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- EMDB-17040: Cryo-EM structure of P5A-ATPase CtSpf1 (E1-ATP state) -

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Basic information

Entry
Database: EMDB / ID: EMD-17040
TitleCryo-EM structure of P5A-ATPase CtSpf1 (E1-ATP state)
Map data
Sample
  • Complex: CtSpf1 with AMPPNP bound
    • Protein or peptide: Cation-transporting ATPase-like protein
  • Ligand: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER
  • Ligand: MAGNESIUM ION
Keywordstranslocase / MEMBRANE PROTEIN
Function / homology
Function and homology information


P-type ion transporter activity / ATPase-coupled monoatomic cation transmembrane transporter activity / intracellular calcium ion homeostasis / endoplasmic reticulum membrane / ATP hydrolysis activity / ATP binding
Similarity search - Function
: / : / P5A-ATPase, transmembrane helical hairpin / P-type ATPase, subfamily V / P-type ATPase actuator domain / P-type ATPase, haloacid dehalogenase domain / P-type ATPase, phosphorylation site / P-type ATPase, cytoplasmic domain N / E1-E2 ATPases phosphorylation site. / P-type ATPase, A domain superfamily ...: / : / P5A-ATPase, transmembrane helical hairpin / P-type ATPase, subfamily V / P-type ATPase actuator domain / P-type ATPase, haloacid dehalogenase domain / P-type ATPase, phosphorylation site / P-type ATPase, cytoplasmic domain N / E1-E2 ATPases phosphorylation site. / P-type ATPase, A domain superfamily / P-type ATPase / P-type ATPase, transmembrane domain superfamily / HAD superfamily / HAD-like superfamily
Similarity search - Domain/homology
Cation-transporting ATPase-like protein
Similarity search - Component
Biological speciesThermochaetoides thermophila (fungus)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.2 Å
AuthorsLi P / Gourdon P
Funding support Sweden, Denmark, 4 items
OrganizationGrant numberCountry
Knut and Alice Wallenberg Foundation2020.0194 Sweden
Swedish Research Council2020-01315 Sweden
The Crafoord Foundation20200739 Sweden
LundbeckfondenR346-2020-2019 Denmark
Citation
Journal: Nat Commun / Year: 2024
Title: The structure and function of P5A-ATPases.
Authors: Ping Li / Viktoria Bågenholm / Per Hägglund / Karin Lindkvist-Petersson / Kaituo Wang / Pontus Gourdon /
Abstract: Endoplasmic reticulum (ER) membrane resident P5A-ATPases broadly affect protein biogenesis and quality control, and yet their molecular function remains debated. Here, we report cryo-EM structures of ...Endoplasmic reticulum (ER) membrane resident P5A-ATPases broadly affect protein biogenesis and quality control, and yet their molecular function remains debated. Here, we report cryo-EM structures of a P5A-ATPase, CtSpf1, covering multiple transport intermediates of the E1 → E1-ATP → E1P-ADP → E1P → E2P → E2.P → E2 → E1 cycle. In the E2P and E2.P states a cleft spans the entire membrane, holding a polypeptide cargo molecule. The cargo includes an ER luminal extension, pinpointed as the C-terminus in the E2.P state, which reenters the membrane in E2P. The E1 structure harbors a cytosol-facing cavity that is blocked by an insertion we refer to as the Plug-domain. The Plug-domain is nestled to key ATPase features and is displaced in the E1P-ADP and E1P states. Collectively, our findings are compatible with a broad range of proteins as cargo, with the P5A-ATPases serving a role in membrane removal of helices, although insertion/secretion cannot be excluded, as well as with a mechanistic role of the Plug-domain.
#1: Journal: Acta Crystallogr., Sect. D: Biol. Crystallogr. / Year: 2018
Title: Real-space refinement in PHENIX for cryo-EM and crystallography
Authors: Li P / Gourdon P
History
DepositionApr 6, 2023-
Header (metadata) releaseOct 16, 2024-
Map releaseOct 16, 2024-
UpdateNov 20, 2024-
Current statusNov 20, 2024Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_17040.png

Downloads & links

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Map

FileDownload / File: emd_17040.map.gz / Format: CCP4 / Size: 163.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
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Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.83 Å/pix.
x 350 pix.
= 291.2 Å		0.83 Å/pix.
x 350 pix.
= 291.2 Å		0.83 Å/pix.
x 350 pix.
= 291.2 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.832 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.5
Minimum - Maximum-2.4275725 - 4.0764995
Average (Standard dev.)0.00045664655 (±0.078135885)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions350350350
Spacing350350350
CellA=B=C: 291.2 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_17040_half_map_1.map
Projections & Slices
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Slices (1/2)
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Half map: #1

Fileemd_17040_half_map_2.map
Projections & Slices
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Sample components

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Entire : CtSpf1 with AMPPNP bound

EntireName: CtSpf1 with AMPPNP bound
Components
  • Complex: CtSpf1 with AMPPNP bound
    • Protein or peptide: Cation-transporting ATPase-like protein
  • Ligand: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER
  • Ligand: MAGNESIUM ION

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Supramolecule #1: CtSpf1 with AMPPNP bound

SupramoleculeName: CtSpf1 with AMPPNP bound / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Thermochaetoides thermophila (fungus)

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Macromolecule #1: Cation-transporting ATPase-like protein

MacromoleculeName: Cation-transporting ATPase-like protein / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 149.103156 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MAPLVDNPQI KSAELLRPLP LYQHAYVWPY VIVWPVFLRV YLTQELYDKY IGAQEWTFVW IISIVTFQTL TWLCTHWSVN LNALFTAKK ASSIEDAQLI KVIPVANAGA ADICKLVRDK VGDNKTNISF LFQKRRFLWY PERKAFSTLE FDIDAEPKPT L SKFQLSRG ...String:
MAPLVDNPQI KSAELLRPLP LYQHAYVWPY VIVWPVFLRV YLTQELYDKY IGAQEWTFVW IISIVTFQTL TWLCTHWSVN LNALFTAKK ASSIEDAQLI KVIPVANAGA ADICKLVRDK VGDNKTNISF LFQKRRFLWY PERKAFSTLE FDIDAEPKPT L SKFQLSRG IESEDELKRL EQHYGTNTFD IPVPTFTELF KEHAVAPFFV FQVFCVGLWL LDEYWYYSLF TLVMLVVFES TV VWQRQRT LTEFRSMSIK PYPIYVYRLG KWTEIQSDKL LPGDLVSVTR TKEDSGVACD MILVEGTAIV NEAMLSGEST PLL KDSIQL RPGDAVLEVD GLDKNSLLWG GTKVLQITHG TAEEERPKPA SGIPPPPDNG AMAVVTKTGF ETSQGSLVRT MIYS TERVS ANNTEALLFI LFLLVFALAA SWYVWDEGVR KDRKRSKLLL DCILIITSVV PPELPMELSL AVNTSLSALA KFAIF CTEP FRIPFAGRID VACFDKTGTL TGEDLVVEGI AGLGLGHSGT DTPKEADGAH TRMVSVHDAG METTLVLATA HALVKL DEG EIVGDPMEKA TLNALGWVLG KNDTLTSKPG NAASSGILGT VQIKRRFQFS SALKRQSSVA TITATEVKTG RKLRGSF VG VKGAPETIMK MLVTVPEHYE ETYKYFTRRG SRVLALAYKQ LTTEGELGAN KINDLKRESV EADLHFAGFL VLQCPLKE D AKQAVRMLNE SSHRVVMITG DNPLTAVHVA KEVEIVDRDV LILDAPEHSV YGEESLVWRS VDDKIRIDVD PTKPIDPEI LKTKDLCVTG YALNKFKGQV GWKSLLRYTW VYARVSPKQK EDILLGLKDM GYYTLMAGDG TNDVGALKQA HVGVALLNGT QEDLNRIAE HTRNQKMKEL YQKQVDLMAR WGQPPPPVPA MIAHLYPPGP SNPHYQKAME REAQKRGVTV EQLAKVNGTN V TSNPAGVQ QQSGQDAKKA KQVEAAKKAA NFADKLTSSL MEAEMDDEPP TLKLGDASVA APFTSKLRNV MAIPNILRQG RC TLVATIQ MYKILALNCL ISAYSLSVLY LEGIKFGDGQ ITISGMLMSV CFLSISRARS VEGLSKERPQ PNIFNFYIIG SIL GQFAVH VATLIYIAQL CDQIEPRTEV IDLEAEFKPS LLNSAVYLLQ LIQQISTFAV NYQGRPFRES LSENKGMFYG IVGV TAIAF ACSTEMLPEL NEAMKLVPFN ENFKTIMTTV MIIDFVACYV IEWVLKKLFS DLRARDIAER RPDQLERERV RKEKE AREK EEEEERKERE RIEAFERRLE EKRTRLVEAA AQREQQQQQW AQRR

UniProtKB: Cation-transporting ATPase-like protein

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Macromolecule #2: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER

MacromoleculeName: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / type: ligand / ID: 2 / Number of copies: 1 / Formula: ANP
Molecular weightTheoretical: 506.196 Da
Chemical component information

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

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Macromolecule #3: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 3 / Number of copies: 1 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Average electron dose: 40.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.6 µm / Nominal defocus min: 1.2 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 115476
Initial angle assignmentType: RANDOM ASSIGNMENT
Final angle assignmentType: RANDOM ASSIGNMENT

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