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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-1711 | ||||||||||||
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Title | Pyrococcus furiosus RNA Polymerase | ||||||||||||
![]() | Pyrococus furiosus RNA Polymerase, threshold masked map, gaussian filtered to 13 Angstrom | ||||||||||||
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![]() | Archaea / RNA Polymerase | ||||||||||||
Biological species | ![]() ![]() | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 13.0 Å | ||||||||||||
![]() | Kusser AG / Bertero MG / Naji S / Becker T / Thomm M / Beckmann R / Cramer P | ||||||||||||
![]() | ![]() Title: Structure of an archaeal RNA polymerase. Authors: Anselm G Kusser / Michela G Bertero / Souad Naji / Thomas Becker / Michael Thomm / Roland Beckmann / Patrick Cramer / ![]() Abstract: Related multisubunit RNA polymerases (RNAPs) carry out gene transcription in all kingdoms of life. Since structural information is limited to bacterial and eukaryotic RNAPs, we determined the cryo- ...Related multisubunit RNA polymerases (RNAPs) carry out gene transcription in all kingdoms of life. Since structural information is limited to bacterial and eukaryotic RNAPs, we determined the cryo-electron microscopic structure of the RNAP from the thermophilic archaeon Pyrococcus furiosus at 13 A resolution. Comparison with eukaryotic RNAP II reveals a conserved architecture, no homologues for subunits Rpb8 and Rpb9, and significant deviation in the polymerase foot, jaws, pore, and protrusion. The structural organization of the archaeal RNA polymerase serves as a reference for future structure-function analysis of the transcription mechanism and allows for evolutionary comparisons. | ||||||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 21.2 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 9.5 KB 9.5 KB | Display Display | ![]() |
Images | ![]() | 86.6 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 196.8 KB | Display | ![]() |
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Full document | ![]() | 196 KB | Display | |
Data in XML | ![]() | 5.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Pyrococus furiosus RNA Polymerase, threshold masked map, gaussian filtered to 13 Angstrom | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.2375 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : Endogeneous RNA Polymerase from Pyrococcus furiosus
Entire | Name: Endogeneous RNA Polymerase from Pyrococcus furiosus |
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Components |
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-Supramolecule #1000: Endogeneous RNA Polymerase from Pyrococcus furiosus
Supramolecule | Name: Endogeneous RNA Polymerase from Pyrococcus furiosus / type: sample / ID: 1000 / Details: The sample was monodisperse / Oligomeric state: Monomeric / Number unique components: 11 |
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Molecular weight | Experimental: 380 KDa / Theoretical: 380 KDa |
-Macromolecule #1: DNA directed RNA polymerase
Macromolecule | Name: DNA directed RNA polymerase / type: protein_or_peptide / ID: 1 / Name.synonym: RNA polymerase / Oligomeric state: Monomer / Recombinant expression: No |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Experimental: 380 KDa / Theoretical: 380 KDa |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 0.1 mg/mL |
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Buffer | pH: 7 Details: 10 mM HEPES (pH 7.0), 150 mM KCl, 2.5 mM MgCl2, 5 mM DTT |
Grid | Details: Quantifoil R3/3 w. 2nm Carbon on top |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 277 K / Instrument: OTHER / Details: Vitrification instrument: Vitrobot / Method: Blot for 10 seconds |
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Electron microscopy
Microscope | FEI TECNAI F30 |
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Temperature | Average: 80 K |
Image recording | Category: FILM / Film or detector model: KODAK SO-163 FILM / Digitization - Scanner: OTHER / Digitization - Sampling interval: 7 µm / Number real images: 23 / Average electron dose: 25 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Calibrated magnification: 67000 / Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Cs: 2.0 mm / Nominal defocus max: 3.5 µm / Nominal defocus min: 1.5 µm / Nominal magnification: 67000 |
Sample stage | Specimen holder: Multi specimen / Specimen holder model: GATAN LIQUID NITROGEN |
Experimental equipment | ![]() Model: Tecnai F30 / Image courtesy: FEI Company |
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Image processing
CTF correction | Details: Wiener filtering of defocus group volumes |
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Final reconstruction | Applied symmetry - Point group: C1 (asymmetric) / Algorithm: OTHER / Resolution.type: BY AUTHOR / Resolution: 13.0 Å / Resolution method: FSC 0.5 CUT-OFF / Software - Name: SPIDER / Details: Final back projection in real space / Number images used: 22240 |
-Atomic model buiding 1
Initial model | PDB ID: |
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Details | Fitted using UCSF Chimera |
Refinement | Space: REAL |