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- EMDB-16163: Cryo-EM structure of the Arabidopsis thaliana I+III2 supercomplex... -
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Open data
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Basic information
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Title | Cryo-EM structure of the Arabidopsis thaliana I+III2 supercomplex (Conformation 2 CIII) | |||||||||
![]() | Focused map | |||||||||
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![]() | Plant / Mitochondria / Complex / MEMBRANE PROTEIN | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.77 Å | |||||||||
![]() | Klusch N / Kuehlbrandt W | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Cryo-EM structure of the respiratory I + III2 supercomplex from Arabidopsis thaliana at 2 A resolution Authors: Klusch N / Dreimann M / Senkler J / Rugen N / Kuehlbrandt W / Braun HP | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 90.7 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 13.6 KB 13.6 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 26.6 KB | Display | ![]() |
Images | ![]() | 138.2 KB | ||
Filedesc metadata | ![]() | 3.9 KB | ||
Others | ![]() ![]() | 1.3 GB 1.3 GB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 920.8 KB | Display | ![]() |
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Full document | ![]() | 920.4 KB | Display | |
Data in XML | ![]() | 33.8 KB | Display | |
Data in CIF | ![]() | 45.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
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Annotation | Focused map | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.573 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: Focused half map
File | emd_16163_half_map_1.map | ||||||||||||
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Annotation | Focused half map | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Focused half map
File | emd_16163_half_map_2.map | ||||||||||||
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Annotation | Focused half map | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Mitochondrial Arabidopsis thaliana I+III2 supercomplex (Conformat...
Entire | Name: Mitochondrial Arabidopsis thaliana I+III2 supercomplex (Conformation 2 CIII). |
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Components |
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-Supramolecule #1: Mitochondrial Arabidopsis thaliana I+III2 supercomplex (Conformat...
Supramolecule | Name: Mitochondrial Arabidopsis thaliana I+III2 supercomplex (Conformation 2 CIII). type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: ![]() ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 0.18 mg/mL |
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Buffer | pH: 7.4 |
Grid | Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 400 / Support film - #0 - Film type ID: 1 / Support film - #0 - Material: CARBON / Support film - #0 - topology: CONTINUOUS / Support film - #1 - Film type ID: 2 / Support film - #1 - Material: GRAPHENE / Support film - #1 - topology: CONTINUOUS / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 15 sec. |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 70 % / Chamber temperature: 283.15 K / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.5 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 215000 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |