EMDB-16452: Human spliceosomal PM5 C* complex

Basic information

Entry

Database: EMDB / ID: EMD-16452

• Title: Human spliceosomal PM5 C* complex
• Map data: map of the human spliceosomal PM5 C* complex

Sample

• Complex: human spliceosomal C* complex assembled on PM5 pre-mRNA
  • RNA: x 5 types
  • Protein or peptide: x 48 types
• Ligand: x 6 types

• Keywords: spliceosome / nucleus / splicing

Function / homology

Function:

regulation of homologous chromosome segregation / endoplasmic reticulum membrane organization / second spliceosomal transesterification activity / exon-exon junction subcomplex mago-y14 / post-spliceosomal complex / negative regulation of selenocysteine incorporation / regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / spliceosomal complex disassembly / cellular response to selenite ion / negative regulation of nitric-oxide synthase activity / selenocysteine insertion sequence binding / exon-exon junction complex / NOSIP mediated eNOS trafficking / pre-mRNA 3'-splice site binding / granulocyte differentiation / snRNP binding / post-mRNA release spliceosomal complex / U2 snRNP binding / regulation of retinoic acid receptor signaling pathway / negative regulation of toll-like receptor signaling pathway / U7 snRNA binding / histone pre-mRNA DCP binding / 3'-5' RNA helicase activity / regulation of translation at postsynapse, modulating synaptic transmission / U7 snRNP / generation of catalytic spliceosome for first transesterification step / renal system process / negative regulation of excitatory postsynaptic potential / histone pre-mRNA 3'end processing complex / alternative mRNA splicing, via spliceosome / endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / cis assembly of pre-catalytic spliceosome / regulation of vitamin D receptor signaling pathway / Z-decay: degradation of maternal mRNAs by zygotically expressed factors / negative regulation of catalytic activity / SLBP independent Processing of Histone Pre-mRNAs / SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs / negative regulation of interleukin-8 production / regulation of mRNA processing / negative regulation of lipopolysaccharide-mediated signaling pathway / Deadenylation of mRNA / spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) / embryonic brain development / negative regulation of phosphorylation / protein methylation / U12-type spliceosomal complex / methylosome / STING mediated induction of host immune responses / pre-mRNA binding / 7-methylguanosine cap hypermethylation / negative regulation of interferon-beta production / nuclear retinoic acid receptor binding / U2-type catalytic step 1 spliceosome / pICln-Sm protein complex / U1 snRNP binding / Prp19 complex / RNA splicing, via transesterification reactions / poly(A) binding / positive regulation of androgen receptor activity / M-decay: degradation of maternal mRNAs by maternally stored factors / RNA stem-loop binding / spliceosomal tri-snRNP complex / mRNA 3'-end processing / small nuclear ribonucleoprotein complex / P granule / sno(s)RNA-containing ribonucleoprotein complex / ATP-dependent activity, acting on RNA / SMN-Sm protein complex / embryonic cranial skeleton morphogenesis / mRNA cis splicing, via spliceosome / U2-type spliceosomal complex / telomerase RNA binding / IRF3-mediated induction of type I IFN / U2-type precatalytic spliceosome / positive regulation of mRNA splicing, via spliceosome / telomerase holoenzyme complex / C2H2 zinc finger domain binding / regulation of mRNA splicing, via spliceosome / U2-type prespliceosome assembly / commitment complex / U2-type catalytic step 2 spliceosome / positive regulation by host of viral transcription / U4 snRNP / positive regulation of vitamin D receptor signaling pathway / Transport of Mature mRNA derived from an Intron-Containing Transcript / Notch binding / U2 snRNP / nuclear vitamin D receptor binding / Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells / RNA Polymerase II Transcription Termination / RUNX3 regulates NOTCH signaling / U1 snRNP / positive regulation of alpha-beta T cell differentiation / NOTCH4 Intracellular Domain Regulates Transcription / U2-type prespliceosome / ubiquitin-ubiquitin ligase activity / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / exploration behavior / K63-linked polyubiquitin modification-dependent protein binding / WD40-repeat domain binding

Domain/homology:

XAP5 protein / Nitric oxide synthase-interacting protein / Nuclear protein DGCR14/ESS-2 / STEEP-like / Nitric oxide synthase-interacting protein, zinc-finger / XAP5, circadian clock regulator C-terminal domain / Nuclear protein Es2 / Zinc-finger of nitric oxide synthase-interacting protein / Telomere length and silencing protein 1 / Hepatocellular carcinoma-associated antigen 59 / Sde2, N-terminal ubiquitin domain / Silencing defective 2 N-terminal ubiquitin domain / NF-kappa-B-activating protein, C-terminal / NF-kappa-B-activating protein / DDX41, DEAD-box helicase domain / NF-kappa-B-activating protein C-terminal domain / NF-kappa-B-activating protein / Protein FAM32A / Cactin, central domain / Cactin, C-terminal / FAM32A / Cactus-binding C-terminus of cactin protein / Conserved mid region of cactin / PRKR-interacting protein 1 / Protein of unknown function (DUF1168) / Pre-mRNA-splicing factor SLU7 domain / Pre-mRNA-splicing factor SLU7 / Pre-mRNA splicing Prp18-interacting factor / DHX8/ Prp22, DEXH-box helicase domain / Mago nashi protein / RNA-binding motif protein 8 / RBM8, RNA recognition motif / Mago nashi superfamily / Mago nashi protein / Pre-mRNA-splicing factor Isy1 / Pre-mRNA-splicing factor Isy1 superfamily / Isy1-like splicing family / : / : / : / : / Intron-binding protein aquarius, beta-barrel / Intron-binding protein aquarius insert domain / : / Pre-mRNA-splicing factor SPF27 / Breast carcinoma amplified sequence 2 (BCAS2) / CWF11 family / Intron-binding protein aquarius, N-terminal / Intron-binding protein aquarius N-terminal / Peptidyl-prolyl cis-trans isomerase E / Peptidyl-prolyl cis-trans isomerase E, RNA recognition motif / mRNA splicing factor SYF2 / SYF2 splicing factor / : / Replication stress response SDE2 C-terminal / Cactus-binding C-terminus of cactin protein / U2 small nuclear ribonucleoprotein B'', RNA recognition motif 2 / U2 small nuclear ribonucleoprotein B'', RNA recognition motif 1 / Cyclophilin-type peptidyl-prolyl cis-trans isomerase, cyclophilin A-like / Helix hairpin bin domain superfamily / mRNA splicing factor Cwf21 domain / cwf21 domain / Pre-mRNA-processing factor 17 / : / STL11, N-terminal / : / Pre-mRNA-splicing factor 19 / Pre-mRNA-processing factor 19 / DNA2/NAM7 helicase, helicase domain / DNA2/NAM7-like helicase / Prp19/Pso4-like / AAA domain / WD repeat Prp46/PLRG1-like / U-box domain / BUD31/G10-related, conserved site / : / : / G10 protein signature 1. / G10 protein signature 2. / SKI-interacting protein SKIP, SNW domain / SKI-interacting protein, SKIP / SKIP/SNW domain / Myb-like DNA-binding domain / Pre-mRNA-splicing factor Cwf15/Cwc15 / HAT (Half-A-TPR) repeat / Cwf15/Cwc15 cell cycle control protein / Pre-mRNA-splicing factor Cwc2/Slt11 / G10 protein / Pre-mRNA-splicing factor BUD31 / Pre-mRNA splicing factor component Cdc5p/Cef1, C-terminal / pre-mRNA splicing factor component / Small ribonucleoprotein associated, SmB/SmN / Initiation factor eIF-4 gamma, MA3 / MA3 domain / MI domain profile. / Domain in DAP-5, eIF4G, MA-3 and other proteins. / MIF4G domain / Spt5 C-terminal domain / Spt5 C-terminal nonapeptide repeat binding Spt4 / Small nuclear ribonucleoprotein D1

Component:

Pleiotropic regulator 1 / RNA helicase aquarius / Pre-mRNA-processing factor 17 / U5 small nuclear ribonucleoprotein 200 kDa helicase / Pre-mRNA-splicing factor SPF27 / Pre-mRNA-splicing factor SLU7 / Pre-mRNA-splicing factor SYF2 / U2 small nuclear ribonucleoprotein B'' / U2 small nuclear ribonucleoprotein A' / Small nuclear ribonucleoprotein-associated proteins B and B' / Eukaryotic initiation factor 4A-III / Protein BUD31 homolog / Protein mago nashi homolog / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein F / Small nuclear ribonucleoprotein G / Small nuclear ribonucleoprotein Sm D1 / Small nuclear ribonucleoprotein Sm D2 / Small nuclear ribonucleoprotein Sm D3 / SNW domain-containing protein 1 / Protein FAM50A / ATP-dependent RNA helicase DHX8 / 116 kDa U5 small nuclear ribonucleoprotein component / Splicing regulator SDE2 / Pre-mRNA-processing-splicing factor 8 / NF-kappa-B-activating protein / Splicing factor Cactin / Splicing factor ESS-2 homolog / U5 small nuclear ribonucleoprotein 40 kDa protein / Cell division cycle 5-like protein / Crooked neck-like protein 1 / Peptidyl-prolyl cis-trans isomerase-like 3 / STING ER exit protein / PRKR-interacting protein 1 / Pre-mRNA-splicing factor CWC22 homolog / Pre-mRNA-splicing factor SYF1 / Pre-mRNA-splicing factor RBM22 / Splicing factor C9orf78 / Spliceosome-associated protein CWC15 homolog / Probable ATP-dependent RNA helicase DDX41 / Pre-mRNA-splicing factor ISY1 homolog / Pre-mRNA-processing factor 19 / Peptidyl-prolyl cis-trans isomerase E / Serine/arginine repetitive matrix protein 2 / Nitric oxide synthase-interacting protein / Peptidyl-prolyl cis-trans isomerase-like 1 / Protein FAM32A / RNA-binding protein 8A

• Biological species: Homo sapiens (human) / human (human)
• Method: single particle reconstruction / cryo EM / Resolution: 2.8 Å
• Authors: Dybkov O / Kastner B / Luehrmann R

Funding support

Germany, 1 items

Organization	Grant number	Country
Max Planck Society		Germany

• Citation: Journal: Sci Adv / Year: 2023; Title: Regulation of 3' splice site selection after step 1 of splicing by spliceosomal C* proteins.; Authors: Olexandr Dybkov / Marco Preußner / Leyla El Ayoubi / Vivi-Yun Feng / Caroline Harnisch / Kilian Merz / Paula Leupold / Peter Yudichev / Dmitry E Agafonov / Cindy L Will / Cyrille Girard / Christian Dienemann / Henning Urlaub / Berthold Kastner / Florian Heyd / Reinhard Lührmann / ; Abstract: Alternative precursor messenger RNA splicing is instrumental in expanding the proteome of higher eukaryotes, and changes in 3' splice site (3'ss) usage contribute to human disease. We demonstrate by small interfering RNA-mediated knockdowns, followed by RNA sequencing, that many proteins first recruited to human C* spliceosomes, which catalyze step 2 of splicing, regulate alternative splicing, including the selection of alternatively spliced NAGNAG 3'ss. Cryo-electron microscopy and protein cross-linking reveal the molecular architecture of these proteins in C* spliceosomes, providing mechanistic and structural insights into how they influence 3'ss usage. They further elucidate the path of the 3' region of the intron, allowing a structure-based model for how the C* spliceosome potentially scans for the proximal 3'ss. By combining biochemical and structural approaches with genome-wide functional analyses, our studies reveal widespread regulation of alternative 3'ss usage after step 1 of splicing and the likely mechanisms whereby C* proteins influence NAGNAG 3'ss choices.

History

Deposition	Jan 12, 2023	-
Header (metadata) release	Jul 12, 2023	-
Map release	Jul 12, 2023	-
Update	Jul 12, 2023	-
Current status	Jul 12, 2023	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_16452.map.gz / Format: CCP4 / Size: 744.3 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: map of the human spliceosomal PM5 C* complex
• Voxel size: X=Y=Z: 1.05 Å

Density

Contour Level	By AUTHOR: 0.115
Minimum - Maximum	-0.35913646 - 1.2596285
Average (Standard dev.)	0.0012087063 (±0.026175821)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 580 580 580 Spacing 580 580 580
Cell	A=B=C: 609.0 Å α=β=γ: 90.0 °

Supplemental data

Additional map: locally filtered map of the human spliceosomal PM5 C* complex

• File: emd_16452_additional_1.map
• Annotation: locally filtered map of the human spliceosomal PM5 C* complex

Half map: half map A

• File: emd_16452_half_map_1.map
• Annotation: half map A

Half map: half map B

• File: emd_16452_half_map_2.map
• Annotation: half map B

Sample components

Entire : human spliceosomal C* complex assembled on PM5 pre-mRNA

• Entire: Name: human spliceosomal C* complex assembled on PM5 pre-mRNA

Components

• Complex: human spliceosomal C* complex assembled on PM5 pre-mRNA
  • RNA: U2 snRNAU2 spliceosomal RNA
  • Protein or peptide: Splicing factor ESS-2 homolog
  • RNA: U5 snRNAU5 spliceosomal RNA
  • RNA: U6 snRNAU6 spliceosomal RNA
  • Protein or peptide: Eukaryotic initiation factor 4A-III
  • Protein or peptide: RNA-binding protein 8A
  • Protein or peptide: Protein mago nashi homolog
  • Protein or peptide: Pre-mRNA-processing-splicing factor 8
  • Protein or peptide: U5 small nuclear ribonucleoprotein 200 kDa helicase
  • Protein or peptide: 116 kDa U5 small nuclear ribonucleoprotein component
  • Protein or peptide: Peptidyl-prolyl cis-trans isomerase-like 3
  • Protein or peptide: Probable ATP-dependent RNA helicase DDX41
  • Protein or peptide: Peptidyl-prolyl cis-trans isomerase E
  • Protein or peptide: Protein FAM50A
  • Protein or peptide: Pre-mRNA-splicing factor ISY1 homolog
  • Protein or peptide: Nitric oxide synthase-interacting protein
  • Protein or peptide: Splicing factor C9orf78
  • Protein or peptide: PRKR-interacting protein 1
  • RNA: PM5 5' Exon
  • Protein or peptide: Splicing factor Cactin
  • Protein or peptide: Protein FAM32A
  • Protein or peptide: Pre-mRNA-splicing factor CWC22 homolog
  • RNA: PM5 Intron
  • Protein or peptide: Pleiotropic regulator 1
  • Protein or peptide: SNW domain-containing protein 1
  • Protein or peptide: Protein BUD31 homolog
  • Protein or peptide: Pre-mRNA-splicing factor RBM22
  • Protein or peptide: U5 small nuclear ribonucleoprotein 40 kDa protein
  • Protein or peptide: Cell division cycle 5-like protein
  • Protein or peptide: Spliceosome-associated protein CWC15 homolog
  • Protein or peptide: Serine/arginine repetitive matrix protein 2
  • Protein or peptide: Crooked neck-like protein 1
  • Protein or peptide: Pre-mRNA-splicing factor SYF1
  • Protein or peptide: Intron-binding protein aquarius
  • Protein or peptide: ATP-dependent RNA helicase DHX8
  • Protein or peptide: U2 small nuclear ribonucleoprotein A'
  • Protein or peptide: U2 small nuclear ribonucleoprotein B''
  • Protein or peptide: NF-kappa-B-activating protein
  • Protein or peptide: Small nuclear ribonucleoprotein Sm D2
  • Protein or peptide: Small nuclear ribonucleoprotein-associated proteins B and B'
  • Protein or peptide: Small nuclear ribonucleoprotein Sm D1
  • Protein or peptide: Small nuclear ribonucleoprotein Sm D3
  • Protein or peptide: Small nuclear ribonucleoprotein E
  • Protein or peptide: Small nuclear ribonucleoprotein F
  • Protein or peptide: Small nuclear ribonucleoprotein G
  • Protein or peptide: Pre-mRNA-splicing factor SLU7
  • Protein or peptide: Peptidyl-prolyl cis-trans isomerase-like 1
  • Protein or peptide: STING ER exit protein
  • Protein or peptide: Pre-mRNA-processing factor 17
  • Protein or peptide: Pre-mRNA-splicing factor SPF27
  • Protein or peptide: Pre-mRNA-processing factor 19
  • Protein or peptide: Pre-mRNA-splicing factor SYF2
  • Protein or peptide: Splicing regulator SDE2
• Ligand: POTASSIUM IONPotassium
• Ligand: MAGNESIUM ION
• Ligand: ADENOSINE-5'-TRIPHOSPHATE
• Ligand: INOSITOL HEXAKISPHOSPHATEPhytic acid
• Ligand: GUANOSINE-5'-TRIPHOSPHATEGuanosine triphosphate
• Ligand: ZINC ION

Supramolecule #1: human spliceosomal C* complex assembled on PM5 pre-mRNA

• Supramolecule: Name: human spliceosomal C* complex assembled on PM5 pre-mRNA; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#53
• Source (natural): Organism: Homo sapiens (human)

Macromolecule #1: U2 snRNA

• Macromolecule: Name: U2 snRNA / type: rna / ID: 1 / Number of copies: 1
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 60.186445 KDa

Sequence

String:

AUCGCUUCUC GGCCUUUUGG CUAAGAUCAA GUGUAGUAUC UGUUCUUAUC AGUUUAAUAU CUGAUACGUC CUCUAUCCGA GGACAAUAU AUUAAAUGGA UUUUUGGAGC AGGGAGAUGG AAUAGGAGCU UGCUCCGUCC ACUCCACGCA UCGACCUGGU A UUGCAGUA CCUCCAGGAA CGGUGCACCC

GENBANK: GENBANK: X02665.1

Macromolecule #3: U5 snRNA

• Macromolecule: Name: U5 snRNA / type: rna / ID: 3 / Number of copies: 1
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 36.908668 KDa

Sequence

String:

AUACUCUGGU UUCUCUUCAG AUCGCAUAAA UCUUUCGCCU UUUACUAAAG AUUUCCGUGG AGAGGAACAA CUCUGAGUCU UAACCCAAU UUUUUGAGCC UUGCCUUGGC AAGGCUA

GENBANK: GENBANK: X01691.1

Macromolecule #4: U6 snRNA

• Macromolecule: Name: U6 snRNA / type: rna / ID: 4 / Number of copies: 1
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 34.09827 KDa

Sequence

String:

GUGCUCGCUU CGGCAGCACA UAUACUAAAA UUGGAACGAU ACAGAGAAGA UUAGCAUGGC CCCUGCGCAA GGAUGACACG CAAAUUCGU GAAGCGUUCC AUAUUUU

GENBANK: GENBANK: NR_004394.1

Macromolecule #19: PM5 5' Exon

• Macromolecule: Name: PM5 5' Exon / type: rna / ID: 19 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 78.463805 KDa

Sequence

String:

GAAUACAAGC UCAUCCGAUA UCCGUACACC AUCAGGGUAC GAGCUAGCCC AUGGCGUACA CCAUCAGGGU ACGACUAGUA GAUCUCGUA CACCAUCAGG GUACGGAAUU CUCUAGAGUC GAGGAGGACA UCUCAGCAAA AGAGAAGCUG CUGCGGGCGU C GGAGGACG AGCGGGACCG GGUGCUGGAG GAGCUGCACA AGGCAGAGGA CAGCCUGCUG GCUGCCGACG AGACCGCCGC CA AG

Macromolecule #23: PM5 Intron

• Macromolecule: Name: PM5 Intron / type: rna / ID: 23 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 74.973766 KDa

Sequence

String:

GUAUGUAUCA AGCUUACAAG ACAGCUUUAA GGAGACCAAU AGAAACUGGG CAUGUGGAGA CAGAGAAGAC UCUUGGCCUC GAGAAACCU GUAACUGGAA UGUGUGUGGA GUGUGACUGA UAGAACACUA CCUGAUUCUU AUGUAUUUAC UGACCUGUGU U UUUUUGCU ACUUUUUUUC UUUUCUCCCC UUCCCCUUUC CCUAUUUUUU UUCUUGCCCU GAUCCGGAAU UUGGAUC

Macromolecule #2: Splicing factor ESS-2 homolog

• Macromolecule: Name: Splicing factor ESS-2 homolog / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 52.639953 KDa

Sequence

String:

METPGASASS LLLPAASRPP RKREAGEAGA ATSKQRVLDE EEYIEGLQTV IQRDFFPDVE KLQAQKEYLE AEENGDLERM RQIAIKFGS ALGKMSREPP PPYVTPATFE TPEVHAGTGV VGNKPRPRGR GLEDGEAGEE EEKEPLPSLD VFLSRYTSED N ASFQEIME VAKERSRARH AWLYQAEEEF EKRQKDNLEL PSAEHQAIES SQASVETWKY KAKNSLMYYP EGVPDEEQLF KK PRQVVHK NTRFLRDPFS QALSRCQLQQ AAALNAQHKQ GKVGPDGKEL IPQESPRVGG FGFVATPSPA PGVNESPMMT WGE VENTPL RVEGSETPYV DRTPGPAFKI LEPGRRERLG LKMANEAAAK NRAKKQEALR RVTENLASLT PKGLSPAMSP ALQR LVSRT ASKYTDRALR ASYTPSPARS THLKTPASGL QTPTSTPAPG SATRTPLTQD PASITDNLLQ LPARRKASDF F

UniProtKB: Splicing factor ESS-2 homolog

Macromolecule #5: Eukaryotic initiation factor 4A-III

• Macromolecule: Name: Eukaryotic initiation factor 4A-III / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 46.930961 KDa

Sequence

String:

MATTATMATS GSARKRLLKE EDMTKVEFET SEEVDVTPTF DTMGLREDLL RGIYAYGFEK PSAIQQRAIK QIIKGRDVIA QSQSGTGKT ATFSISVLQC LDIQVRETQA LILAPTRELA VQIQKGLLAL GDYMNVQCHA CIGGTNVGED IRKLDYGQHV V AGTPGRVF DMIRRRSLRT RAIKMLVLDE ADEMLNKGFK EQIYDVYRYL PPATQVVLIS ATLPHEILEM TNKFMTDPIR IL VKRDELT LEGIKQFFVA VEREEWKFDT LCDLYDTLTI TQAVIFCNTK RKVDWLTEKM REANFTVSSM HGDMPQKERE SIM KEFRSG ASRVLISTDV WARGLDVPQV SLIINYDLPN NRELYIHRIG RSGRYGRKGV AINFVKNDDI RILRDIEQYY STQI DEMPM NVADLI

UniProtKB: Eukaryotic initiation factor 4A-III

Macromolecule #6: RNA-binding protein 8A

• Macromolecule: Name: RNA-binding protein 8A / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 19.92507 KDa

Sequence

String:

MADVLDLHEA GGEDFAMDED GDESIHKLKE KAKKRKGRGF GSEEGSRARM REDYDSVEQD GDEPGPQRSV EGWILFVTGV HEEATEEDI HDKFAEYGEI KNIHLNLDRR TGYLKGYTLV EYETYKEAQA AMEGLNGQDL MGQPISVDWC FVRGPPKGKR R GGRRRSRS PDRRRR

UniProtKB: RNA-binding protein 8A

Macromolecule #7: Protein mago nashi homolog

• Macromolecule: Name: Protein mago nashi homolog / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 17.189625 KDa

Sequence

String:

MESDFYLRYY VGHKGKFGHE FLEFEFRPDG KLRYANNSNY KNDVMIRKEA YVHKSVMEEL KRIIDDSEIT KEDDALWPPP DRVGRQELE IVIGDEHISF TTSKIGSLID VNQSKDPEGL RVFYYLVQDL KCLVFSLIGL HFKIKPI

UniProtKB: Protein mago nashi homolog

Macromolecule #8: Pre-mRNA-processing-splicing factor 8

• Macromolecule: Name: Pre-mRNA-processing-splicing factor 8 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 273.97425 KDa

Sequence

String:

MAGVFPYRGP GNPVPGPLAP LPDYMSEEKL QEKARKWQQL QAKRYAEKRK FGFVDAQKED MPPEHVRKII RDHGDMTNRK FRHDKRVYL GALKYMPHAV LKLLENMPMP WEQIRDVPVL YHITGAISFV NEIPWVIEPV YISQWGSMWI MMRREKRDRR H FKRMRFPP FDDEEPPLDY ADNILDVEPL EAIQLELDPE EDAPVLDWFY DHQPLRDSRK YVNGSTYQRW QFTLPMMSTL YR LANQLLT DLVDDNYFYL FDLKAFFTSK ALNMAIPGGP KFEPLVRDIN LQDEDWNEFN DINKIIIRQP IRTEYKIAFP YLY NNLPHH VHLTWYHTPN VVFIKTEDPD LPAFYFDPLI NPISHRHSVK SQEPLPDDDE EFELPEFVEP FLKDTPLYTD NTAN GIALL WAPRPFNLRS GRTRRALDIP LVKNWYREHC PAGQPVKVRV SYQKLLKYYV LNALKHRPPK AQKKRYLFRS FKATK FFQS TKLDWVEVGL QVCRQGYNML NLLIHRKNLN YLHLDYNFNL KPVKTLTTKE RKKSRFGNAF HLCREVLRLT KLVVDS HVQ YRLGNVDAFQ LADGLQYIFA HVGQLTGMYR YKYKLMRQIR MCKDLKHLIY YRFNTGPVGK GPGCGFWAAG WRVWLFF MR GITPLLERWL GNLLARQFEG RHSKGVAKTV TKQRVESHFD LELRAAVMHD ILDMMPEGIK QNKARTILQH LSEAWRCW K ANIPWKVPGL PTPIENMILR YVKAKADWWT NTAHYNRERI RRGATVDKTV CKKNLGRLTR LYLKAEQERQ HNYLKDGPY ITAEEAVAVY TTTVHWLESR RFSPIPFPPL SYKHDTKLLI LALERLKEAY SVKSRLNQSQ REELGLIEQA YDNPHEALSR IKRHLLTQR AFKEVGIEFM DLYSHLVPVY DVEPLEKITD AYLDQYLWYE ADKRRLFPPW IKPADTEPPP LLVYKWCQGI N NLQDVWET SEGECNVMLE SRFEKMYEKI DLTLLNRLLR LIVDHNIADY MTAKNNVVIN YKDMNHTNSY GIIRGLQFAS FI VQYYGLV MDLLVLGLHR ASEMAGPPQM PNDFLSFQDI ATEAAHPIRL FCRYIDRIHI FFRFTADEAR DLIQRYLTEH PDP NNENIV GYNNKKCWPR DARMRLMKHD VNLGRAVFWD IKNRLPRSVT TVQWENSFVS VYSKDNPNLL FNMCGFECRI LPKC RTSYE EFTHKDGVWN LQNEVTKERT AQCFLRVDDE SMQRFHNRVR QILMASGSTT FTKIVNKWNT ALIGLMTYFR EAVVN TQEL LDLLVKCENK IQTRIKIGLN SKMPSRFPPV VFYTPKELGG LGMLSMGHVL IPQSDLRWSK QTDVGITHFR SGMSHE EDQ LIPNLYRYIQ PWESEFIDSQ RVWAEYALKR QEAIAQNRRL TLEDLEDSWD RGIPRINTLF QKDRHTLAYD KGWRVRT DF KQYQVLKQNP FWWTHQRHDG KLWNLNNYRT DMIQALGGVE GILEHTLFKG TYFPTWEGLF WEKASGFEES MKWKKLTN A QRSGLNQIPN RRFTLWWSPT INRANVYVGF QVQLDLTGIF MHGKIPTLKI SLIQIFRAHL WQKIHESIVM DLCQVFDQE LDALEIETVQ KETIHPRKSY KMNSSCADIL LFASYKWNVS RPSLLADSKD VMDSTTTQKY WIDIQLRWGD YDSHDIERYA RAKFLDYTT DNMSIYPSPT GVLIAIDLAY NLHSAYGNWF PGSKPLIQQA MAKIMKANPA LYVLRERIRK GLQLYSSEPT E PYLSSQNY GELFSNQIIW FVDDTNVYRV TIHKTFEGNL TTKPINGAIF IFNPRTGQLF LKIIHTSVWA GQKRLGQLAK WK TAEEVAA LIRSLPVEEQ PKQIIVTRKG MLDPLEVHLL DFPNIVIKGS ELQLPFQACL KVEKFGDLIL KATEPQMVLF NLY DDWLKT ISSYTAFSRL ILILRALHVN NDRAKVILKP DKTTITEPHH IWPTLTDEEW IKVEVQLKDL ILADYGKKNN VNVA SLTQS EIRDIILGME ISAPSQQRQQ IAEIEKQTKE QSQLTATQTR TVNKHGDEII TSTTSNYETQ TFSSKTEWRV RAISA ANLH LRTNHIYVSS DDIKETGYTY ILPKNVLKKF ICISDLRAQI AGYLYGVSPP DNPQVKEIRC IVMVPQWGTH QTVHLP GQL PQHEYLKEME PLGWIHTQPN ESPQLSPQDV TTHAKIMADN PSWDGEKTII ITCSFTPGSC TLTAYKLTPS GYEWGRQ NT DKGNNPKGYL PSHYERVQML LSDRFLGFFM VPAQSSWNYN FMGVRHDPNM KYELQLANPK EFYHEVHRPS HFLNFALL Q EGEVYSADRE DLYA

UniProtKB: Pre-mRNA-processing-splicing factor 8

Macromolecule #9: U5 small nuclear ribonucleoprotein 200 kDa helicase

• Macromolecule: Name: U5 small nuclear ribonucleoprotein 200 kDa helicase / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 244.823422 KDa

Sequence

String:

MADVTARSLQ YEYKANSNLV LQADRSLIDR TRRDEPTGEV LSLVGKLEGT RMGDKAQRTK PQMQEERRAK RRKRDEDRHD INKMKGYTL LSEGIDEMVG IIYKPKTKET RETYEVLLSF IQAALGDQPR DILCGAADEV LAVLKNEKLR DKERRKEIDL L LGQTDDTR YHVLVNLGKK ITDYGGDKEI QNMDDNIDET YGVNVQFESD EEEGDEDVYG EVREEASDDD MEGDEAVVRC TL SANLVAS GELMSSKKKD LHPRDIDAFW LQRQLSRFYD DAIVSQKKAD EVLEILKTAS DDRECENQLV LLLGFNTFDF IKV LRQHRM MILYCTLLAS AQSEAEKERI MGKMEADPEL SKFLYQLHET EKEDLIREER SRRERVRQSR MDTDLETMDL DQGG EALAP RQVLDLEDLV FTQGSHFMAN KRCQLPDGSF RRQRKGYEEV HVPALKPKPF GSEEQLLPVE KLPKYAQAGF EGFKT LNRI QSKLYRAALE TDENLLLCAP TGAGKTNVAL MCMLREIGKH INMDGTINVD DFKIIYIAPM RSLVQEMVGS FGKRLA TYG ITVAELTGDH QLCKEEISAT QIIVCTPEKW DIITRKGGER TYTQLVRLII LDEIHLLHDD RGPVLEALVA RAIRNIE MT QEDVRLIGLS ATLPNYEDVA TFLRVDPAKG LFYFDNSFRP VPLEQTYVGI TEKKAIKRFQ IMNEIVYEKI MEHAGKNQ V LVFVHSRKET GKTARAIRDM CLEKDTLGLF LREGSASTEV LRTEAEQCKN LELKDLLPYG FAIHHAGMTR VDRTLVEDL FADKHIQVLV STATLAWGVN LPAHTVIIKG TQVYSPEKGR WTELGALDIL QMLGRAGRPQ YDTKGEGILI TSHGELQYYL SLLNQQLPI ESQMVSKLPD MLNAEIVLGN VQNAKDAVNW LGYAYLYIRM LRSPTLYGIS HDDLKGDPLL DQRRLDLVHT A ALMLDKNN LVKYDKKTGN FQVTELGRIA SHYYITNDTV QTYNQLLKPT LSEIELFRVF SLSSEFKNIT VREEEKLELQ KL LERVPIP VKESIEEPSA KINVLLQAFI SQLKLEGFAL MADMVYVTQS AGRLMRAIFE IVLNRGWAQL TDKTLNLCKM IDK RMWQSM CPLRQFRKLP EEVVKKIEKK NFPFERLYDL NHNEIGELIR MPKMGKTIHK YVHLFPKLEL SVHLQPITRS TLKV ELTIT PDFQWDEKVH GSSEAFWILV EDVDSEVILH HEYFLLKAKY AQDEHLITFF VPVFEPLPPQ YFIRVVSDRW LSCET QLPV SFRHLILPEK YPPPTELLDL QPLPVSALRN SAFESLYQDK FPFFNPIQTQ VFNTVYNSDD NVFVGAPTGS GKTICA EFA ILRMLLQSSE GRCVYITPME ALAEQVYMDW YEKFQDRLNK KVVLLTGETS TDLKLLGKGN IIISTPEKWD ILSRRWK QR KNVQNINLFV VDEVHLIGGE NGPVLEVICS RMRYISSQIE RPIRIVALSS SLSNAKDVAH WLGCSATSTF NFHPNVRP V PLELHIQGFN ISHTQTRLLS MAKPVYHAIT KHSPKKPVIV FVPSRKQTRL TAIDILTTCA ADIQRQRFLH CTEKDLIPY LEKLSDSTLK ETLLNGVGYL HEGLSPMERR LVEQLFSSGA IQVVVASRSL CWGMNVAAHL VIIMDTQYYN GKIHAYVDYP IYDVLQMVG HANRPLQDDE GRCVIMCQGS KKDFFKKFLY EPLPVESHLD HCMHDHFNAE IVTKTIENKQ DAVDYLTWTF L YRRMTQNP NYYNLQGISH RHLSDHLSEL VEQTLSDLEQ SKCISIEDEM DVAPLNLGMI AAYYYINYTT IELFSMSLNA KT KVRGLIE IISNAAEYEN IPIRHHEDNL LRQLAQKVPH KLNNPKFNDP HVKTNLLLQA HLSRMQLSAE LQSDTEEILS KAI RLIQAC VDVLSSNGWL SPALAAMELA QMVTQAMWSK DSYLKQLPHF TSEHIKRCTD KGVESVFDIM EMEDEERNAL LQLT DSQIA DVARFCNRYP NIELSYEVVD KDSIRSGGPV VVLVQLEREE EVTGPVIAPL FPQKREEGWW VVIGDAKSNS LISIK RLTL QQKAKVKLDF VAPATGAHNY TLYFMSDAYM GCDQEYKFSV DVKEAETDSD SD

UniProtKB: U5 small nuclear ribonucleoprotein 200 kDa helicase

Macromolecule #10: 116 kDa U5 small nuclear ribonucleoprotein component

• Macromolecule: Name: 116 kDa U5 small nuclear ribonucleoprotein component / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 109.560625 KDa

Sequence

String:

MDTDLYDEFG NYIGPELDSD EDDDELGRET KDLDEMDDDD DDDDVGDHDD DHPGMEVVLH EDKKYYPTAE EVYGPEVETI VQEEDTQPL TEPIIKPVKT KKFTLMEQTL PVTVYEMDFL ADLMDNSELI RNVTLCGHLH HGKTCFVDCL IEQTHPEIRK R YDQDLCYT DILFTEQERG VGIKSTPVTV VLPDTKGKSY LFNIMDTPGH VNFSDEVTAG LRISDGVVLF IDAAEGVMLN TE RLIKHAV QERLAVTVCI NKIDRLILEL KLPPTDAYYK LRHIVDEVNG LISMYSTDEN LILSPLLGNV CFSSSQYSIC FTL GSFAKI YADTFGDINY QEFAKRLWGD IYFNPKTRKF TKKAPTSSSQ RSFVEFILEP LYKILAQVVG DVDTSLPRTL DELG IHLTK EELKLNIRPL LRLVCKKFFG EFTGFVDMCV QHIPSPKVGA KPKIEHTYTG GVDSDLGEAM SDCDPDGPLM CHTTK MYST DDGVQFHAFG RVLSGTIHAG QPVKVLGENY TLEDEEDSQI CTVGRLWISV ARYHIEVNRV PAGNWVLIEG VDQPIV KTA TITEPRGNEE AQIFRPLKFN TTSVIKIAVE PVNPSELPKM LDGLRKVNKS YPSLTTKVEE SGEHVILGTG ELYLDCV MH DLRKMYSEID IKVADPVVTF CETVVETSSL KCFAETPNKK NKITMIAEPL EKGLAEDIEN EVVQITWNRK KLGEFFQT K YDWDLLAARS IWAFGPDATG PNILVDDTLP SEVDKALLGS VKDSIVQGFQ WGTREGPLCD ELIRNVKFKI LDAVVAQEP LHRGGGQIIP TARRVVYSAF LMATPRLMEP YYFVEVQAPA DCVSAVYTVL ARRRGHVTQD APIPGSPLYT IKAFIPAIDS FGFETDLRT HTQGQAFSLS VFHHWQIVPG DPLDKSIVIR PLEPQPAPHL AREFMIKTRR RKGLSEDVSI SKFFDDPMLL E LAKQDVVL NYPM

UniProtKB: 116 kDa U5 small nuclear ribonucleoprotein component

Macromolecule #11: Peptidyl-prolyl cis-trans isomerase-like 3

• Macromolecule: Name: Peptidyl-prolyl cis-trans isomerase-like 3 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO / EC number: peptidylprolyl isomerase
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 18.177547 KDa

Sequence

String:

MSVTLHTDVG DIKIEVFCER TPKTCENFLA LCASNYYNGC IFHRNIKGFM VQTGDPTGTG RGGNSIWGKK FEDEYSEYLK HNVRGVVSM ANNGPNTNGS QFFITYGKQP HLDMKYTVFG KVIDGLETLD ELEKLPVNEK TYRPLNDVHI KDITIHANPF A Q

UniProtKB: Peptidyl-prolyl cis-trans isomerase-like 3

Macromolecule #12: Probable ATP-dependent RNA helicase DDX41

• Macromolecule: Name: Probable ATP-dependent RNA helicase DDX41 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 69.943672 KDa

Sequence

String:

MEESEPERKR ARTDEVPAGG SRSEAEDEDD EDYVPYVPLR QRRQLLLQKL LQRRRKGAAE EEQQDSGSEP RGDEDDIPLG PQSNVSLLD QHQHLKEKAE ARKESAKEKQ LKEEEKILES VAEGRALMSV KEMAKGITYD DPIKTSWTPP RYVLSMSEER H ERVRKKYH ILVEGDGIPP PIKSFKEMKF PAAILRGLKK KGIHHPTPIQ IQGIPTILSG RDMIGIAFTG SGKTLVFTLP VI MFCLEQE KRLPFSKREG PYGLIICPSR ELARQTHGIL EYYCRLLQED SSPLLRCALC IGGMSVKEQM ETIRHGVHMM VAT PGRLMD LLQKKMVSLD ICRYLALDEA DRMIDMGFEG DIRTIFSYFK GQRQTLLFSA TMPKKIQNFA KSALVKPVTI NVGR AGAAS LDVIQEVEYV KEEAKMVYLL ECLQKTPPPV LIFAEKKADV DAIHEYLLLK GVEAVAIHGG KDQEERTKAI EAFRE GKKD VLVATDVASK GLDFPAIQHV INYDMPEEIE NYVHRIGRTG RSGNTGIATT FINKACDESV LMDLKALLLE AKQKVP PVL QVLHCGDESM LDIGGERGCA FCGGLGHRIT DCPKLEAMQT KQVSNIGRKD YLAHSSMDF

UniProtKB: Probable ATP-dependent RNA helicase DDX41

Macromolecule #13: Peptidyl-prolyl cis-trans isomerase E

• Macromolecule: Name: Peptidyl-prolyl cis-trans isomerase E / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO / EC number: peptidylprolyl isomerase
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 33.475773 KDa

Sequence

String:

MATTKRVLYV GGLAEEVDDK VLHAAFIPFG DITDIQIPLD YETEKHRGFA FVEFELAEDA AAAIDNMNES ELFGRTIRVN LAKPMRIKE GSSRPVWSDD DWLKKFSGKT LEENKEEEGS EPPKAETQEG EPIAKKARSN PQVYMDIKIG NKPAGRIQML L RSDVVPMT AENFRCLCTH EKGFGFKGSS FHRIIPQFMC QGGDFTNHNG TGGKSIYGKK FDDENFILKH TGPGLLSMAN SG PNTNGSQ FFLTCDKTDW LDGKHVVFGE VTEGLDVLRQ IEAQGSKDGK PKQKVIIADC GEYV

UniProtKB: Peptidyl-prolyl cis-trans isomerase E

Macromolecule #14: Protein FAM50A

• Macromolecule: Name: Protein FAM50A / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 40.319695 KDa

Sequence

String:

MAQYKGAASE AGRAMHLMKK REKQREQMEQ MKQRIAEENI MKSNIDKKFS AHYDAVEAEL KSSTVGLVTL NDMKAKQEAL VKEREKQLA KKEQSKELQM KLEKLREKER KKEAKRKISS LSFTLEEEEE GGEEEEEAAM YEEEMEREEI TTKKRKLGKN P DVDTSFLP DRDREEEENR LREELRQEWE AKQEKIKSEE IEITFSYWDG SGHRRTVKMR KGNTMQQFLQ KALEILRKDF SE LRSAGVE QLMYIKEDLI IPHHHSFYDF IVTKARGKSG PLFNFDVHDD VRLLSDATVE KDESHAGKVV LRSWYEKNKH IFP ASRWEP YDPEKKWDKY TIR

UniProtKB: Protein FAM50A

Macromolecule #15: Pre-mRNA-splicing factor ISY1 homolog

• Macromolecule: Name: Pre-mRNA-splicing factor ISY1 homolog / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 33.046254 KDa

Sequence

String:

MARNAEKAMT ALARFRQAQL EEGKVKERRP FLASECTELP KAEKWRRQII GEISKKVAQI QNAGLGEFRI RDLNDEINKL LREKGHWEV RIKELGGPDY GKVGPKMLDH EGKEVPGNRG YKYFGAAKDL PGVRELFEKE PLPPPRKTRA ELMKAIDFEY Y GYLDEDDG VIVPLEQEYE KKLRAELVEK WKAEREARLA RGEKEEEEEE EEEINIYAVT EEESDEEGSQ EKGGDDSQQK FI AHVPVPS QQEIEEALVR RKKMELLQKY ASETLQAQSE EARRLLGY

UniProtKB: Pre-mRNA-splicing factor ISY1 homolog

Macromolecule #16: Nitric oxide synthase-interacting protein

• Macromolecule: Name: Nitric oxide synthase-interacting protein / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO / EC number: RING-type E3 ubiquitin transferase
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 33.224062 KDa

Sequence

String:

MTRHGKNCTA GAVYTYHEKK KDTAASGYGT QNIRLSRDAV KDFDCCCLSL QPCHDPVVTP DGYLYEREAI LEYILHQKKE IARQMKAYE KQRGTRREEQ KELQRAASQD HVRGFLEKES AIVSRPLNPF TAKALSGTSP DDVQPGPSVG PPSKDKDKVL P SFWIPSLT PEAKATKLEK PSRTVTCPMS GKPLRMSDLT PVHFTPLDSS VDRVGLITRS ERYVCAVTRD SLSNATPCAV LR PSGAVVT LECVEKLIRK DMVDPVTGDK LTDRDIIVLQ RGGTGFAGSG VKLQAEKSRP VMQA

UniProtKB: Nitric oxide synthase-interacting protein

Macromolecule #17: Splicing factor C9orf78

• Macromolecule: Name: Splicing factor C9orf78 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 33.751109 KDa

Sequence

String:

MPVVRKIFRR RRGDSESEED EQDSEEVRLK LEETREVQNL RKRPNGVSAV ALLVGEKVQE ETTLVDDPFQ MKTGGMVDMK KLKERGKDK ISEEEDLHLG TSFSAETNRR DEDADMMKYI ETELKKRKGI VEHEEQKVKP KNAEDCLYEL PENIRVSSAK K TEEMLSNQ MLSGIPEVDL GIDAKIKNII STEDAKARLL AEQQNKKKDS ETSFVPTNMA VNYVQHNRFY HEELNAPIRR NK EEPKARP LRVGDTEKPE PERSPPNRKR PANEKATDDY HYEKFKKMNR RY

UniProtKB: Splicing factor C9orf78

Macromolecule #18: PRKR-interacting protein 1

• Macromolecule: Name: PRKR-interacting protein 1 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 21.040176 KDa

Sequence

String:

MASPAASSVR PPRPKKEPQT LVIPKNAAEE QKLKLERLMK NPDKAVPIPE KMSEWAPRPP PEFVRDVMGS SAGAGSGEFH VYRHLRRRE YQRQDYMDAM AEKQKLDAEF QKRLEKNKIA AEEQTAKRRK KRQKLKEKKL LAKKMKLEQK KQEGPGQPKE Q GSSSSAEA SGTEEEEEVP SFTMGR

UniProtKB: PRKR-interacting protein 1

Macromolecule #20: Splicing factor Cactin

• Macromolecule: Name: Splicing factor Cactin / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 88.860062 KDa

Sequence

String:

MGRDTRSRSR SAGRRGRRRQ SQSGSRSRSR SHGRRNRRRR EDEGRRRRRR RSRERRSDSE EERWQRSGMR SRSPPRPKWH SRDGSSQSD SGEEQSRGQW ARRRRRARSW SPSSSASSSA SPGRSQSPRA AAAALSQQQS LQERLRLREE RKQQEELMKA F ETPEEKRA RRLAKKEAKE RKKREKMGWG EEYMGYTNTD NPFGDNNLLG TFIWNKALEK KGISHLEEKE LKERNKRIQE DN RLELQKV KQLRLERERE KAMREQELEM LQREKEAEHF KTWEEQEDNF HLQQAKLRSK IRIRDGRAKP IDLLAKYISA EDD DLAVEM HEPYTFLNGL TVADMEDLLE DIQVYMELEQ GKNADFWRDM TTITEDEISK LRKLEASGKG PGERREGVNA SVSS DVQSV FKGKTYNQLQ VIFQGIEGKI RAGGPNLDMG YWESLLQQLR AHMARARLRE RHQDVLRQKL YKLKQEQGVE SEPLF PILK QEPQSPSRSL EPEDAAPTPP GPSSEGGPAE AEVDGATPTE GDGDGDGEGE GEGEAVLMEE DLIQQSLDDY DAGRYS PRL LTAHELPLDA HVLEPDEDLQ RLQLSRQQLQ VTGDASESAE DIFFRRAKEG MGQDEAQFSV EMPLTGKAYL WADKYRP RK PRFFNRVHTG FEWNKYNQTH YDFDNPPPKI VQGYKFNIFY PDLIDKRSTP EYFLEACADN KDFAILRFHA GPPYEDIA F KIVNREWEYS HRHGFRCQFA NGIFQLWFHF KRYRYRR

UniProtKB: Splicing factor Cactin

Macromolecule #21: Protein FAM32A

• Macromolecule: Name: Protein FAM32A / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 13.213453 KDa

Sequence

String:

MEAYEQVQKG PLKLKGVAEL GVTKRKKKKK DKDKAKLLEA MGTSKKNEEE KRRGLDKRTP AQAAFEKMQE KRQMERILKK ASKTHKQRV EDFNRHLDTL TEHYDIPKVS WTK

UniProtKB: Protein FAM32A

Macromolecule #22: Pre-mRNA-splicing factor CWC22 homolog

• Macromolecule: Name: Pre-mRNA-splicing factor CWC22 homolog / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 57.929094 KDa

Sequence

String:

KNSLAYQRMS WEALKKSING LINKVNISNI SIIIQELLQE NIVRGRGLLS RSVLQAQSAS PIFTHVYAAL VAIINSKFPQ IGELILKRL ILNFRKGYRR NDKQLCLTAS KFVAHLINQN VAHEVLCLEM LTLLLERPTD DSVEVAIGFL KECGLKLTQV S PRGINAIF ERLRNILHES EIDKRVQYMI EVMFAVRKDG FKDHPIILEG LDLVEEDDQF THMLPLEDDY NPEDVLNVFK MD PNFMENE EKYKAIKKEI LDEGDTDSNT DQDAGSSEED EEEEEEEGEE DEEGQKVTIH DKTEINLVSF RRTIYLAIQS SLD FEECAH KLLKMEFPES QTKELCNMIL DCCAQQRTYE KFFGLLAGRF CMLKKEYMES FEGIFKEQYD TIHRLETNKL RNVA KMFAH LLYTDSLPWS VLECIKLSEE TTTSSSRIFV KIFFQELCEY MGLPKLNARL KDETLQPFFE GLLPRDNPRN TRFAI NFFT SIGLGGLTDE LREHLK

UniProtKB: Pre-mRNA-splicing factor CWC22 homolog

Macromolecule #24: Pleiotropic regulator 1

• Macromolecule: Name: Pleiotropic regulator 1 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 57.280758 KDa

Sequence

String:

MVEEVQKHSV HTLVFRSLKR THDMFVADNG KPVPLDEESH KRKMAIKLRN EYGPVLHMPT SKENLKEKGP QNATDSYVHK QYPANQGQE VEYFVAGTHP YPPGPGVALT ADTKIQRMPS ESAAQSLAVA LPLQTKADAN RTAPSGSEYR HPGASDRPQP T AMNSIVME TGNTKNSALM AKKAPTMPKP QWHPPWKLYR VISGHLGWVR CIAVEPGNQW FVTGSADRTI KIWDLASGKL KL SLTGHIS TVRGVIVSTR SPYLFSCGED KQVKCWDLEY NKVIRHYHGH LSAVYGLDLH PTIDVLVTCS RDSTARIWDV RTK ASVHTL SGHTNAVATV RCQAAEPQII TGSHDTTIRL WDLVAGKTRV TLTNHKKSVR AVVLHPRHYT FASGSPDNIK QWKF PDGSF IQNLSGHNAI INTLTVNSDG VLVSGADNGT MHLWDWRTGY NFQRVHAAVQ PGSLDSESGI FACAFDQSES RLLTA EADK TIKVYREDDT ATEETHPVSW KPEIIKRKRF

UniProtKB: Pleiotropic regulator 1

Macromolecule #25: SNW domain-containing protein 1

• Macromolecule: Name: SNW domain-containing protein 1 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 61.770648 KDa

Sequence

String:

MALTSFLPAP TQLSQDQLEA EEKARSQRSR QTSLVSSRRE PPPYGYRKGW IPRLLEDFGD GGAFPEIHVA QYPLDMGRKK KMSNALAIQ VDSEGKIKYD AIARQGQSKD KVIYSKYTDL VPKEVMNADD PDLQRPDEEA IKEITEKTRV ALEKSVSQKV A AAMPVRAA DKLAPAQYIR YTPSQQGVAF NSGAKQRVIR MVEMQKDPME PPRFKINKKI PRGPP(SEP)PPAP VMH(SEP) PSRKM TVKEQQEWKI PPCISNWKNA KGYTIPLDKR LAADGRGLQT VHINENFAKL AEALYIADRK AREAVEMRAQ VERKM AQKE KEKHEEKLRE MAQKARERRA GIKTHVEKED GEARERDEIR HDRRKERQHD RNLSRAAPDK RSKLQRNENR DISEVI ALG VPNPRTSNEV QYDQRLFNQS KGMDSGFAGG EDEIYNVYDQ AWRGGKDMAQ SIYRPSKNLD KDMYGDDLEA RIKTNRF VP DKEFSGSDRR QRGREGPVQF EEDPFGLDKF LEEAKQHGGS KRPSDSSRPK EHEHEGKKRR KE

UniProtKB: SNW domain-containing protein 1

Macromolecule #26: Protein BUD31 homolog

• Macromolecule: Name: Protein BUD31 homolog / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 17.03285 KDa

Sequence

String:

MPKVKRSRKA PPDGWELIEP TLDELDQKMR EAETEPHEGK RKVESLWPIF RIHHQKTRYI FDLFYKRKAI SRELYEYCIK EGYADKNLI AKWKKQGYEN LCCLRCIQTR DTNFGTNCIC RVPKSKLEVG RIIECTHCGC RGCSG

UniProtKB: Protein BUD31 homolog

Macromolecule #27: Pre-mRNA-splicing factor RBM22

• Macromolecule: Name: Pre-mRNA-splicing factor RBM22 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 46.959555 KDa

Sequence

String:

MATSLGSNTY NRQNWEDADF PILCQTCLGE NPYIRMTKEK YGKECKICAR PFTVFRWCPG VRMRFKKTEV CQTCSKLKNV CQTCLLDLE YGLPIQVRDA GLSFKDDMPK SDVNKEYYTQ NMEREISNSD GTRPVGMLGK ATSTSDMLLK LARTTPYYKR N RPHICSFW VKGECKRGEE CPYRHEKPTD PDDPLADQNI KDRYYGINDP VADKLLKRAS TMPRLDPPED KTITTLYVGG LG DTITETD LRNHFYQFGE IRTITVVQRQ QCAFIQFATR QAAEVAAEKS FNKLIVNGRR LNVKWGRSQA ARGKEKEKDG TTD SGIKLE PVPGLPGALP PPPAAEEEAS ANYFNLPPSG PPAVVNIALP PPPGIAPPPP PGFGPHMFHP MGPPPPFMRA PGPI HYPSQ DPQRMGAHAG KHSSP

UniProtKB: Pre-mRNA-splicing factor RBM22

Macromolecule #28: U5 small nuclear ribonucleoprotein 40 kDa protein

• Macromolecule: Name: U5 small nuclear ribonucleoprotein 40 kDa protein / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 39.359492 KDa

Sequence

String:

MIEQQKRKGP ELPLVPVKRQ RHELLLGAGS GPGAGQQQAT PGALLQAGPP RCSSLQAPIM LLSGHEGEVY CCKFHPNGST LASAGFDRL ILLWNVYGDC DNYATLKGHS GAVMELHYNT DGSMLFSAST DKTVAVWDSE TGERVKRLKG HTSFVNSCYP A RRGPQLVC TGSDDGTVKL WDIRKKAAIQ TFQNTYQVLA VTFNDTSDQI ISGGIDNDIK VWDLRQNKLT YTMRGHADSV TG LSLSSEG SYLLSNAMDN TVRVWDVRPF APKERCVKIF QGNVHNFEKN LLRCSWSPDG SKIAAGSADR FVYVWDTTSR RIL YKLPGH AGSINEVAFH PDEPIIISAS SDKRLYMGEI Q

UniProtKB: U5 small nuclear ribonucleoprotein 40 kDa protein

Macromolecule #29: Cell division cycle 5-like protein

• Macromolecule: Name: Cell division cycle 5-like protein / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 92.406883 KDa

Sequence

String:

MPRIMIKGGV WRNTEDEILK AAVMKYGKNQ WSRIASLLHR KSAKQCKARW YEWLDPSIKK TEWSREEEEK LLHLAKLMPT QWRTIAPII GRTAAQCLEH YEFLLDKAAQ RDNEEETTDD PRKLKPGEID PNPETKPARP DPIDMDEDEL EMLSEARARL A NTQGKKAK RKAREKQLEE ARRLAALQKR RELRAAGIEI QKKRKRKRGV DYNAEIPFEK KPALGFYDTS EENYQALDAD FR KLRQQDL DGELRSEKEG RDRKKDKQHL KRKKESDLPS AILQTSGVSE FTKKRSKLVL PAPQISDAEL QEVVKVGQAS EIA RQTAEE SGITNSASST LLSEYNVTNN SVALRTPRTP ASQDRILQEA QNLMALTNVD TPLKGGLNTP LHESDFSGVT PQRQ VVQTP NTVLSTPFRT PSNGAEGLTP RSGTTPKPVI NSTPGRTPLR DKLNINPEDG MADYSDPSYV KQMERESREH LRLGL LGLP APKNDFEIVL PENAEKELEE REIDDTYIED AADVDARKQA IRDAERVKEM KRMHKAVQKD LPRPSEVNET ILRPLN VEP PLTDLQKSEE LIKKEMITML HYDLLHHPYE PSGNKKGKTV GFGTNNSEHI TYLEHNPYEK FSKEELKKAQ DVLVQEM EV VKQGMSHGEL SSEAYNQVWE ECYSQVLYLP GQSRYTRANL ASKKDRIESL EKRLEINRGH MTTEAKRAAK MEKKMKIL L GGYQSRAMGL MKQLNDLWDQ IEQAHLELRT FEELKKHEDS AIPRRLECLK EDVQRQQERE KELQHRYADL LLEKETLKS KF

UniProtKB: Cell division cycle 5-like protein

Macromolecule #30: Spliceosome-associated protein CWC15 homolog

• Macromolecule: Name: Spliceosome-associated protein CWC15 homolog / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 26.674447 KDa

Sequence

String:

MTTAARPTFE PARGGRGKGE GDLSQLSKQY SSRDLPSHTK IKYRQTTQDA PEEVRNRDFR RELEERERAA AREKNRDRPT REHTTSSSV SKKPRLDQIP AANLDADDPL TDEEDEDFEE ESDDDDTAAL LAELEKIKKE RAEEQARKEQ EQKAEEERIR M ENILSGNP LLNLTGPSQP QANFKVKRRW DDDVVFKNCA KGVDDQKKDK RFVNDTLRSE FHKKFMEKYI K

UniProtKB: Spliceosome-associated protein CWC15 homolog

Macromolecule #31: Serine/arginine repetitive matrix protein 2

• Macromolecule: Name: Serine/arginine repetitive matrix protein 2 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 300.255312 KDa

Sequence

String:

MYNGIGLPTP RGSGTNGYVQ RNLSLVRGRR GERPDYKGEE ELRRLEAALV KRPNPDILDH ERKRRVELRC LELEEMMEEQ GYEEQQIQE KVATFRLMLL EKDVNPGGKE ETPGQRPAVT ETHQLAELNE KKNERLRAAF GISDSYVDGS SFDPQRRARE A KQPAPEPP KPYSLVRESS SSRSPTPKQK KKKKKKDRGR RSESSSPRRE RKKSSKKKKH RSESESKKRK HRSPTPKSKR KS KDKKRKR SRSTTPAPKS RRAHRSTSAD SASSSDTSRS RSRSAAAKTH TTALAGRSPS PASGRRGEGD APFSEPGTTS TQR PSSPET ATKQPSSPYE DKDKDKKEKS ATRPSPSPER SSTGPEPPAP TPLLAERHGG SPQPLATTPL SQEPVNPPSE ASPT RDRSP PKSPEKLPQS SSSESSPPSP QPTKVSRHAS SSPESPKPAP APGSHREISS SPTSKNRSHG RAKRDKSHSH TPSRR MGRS RSPATAKRGR SRSRTPTKRG HSRSRSPQWR RSRSAQRWGR SRSPQRRGRS RSPQRPGWSR SRNTQRRGRS RSARRG RSH SRSPATRGRS RSRTPARRGR SRSRTPARRR SRSRTPTRRR SRSRTPARRG RSRSRTPARR RSRTRSPVRR RSRSRSP AR RSGRSRSRTP ARRGRSRSRT PARRGRSRSR TPARRSGRSR SRTPARRGRS RSRTPRRGRS RSRSLVRRGR SHSRTPQR R GRSGSSSERK NKSRTSQRRS RSNSSPEMKK SRISSRRSRS LSSPRSKAKS RLSLRRSLSG SSPCPKQKSQ TPPRRSRSG SSQPKAKSRT PPRRSRSSSS PPPKQKSKTP SRQSHSSSSP HPKVKSGTPP RQGSITSPQA NEQSVTPQRR SCFESSPDPE LKSRTPSRH SCSGSSPPRV KSSTPPRQSP SRSSSPQPKV KAIISPRQRS HSGSSSPSPS RVTSRTTPRR SRSVSPCSNV E SRLLPRYS HSGSSSPDTK VKPETPPRQS HSGSISPYPK VKAQTPPGPS LSGSKSPCPQ EKSKDSLVQS CPGSLSLCAG VK SSTPPGE SYFGVSSLQL KGQSQTSPDH RSDTSSPEVR QSHSESPSLQ SKSQTSPKGG RSRSSSPVTE LASRSPIRQD RGE FSASPM LKSGMSPEQS RFQSDSSSYP TVDSNSLLGQ SRLETAESKE KMALPPQEDA TASPPRQKDK FSPFPVQDRP ESSL VFKDT LRTPPRERSG AGSSPETKEQ NSALPTSSQD EELMEVVEKS EEPAGQILSH LSSELKEMST SNFESSPEVE ERPAV SLTL DQSQSQASLE AVEVPSMASS WGGPHFSPEH KELSNSPLRE NSFGSPLEFR NSGPLGTEMN TGFSSEVKED LNGPFL NQL ETDPSLDMKE QSTRSSGHSS SELSPDAVEK AGMSSNQSIS SPVLDAVPRT PSRERSSSAS SPEMKDGLPR TPSRRSR SG SSPGLRDGSG TPSRHSLSGS SPGMKDIPRT PSRGRSECDS SPEPKALPQT PRPRSRSPSS PELNNKCLTP QRERSGSE S SVDQKTVART PLGQRSRSGS SQELDVKPSA SPQERSESDS SPDSKAKTRT PLRQRSRSGS SPEVDSKSRL SPRRSRSGS SPEVKDKPRA APRAQSGSDS SPEPKAPAPR ALPRRSRSGS SSKGRGPSPE GSSSTESSPE HPPKSRTARR GSRSSPEPKT KSRTPPRRR SSRSSPELTR KARLSRRSRS ASSSPETRSR TPPRHRRSPS VSSPEPAEKS RSSRRRRSAS SPRTKTTSRR G RSPSPKPR GLQRSRSRSR REKTRTTRRR DRSGSSQSTS RRRQRSRSRS RVTRRRRGGS GYHSRSPARQ ESSRTSSRRR RG RSRTPPT SRKRSRSRTS PAPWKRSRSR ASPATHRRSR SRTPLISRRR SRSRTSPVSR RRSRSRTSVT RRRSRSRASP VSR RRSRSR TPPVTRRRSR SRTPTTRRRS RSRTPPVTRR RSRSRTPPVT RRRSRSRTSP ITRRRSRSRT SPVTRRRSRS RTSP VTRRR SRSRTSPVTR RRSRSRTPPA IRRRSRSRTP LLPRKRSRSR SPLAIRRRSR SRTPRTARGK RSLTRSPPAI RRRSA SGSS SDRSRSATPP ATRNHSGSRT PPVALNSSRM SCFSRPSMSP TPLDRCRSPG MLEPLGSSRT PMSVLQQAGG SMMDGP GPR IPDHQRTSVP ENHAQSRIAL ALTAISLGTA RPPPSMSAAG LAARMSQVPA PVPLMSLRTA PAANLASRIP AASAAAM NL ASARTPAIPT AVNLADSRTP AAAAAMNLAS PRTAVAPSAV NLADPRTPTA PAVNLAGART PAALAALSLT GSGTPPTA A NYPSSSRTPQ APASANLVGP RSAHATAPVN IAGSRTAAAL APASLTSARM APALSGANLT SPRVPLSAYE RVSGRTSPP LLDRARSRTP PSAPSQSRMT SERAPSPSSR MGQAPSQSLL PPAQDQPRSP VPSAFSDQSR CLIAQTTPVA GSQSLSSGAV ATTTSSAGD HNGMLSVPAP GVPHSDVGEP PASTGAQQPS ALAALQPAKE RRSSSSSSSS SSSSSSSSSS SSSSSSSGSS S SDSEGSSL PVQPEVALKR VPSPTPAPKE AVREGRPPEP TPAKRKRRSS SSSSSSSSSS SSSSSSSSSS SSSSSSSSSS SS SSSSSSS SPSPAKPGPQ ALPKPASPKK PPPGERRSRS PRKPIDSLRD SRSLSYSPVE RRRPSPQPSP RDQQSSSSER GSR RGQRGD SRSPSHKRRR ETPSPRPMRH RSSRSP

UniProtKB: Serine/arginine repetitive matrix protein 2

Macromolecule #32: Crooked neck-like protein 1

• Macromolecule: Name: Crooked neck-like protein 1 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 83.281492 KDa

Sequence

String:

MAASTAAGKQ RIPKVAKVKN KAPAEVQITA EQLLREAKER ELELLPPPPQ QKITDEEELN DYKLRKRKTF EDNIRKNRTV ISNWIKYAQ WEESLKEIQR ARSIYERALD VDYRNITLWL KYAEMEMKNR QVNHARNIWD RAITTLPRVN QFWYKYTYME E MLGNVAGA RQVFERWMEW QPEEQAWHSY INFELRYKEV DRARTIYERF VLVHPDVKNW IKYARFEEKH AYFAHARKVY ER AVEFFGD EHMDEHLYVA FAKFEENQKE FERVRVIYKY ALDRISKQDA QELFKNYTIF EKKFGDRRGI EDIIVSKRRF QYE EEVKAN PHNYDAWFDY LRLVESDAEA EAVREVYERA IANVPPIQEK RHWKRYIYLW INYALYEELE AKDPERTRQV YQAS LELIP HKKFTFAKMW ILYAQFEIRQ KNLSLARRAL GTSIGKCPKN KLFKVYIELE LQLREFDRCR KLYEKFLEFG PENCT SWIK FAELETILGD IDRARAIYEL AISQPRLDMP EVLWKSYIDF EIEQEETERT RNLYRRLLQR TQHVKVWISF AQFELS SGK EGSLTKCRQI YEEANKTMRN CEEKEERLML LESWRSFEEE FGTASDKERV DKLMPEKVKK RRKVQTDDGS DAGWEEY FD YIFPEDAANQ PNLKLLAMAK LWKKQQQEKE DAEHHPDEDV DESES

UniProtKB: Crooked neck-like protein 1

Macromolecule #33: Pre-mRNA-splicing factor SYF1

• Macromolecule: Name: Pre-mRNA-splicing factor SYF1 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 100.148711 KDa

Sequence

String:

MVVMARLSRP ERPDLVFEEE DLPYEEEIMR NQFSVKCWLR YIEFKQGAPK PRLNQLYERA LKLLPCSYKL WYRYLKARRA QVKHRCVTD PAYEDVNNCH ERAFVFMHKM PRLWLDYCQF LMDQGRVTHT RRTFDRALRA LPITQHSRIW PLYLRFLRSH P LPETAVRG YRRFLKLSPE SAEEYIEYLK SSDRLDEAAQ RLATVVNDER FVSKAGKSNY QLWHELCDLI SQNPDKVQSL NV DAIIRGG LTRFTDQLGK LWCSLADYYI RSGHFEKARD VYEEAIRTVM TVRDFTQVFD SYAQFEESMI AAKMETASEL GRE EEDDVD LELRLARFEQ LISRRPLLLN SVLLRQNPHH VHEWHKRVAL HQGRPREIIN TYTEAVQTVD PFKATGKPHT LWVA FAKFY EDNGQLDDAR VILEKATKVN FKQVDDLASV WCQCGELELR HENYDEALRL LRKATALPAR RAEYFDGSEP VQNRV YKSL KVWSMLADLE ESLGTFQSTK AVYDRILDLR IATPQIVINY AMFLEEHKYF EESFKAYERG ISLFKWPNVS DIWSTY LTK FIARYGGRKL ERARDLFEQA LDGCPPKYAK TLYLLYAQLE EEWGLARHAM AVYERATRAV EPAQQYDMFN IYIKRAA EI YGVTHTRGIY QKAIEVLSDE HAREMCLRFA DMECKLGEID RARAIYSFCS QICDPRTTGA FWQTWKDFEV RHGNEDTI K EMLRIRRSVQ ATYNTQVNFM ASQMLKVSGS ATGTVSDLAP GQSGMDDMKL LEQRAEQLAA EAERDQPLRA QSKILFVRS DASREELAEL AQQVNPEEIQ LGEDEDEDEM DLEPNEVRLE QQSVPAAVFG SLKED

UniProtKB: Pre-mRNA-splicing factor SYF1

Macromolecule #34: Intron-binding protein aquarius

• Macromolecule: Name: Intron-binding protein aquarius / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 171.502453 KDa

Sequence

String:

MAAPAQPKKI VAPTVSQINA EFVTQLACKY WAPHIKKKSP FDIKVIEDIY EKEIVKSRFA IRKIMLLEFS QYLENYLWMN YSPEVSSKA YLMSICCMVN EKFRENVPAW EIFKKKPDHF PFFFKHILKA ALAETDGEFS LHEQTVLLLF LDHCFNSLEV D LIRSQVQQ LISLPMWMGL QLARLELELK KTPKLRKFWN LIKKNDEKMD PEAREQAYQE RRFLSQLIQK FISVLKSVPL SE PVTMDKV HYCERFIELM IDLEALLPTR RWFNTILDDS HLLVHCYLSN LVRREEDGHL FSQLLDMLKF YTGFEINDQT GNA LTENEM TTIHYDRITS LQRAAFAHFP ELYDFALSNV AEVDTRESLV KFFGPLSSNT LHQVASYLCL LPTLPKNEDT TFDK EFLLE LLVSRHERRI SQIQQLNQMP LYPTEKIIWD ENIVPTEYYS GEGCLALPKL NLQFLTLHDY LLRNFNLFRL ESTYE IRQD IEDSVSRMKP WQSEYGGVVF GGWARMAQPI VAFTVVEVAK PNIGENWPTR VRADVTINLN VRDHIKDEWE GLRKHD VCF LITVRPTKPY GTKFDRRRPF IEQVGLVYVR GCEIQGMLDD KGRVIEDGPE PRPNLRGESR TFRVFLDPNQ YQQDMTN TI QNGAEDVYET FNIIMRRKPK ENNFKAVLET IRNLMNTDCV VPDWLHDIIL GYGDPSSAHY SKMPNQIATL DFNDTFLS I EHLKASFPGH NVKVTVEDPA LQIPPFRITF PVRSGKGKKR KDADVEDEDT EEAKTLIVEP HVIPNRGPYP YNQPKRNTI QFTHTQIEAI RAGMQPGLTM VVGPPGTGKT DVAVQIISNI YHNFPEQRTL IVTHSNQALN QLFEKIMALD IDERHLLRLG HGEEELETE KDFSRYGRVN YVLARRIELL EEVKRLQKSL GVPGDASYTC ETAGYFFLYQ VMSRWEEYIS KVKNKGSTLP D VTEVSTFF PFHEYFANAP QPIFKGRSYE EDMEIAEGCF RHIKKIFTQL EEFRASELLR SGLDRSKYLL VKEAKIIAMT CT HAALKRH DLVKLGFKYD NILMEEAAQI LEIETFIPLL LQNPQDGFSR LKRWIMIGDH HQLPPVIKNM AFQKYSNMEQ SLF TRFVRV GVPTVDLDAQ GRARASLCNL YNWRYKNLGN LPHVQLLPEF STANAGLLYD FQLINVEDFQ GVGESEPNPY FYQN LGEAE YVVALFMYMC LLGYPADKIS ILTTYNGQKH LIRDIINRRC GNNPLIGRPN KVTTVDRFQG QQNDYILLSL VRTRA VGHL RDVRRLVVAM SRARLGLYIF ARVSLFQNCF ELTPAFSQLT ARPLHLHIIP TEPFPTTRKN GERPSHEVQI IKNMPQ MAN FVYNMYMHLI QTTHHYHQTL LQLPPAMVEE GEEVQNQETE LETEEEAMTV QADIIPSPTD TSCRQETPAF QTDTTPS ET GATSTPEAIP ALSETTPTVV GAVSAPAEAN TPQDATSAPE ETK

UniProtKB: RNA helicase aquarius

Macromolecule #35: ATP-dependent RNA helicase DHX8

• Macromolecule: Name: ATP-dependent RNA helicase DHX8 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 139.522312 KDa

Sequence

String:

MAVAVAMAGA LIGSEPGPAE ELAKLEYLSL VSKVCTELDN HLGINDKDLA EFVISLAEKN TTFDTFKASL VKNGAEFTDS LISNLLRLI QTMRPPAKPS TSKDPVVKPK TEKEKLKELF PVLCQPDNPS VRTMLDEDDV KVAVDVLKEL EALMPSAAGQ E KQRDAEHR DRTKKKKRSR SRDRNRDRDR DRERNRDRDH KRRHRSRSRS RSRTRERNKV KSRYRSRSRS QSPPKDRKDR DK YGERNLD RWRDKHVDRP PPEEPTIGDI YNGKVTSIMQ FGCFVQLEGL RKRWEGLVHI SELRREGRVA NVADVVSKGQ RVK VKVLSF TGTKTSLSMK DVDQETGEDL NPNRRRNLVG ETNEETSMRN PDRPTHLSLV SAPEVEDDSL ERKRLTRISD PEKW EIKQM IAANVLSKEE FPDFDEETGI LPKVDDEEDE DLEIELVEEE PPFLRGHTKQ SMDMSPIKIV KNPDGSLSQA AMMQS ALAK ERRELKQAQR EAEMDSIPMG LNKHWVDPLP DAEGRQIAAN MRGIGMMPND IPEWKKHAFG GNKASYGKKT QMSILE QRE SLPIYKLKEQ LVQAVHDNQI LIVIGETGSG KTTQITQYLA EAGYTSRGKI GCTQPRRVAA MSVAKRVSEE FGCCLGQ EV GYTIRFEDCT SPETVIKYMT DGMLLRECLI DPDLTQYAII MLDEAHERTI HTDVLFGLLK KTVQKRQDMK LIVTSATL D AVKFSQYFYE APIFTIPGRT YPVEILYTKE PETDYLDASL ITVMQIHLTE PPGDILVFLT GQEEIDTACE ILYERMKSL GPDVPELIIL PVYSALPSEM QTRIFDPAPP GSRKVVIATN IAETSLTIDG IYYVVDPGFV KQKVYNSKTG IDQLVVTPIS QAQAKQRAG RAGRTGPGKC YRLYTERAYR DEMLTTNVPE IQRTNLASTV LSLKAMGIND LLSFDFMDAP PMETLITAME Q LYTLGALD DEGLLTRLGR RMAEFPLEPM LCKMLIMSVH LGCSEEMLTI VSMLSVQNVF YRPKDKQALA DQKKAKFHQT EG DHLTLLA VYNSWKNNKF SNPWCYENFI QARSLRRAQD IRKQMLGIMD RHKLDVVSCG KSTVRVQKAI CSGFFRNAAK KDP QEGYRT LIDQQVVYIH PSSALFNRQP EWVVYHELVL TTKEYMREVT TIDPRWLVEF APAFFKVSDP TKLSKQKKQQ RLEP LYNRY EEPNAWRISR AFRRR

UniProtKB: ATP-dependent RNA helicase DHX8

Macromolecule #36: U2 small nuclear ribonucleoprotein A'

• Macromolecule: Name: U2 small nuclear ribonucleoprotein A' / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 28.456584 KDa

Sequence

String:

MVKLTAELIE QAAQYTNAVR DRELDLRGYK IPVIENLGAT LDQFDAIDFS DNEIRKLDGF PLLRRLKTLL VNNNRICRIG EGLDQALPC LTELILTNNS LVELGDLDPL ASLKSLTYLS ILRNPVTNKK HYRLYVIYKV PQVRVLDFQK VKLKERQEAE K MFKGKRGA QLAKDIARRS KTFNPGAGLP TDKKKGGPSP GDVEAIKNAI ANASTLAEVE RLKGLLQSGQ IPGRERRSGP TD DGEEEME EDTVTNGS

UniProtKB: U2 small nuclear ribonucleoprotein A'

Macromolecule #37: U2 small nuclear ribonucleoprotein B''

• Macromolecule: Name: U2 small nuclear ribonucleoprotein B'' / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 25.524367 KDa

Sequence

String:

MDIRPNHTIY INNMNDKIKK EELKRSLYAL FSQFGHVVDI VALKTMKMRG QAFVIFKELG SSTNALRQLQ GFPFYGKPMR IQYAKTDSD IISKMRGTFA DKEKKKEKKK AKTVEQTATT TNKKPGQGTP NSANTQGNST PNPQVPDYPP NYILFLNNLP E ETNEMMLS MLFNQFPGFK EVRLVPGRHD IAFVEFENDG QAGAARDALQ GFKITPSHAM KITYAKK

UniProtKB: U2 small nuclear ribonucleoprotein B''

Macromolecule #38: NF-kappa-B-activating protein

• Macromolecule: Name: NF-kappa-B-activating protein / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 47.25266 KDa

Sequence

String:

MAPVSGSRSP DREASGSGGR RRSSSKSPKP SKSARSPRGR RSRSHSCSRS GDRNGLTHQL GGLSQGSRNQ SYRSRSRSRS RERPSAPRG IPFASASSSV YYGSYSRPYG SDKPWPSLLD KEREESLRQK RLSERERIGE LGAPEVWGLS PKNPEPDSDE H TPVEDEEP KKSTTSASTS EEEKKKKSSR SKERSKKRRK KKSSKRKHKK YSEDSDSDSD SETDSSDEDN KRRAKKAKKK EK KKKHRSK KYKKKRSKKS RKESSDSSSK ESQEEFLENP WKDRTKAEEP SDLIGPEAPK TLTSQDDKPL NYGHALLPGE GAA MAEYVK AGKRIPRRGE IGLTSEEIAS FECSGYVMSG SRHRRMEAVR LRKENQIYSA DEKRALASFN QEERRKRENK ILAS FREMV YRKTKGKDDK

UniProtKB: NF-kappa-B-activating protein

Macromolecule #39: Small nuclear ribonucleoprotein Sm D2

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D2 / type: protein_or_peptide / ID: 39 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 13.551928 KDa

Sequence

String:

MSLLNKPKSE MTPEELQKRE EEEFNTGPLS VLTQSVKNNT QVLINCRNNK KLLGRVKAFD RHCNMVLENV KEMWTEVPKS GKGKKKSKP VNKDRYISKM FLRGDSVIVV LRNPLIAGK

UniProtKB: Small nuclear ribonucleoprotein Sm D2

Macromolecule #40: Small nuclear ribonucleoprotein-associated proteins B and B'

• Macromolecule: Name: Small nuclear ribonucleoprotein-associated proteins B and B'; type: protein_or_peptide / ID: 40 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 24.642131 KDa

Sequence

String:

MTVGKSSKML QHIDYRMRCI LQDGRIFIGT FKAFDKHMNL ILCDCDEFRK IKPKNSKQAE REEKRVLGLV LLRGENLVSM TVEGPPPKD TGIARVPLAG AAGGPGIGRA AGRGIPAGVP MPQAPAGLAG PVRGVGGPSQ QVMTPQGRGT VAAAAAAATA S IAGAPTQY PPGRGGPPPP MGRGAPPPGM MGPPPGMRPP MGPPMGIPPG RGTPMGMPPP GMRPPPPGMR GPPPPGMRPP RP

UniProtKB: Small nuclear ribonucleoprotein-associated proteins B and B'

Macromolecule #41: Small nuclear ribonucleoprotein Sm D1

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D1 / type: protein_or_peptide / ID: 41 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 13.310653 KDa

Sequence

String:

MKLVRFLMKL SHETVTIELK NGTQVHGTIT GVDVSMNTHL KAVKMTLKNR EPVQLETLSI RGNNIRYFIL PDSLPLDTLL VDVEPKVKS KKREAVAGRG RGRGRGRGRG RGRGRGGPRR

UniProtKB: Small nuclear ribonucleoprotein Sm D1

Macromolecule #42: Small nuclear ribonucleoprotein Sm D3

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D3 / type: protein_or_peptide / ID: 42 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 13.940308 KDa

Sequence

String:

MSIGVPIKVL HEAEGHIVTC ETNTGEVYRG KLIEAEDNMN CQMSNITVTY RDGRVAQLEQ VYIRGSKIRF LILPDMLKNA PMLKSMKNK NQGSGAGRGK AAILKAQVAA RGRGRGMGRG NIFQKRR

UniProtKB: Small nuclear ribonucleoprotein Sm D3

Macromolecule #43: Small nuclear ribonucleoprotein E

• Macromolecule: Name: Small nuclear ribonucleoprotein E / type: protein_or_peptide / ID: 43 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 10.817601 KDa

Sequence

String:

MAYRGQGQKV QKVMVQPINL IFRYLQNRSR IQVWLYEQVN MRIEGCIIGF DEYMNLVLDD AEEIHSKTKS RKQLGRIMLK GDNITLLQS VSN

UniProtKB: Small nuclear ribonucleoprotein E

Macromolecule #44: Small nuclear ribonucleoprotein F

• Macromolecule: Name: Small nuclear ribonucleoprotein F / type: protein_or_peptide / ID: 44 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 9.734171 KDa

Sequence

String:

MSLPLNPKPF LNGLTGKPVM VKLKWGMEYK GYLVSVDGYM NMQLANTEEY IDGALSGHLG EVLIRCNNVL YIRGVEEEEE DGEMRE

UniProtKB: Small nuclear ribonucleoprotein F

Macromolecule #45: Small nuclear ribonucleoprotein G

• Macromolecule: Name: Small nuclear ribonucleoprotein G / type: protein_or_peptide / ID: 45 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 8.508084 KDa

Sequence

String:

MSKAHPPELK KFMDKKLSLK LNGGRHVQGI LRGFDPFMNL VIDECVEMAT SGQQNNIGMV VIRGNSIIML EALERV

UniProtKB: Small nuclear ribonucleoprotein G

Macromolecule #46: Pre-mRNA-splicing factor SLU7

• Macromolecule: Name: Pre-mRNA-splicing factor SLU7 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 68.510844 KDa

Sequence

String:

MSATVVDAVN AAPLSGSKEM SLEEPKKMTR EDWRKKKELE EQRKLGNAPA EVDEEGKDIN PHIPQYISSV PWYIDPSKRP TLKHQRPQP EKQKQFSSSG EWYKRGVKEN SIITKYRKGA CENCGAMTHK KKDCFERPRR VGAKFTGTNI APDEHVQPQL M FDYDGKRD RWNGYNPEEH MKIVEEYAKV DLAKRTLKAQ KLQEELASGK LVEQANSPKH QWGEEEPNSQ MEKDHNSEDE DE DKYADDI DMPGQNFDSK RRITVRNLRI REDIAKYLRN LDPNSAYYDP KTRAMRENPY ANAGKNPDEV SYAGDNFVRY TGD TISMAQ TQLFAWEAYD KGSEVHLQAD PTKLELLYKS FKVKKEDFKE QQKESILEKY GGQEHLDAPP AELLLAQTED YVEY SRHGT VIKGQERAVA CSKYEEDVKI HNHTHIWGSY WKEGRWGYKC CHSFFKYSYC TGEAGKEIVN SEECIINEIT GEESV KKPQ TLMELHQEKL KEEKKKKKKK KKKHRKSSSD SDDEEKKHEK LKKALNAEEA RLLHVKETMQ IDERKRPYNS MYETRE PTE EEMEAYRMKR QRPDDPMASF LGQ

UniProtKB: Pre-mRNA-splicing factor SLU7

Macromolecule #47: Peptidyl-prolyl cis-trans isomerase-like 1

• Macromolecule: Name: Peptidyl-prolyl cis-trans isomerase-like 1 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO / EC number: peptidylprolyl isomerase
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 18.257805 KDa

Sequence

String:

MAAIPPDSWQ PPNVYLETSM GIIVLELYWK HAPKTCKNFA ELARRGYYNG TKFHRIIKDF MIQGGDPTGT GRGGASIYGK QFEDELHPD LKFTGAGILA MANAGPDTNG SQFFVTLAPT QWLDGKHTIF GRVCQGIGMV NRVGMVETNS QDRPVDDVKI I KAYPSG

UniProtKB: Peptidyl-prolyl cis-trans isomerase-like 1

Macromolecule #48: STING ER exit protein

• Macromolecule: Name: STING ER exit protein / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 25.66968 KDa

Sequence

String:

MPKVVSRSVV CSDTRDREEY DDGEKPLHVY YCLCGQMVLV LDCQLEKLPM RPRDRSRVID AAKHAHKFCN TEDEETMYLR RPEGIERQY RKKCAKCGLP LFYQSQPKNA PVTFIVDGAV VKFGQGFGKT NIYTQKQEPP KKVMMTKRTK DMGKFSSVTV S TIDEEEEE IEAREVADSY AQNAKVIEKQ LERKGMSKRR LQELAELEAK KAKMKGTLID NQFK

UniProtKB: STING ER exit protein

Macromolecule #49: Pre-mRNA-processing factor 17

• Macromolecule: Name: Pre-mRNA-processing factor 17 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 65.61218 KDa

Sequence

String:

MSAAIAALAA SYGSGSGSES DSDSESSRCP LPAADSLMHL TKSPSSKPSL AVAVDSAPEV AVKEDLETGV HLDPAVKEVQ YNPTYETMF APEFGPENPF RTQQMAAPRN MLSGYAEPAH INDFMFEQQR RTFATYGYAL DPSLDNHQVS AKYIGSVEEA E KNQGLTVF ETGQKKTEKR KKFKENDASN IDGFLGPWAK YVDEKDVAKP SEEEQKELDE ITAKRQKKGK QEEEKPGEEK TI LHVKEMY DYQGRSYLHI PQDVGVNLRS TMPPEKCYLP KKQIHVWSGH TKGVSAVRLF PLSGHLLLSC SMDCKIKLWE VYG ERRCLR TFIGHSKAVR DICFNTAGTQ FLSAAYDRYL KLWDTETGQC ISRFTNRKVP YCVKFNPDED KQNLFVAGMS DKKI VQWDI RSGEIVQEYD RHLGAVNTIV FVDENRRFVS TSDDKSLRVW EWDIPVDFKY IAEPSMHSMP AVTLSPNGKW LACQS MDNQ ILIFGAQNRF RLNKKKIFKG HMVAGYACQV DFSPDMSYVI SGDGNGKLNI WDWKTTKLYS RFKAHDKVCI GAVWHP HET SKVITCGWDG LIKLWD

UniProtKB: Pre-mRNA-processing factor 17

Macromolecule #50: Pre-mRNA-splicing factor SPF27

• Macromolecule: Name: Pre-mRNA-splicing factor SPF27 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 26.16342 KDa

Sequence

String:

MAGTGLVAGE VVVDALPYFD QGYEAPGVRE AAAALVEEET RRYRPTKNYL SYLTAPDYSA FETDIMRNEF ERLAARQPIE LLSMKRYEL PAPSSGQKND ITAWQECVNN SMAQLEHQAV RIENLELMSQ HGCNAWKVYN ENLVHMIEHA QKELQKLRKH I QDLNWQRK NMQLTAGSKL REMESNWVSL VSKNYEIERT IVQLENEIYQ IKQQHGEANK ENIRQDF

UniProtKB: Pre-mRNA-splicing factor SPF27

Macromolecule #51: Pre-mRNA-processing factor 19

• Macromolecule: Name: Pre-mRNA-processing factor 19 / type: protein_or_peptide / ID: 51 / Number of copies: 4 / Enantiomer: LEVO / EC number: RING-type E3 ubiquitin transferase
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 55.245547 KDa

Sequence

String:

MSLICSISNE VPEHPCVSPV SNHVYERRLI EKYIAENGTD PINNQPLSEE QLIDIKVAHP IRPKPPSATS IPAILKALQD EWDAVMLHS FTLRQQLQTT RQELSHALYQ HDAACRVIAR LTKEVTAARE ALATLKPQAG LIVPQAVPSS QPSVVGAGEP M DLGELVGM TPEIIQKLQD KATVLTTERK KRGKTVPEEL VKPEELSKYR QVASHVGLHS ASIPGILALD LCPSDTNKIL TG GADKNVV VFDKSSEQIL ATLKGHTKKV TSVVFHPSQD LVFSASPDAT IRIWSVPNAS CVQVVRAHES AVTGLSLHAT GDY LLSSSD DQYWAFSDIQ TGRVLTKVTD ETSGCSLTCA QFHPDGLIFG TGTMDSQIKI WDLKERTNVA NFPGHSGPIT SIAF SENGY YLATAADDSS VKLWDLRKLK NFKTLQLDNN FEVKSLIFDQ SGTYLALGGT DVQIYICKQW TEILHFTEHS GLTTG VAFG HHAKFIASTG MDRSLKFYSL

UniProtKB: Pre-mRNA-processing factor 19

Macromolecule #52: Pre-mRNA-splicing factor SYF2

• Macromolecule: Name: Pre-mRNA-splicing factor SYF2 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 28.780518 KDa

Sequence

String:

MAAIAASEVL VDSAEEGSLA AAAELAAQKR EQRLRKFREL HLMRNEARKL NHQEVVEEDK RLKLPANWEA KKARLEWELK EEEKKKECA ARGEDYEKVK LLEISAEDAE RWERKKKRKN PDLGFSDYAA AQLRQYHRLT KQIKPDMETY ERLREKHGEE F FPTSNSLL HGTHVPSTEE IDRMVIDLEK QIEKRDKYSR RRPYNDDADI DYINERNAKF NKKAERFYGK YTAEIKQNLE RG TAV

UniProtKB: Pre-mRNA-splicing factor SYF2

Macromolecule #53: Splicing regulator SDE2

• Macromolecule: Name: Splicing regulator SDE2 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 49.818027 KDa

Sequence

String:

MAEAAALVWI RGPGFGCKAV RCASGRCTVR DFIHRHCQDQ NVPVENFFVK CNGALINTSD TVQHGAVYSL EPRLCGGKGG FGSMLRALG AQIEKTTNRE ACRDLSGRRL RDVNHEKAMA EWVKQQAERE AEKEQKRLER LQRKLVEPKH CFTSPDYQQQ C HEMAERLE DSVLKGMQAA SSKMVSAEIS ENRKRQWPTK SQTDRGASAG KRRCFWLGME GLETAEGSNS ESSDDDSEEA PS TSGMGFH APKIGSNGVE MAAKFPSGSQ RARVVNTDHG SPEQLQIPVT DSGRHILEDS CAELGESKEH MESRMVTETE ETQ EKKAES KEPIEEEPTG AGLNKDKETE ERTDGERVAE VAPEERENVA VAKLQESQPG NAVIDKETID LLAFTSVAEL ELLG LEKLK CELMALGLKC GGTLQERAAR LFSVRGLAKE QIDPALFAKP LKGKKK

UniProtKB: Splicing regulator SDE2

Macromolecule #54: POTASSIUM ION

• Macromolecule: Name: POTASSIUM ION / type: ligand / ID: 54 / Number of copies: 2 / Formula: K
• Molecular weight: Theoretical: 39.098 Da

Macromolecule #55: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 55 / Number of copies: 7 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #56: ADENOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 56 / Number of copies: 1 / Formula: ATP
• Molecular weight: Theoretical: 507.181 Da

Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

Macromolecule #57: INOSITOL HEXAKISPHOSPHATE

• Macromolecule: Name: INOSITOL HEXAKISPHOSPHATE / type: ligand / ID: 57 / Number of copies: 1 / Formula: IHP
• Molecular weight: Theoretical: 660.035 Da

Chemical component information

ChemComp-IHP:
INOSITOL HEXAKISPHOSPHATE / Phytic acid

Macromolecule #58: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 58 / Number of copies: 1 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

Macromolecule #59: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 59 / Number of copies: 6 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 0.8 mg/mL
• Buffer: pH: 7.8
• Grid: Model: Quantifoil R2/2 / Material: GOLD / Mesh: 200 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 100 sec.
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV
• Details: 4 microliter sample was applied to a grid. After 15 s, the grid was blotted for 3 s with a blot force of 5 and vitrified by plunging into liquid ethane using FEI Vitrobot Mark IV.

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: C2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 3.0 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 81000
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 1 / Number real images: 14388 / Average exposure time: 1.465 sec. / Average electron dose: 44.24 e/Ų
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 1643674
• Startup model: Type of model: INSILICO MODEL
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 2.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 1150057

Atomic model buiding 1

Initial model

PDB ID	Chain
6qdv	source_name: PDB, initial_model_type: experimental model
	source_name: AlphaFold, initial_model_type: in silico model
7os2	chain_id: B, source_name: PDB, initial_model_type: experimental model
7os2	chain_id: CT, source_name: PDB, initial_model_type: experimental model
4pj3	chain_id: U, source_name: PDB, initial_model_type: experimental model
5mqf	chain_id: I, source_name: PDB, initial_model_type: experimental model
6bk8	chain_id: I, source_name: PDB, initial_model_type: experimental model
3mdf	chain_id: CE, source_name: PDB, initial_model_type: experimental model
5hly	chain_id: CD, source_name: PDB, initial_model_type: experimental model
2ok3	chain_id: C3, source_name: PDB, initial_model_type: experimental model
6hyu	chain_id: V, source_name: PDB, initial_model_type: experimental model

• Refinement: Space: REAL / Protocol: FLEXIBLE FIT

Output model

PDB-8c6j:
Human spliceosomal PM5 C* complex