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- EMDB-17005: Pol I bound to extended and displaced DNA section - closed confor... -

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Basic information

Entry
Database: EMDB / ID: EMD-17005
TitlePol I bound to extended and displaced DNA section - closed conformation
Map data
Sample
  • Complex: Pol I bound to extended and displaced DNA section - closed conformation
    • Protein or peptide: DNA polymerase I
    • DNA: Template DNA
    • DNA: Extending Primer
    • DNA: Displaced primer
  • Ligand: MAGNESIUM ION
KeywordsDNA Polymerase I / Okazaki fragment maturation / DNA BINDING PROTEIN
Function / homology
Function and homology information


5'-3' exonuclease activity / 3'-5' exonuclease activity / base-excision repair / DNA-templated DNA replication / double-strand break repair / DNA-directed DNA polymerase / DNA-directed DNA polymerase activity / DNA replication / DNA repair / DNA binding ...5'-3' exonuclease activity / 3'-5' exonuclease activity / base-excision repair / DNA-templated DNA replication / double-strand break repair / DNA-directed DNA polymerase / DNA-directed DNA polymerase activity / DNA replication / DNA repair / DNA binding / cytoplasm / cytosol
Similarity search - Function
3'-5' exonuclease / DNA polymerase I-like, H3TH domain / 5'-3' exonuclease, C-terminal SAM fold / 5'-3' exonuclease, alpha-helical arch, N-terminal / 5'-3' exonuclease, N-terminal resolvase-like domain / 5'-3' exonuclease / 5'-3' exonuclease / DNA polymerase 1 / Helix-hairpin-helix motif, class 2 / Helix-hairpin-helix class 2 (Pol1 family) motifs ...3'-5' exonuclease / DNA polymerase I-like, H3TH domain / 5'-3' exonuclease, C-terminal SAM fold / 5'-3' exonuclease, alpha-helical arch, N-terminal / 5'-3' exonuclease, N-terminal resolvase-like domain / 5'-3' exonuclease / 5'-3' exonuclease / DNA polymerase 1 / Helix-hairpin-helix motif, class 2 / Helix-hairpin-helix class 2 (Pol1 family) motifs / 5'-3' exonuclease, C-terminal domain superfamily / 3'-5' exonuclease / 3'-5' exonuclease domain / DNA polymerase A / DNA polymerase family A / DNA-directed DNA polymerase, family A, conserved site / DNA polymerase family A signature. / DNA-directed DNA polymerase, family A, palm domain / DNA polymerase A domain / PIN-like domain superfamily / Ribonuclease H superfamily / Ribonuclease H-like superfamily / DNA/RNA polymerase superfamily
Similarity search - Domain/homology
Biological speciesEscherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria) / synthetic construct (others)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.3 Å
AuthorsBotto M / Borsellini A / Lamers MH
Funding support1 items
OrganizationGrant numberCountry
Other government
CitationJournal: Nat Struct Mol Biol / Year: 2023
Title: A four-point molecular handover during Okazaki maturation.
Authors: Margherita M Botto / Alessandro Borsellini / Meindert H Lamers /
Abstract: DNA replication introduces thousands of RNA primers into the lagging strand that need to be removed for replication to be completed. In Escherichia coli when the replicative DNA polymerase Pol IIIα ...DNA replication introduces thousands of RNA primers into the lagging strand that need to be removed for replication to be completed. In Escherichia coli when the replicative DNA polymerase Pol IIIα terminates at a previously synthesized RNA primer, DNA Pol I takes over and continues DNA synthesis while displacing the downstream RNA primer. The displaced primer is subsequently excised by an endonuclease, followed by the sealing of the nick by a DNA ligase. Yet how the sequential actions of Pol IIIα, Pol I polymerase, Pol I endonuclease and DNA ligase are coordinated is poorly defined. Here we show that each enzymatic activity prepares the DNA substrate for the next activity, creating an efficient four-point molecular handover. The cryogenic-electron microscopy structure of Pol I bound to a DNA substrate with both an upstream and downstream primer reveals how it displaces the primer in a manner analogous to the monomeric helicases. Moreover, we find that in addition to its flap-directed nuclease activity, the endonuclease domain of Pol I also specifically cuts at the RNA-DNA junction, thus marking the end of the RNA primer and creating a 5' end that is a suitable substrate for the ligase activity of LigA once all RNA has been removed.
History
DepositionApr 4, 2023-
Header (metadata) releaseAug 9, 2023-
Map releaseAug 9, 2023-
UpdateJul 2, 2025-
Current statusJul 2, 2025Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_17005.png

Downloads & links

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Map

FileDownload / File: emd_17005.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.87 Å/pix.
x 256 pix.
= 221.696 Å		0.87 Å/pix.
x 256 pix.
= 221.696 Å		0.87 Å/pix.
x 256 pix.
= 221.696 Å

Surface

Projections

Slices (1/3)

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.866 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0045
Minimum - Maximum-0.0077970317 - 0.02236765
Average (Standard dev.)0.000083705934 (±0.0007349592)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions256256256
Spacing256256256
CellA=B=C: 221.696 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_17005_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_17005_half_map_2.map
Projections & Slices
AxesZYX

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Slices (1/2)
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Histogram (log scale)

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Sample components

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Entire : Pol I bound to extended and displaced DNA section - closed confor...

EntireName: Pol I bound to extended and displaced DNA section - closed conformation
Components
  • Complex: Pol I bound to extended and displaced DNA section - closed conformation
    • Protein or peptide: DNA polymerase I
    • DNA: Template DNA
    • DNA: Extending Primer
    • DNA: Displaced primer
  • Ligand: MAGNESIUM ION

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Supramolecule #1: Pol I bound to extended and displaced DNA section - closed confor...

SupramoleculeName: Pol I bound to extended and displaced DNA section - closed conformation
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#4
Source (natural)Organism: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria)
Molecular weightTheoretical: 75 kDa/nm

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Macromolecule #1: DNA polymerase I

MacromoleculeName: DNA polymerase I / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed DNA polymerase
Source (natural)Organism: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria)
Molecular weightTheoretical: 67.991461 KDa
Recombinant expressionOrganism: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria)
SequenceString: GPHDNYVTIL DEETLKAWIA KLEKAPVFAF DTETDSLDNI SANLVGLSFA IEPGVAAYIP VAHDYLDAPD QISRERALEL LKPLLEDEK ALKVGQNLKY DRGILANYGI ELRGIAFDTM LESYILNSVA GRHDMDSLAE RWLKHKTITF EEIAGKGKNQ L TFNQIALE ...String:
GPHDNYVTIL DEETLKAWIA KLEKAPVFAF DTETDSLDNI SANLVGLSFA IEPGVAAYIP VAHDYLDAPD QISRERALEL LKPLLEDEK ALKVGQNLKY DRGILANYGI ELRGIAFDTM LESYILNSVA GRHDMDSLAE RWLKHKTITF EEIAGKGKNQ L TFNQIALE EAGRYAAEDA DVTLQLHLKM WPDLQKHKGP LNVFENIEMP LVPVLSRIER NGVKIDPKVL HNHSEELTLR LA ELEKKAH EIAGEEFNLS STKQLQTILF EKQGIKPLKK TPGGAPSTSE EVLEELALDY PLPKVILEYR GLAKLKSTYT DKL PLMINP KTGRVHTSYH QAVTATGRLS STDPNLQNIP VRNEEGRRIR QAFIAPEDYV IVSADYSQIE LRIMAHLSRD KGLL TAFAE GKDIHRATAA EVFGLPLETV TSEQRRSAKA INFGLIYGMS AFGLARQLNI PRKEAQKYMD LYFERYPGVL EYMER TRAQ AKEQGYVETL DGRRLYLPDI KSSNGARRAA AERAAINAPM QGTAADIIKR AMIAVDAWLQ AEQPRVRMIM QVHDEL VFE VHKDDVDAVA KQIHQLMENC TRLDVPLLVE VGSGENWDQA H

UniProtKB: DNA polymerase I

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Macromolecule #2: Template DNA

MacromoleculeName: Template DNA / type: dna / ID: 2 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 8.311362 KDa
SequenceString:
(DC)(DA)(DA)(DC)(DG)(DT)(DC)(DG)(DT)(DG) (DA)(DC)(DT)(DG)(DG)(DG)(DA)(DA)(DA)(DA) (DC)(DC)(DC)(DT)(DG)(DG)(DC)

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Macromolecule #3: Extending Primer

MacromoleculeName: Extending Primer / type: dna / ID: 3 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 5.48354 KDa
SequenceString:
(DG)(DC)(DC)(DA)(DG)(DG)(DG)(DT)(DT)(DT) (DT)(DC)(DC)(DC)(DA)(DG)(DT)(DC)

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Macromolecule #4: Displaced primer

MacromoleculeName: Displaced primer / type: dna / ID: 4 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 2.755823 KDa
SequenceString:
(DA)(DC)(DG)(DA)(DC)(DG)(DT)(DT)(DG)

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Macromolecule #5: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 5 / Number of copies: 1 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8.5
Sugar embeddingMaterial: water
GridModel: Quantifoil R0.6/1 / Material: COPPER / Mesh: 300 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 60 sec. / Pretreatment - Atmosphere: AIR / Pretreatment - Pressure: 2e-05 kPa
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 45.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

1kln

Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.3 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 65904
Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: MAXIMUM LIKELIHOOD

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