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Yorodumi- EMDB-16160: Cryo-EM structure of the Arabidopsis thaliana I+III2 supercomplex... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-16160 | |||||||||
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Title | Cryo-EM structure of the Arabidopsis thaliana I+III2 supercomplex (Conformation 2 membrane arm) | |||||||||
Map data | Focused map | |||||||||
Sample |
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Keywords | Plant / Mitochondria / Complex / MEMBRANE PROTEIN | |||||||||
Biological species | Arabidopsis thaliana (thale cress) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.61 Å | |||||||||
Authors | Klusch N / Kuehlbrandt W | |||||||||
Funding support | Germany, 1 items
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Citation | Journal: To Be Published Title: Cryo-EM structure of the respiratory I + III2 supercomplex from Arabidopsis thaliana at 2 A resolution Authors: Klusch N / Dreimann M / Senkler J / Rugen N / Kuehlbrandt W / Braun HP | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_16160.map.gz | 105.9 MB | EMDB map data format | |
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Header (meta data) | emd-16160-v30.xml emd-16160.xml | 13.6 KB 13.6 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_16160_fsc.xml | 26.6 KB | Display | FSC data file |
Images | emd_16160.png | 111.3 KB | ||
Filedesc metadata | emd-16160.cif.gz | 3.9 KB | ||
Others | emd_16160_half_map_1.map.gz emd_16160_half_map_2.map.gz | 1.3 GB 1.3 GB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-16160 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-16160 | HTTPS FTP |
-Validation report
Summary document | emd_16160_validation.pdf.gz | 951.3 KB | Display | EMDB validaton report |
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Full document | emd_16160_full_validation.pdf.gz | 950.9 KB | Display | |
Data in XML | emd_16160_validation.xml.gz | 33.9 KB | Display | |
Data in CIF | emd_16160_validation.cif.gz | 45.6 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-16160 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-16160 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_16160.map.gz / Format: CCP4 / Size: 1.6 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Annotation | Focused map | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.573 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: Focused half map
File | emd_16160_half_map_1.map | ||||||||||||
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Annotation | Focused half map | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Focused half map
File | emd_16160_half_map_2.map | ||||||||||||
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Annotation | Focused half map | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Mitochondrial Arabidopsis thaliana I+III2 supercomplex (Conformat...
Entire | Name: Mitochondrial Arabidopsis thaliana I+III2 supercomplex (Conformation 2 membrane arm) |
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Components |
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-Supramolecule #1: Mitochondrial Arabidopsis thaliana I+III2 supercomplex (Conformat...
Supramolecule | Name: Mitochondrial Arabidopsis thaliana I+III2 supercomplex (Conformation 2 membrane arm) type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Arabidopsis thaliana (thale cress) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 0.18 mg/mL |
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Buffer | pH: 7.4 |
Grid | Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 400 / Support film - #0 - Film type ID: 1 / Support film - #0 - Material: CARBON / Support film - #0 - topology: CONTINUOUS / Support film - #1 - Film type ID: 2 / Support film - #1 - Material: GRAPHENE / Support film - #1 - topology: CONTINUOUS / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 15 sec. |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 70 % / Chamber temperature: 283.15 K / Instrument: FEI VITROBOT MARK IV |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.5 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 215000 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |