosomal protein S15

Macromolecule	Name: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 10.63433 KDa
Sequence	String: MAISQERKNE IIKEYRVHET DTGSPEVQIA VLTAEINAVN EHLRTHKKDH HSRRGLLKMV GRRRHLLNYL RSKDIQRYRE LIKSLGIRR UniProtKB: Small ribosomal subunit protein uS15

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Macromolecule #49: 30S ribosomal protein S16

Macromolecule	Name: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 10.253886 KDa
Sequence	String: MAVKIRLTRL GSKRNPFYRI VVADARSPRD GRIIEQIGTY NPTSANAPEI KVDEALALKW LNDGAKPTDT VHNILSKEGI MKKFDEQKK AK UniProtKB: Small ribosomal subunit protein bS16

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Macromolecule #50: 30S ribosomal protein S17

Macromolecule	Name: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 10.196888 KDa
Sequence	String: MSERNDRKVY VGKVVSDKMD KTITVLVETY KTHKLYGKRV KYSKKYKTHD ENNSAKLGDI VKIQETRPLS ATKRFRLVEI VEESVII UniProtKB: Small ribosomal subunit protein uS17

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Macromolecule #51: 30S ribosomal protein S18

Macromolecule	Name: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 9.332018 KDa
Sequence	String: MAGGPRRGGR RRKKVCYFTA NGITHIDYKD TELLKRFISE RGKILPRRVT GTSAKYQRML TTAIKRSRHM ALLPYVKEEQ UniProtKB: Small ribosomal subunit protein bS18

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Macromolecule #52: 30S ribosomal protein S19

Macromolecule	Name: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 10.639309 KDa
Sequence	String: MARSIKKGPF VDEHLMKKVE AQEGSEKKQV IKTWSRRSTI FPNFIGHTFA VYDGRKHVPV YVTEDMVGHK LGEFAPTRTF KGHVADDKK TRR UniProtKB: Small ribosomal subunit protein uS19

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Macromolecule #53: 30S ribosomal protein S20

Macromolecule	Name: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 9.039472 KDa
Sequence	String: MANIKSAIKR VKTTEKAEAR NISQKSAMRT AVKNAKTAVS NNADNKNELV SLAVKLVDKA AQSNLIHSNK ADRIKSQLMT ANK UniProtKB: Small ribosomal subunit protein bS20

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Macromolecule #10: 23S ribosomal RNA

Macromolecule	Name: 23S ribosomal RNA / type: rna / ID: 10 / Number of copies: 1
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 946.782688 KDa
Sequence	String: GAUUAAGUUA UUAAGGGCGC ACGGUGGAUG CCUUGGCACU AGAAGCCGAU GAAGGACGUU ACUAACGACG AUAUGCUUUG GGGAGCUGU AAGUAAGCUU UGAUCCAGAG AUUUCCGAAU GGGGAAACCC AGCAUGAGUU AUGUCAUGUU AUCGAUAUGU G AAUACAUA ...String: GAUUAAGUUA UUAAGGGCGC ACGGUGGAUG CCUUGGCACU AGAAGCCGAU GAAGGACGUU ACUAACGACG AUAUGCUUUG GGGAGCUGU AAGUAAGCUU UGAUCCAGAG AUUUCCGAAU GGGGAAACCC AGCAUGAGUU AUGUCAUGUU AUCGAUAUGU G AAUACAUA GCAUAUCAGA AGGCACACCC GGAGAACUGA AACAUCUUAG UACCCGGAGG AAGAGAAAGA AAAUUCGAUU CC CUUAGUA GCGGCGAGCG AAACGGGAAG AGCCCAAACC AACAAGCUUG CUUGUUGGGG UUGUAGGACA CUCUAUACGG AGU UACAAA GGACGACAUU AGACGAAUCA UCUGGAAAGA UGAAUCAAAG AAGGUAAUAA UCCUGUAGUC GAAAAUGUUG UCUC UCUUG AGUGGAUCCU GAGUACGACG GAGCACGUGA AAUUCCGUCG GAAUCUGGGA GGACCAUCUC CUAAGGCUAA AUACU CUCU AGUGACCGAU AGUGAACCAG UACCGUGAGG GAAAGGUGAA AAGCACCCCG GAAGGGGAGU GAAAUAGAAC CUGAAA CCG UGUGCUUACA AGUAGUCAGA GCCCGUUAAU GGGUGAUGGC GUGCCUUUUG UAGAAUGAAC CGGCGAGUUA CGAUUUG AU GCAAGGUUAA GCAGUAAAUG UGGAGCCGUA GCGAAAGCGA GUCUGAAUAG GGCGUUUAGU AUUUGGUCGU AGACCCGA A ACCAGGUGAU CUACCCUUGG UCAGGUUGAA GUUCAGGUAA CACUGAAUGG AGGACCGAAC CGACUUACGU (5MU)GAAA AGUG AGCGGAUGAA CUGAGGGUAG CGGAGAAAUU CCAAUCGAAC CUGGAGAUAG CUGGUUCUCU CCGAAAUAGC UUUAGG GCU AGCCUCAAGU GAUGAUUAUU GGAGGUAGAG CACUGUUUGG ACGAGGGGCC CCUCUCGGGU UACCGAAUUC AGACAAA CU CCGAAUGCCA AUUAAUUUAA CUUGGGAGUC AGAACAUGGG UGAUAAGGUC CGUGUUCGAA AGGGAAACAG CCCAGACC A CCAGCUAAGG UCCCAAAAUA UAUGUUAAGU GGAAAAGGAU GUGGCGUUGC CCAGACAACU AGGAUGUUGG CUUAGAAGC AGCCAUCAUU UAAAGAGUGC GUAAUAGCUC ACUAGUCGAG UGACACUGCG CCGAAAAUGU ACCGGGGCUA AACAUAUUAC CGAAGCUGU GGAUUGUCCU UUGGACAAUG GUAGGAGAGC GUUCUAAGGG CGUUGAAGCA UGAUCGUAAG GACAUGUGGA G CGCUUAGA AGUGAGAAUG CCGGUGUGAG UAGCGAAAGA CGGGUGAGAA UCCCGUCCAC CGAUUGACUA AGGUUUCCAG AG GAAGGCU CGUCCGCUCU GGGUUAGUCG GGUCCUAAGC UGAGGCCGAC AGGCGUAGGC GAUGGAUAAC AGGUUGAUAU UCC UGUACC ACCUAUAAUC GUUUUAAUCG AUGGGGGGAC GCAGUAGGAU AGGCGAAGCG UGCGAUUGGA UUGCACGUCU AAGC AGUAA GGCUGAGUAU UAGGCAAAUC CGGUACUCGU UAAGGCUGAG CUGUGAUGGG GAGAAGACAU UGUGUCUUCG AGUCG UUGA UUUCACACUG CCGAGAAAAG CCUCUAGAUA GAAAAUAGGU GCCCGUACCG CAAACCGACA CAGGUAGUCA AGAUGA GAA UUCUAAGGUG AGCGAGCGAA CUCUCGUUAA GGAACUCGGC AAAAUGACCC CGUAACUUCG GGAGAAGGGG UGCUCUU UA GGGUUAACGC CCAGAAGAGC CGCAGUGAAU AGGCCCAAGC GACUGUUUAU CAAAAACACA GGUCUCUGCU AAACCGUA A GGUGAUGUAU AGGGGCUGAC GCCUGCCCGG UGCUGGAAGG UUAAGAGGAG UGGUUAGCUU CUGCGAAGCU ACGAAUCGA AGCCCCAGUA AACGGCGGCC GUAAC(3TD)AUAA CGGUCCUAAG GUAGCGAAA(5MU) UCCUUGUCGG GUAAGUUCCG AC CCGCACG AAAGGCGUAA CGAUUUGGGC ACUGUCUCAA CGAGAGACUC GGUGAAAUCA UAGUACCUGU GAAGAUGCAG GUU ACCCGC GACAGGACGG AAAGACCCCG UGGAGCUUUA CUGUAGCCUG AUAUUGAAAU UCGGCACAGC UUGUACAGGA UAGG UAGGA GCCUUUGAAA CGUGAGCGCU AGCUUACGUG GAGGCGCUGG UGGGAUACUA CCCUAGCUGU GUUGGCUUUC UAACC CGCA CCACUUAUCG UGGUGGGAGA CAGUGUCAGG CGGGCAGUUU GACUG(OMG)GGCG GUCGCCUCCU AAAAGGUAAC GG AGGCGCU CAAAGGUUCC CUCAGAAUGG UUGGAAAUCA UUCAUAGAGU GUAAAGGCAU AAGGGAGCUU GACUGCGAGA CCU ACAAGU CGAGCAGGGU CGAAAGACGG ACUUAGUGAU CCGGUGGUUC CGCAUGGAAG GGCCAUCGCU CAACGGAUAA AAGC UACCC CG(2MG)GGA(H2U)AAC AGGCUUAUCU CCCCCAAGAG UUCACAUCGA CGGGGAGGUU UGGCACCUCG (2MA)UG UCGGCU CAUCGCAUCC UGGGGCUGUA GUCGGUCCCA AGGGUUGGGC UGUUCGCCCA UUAAAGCGGU ACGCGAGCUG GGUU CAGAA CGUCGUGAGA CAGUUCGGUC CCUAUCCGUC GUGGGCGUAG GAAAUUUGAG AGGAGCUGUC CUUAGUACGA GAGGA CCGG GAUGGACAUA CCUCUGGUGU ACCAGUUGUC GUGCCAACGG CAUAGCUGGG UAGCUAUGUG UGGACGGGAU AAGUGC UGA AAGCAUCUAA GCAUGAAGCC CCCCUCAAGA UGAGAUUUCC CAACUUCGGU UAUAAGAUCC CUCAAAGAUG AUGAGGU UA AUAGGUUCGA GGUGGAAGCA UGGUGACAUG UGGAGCUGAC GAAUACUAAU CGAUCGAAGA CUUAAUCAA GENBANK: GENBANK: CP000253.1

+
Macromolecule #11: 5S ribosomal RNA

Macromolecule	Name: 5S ribosomal RNA / type: rna / ID: 11 / Number of copies: 1
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 36.974945 KDa
Sequence	String: UCUGGUGACU AUAGCAAGGA GGUCACACCU GUUCCCAUGC CGAACACAGA AGUUAAGCUC CUUAGCGUCG AUGGUAGUCG AACUUACGU UCCGCUAGAG UAGAACGUUG CCAGGC GENBANK: GENBANK: CP000253.1

+
Macromolecule #12: tRNA

Macromolecule	Name: tRNA / type: rna / ID: 12 / Number of copies: 2
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 24.802785 KDa
Sequence	String: CGCGGGGUGG AGCAGCCUGG UAGCUCGUCG GGCUCAUAAC CCGAAGGUCG UCGGUUCAAA UCCGGCCCCC GCAACCA GENBANK: GENBANK: AP023205.1

+
Macromolecule #33: 16S ribosomal RNA

Macromolecule	Name: 16S ribosomal RNA / type: rna / ID: 33 / Number of copies: 1
Source (natural)	Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria) Strain: 8325-4
Molecular weight	Theoretical: 503.117219 KDa
Sequence	String: UUUUAUGGAG AGUUUGAUCC UGGCUCAGGA UGAACGCUGG CGGCGUGCCU AAUACAUGCA AGUCGAGCGA ACGGACGAGA AGCUUGCUU CUCUGAUGUU AGCGGCGGAC GGGUGAGUAA CACGUGGAUA ACCUACCUAU AAGACUGGGA UAACUUCGGG A AACCGGAG ...String: UUUUAUGGAG AGUUUGAUCC UGGCUCAGGA UGAACGCUGG CGGCGUGCCU AAUACAUGCA AGUCGAGCGA ACGGACGAGA AGCUUGCUU CUCUGAUGUU AGCGGCGGAC GGGUGAGUAA CACGUGGAUA ACCUACCUAU AAGACUGGGA UAACUUCGGG A AACCGGAG CUAAUACCGG AUAAUAUUUU GAACCGCAUG GUUCAAAAGU GAAAGACGGU CUUGCUGUCA CUUAUAGAUG GA UCCGCGC UGCAUUAGCU AGUUGGUAAG GUAACGGCUU ACCAAGGCAA CGAUGCAUAG CCGACCUGAG AGGGUGAUCG GCC ACACUG GAACUGAGAC ACGGUCCAGA CUCCUACGGG AGGCAGCAGU AGGGAAUCUU CCGCAAUGGG CGAAAGCCUG ACGG AGCAA CGCCGCGUGA GUGAUGAAGG UCUUCGGAUC GUAAAACUCU GUUAUUAGGG AAGAACAUAU GUGUAAGUAA CUGUG CACA UCUUGACGGU ACCUAAUCAG AAAGCCACGG CUAACUACGU GCCAGCAGCC (G7M)CGGUAAUAC GUAGGUGGCA AG CGUUAUC CGGAAUUAUU GGGCGUAAAG CGCGCGUAGG CGGUUUUUUA AGUCUGAUGU GAAAGCCCAC GGCUCAACCG UGG AGGGUC AUUGGAAACU GGAAAACUUG AGUGCAGAAG AGGAAAGUGG AAUUCCAUGU GUAGCGGUGA AAUGCGCAGA GAUA UGGAG GAACACCAGU GGCGAAGGCG ACUUUCUGGU CUGUAACUGA CGCUGAUGUG CGAAAGCGUG GGGAUCAAAC AGGAU UAGA UACCCUGGUA GUCCACGCCG UAAACGAUGA GUGCUAAGUG UUAGGGGGUU UCCGCCCCUU AGUGCUGCAG CUAACG CAU UAAGCACUCC GCCUGGGGAG UACGACCGCA AGGUUGAAAC UCAAAGGAAU UGACGGGGAC CCGCACAAGC GGUGGAG CA UGUGGUUUAA UUCGAA(2MG)(5MC)AA CGCGAAGAAC CUUACCAAAU CUUGACAUCC UUUGACAACU CUAGAGAUAG AGCCUUCCC CUUCGGGGGA CAAAGUGACA GGUGGUGCAU GGUUGUCGUC AGCUCGUGUC GUGAGAUGUU GGGUUAAGUC C CGCAACGA GCGCAACCCU UAAGCUUAGU UGCCAUCAUU AAGUUGGGCA CUCUAAGUUG ACUGCCGGUG ACAAACCGGA GG AAGGUGG GGAUGACGUC AAAUCAUCAU GCCCCUUAUG AUUUGGGCUA CACACGUGCU ACAAUGGACA AUACAAAGGG CAG CGAAAC CGCGAGGUCA AGCAAAUCCC AUAAAGUUGU UCUCAGUUCG GAUUGUAGUC UGCAACUCGA CUACAUGAAG CUGG AAUCG CUAGUAAUCG UAGAUCAGCA UGCUACGGUG AAUACGUUCC CGGGUCUUGU ACACACCG(4OC)C CGUCACACCA C GAGAGUUU GUAACACCCG AAGCCGGUGG AGUAACCUUU UAGGAGCUAG CCGUCGAAGG UGGGACAAAU GAUUGGGGUG AA GUCG(UR3)AA CAAGGUAGCC GUAUCGG(MA6)(MA6)G GUGCGGCUGG AUCACCUCCU UUCU GENBANK: GENBANK: CP000253.1

+
Macromolecule #34: mRNA

Macromolecule	Name: mRNA / type: rna / ID: 34 / Number of copies: 1
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 7.889855 KDa
Sequence	String: GGCAAGGAGG UAAAAAUGGC AAAA

+
Macromolecule #54: ZINC ION

+
Macromolecule #55: MAGNESIUM ION

+
Macromolecule #56: SPERMIDINE

Macromolecule	Name: SPERMIDINE / type: ligand / ID: 56 / Number of copies: 1 / Formula: SPD
Molecular weight	Theoretical: 145.246 Da
Chemical component information	ChemComp-SPD: SPERMIDINE / Spermidine

+
Macromolecule #57: 1,4-DIAMINOBUTANE

Macromolecule	Name: 1,4-DIAMINOBUTANE / type: ligand / ID: 57 / Number of copies: 1 / Formula: PUT
Molecular weight	Theoretical: 88.151 Da
Chemical component information	ChemComp-PUT: 1,4-DIAMINOBUTANE / Putrescine

+
Macromolecule #58: GUANOSINE-5'-DIPHOSPHATE

Macromolecule	Name: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 58 / Number of copies: 1 / Formula: GDP
Molecular weight	Theoretical: 443.201 Da
Chemical component information	ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

+
Macromolecule #59: 2-[(3~{R},4~{S},5~{S},8~{S},9~{S},10~{S},11~{R},13~{S},14~{S},16~...

Macromolecule

Name: 2-[(3~{R},4~{S},5~{S},8~{S},9~{S},10~{S},11~{R},13~{S},14~{S},16~{S})-16-acetyloxy-4,8,10,14-tetramethyl-3,11-bis(oxidanyl)-1,2,3,4,5,6,7,9,11,12,13,15,16,17-tetradecahydrocyclopenta[a] ...
type: ligand / ID: 59 / Number of copies: 1 / Formula: WUX

Molecular weight

Theoretical: 546.778 Da

+
Macromolecule #60: POTASSIUM ION

+
Macromolecule #61: water

Macromolecule	Name: water / type: ligand / ID: 61 / Number of copies: 1619 / Formula: HOH
Molecular weight	Theoretical: 18.015 Da
Chemical component information	ChemComp-HOH: WATER / Water

-
Experimental details

-
Structure determination

Method	cryo EM
Processing	single particle reconstruction
Aggregation state	particle

-
Sample preparation

Buffer

pH: 7.5
Component:

Concentration	Formula	Name
20.0 mM	C₈H₁₈N₂O₄S	HEPES-KOH
95.0 mM	KCl	Potassium chloride
5.0 mM	NH₄Cl	Ammonium chloride
5.0 mM	Mg(OAc)₂	Magnesium acetate
0.5 mM	CaCl₂	Calcium chloride
8.0 mM	(CH₂)₄(NH₂)₂	Putrescine
1.0 mM	C₇H₁₉N₃	Spermidine
0.4 mM	C₃₃H₅₀O₆Na	Fusidic acid cyclopentane

Grid

Model: Quantifoil R2/1 / Material: COPPER / Mesh: 200 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 2 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 15 sec. / Pretreatment - Atmosphere: AIR / Pretreatment - Pressure: 0.039 kPa

Vitrification

Cryogen name: ETHANE / Chamber humidity: 90 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

Microscope	TFS KRIOS
Electron beam	Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron optics	C2 aperture diameter: 50.0 µm / Calibrated defocus max: 2.4 µm / Calibrated defocus min: 0.2 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 1.3 µm / Nominal defocus min: 0.7000000000000001 µm / Nominal magnification: 165000
Specialist optics	Energy filter - Name: TFS Selectris / Energy filter - Slit width: 10 eV
Sample stage	Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recording	Film or detector model: TFS FALCON 4i (4k x 4k) / Number grids imaged: 1 / Number real images: 17252 / Average exposure time: 2.23 sec. / Average electron dose: 27.77 e/Å²
Experimental equipment	Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Particle selection	Number selected: 559995
Startup model	Type of model: NONE
Initial angle assignment	Type: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 4.0.2)
Final 3D classification	Number classes: 5 / Avg.num./class: 10000 / Software - Name: cryoSPARC (ver. 4.1.2)
Final angle assignment	Type: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 4.1.2)
Final reconstruction	Number classes used: 4 / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 2.44 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4.1.2) / Number images used: 40800
FSC plot (resolution estimation)

-
Atomic model buiding 1

Initial model

PDB ID	Chain
7nhm	source_name: PDB, initial_model_type: experimental model
2xex	chain_id: E, source_name: PDB, initial_model_type: experimental model
7ssd	chain_id: D, source_name: PDB, initial_model_type: experimental model
7k00	chain_id: F, source_name: PDB, initial_model_type: experimental model

Refinement

Space: REAL / Protocol: FLEXIBLE FIT

Output model

PDB-8p2f:
Staphylococcus aureus 70S ribosome with elongation factor G locked with fusidic acid cyclopentane in post-translocational state

+
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Macromolecule	Name: ZINC ION / type: ligand / ID: 54 / Number of copies: 3 / Formula: ZN
Molecular weight	Theoretical: 65.409 Da

Macromolecule	Name: MAGNESIUM ION / type: ligand / ID: 55 / Number of copies: 145 / Formula: MG
Molecular weight	Theoretical: 24.305 Da

Macromolecule	Name: POTASSIUM ION / type: ligand / ID: 60 / Number of copies: 1 / Formula: K
Molecular weight	Theoretical: 39.098 Da

-Movie

-Structure viewers

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-Supplemental data

-Mask #1

-Additional map: Local map around EF-G

-Additional map: Unsharpened map

-Half map: Half map A

-Half map: Half map B

-Sample components

+Entire : Staphylococcus aureus 70S ribosome with elongation factor G and f...

+Supramolecule #1: Staphylococcus aureus 70S ribosome with elongation factor G and f...

+Macromolecule #1: 50S ribosomal protein L28

+Macromolecule #2: 50S ribosomal protein L29

+Macromolecule #3: 50S ribosomal protein L30

+Macromolecule #4: 50S ribosomal protein L31 type B

+Macromolecule #5: 50S ribosomal protein L32

+Macromolecule #6: 50S ribosomal protein L33 1

+Macromolecule #7: 50S ribosomal protein L34

+Macromolecule #8: 50S ribosomal protein L35

+Macromolecule #9: 50S ribosomal protein L36

+Macromolecule #13: Elongation factor G

+Macromolecule #14: 50S ribosomal protein L2

+Macromolecule #15: 50S ribosomal protein L3

+Macromolecule #16: 50S ribosomal protein L4

+Macromolecule #17: 50S ribosomal protein L5

+Macromolecule #18: 50S ribosomal protein L6

+Macromolecule #19: 50S ribosomal protein L13

+Macromolecule #20: 50S ribosomal protein L14

+Macromolecule #21: 50S ribosomal protein L15

+Macromolecule #22: 50S ribosomal protein L16

+Macromolecule #23: 50S ribosomal protein L17

+Macromolecule #24: 50S ribosomal protein L18

+Macromolecule #25: 50S ribosomal protein L19

+Macromolecule #26: 50S ribosomal protein L20

+Macromolecule #27: 50S ribosomal protein L21

+Macromolecule #28: 50S ribosomal protein L22

+Macromolecule #29: 50S ribosomal protein L23

+Macromolecule #30: 50S ribosomal protein L24

+Macromolecule #31: 50S ribosomal protein L25

+Macromolecule #32: 50S ribosomal protein L27

+Macromolecule #35: 30S ribosomal protein S2

+Macromolecule #36: 30S ribosomal protein S3

+Macromolecule #37: 30S ribosomal protein S4

+Macromolecule #38: 30S ribosomal protein S5

+Macromolecule #39: 30S ribosomal protein S6

+Macromolecule #40: 30S ribosomal protein S7

+Macromolecule #41: 30S ribosomal protein S8

+Macromolecule #42: 30S ribosomal protein S9

+Macromolecule #43: 30S ribosomal protein S10

+Macromolecule #44: 30S ribosomal protein S11

+Macromolecule #45: 30S ribosomal protein S12

+Macromolecule #46: 30S ribosomal protein S13

+Macromolecule #47: 30S ribosomal protein S14 type Z

+Macromolecule #48: 30S ribosomal protein S15

+Macromolecule #49: 30S ribosomal protein S16

+Macromolecule #50: 30S ribosomal protein S17

+Macromolecule #51: 30S ribosomal protein S18

+Macromolecule #52: 30S ribosomal protein S19

+Macromolecule #53: 30S ribosomal protein S20

+Macromolecule #10: 23S ribosomal RNA

+Macromolecule #11: 5S ribosomal RNA

+Macromolecule #12: tRNA

+Macromolecule #33: 16S ribosomal RNA

+Macromolecule #34: mRNA

+Macromolecule #54: ZINC ION

+Macromolecule #55: MAGNESIUM ION

+Macromolecule #56: SPERMIDINE

-
Movie

-
Structure viewers

+
Viewer log

-
This page

-
This web site

-
Options

+
Open data

-
Basic information

-
Structure visualization

-
Downloads & links

-
EMDB archive

-
Related structure data

-
Links

-
Map

Image control

-
Supplemental data

-
Mask #1

-
Additional map: Local map around EF-G

-
Additional map: Unsharpened map

-
Half map: Half map A

-
Half map: Half map B

-
Sample components

+
Entire : Staphylococcus aureus 70S ribosome with elongation factor G and f...

+
Supramolecule #1: Staphylococcus aureus 70S ribosome with elongation factor G and f...

+
Macromolecule #1: 50S ribosomal protein L28

+
Macromolecule #2: 50S ribosomal protein L29

+
Macromolecule #3: 50S ribosomal protein L30

+
Macromolecule #4: 50S ribosomal protein L31 type B

+
Macromolecule #5: 50S ribosomal protein L32

+
Macromolecule #6: 50S ribosomal protein L33 1

+
Macromolecule #7: 50S ribosomal protein L34

+
Macromolecule #8: 50S ribosomal protein L35

+
Macromolecule #9: 50S ribosomal protein L36

+
Macromolecule #13: Elongation factor G

+
Macromolecule #14: 50S ribosomal protein L2

+
Macromolecule #15: 50S ribosomal protein L3

+
Macromolecule #16: 50S ribosomal protein L4

+
Macromolecule #17: 50S ribosomal protein L5

+
Macromolecule #18: 50S ribosomal protein L6

+
Macromolecule #19: 50S ribosomal protein L13

+
Macromolecule #20: 50S ribosomal protein L14

+
Macromolecule #21: 50S ribosomal protein L15

+
Macromolecule #22: 50S ribosomal protein L16

+
Macromolecule #23: 50S ribosomal protein L17

+
Macromolecule #24: 50S ribosomal protein L18

+
Macromolecule #25: 50S ribosomal protein L19

+
Macromolecule #26: 50S ribosomal protein L20

+
Macromolecule #27: 50S ribosomal protein L21

+
Macromolecule #28: 50S ribosomal protein L22

+
Macromolecule #29: 50S ribosomal protein L23

+
Macromolecule #30: 50S ribosomal protein L24

+
Macromolecule #31: 50S ribosomal protein L25

+
Macromolecule #32: 50S ribosomal protein L27

+
Macromolecule #35: 30S ribosomal protein S2

+
Macromolecule #36: 30S ribosomal protein S3

+
Macromolecule #37: 30S ribosomal protein S4

+
Macromolecule #38: 30S ribosomal protein S5

+
Macromolecule #39: 30S ribosomal protein S6

+
Macromolecule #40: 30S ribosomal protein S7

+
Macromolecule #41: 30S ribosomal protein S8

+
Macromolecule #42: 30S ribosomal protein S9

+
Macromolecule #43: 30S ribosomal protein S10

+
Macromolecule #44: 30S ribosomal protein S11

+
Macromolecule #45: 30S ribosomal protein S12

+
Macromolecule #46: 30S ribosomal protein S13

+
Macromolecule #47: 30S ribosomal protein S14 type Z

+
Macromolecule #48: 30S ribosomal protein S15

+
Macromolecule #49: 30S ribosomal protein S16

+
Macromolecule #50: 30S ribosomal protein S17

+
Macromolecule #51: 30S ribosomal protein S18

+
Macromolecule #52: 30S ribosomal protein S19

+
Macromolecule #53: 30S ribosomal protein S20

+
Macromolecule #10: 23S ribosomal RNA

+
Macromolecule #11: 5S ribosomal RNA

+
Macromolecule #12: tRNA

+
Macromolecule #33: 16S ribosomal RNA

+
Macromolecule #34: mRNA

+
Macromolecule #54: ZINC ION

+
Macromolecule #55: MAGNESIUM ION

+
Macromolecule #56: SPERMIDINE