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- EMDB-16926: Human pseudouridine synthase 3 and tRNA-Gln -

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ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-16926
TitleHuman pseudouridine synthase 3 and tRNA-Gln
Map data
Sample
  • Complex: homodimer of PUS3 and tRNA-Gln complex
    • Complex: Pseudouridine synthase 3 (PUS3)
      • Protein or peptide: tRNA pseudouridine(38/39) synthase
    • Complex: tRNA-Gln
      • RNA: human tRNA-Gln (70-MER)
KeywordsRNA modification / pseudouridylation / tRNA / homodimer / RNA BINDING PROTEIN
Function / homology
Function and homology information


tRNA pseudouridine38/39 synthase / tRNA pseudouridine synthase activity / tRNA pseudouridine synthesis / mRNA pseudouridine synthesis / pseudouridine synthase activity / tRNA modification in the nucleus and cytosol / tRNA modification / RNA binding / nucleus / cytoplasm / cytosol
Similarity search - Function
Pseudouridine synthase Pus3-like / Pseudouridine synthase I, TruA / Pseudouridine synthase I, TruA, C-terminal / Pseudouridine synthase I, TruA, alpha/beta domain / tRNA pseudouridine synthase / Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminal / Pseudouridine synthase, catalytic domain superfamily
Similarity search - Domain/homology
tRNA pseudouridine(38/39) synthase
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.1 Å
AuthorsLin T-Y / Glatt S
Funding support Poland, European Union, Switzerland, 3 items
OrganizationGrant numberCountry
Polish National Science Centre2019/35/D/NZ1/02397 Poland
European Research Council (ERC)101001394European Union
Swiss National Science Foundation310030_184947 Switzerland
CitationJournal: Mol.Cell / Year: 2024
Title: Human pseudouridine synthase 3 (PUS3) and tRNA-Gln
Authors: Lin T-Y / Glatt S
History
DepositionMar 28, 2023-
Header (metadata) releaseJul 10, 2024-
Map releaseJul 10, 2024-
UpdateJul 10, 2024-
Current statusJul 10, 2024Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_16926.png

Downloads & links

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Map

FileDownload / File: emd_16926.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 0.86 Å
Density
Contour LevelBy AUTHOR: 0.25
Minimum - Maximum-1.4930806 - 2.363563
Average (Standard dev.)0.0011719924 (±0.05781334)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions256256256
Spacing256256256
CellA=B=C: 220.16 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_16926_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : homodimer of PUS3 and tRNA-Gln complex

EntireName: homodimer of PUS3 and tRNA-Gln complex
Components
  • Complex: homodimer of PUS3 and tRNA-Gln complex
    • Complex: Pseudouridine synthase 3 (PUS3)
      • Protein or peptide: tRNA pseudouridine(38/39) synthase
    • Complex: tRNA-Gln
      • RNA: human tRNA-Gln (70-MER)

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Supramolecule #1: homodimer of PUS3 and tRNA-Gln complex

SupramoleculeName: homodimer of PUS3 and tRNA-Gln complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Molecular weightTheoretical: 110 KDa

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Supramolecule #2: Pseudouridine synthase 3 (PUS3)

SupramoleculeName: Pseudouridine synthase 3 (PUS3) / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1
Source (natural)Organism: Homo sapiens (human)

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Supramolecule #3: tRNA-Gln

SupramoleculeName: tRNA-Gln / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #2
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: tRNA pseudouridine(38/39) synthase

MacromoleculeName: tRNA pseudouridine(38/39) synthase / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO / EC number: tRNA pseudouridine38/39 synthase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 55.704199 KDa
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: AMADNDTDRN QTEKLLKRVR ELEQEVQRLK KEQAKNKEDS NIRENSAGAG KTKRAFDFSA HGRRHVALRI AYMGWGYQGF ASQENTNNT IEEKLFEALT KTRLVESRQT SNYHRCGRTA KGVSAFGQVI SLDLRSQFPR GRDSEDFNVK EEANAAAEEI R YTHILNRV ...String:
AMADNDTDRN QTEKLLKRVR ELEQEVQRLK KEQAKNKEDS NIRENSAGAG KTKRAFDFSA HGRRHVALRI AYMGWGYQGF ASQENTNNT IEEKLFEALT KTRLVESRQT SNYHRCGRTA KGVSAFGQVI SLDLRSQFPR GRDSEDFNVK EEANAAAEEI R YTHILNRV LPPDIRILAW APVEPSFSAR FSCLERTYRY FFPRADLDIV TMDYAAQKYV GTHDFRNLCK MDVANGVINF QR TILSAQV QLVGQSPGEG RWQEPFQLCQ FEVTGQAFLY HQVRCMMAIL FLIGQGMEKP EIIDELLNIE KNPQKPQYSM AVE FPLVLY DCKFENVKWI YDQEAQEFNI THLQQLWANH AVKTHMLYSM LQGLDTVPVP CGIGPKMDGM TEWGNVKPSV IKQT SAFVE GVKMRTYKPL MDRPKCQGLE SRIQHFVRRG RIEHPHLFHE EETKAKRDCN DTLEEENTNL ETPTKRVCVD TEIKS II

UniProtKB: tRNA pseudouridine(38/39) synthase

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Macromolecule #2: human tRNA-Gln (70-MER)

MacromoleculeName: human tRNA-Gln (70-MER) / type: rna / ID: 2 / Number of copies: 2
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 24.047236 KDa
SequenceString:
GGCCCCAUGG UGUAAUGGUU AGCACUCUGG ACUUUGAAUC CAGCGAUCCG AGUUCAAAUC UCGGUGGGAC CUCCA

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.3 mg/mL
BufferpH: 7.5
GridModel: Quantifoil / Material: COPPER / Support film - Material: CARBON / Support film - topology: HOLEY ARRAY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 60 sec. / Pretreatment - Atmosphere: AIR / Details: 8 mA
VitrificationCryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number grids imaged: 1 / Number real images: 8320 / Average electron dose: 40.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 1.8 µm / Nominal defocus min: 0.9 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 482246
Startup modelType of model: INSILICO MODEL / In silico model: alphafold2
Final reconstructionNumber classes used: 1 / Resolution.type: BY AUTHOR / Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4.1) / Number images used: 147307
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 4.1)
Final angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: cryoSPARC (ver. 4.1)
Final 3D classificationNumber classes: 3 / Avg.num./class: 16000 / Software - Name: cryoSPARC (ver. 4.1)
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelChain - Source name: AlphaFold / Chain - Initial model type: in silico model
RefinementProtocol: AB INITIO MODEL
Output model

PDB-8okd:
Human pseudouridine synthase 3 and tRNA-Gln

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