TPC2 / two-pore channel / ion channel / inhibitor / homodimer / METAL TRANSPORT
Function / homology
Function and homology information
endosome to lysosome transport of low-density lipoprotein particle / negative regulation of developmental pigmentation / intracellular pH reduction / intracellularly phosphatidylinositol-3,5-bisphosphate-gated monatomic cation channel activity / ligand-gated sodium channel activity / NAADP-sensitive calcium-release channel activity / melanosome membrane / regulation of exocytosis / response to vitamin D / endolysosome membrane ...endosome to lysosome transport of low-density lipoprotein particle / negative regulation of developmental pigmentation / intracellular pH reduction / intracellularly phosphatidylinositol-3,5-bisphosphate-gated monatomic cation channel activity / ligand-gated sodium channel activity / NAADP-sensitive calcium-release channel activity / melanosome membrane / regulation of exocytosis / response to vitamin D / endolysosome membrane / phosphatidylinositol-3,5-bisphosphate binding / monoatomic ion channel complex / lysosome organization / sodium ion transmembrane transport / smooth muscle contraction / voltage-gated calcium channel activity / release of sequestered calcium ion into cytosol / monoatomic ion transmembrane transport / regulation of autophagy / calcium-mediated signaling / calcium channel activity / endocytosis involved in viral entry into host cell / Stimuli-sensing channels / intracellular calcium ion homeostasis / late endosome membrane / receptor-mediated endocytosis of virus by host cell / lysosome / endosome membrane / lysosomal membrane / protein kinase binding / identical protein binding / cytosol Similarity search - Function
Two pore channel protein 2 / Voltage-dependent channel domain superfamily / Ion transport domain / Ion transport protein Similarity search - Domain/homology
Journal: Structure / Year: 2024 Title: Structural basis for inhibition of the lysosomal two-pore channel TPC2 by a small molecule antagonist. Authors: Gamma Chi / Dawid Jaślan / Veronika Kudrina / Julia Böck / Huanyu Li / Ashley C W Pike / Susanne Rautenberg / Einar Krogsaeter / Tina Bohstedt / Dong Wang / Gavin McKinley / Alejandra ...Authors: Gamma Chi / Dawid Jaślan / Veronika Kudrina / Julia Böck / Huanyu Li / Ashley C W Pike / Susanne Rautenberg / Einar Krogsaeter / Tina Bohstedt / Dong Wang / Gavin McKinley / Alejandra Fernandez-Cid / Shubhashish M M Mukhopadhyay / Nicola A Burgess-Brown / Marco Keller / Franz Bracher / Christian Grimm / Katharina L Dürr / Abstract: Two pore channels are lysosomal cation channels with crucial roles in tumor angiogenesis and viral release from endosomes. Inhibition of the two-pore channel 2 (TPC2) has emerged as potential ...Two pore channels are lysosomal cation channels with crucial roles in tumor angiogenesis and viral release from endosomes. Inhibition of the two-pore channel 2 (TPC2) has emerged as potential therapeutic strategy for the treatment of cancers and viral infections, including Ebola and COVID-19. Here, we demonstrate that antagonist SG-094, a synthetic analog of the Chinese alkaloid medicine tetrandrine with increased potency and reduced toxicity, induces asymmetrical structural changes leading to a single binding pocket at only one intersubunit interface within the asymmetrical dimer. Supported by functional characterization of mutants by Ca imaging and patch clamp experiments, we identify key residues in S1 and S4 involved in compound binding to the voltage sensing domain II. SG-094 arrests IIS4 in a downward shifted state which prevents pore opening via the IIS4/S5 linker, hence resembling gating modifiers of canonical VGICs. These findings may guide the rational development of new therapeutics antagonizing TPC2 activity.
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