EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	The structure and function of P5A-ATPases.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 9605, Year 2024
掲載日	2024年11月6日
著者	Ping Li / Viktoria Bågenholm / Per Hägglund / Karin Lindkvist-Petersson / Kaituo Wang / Pontus Gourdon /
PubMed 要旨	Endoplasmic reticulum (ER) membrane resident P5A-ATPases broadly affect protein biogenesis and quality control, and yet their molecular function remains debated. Here, we report cryo-EM structures of ...Endoplasmic reticulum (ER) membrane resident P5A-ATPases broadly affect protein biogenesis and quality control, and yet their molecular function remains debated. Here, we report cryo-EM structures of a P5A-ATPase, CtSpf1, covering multiple transport intermediates of the E1 → E1-ATP → E1P-ADP → E1P → E2P → E2.P → E2 → E1 cycle. In the E2P and E2.P states a cleft spans the entire membrane, holding a polypeptide cargo molecule. The cargo includes an ER luminal extension, pinpointed as the C-terminus in the E2.P state, which reenters the membrane in E2P. The E1 structure harbors a cytosol-facing cavity that is blocked by an insertion we refer to as the Plug-domain. The Plug-domain is nestled to key ATPase features and is displaced in the E1P-ADP and E1P states. Collectively, our findings are compatible with a broad range of proteins as cargo, with the P5A-ATPases serving a role in membrane removal of helices, although insertion/secretion cannot be excluded, as well as with a mechanistic role of the Plug-domain.
リンク	Nat Commun / PubMed:39505844 / PubMed Central
手法	EM (単粒子)
解像度	3.2 - 3.79 Å
構造データ	17039 8op3 EMDB-17039, PDB-8op3: Cryo-EM structure of P5A-ATPase CtSpf1 (E1 state) 手法: EM (単粒子) / 解像度: 3.41 Å 17040 8op4 EMDB-17040, PDB-8op4: Cryo-EM structure of P5A-ATPase CtSpf1 (E1-ATP state) 手法: EM (単粒子) / 解像度: 3.2 Å 17041 8op5 EMDB-17041, PDB-8op5: Cryo-EM structure of P5A-ATPase CtSpf1 (E1P-ADP state with membranous-feature bound) 手法: EM (単粒子) / 解像度: 3.47 Å 17042 8op6 EMDB-17042, PDB-8op6: Cryo-EM structure of P5A-ATPase CtSpf1 (E1P state with cytosolic-feature bound) 手法: EM (単粒子) / 解像度: 3.79 Å 17043 8op7 EMDB-17043, PDB-8op7: Cryo-EM structure of P5A-ATPase CtSpf1 ( E2P state with endogenous cargo bound) 手法: EM (単粒子) / 解像度: 3.5 Å 17044 8op8 EMDB-17044, PDB-8op8: Cryo-EM structure of P5A-ATPase CtSpf1 (E2.Pi state with endogenous helix cargo bound) 手法: EM (単粒子) / 解像度: 3.48 Å
化合物	ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP / AMP-PNP, エネルギー貯蔵分子類似体YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-ALF: TETRAFLUOROALUMINATE ION / テトラフルオロアルミナ-ト ChemComp-HOH: WATER ChemComp-BEF: BERYLLIUM TRIFLUORIDE ION ChemComp-CA: Unknown entry / カルシウムジカチオン
由来	thermochaetoides thermophila (菌類) saccharomyces cerevisiae (パン酵母)
キーワード	MEMBRANE PROTEIN / translocase