- EMDB-17268: ATM(Q2971A) dimeric C-terminal region in complex with Mg AMP-PNP -
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基本情報
登録情報
データベース: EMDB / ID: EMD-17268
タイトル
ATM(Q2971A) dimeric C-terminal region in complex with Mg AMP-PNP
マップデータ
Unsharpened map
試料
複合体: ATM(Q2971A) dimeric C-terminal region bound to Mg AMP-PNP
タンパク質・ペプチド: Serine-protein kinase ATM
リガンド: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER
リガンド: MAGNESIUM ION
リガンド: ZINC ION
キーワード
Ataxia-Telangiectasia Mutated / ATM / kinase / SIGNALING PROTEIN
機能・相同性
機能・相同性情報
positive regulation of DNA catabolic process / establishment of RNA localization to telomere / positive regulation of telomerase catalytic core complex assembly / positive regulation of DNA damage response, signal transduction by p53 class mediator / cellular response to nitrosative stress / establishment of protein-containing complex localization to telomere / negative regulation of telomere capping / Sensing of DNA Double Strand Breaks / positive regulation of telomere maintenance via telomere lengthening / regulation of microglial cell activation ...positive regulation of DNA catabolic process / establishment of RNA localization to telomere / positive regulation of telomerase catalytic core complex assembly / positive regulation of DNA damage response, signal transduction by p53 class mediator / cellular response to nitrosative stress / establishment of protein-containing complex localization to telomere / negative regulation of telomere capping / Sensing of DNA Double Strand Breaks / positive regulation of telomere maintenance via telomere lengthening / regulation of microglial cell activation / meiotic telomere clustering / pre-B cell allelic exclusion / DNA-dependent protein kinase activity / histone H2AXS139 kinase activity / male meiotic nuclear division / histone mRNA catabolic process / female meiotic nuclear division / pexophagy / cellular response to X-ray / regulation of telomere maintenance via telomerase / peptidyl-serine autophosphorylation / DNA double-strand break processing / lipoprotein catabolic process / V(D)J recombination / regulation of autophagosome assembly / oocyte development / Impaired BRCA2 binding to PALB2 / reciprocal meiotic recombination / DNA repair complex / Defective homologous recombination repair (HRR) due to BRCA1 loss of function / Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA1 binding function / Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA2/RAD51/RAD51C binding function / Homologous DNA Pairing and Strand Exchange / Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA) / Resolution of D-loop Structures through Holliday Junction Intermediates / HDR through Single Strand Annealing (SSA) / Impaired BRCA2 binding to RAD51 / 1-phosphatidylinositol-3-kinase activity / response to ionizing radiation / mitotic spindle assembly checkpoint signaling / TP53 Regulates Transcription of Caspase Activators and Caspases / negative regulation of B cell proliferation / mitotic G2 DNA damage checkpoint signaling / Presynaptic phase of homologous DNA pairing and strand exchange / TP53 Regulates Transcription of Genes Involved in Cytochrome C Release / peroxisomal matrix / positive regulation of cell adhesion / replicative senescence / Regulation of HSF1-mediated heat shock response / signal transduction in response to DNA damage / somitogenesis / regulation of cellular response to heat / cellular response to retinoic acid / ovarian follicle development / negative regulation of TORC1 signaling / positive regulation of telomere maintenance via telomerase / DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest / Pexophagy / telomere maintenance / post-embryonic development / thymus development / regulation of signal transduction by p53 class mediator / DNA damage checkpoint signaling / regulation of autophagy / determination of adult lifespan / TP53 Regulates Transcription of DNA Repair Genes / Nonhomologous End-Joining (NHEJ) / Stabilization of p53 / Autodegradation of the E3 ubiquitin ligase COP1 / double-strand break repair via homologous recombination / brain development / multicellular organism growth / G2/M DNA damage checkpoint / HDR through Homologous Recombination (HRR) / Regulation of TP53 Activity through Methylation / DNA Damage/Telomere Stress Induced Senescence / cellular response to gamma radiation / Meiotic recombination / cellular response to reactive oxygen species / spindle / double-strand break repair via nonhomologous end joining / intrinsic apoptotic signaling pathway in response to DNA damage / positive regulation of neuron apoptotic process / cellular senescence / double-strand break repair / Regulation of TP53 Degradation / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / heart development / site of double-strand break / Processing of DNA double-strand break ends / cytoplasmic vesicle / peptidyl-serine phosphorylation / neuron apoptotic process / regulation of apoptotic process / Regulation of TP53 Activity through Phosphorylation / protein autophosphorylation / response to hypoxia / regulation of cell cycle / non-specific serine/threonine protein kinase / positive regulation of cell migration 類似検索 - 分子機能
Telomere-length maintenance and DNA damage repair / Serine/threonine-protein kinase ATM, plant / ATM, catalytic domain / Telomere-length maintenance and DNA damage repair / Telomere-length maintenance and DNA damage repair / FATC domain / PIK-related kinase, FAT / FAT domain / FATC / FATC domain ...Telomere-length maintenance and DNA damage repair / Serine/threonine-protein kinase ATM, plant / ATM, catalytic domain / Telomere-length maintenance and DNA damage repair / Telomere-length maintenance and DNA damage repair / FATC domain / PIK-related kinase, FAT / FAT domain / FATC / FATC domain / PIK-related kinase / FAT domain profile. / FATC domain profile. / Phosphatidylinositol 3- and 4-kinases signature 1. / Phosphatidylinositol 3/4-kinase, conserved site / Phosphatidylinositol 3- and 4-kinases signature 2. / Phosphatidylinositol 3-/4-kinase, catalytic domain superfamily / Phosphoinositide 3-kinase, catalytic domain / Phosphatidylinositol 3- and 4-kinase / Phosphatidylinositol 3- and 4-kinases catalytic domain profile. / Phosphatidylinositol 3-/4-kinase, catalytic domain / Armadillo-type fold / Protein kinase-like domain superfamily 類似検索 - ドメイン・相同性
ジャーナル: Sci Adv / 年: 2023 タイトル: Structural insights into the activation of ataxia-telangiectasia mutated by oxidative stress. 著者: Anna C Howes / Olga Perisic / Roger L Williams / 要旨: Ataxia-telangiectasia mutated (ATM) is a master kinase regulating DNA damage response that is activated by DNA double-strand breaks. However, ATM is also directly activated by reactive oxygen ...Ataxia-telangiectasia mutated (ATM) is a master kinase regulating DNA damage response that is activated by DNA double-strand breaks. However, ATM is also directly activated by reactive oxygen species, but how oxidative activation is achieved remains unknown. We determined the cryo-EM structure of an HO-activated ATM and showed that under oxidizing conditions, ATM formed an intramolecular disulfide bridge between two protomers that are rotated relative to each other when compared to the basal state. This rotation is accompanied by release of the substrate-blocking PRD region and twisting of the N-lobe relative to the C-lobe, which greatly optimizes catalysis. This active site remodeling enabled us to capture a substrate (p53) bound to the enzyme. This provides the first structural insights into how ATM is activated during oxidative stress.
全体 : ATM(Q2971A) dimeric C-terminal region bound to Mg AMP-PNP
全体
名称: ATM(Q2971A) dimeric C-terminal region bound to Mg AMP-PNP
要素
複合体: ATM(Q2971A) dimeric C-terminal region bound to Mg AMP-PNP
タンパク質・ペプチド: Serine-protein kinase ATM
リガンド: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER
リガンド: MAGNESIUM ION
リガンド: ZINC ION
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超分子 #1: ATM(Q2971A) dimeric C-terminal region bound to Mg AMP-PNP
超分子
名称: ATM(Q2971A) dimeric C-terminal region bound to Mg AMP-PNP タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1 詳細: The map deposited is from local refinement on the C-terminal region of the ATM dimer to obtain higher resolution details. Please note the sample contains the whole ATM dimer itself and not ...詳細: The map deposited is from local refinement on the C-terminal region of the ATM dimer to obtain higher resolution details. Please note the sample contains the whole ATM dimer itself and not just the C-terminal region.