- EMDB-17187: 48-nm repeat of the native axonemal doublet microtubule from bovi... -
+
データを開く
IDまたはキーワード:
読み込み中...
-
基本情報
登録情報
データベース: EMDB / ID: EMD-17187
タイトル
48-nm repeat of the native axonemal doublet microtubule from bovine sperm
マップデータ
試料
複合体: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm
タンパク質・ペプチド: x 66種
リガンド: x 3種
キーワード
microtubule / cilia / sperm / axoneme / STRUCTURAL PROTEIN
機能・相同性
機能・相同性情報
axonemal microtubule doublet inner sheath / outer acrosomal membrane / epithelial cilium movement involved in determination of left/right asymmetry / regulation of brood size / establishment of left/right asymmetry / 9+0 motile cilium / sperm flagellum assembly / manchette assembly / axonemal B tubule inner sheath / axonemal A tubule inner sheath ...axonemal microtubule doublet inner sheath / outer acrosomal membrane / epithelial cilium movement involved in determination of left/right asymmetry / regulation of brood size / establishment of left/right asymmetry / 9+0 motile cilium / sperm flagellum assembly / manchette assembly / axonemal B tubule inner sheath / axonemal A tubule inner sheath / manchette / sperm axoneme assembly / protein polyglutamylation / inner dynein arm assembly / regulation of calcineurin-NFAT signaling cascade / positive regulation of feeding behavior / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Cilium Assembly / Intraflagellar transport / Carboxyterminal post-translational modifications of tubulin / Sealing of the nuclear envelope (NE) by ESCRT-III / Kinesins / Resolution of Sister Chromatid Cohesion / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / COPI-dependent Golgi-to-ER retrograde traffic / cilium-dependent cell motility / COPI-independent Golgi-to-ER retrograde traffic / COPI-mediated anterograde transport / MAP kinase tyrosine/serine/threonine phosphatase activity / RHO GTPases activate IQGAPs / cerebrospinal fluid circulation / sperm principal piece / regulation of cilium beat frequency involved in ciliary motility / RHO GTPases Activate Formins / cilium movement involved in cell motility / regulation of store-operated calcium entry / 転移酵素; リンを含む基を移すもの / MHC class II antigen presentation / acrosomal membrane / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / ciliary transition zone / cilium movement / axoneme assembly / Aggrephagy / sperm DNA condensation / calcium ion sensor activity / axonemal microtubule / The role of GTSE1 in G2/M progression after G2 checkpoint / Separation of Sister Chromatids / cilium organization / Loss of Nlp from mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Loss of proteins required for interphase microtubule organization from the centrosome / Anchoring of the basal body to the plasma membrane / AURKA Activation by TPX2 / Recruitment of NuMA to mitotic centrosomes / gamma-tubulin ring complex / flagellated sperm motility / Regulation of PLK1 Activity at G2/M Transition / Hedgehog 'off' state / UTP biosynthetic process / CTP biosynthetic process / determination of left/right symmetry / Neutrophil degranulation / motile cilium / nucleoside diphosphate kinase activity / GTP biosynthetic process / positive regulation of cell motility / histone H2AXS139 phosphatase activity / RNA polymerase II CTD heptapeptide repeat Y1 phosphatase activity / RNA polymerase II CTD heptapeptide repeat T4 phosphatase activity / RNA polymerase II CTD heptapeptide repeat S2 phosphatase activity / RNA polymerase II CTD heptapeptide repeat S5 phosphatase activity / RNA polymerase II CTD heptapeptide repeat S7 phosphatase activity / MAP kinase serine/threonine phosphatase activity / calmodulin-dependent protein phosphatase activity / myosin phosphatase activity / AMP binding / regulation of neuron projection development / ciliary base / beta-tubulin binding / spermatid development / cerebral cortex cell migration / microtubule organizing center / regulation of cell division / mitotic cytokinesis / sperm flagellum / cellular response to UV-C / axoneme / cilium assembly / glial cell projection / single fertilization / alpha-tubulin binding / phosphatase binding / intercellular bridge / cytoplasmic microtubule / 3'-5' exonuclease activity / sperm midpiece / centriole 類似検索 - 分子機能
Protein of unknown function DUF4542 / Uncharacterized protein C1orf100 / Sperm microtubule associated protein 1 / Family of unknown function (DUF5530) / Testicular haploid expressed repeat / Uncharacterised protein family C20orf85 / Protein of unknown function DUF4562 / Spermatid-specific manchette-related protein 1 / Testis-expressed protein 33 / Testis-expressed sequence 37 protein ...Protein of unknown function DUF4542 / Uncharacterized protein C1orf100 / Sperm microtubule associated protein 1 / Family of unknown function (DUF5530) / Testicular haploid expressed repeat / Uncharacterised protein family C20orf85 / Protein of unknown function DUF4562 / Spermatid-specific manchette-related protein 1 / Testis-expressed protein 33 / Testis-expressed sequence 37 protein / CFAP97 domain-containing protein 1/2 / Testicular haploid expressed gene protein-like / Testis-specific serine/threonine-protein kinase 6, catalytic domain / C10orf82-like / : / : / : / Testicular haploid expressed repeat / Normal lung function maintenance, Low in Lung Cancer 1 protein / Domain of unknown function (DUF4562) / Spermatid-specific manchette-related protein 1 / TSC21 family / Testis-expressed sequence 33 protein family / Sperm-associated microtubule inner protein 5 / Ciliary microtubule inner protein 3 / Sperm microtubule inner protein 1 / Repeats in THEG (testicular haploid expressed gene) and several fly proteins. / Protein phosphatase 1 regulatory subunit 32 / Testis, prostate and placenta-expressed protein / Testis-expressed protein 49 / Protein phosphatase 1 regulatory subunit 32 / Sperm microtubule inner protein 8 / Sperm microtubule inner protein 11 / : / Protein of unknown function DUF1143 / Atypical dual specificity phosphatase, subfamily B / Protein of unknown function DUF4513 / Hemingway/CFA97 / Cilia- and flagella-associated protein 68 / Hemingway/CFA97 / Sperm-associated microtubule inner protein 10 / Protein of unknown function DUF4541 / Tektin bundle interacting protein 1 / Coiled-coil domain-containing protein 105 / Tektin bundle interacting protein 1 / Cilia and flagella associated protein 90 / DJBP, EF-hand domain / Protein of unknown function DUF4586 / : / EF-hand domain / Cilia-and flagella-associated protein 96 / Sperm-tail PG-rich repeat / FAM183 family / Sperm-tail PG-rich repeat / FAM183A and FAM183B related / Sperm-associated antigen 8 / Cilia- and flagella-associated protein 95 / NDPK7, first NDPk domain / Cilia- and flagella-associated protein 107 / : / Protein CFAP95 / Cilia- and flagella-associated protein 143 / Cilia and flagella associated protein 107 / Tektin / Cilia- and flagella-associated protein 276 / Sperm acrosome-associated protein 9 / : / Tektin family / Protein of unknown function (DUF3695) / Sperm acrosome-associated protein 9 / Uncharacterised protein FAM166/UPF0605 / Cilia- and flagella-associated protein 77 / Ciliary microtubule inner protein 2B-like / Cilia- and flagella-associated protein 77 / Nucleoside diphosphate kinase 7 / Piercer of microtubule wall 1/2 / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 141 / Cilia- and flagella-associated protein 141 / Cilia- and flagella-associated protein 161-like domain / : / : / Meiosis-specific nuclear structural protein 1 / Cilia- and flagella-associated protein 45 / NDPK7, second NDPk domain / Cilia- and flagella- associated protein 210 / CFAP53/TCHP / Trichohyalin-plectin-homology domain / Trichohyalin-plectin-homology domain / RIB43A / RIB43A / DM10 domain / EF-hand domain-containing protein EFHC1/EFHC2/EFHB / DM10 domain / DM10 domain profile. / Domains in hypothetical proteins in Drosophila, C. elegans and mammals. Occurs singly in some nucleoside diphosphate kinases. / Enkurin domain / : / Calmodulin-binding / Enkurin domain profile. 類似検索 - ドメイン・相同性
Ciliary microtubule inner protein 3 / Piercer of microtubule wall 2 protein / Cilia and flagella associated protein 77 / Cilia and flagella associated protein 90 / Sperm microtubule associated protein 2 like / Sperm microtubule inner protein 11 / Sperm acrosome associated 9 / EF-hand domain-containing family member C2 / Ciliary microtubule inner protein 1 / Protein SPMIP9 ...Ciliary microtubule inner protein 3 / Piercer of microtubule wall 2 protein / Cilia and flagella associated protein 77 / Cilia and flagella associated protein 90 / Sperm microtubule associated protein 2 like / Sperm microtubule inner protein 11 / Sperm acrosome associated 9 / EF-hand domain-containing family member C2 / Ciliary microtubule inner protein 1 / Protein SPMIP9 / Parkin coregulated gene protein / Tektin-3 / Sperm-associated microtubule inner protein 5 / Cilia- and flagella-associated protein 144 / Sperm-associated microtubule inner protein 10 / Sperm microtubule inner protein 8 / Enkurin / Cilia- and flagella-associated protein 276 / Sperm microtubule associated protein 1 / Cilia- and flagella- associated protein 210 / Cilia- and flagella-associated protein 52 / EF-hand domain-containing protein 1 / Sperm-associated antigen 8 / EF-hand domain-containing family member B / Tektin bundle interacting protein 1 / non-specific serine/threonine protein kinase / Cilia- and flagella-associated protein 53 / Cilia- and flagella-associated protein 161 / CFAP97 domain containing 1 / Outer dense fiber of sperm tails 3 / Ciliary microtubule inner protein 2A / Sperm microtubule inner protein 1 / EF-hand calcium binding domain 6 / Dual specificity phosphatase 21 / Meiosis-specific nuclear structural protein 1 / Protein SPMIP2 / Cilia-and flagella-associated protein 96 / EF-hand calcium-binding domain-containing protein 3 / Cilia- and flagella-associated protein 68 / Tektin-2 / Stabilizer of axonemal microtubules 4 / Cilia- and flagella-associated protein 107 / Tektin-4 / Tektin-5 / Tektin-like protein 1 / Tubulin alpha-3 chain / Sperm microtubule inner protein 6 / Tektin-1 / Protein SPMIP3 / Cilia- and flagella-associated protein 141 / Cilia- and flagella-associated protein 95 / Ciliary microtubule inner protein 4 / RIB43A-like with coiled-coils protein 2 / Cilia- and flagella-associated protein 45 / Piercer of microtubule wall 1 protein / Tubulin beta-4B chain / Ciliary microtubule inner protein 2C / Nucleoside diphosphate kinase homolog 7 / Cilia- and flagella-associated protein 20 類似検索 - 構成要素
Netherlands Organisation for Scientific Research (NWO)
740.018.007
オランダ
引用
ジャーナル: Cell / 年: 2023 タイトル: Structural specializations of the sperm tail. 著者: Miguel Ricardo Leung / Jianwei Zeng / Xiangli Wang / Marc C Roelofs / Wei Huang / Riccardo Zenezini Chiozzi / Johannes F Hevler / Albert J R Heck / Susan K Dutcher / Alan Brown / Rui Zhang / ...著者: Miguel Ricardo Leung / Jianwei Zeng / Xiangli Wang / Marc C Roelofs / Wei Huang / Riccardo Zenezini Chiozzi / Johannes F Hevler / Albert J R Heck / Susan K Dutcher / Alan Brown / Rui Zhang / Tzviya Zeev-Ben-Mordehai / 要旨: Sperm motility is crucial to reproductive success in sexually reproducing organisms. Impaired sperm movement causes male infertility, which is increasing globally. Sperm are powered by a microtubule- ...Sperm motility is crucial to reproductive success in sexually reproducing organisms. Impaired sperm movement causes male infertility, which is increasing globally. Sperm are powered by a microtubule-based molecular machine-the axoneme-but it is unclear how axonemal microtubules are ornamented to support motility in diverse fertilization environments. Here, we present high-resolution structures of native axonemal doublet microtubules (DMTs) from sea urchin and bovine sperm, representing external and internal fertilizers. We identify >60 proteins decorating sperm DMTs; at least 15 are sperm associated and 16 are linked to infertility. By comparing DMTs across species and cell types, we define core microtubule inner proteins (MIPs) and analyze evolution of the tektin bundle. We identify conserved axonemal microtubule-associated proteins (MAPs) with unique tubulin-binding modes. Additionally, we identify a testis-specific serine/threonine kinase that links DMTs to outer dense fibers in mammalian sperm. Our study provides structural foundations for understanding sperm evolution, motility, and dysfunction at a molecular level.