+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-16155 | ||||||||||||||||||||||||
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タイトル | Structure of rabbit 80S ribosome translating beta-tubulin in complex with tetratricopeptide protein 5 (TTC5) and S-phase Cyclin A Associated Protein residing in the ER (SCAPER) | ||||||||||||||||||||||||
マップデータ | Sharpened map of the 60S subunit bound to SCAPER and TTC5 | ||||||||||||||||||||||||
試料 |
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キーワード | tubulin auto-regulation mRNA decay translation SCAPER / RIBOSOME | ||||||||||||||||||||||||
機能・相同性 | 機能・相同性情報 ooplasm / odontoblast differentiation / positive regulation of mRNA catabolic process / sperm head / cytoskeleton-dependent intracellular transport / GTPase activating protein binding / regulation of G1 to G0 transition / exit from mitosis / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / regulation of translation involved in cellular response to UV ...ooplasm / odontoblast differentiation / positive regulation of mRNA catabolic process / sperm head / cytoskeleton-dependent intracellular transport / GTPase activating protein binding / regulation of G1 to G0 transition / exit from mitosis / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / regulation of translation involved in cellular response to UV / protein-DNA complex disassembly / natural killer cell mediated cytotoxicity / positive regulation of DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator / optic nerve development / antral ovarian follicle growth / intercellular bridge / retinal ganglion cell axon guidance / G1 to G0 transition / regulation of synapse organization / seminiferous tubule development / nuclear envelope lumen / MHC class I protein binding / positive regulation of signal transduction by p53 class mediator / microtubule-based process / ubiquitin ligase inhibitor activity / spindle assembly / protein-RNA complex assembly / cellular response to actinomycin D / negative regulation of ubiquitin-dependent protein catabolic process / rough endoplasmic reticulum / Loss of Nlp from mitotic centrosomes / Loss of proteins required for interphase microtubule organization from the centrosome / Recruitment of mitotic centrosome proteins and complexes / Recruitment of NuMA to mitotic centrosomes / maturation of LSU-rRNA / Anchoring of the basal body to the plasma membrane / DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest / cellular response to starvation / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / AURKA Activation by TPX2 / ribosomal large subunit biogenesis / positive regulation of translation / Regulation of TP53 Activity through Methylation / cellular response to gamma radiation / structural constituent of cytoskeleton / mitotic spindle / mRNA 5'-UTR binding / transcription coactivator binding / microtubule cytoskeleton organization / cytoplasmic ribonucleoprotein granule / rRNA processing / azurophil granule lumen / microtubule cytoskeleton / Regulation of PLK1 Activity at G2/M Transition / antimicrobial humoral immune response mediated by antimicrobial peptide / ribosome binding / regulation of translation / mitotic cell cycle / retina development in camera-type eye / 5S rRNA binding / large ribosomal subunit rRNA binding / ribosomal large subunit assembly / cell body / cytoplasmic vesicle / spermatogenesis / cytoplasmic translation / cytosolic large ribosomal subunit / defense response to Gram-negative bacterium / killing of cells of another organism / microtubule / Potential therapeutics for SARS / nucleic acid binding / tRNA binding / postsynaptic density / cytoskeleton / protein stabilization / rRNA binding / ribosome / structural constituent of ribosome / nuclear speck / mitochondrial matrix / ribonucleoprotein complex / translation / membrane raft / protein domain specific binding / cell division / DNA repair / GTPase activity / mRNA binding / DNA damage response / chromatin binding / ubiquitin protein ligase binding / positive regulation of cell population proliferation / Neutrophil degranulation / synapse / protein-containing complex binding / positive regulation of gene expression / GTP binding / nucleolus / structural molecule activity 類似検索 - 分子機能 | ||||||||||||||||||||||||
生物種 | Oryctolagus cuniculus (ウサギ) / Homo sapiens (ヒト) | ||||||||||||||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.8 Å | ||||||||||||||||||||||||
データ登録者 | Hopfler M / Absmeier E / Passmore LA / Hegde RS | ||||||||||||||||||||||||
資金援助 | 英国, European Union, ドイツ, スイス, 米国, 7件
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引用 | ジャーナル: Mol Cell / 年: 2023 タイトル: Mechanism of ribosome-associated mRNA degradation during tubulin autoregulation. 著者: Markus Höpfler / Eva Absmeier / Sew-Yeu Peak-Chew / Evangelia Vartholomaiou / Lori A Passmore / Ivana Gasic / Ramanujan S Hegde / 要旨: Microtubules play crucial roles in cellular architecture, intracellular transport, and mitosis. The availability of free tubulin subunits affects polymerization dynamics and microtubule function. ...Microtubules play crucial roles in cellular architecture, intracellular transport, and mitosis. The availability of free tubulin subunits affects polymerization dynamics and microtubule function. When cells sense excess free tubulin, they trigger degradation of the encoding mRNAs, which requires recognition of the nascent polypeptide by the tubulin-specific ribosome-binding factor TTC5. How TTC5 initiates the decay of tubulin mRNAs is unknown. Here, our biochemical and structural analysis reveals that TTC5 recruits the poorly studied protein SCAPER to the ribosome. SCAPER, in turn, engages the CCR4-NOT deadenylase complex through its CNOT11 subunit to trigger tubulin mRNA decay. SCAPER mutants that cause intellectual disability and retinitis pigmentosa in humans are impaired in CCR4-NOT recruitment, tubulin mRNA degradation, and microtubule-dependent chromosome segregation. Our findings demonstrate how recognition of a nascent polypeptide on the ribosome is physically linked to mRNA decay factors via a relay of protein-protein interactions, providing a paradigm for specificity in cytoplasmic gene regulation. | ||||||||||||||||||||||||
履歴 |
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-構造の表示
添付画像 |
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-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_16155.map.gz | 53.3 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-16155-v30.xml emd-16155.xml | 80.7 KB 80.7 KB | 表示 表示 | EMDBヘッダ |
FSC (解像度算出) | emd_16155_fsc.xml | 18.4 KB | 表示 | FSCデータファイル |
画像 | emd_16155.png | 197.8 KB | ||
マスクデータ | emd_16155_msk_1.map | 536.4 MB | マスクマップ | |
Filedesc metadata | emd-16155.cif.gz | 17 KB | ||
その他 | emd_16155_additional_1.map.gz emd_16155_additional_2.map.gz emd_16155_additional_3.map.gz emd_16155_half_map_1.map.gz emd_16155_half_map_2.map.gz | 432.9 MB 433.1 MB 432.6 MB 430 MB 429.9 MB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-16155 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-16155 | HTTPS FTP |
-検証レポート
文書・要旨 | emd_16155_validation.pdf.gz | 1 MB | 表示 | EMDB検証レポート |
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文書・詳細版 | emd_16155_full_validation.pdf.gz | 1 MB | 表示 | |
XML形式データ | emd_16155_validation.xml.gz | 26.4 KB | 表示 | |
CIF形式データ | emd_16155_validation.cif.gz | 35.2 KB | 表示 | |
アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-16155 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-16155 | HTTPS FTP |
-関連構造データ
関連構造データ | 8bpoMC M: このマップから作成された原子モデル C: 同じ文献を引用 (文献) |
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類似構造データ | 類似検索 - 機能・相同性F&H 検索 |
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_16155.map.gz / 形式: CCP4 / 大きさ: 536.4 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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注釈 | Sharpened map of the 60S subunit bound to SCAPER and TTC5 | ||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 0.829 Å | ||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-マスク #1
ファイル | emd_16155_msk_1.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-追加マップ: unsharpened map of the 80S ribosome after focussed...
ファイル | emd_16155_additional_1.map | ||||||||||||
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注釈 | unsharpened map of the 80S ribosome after focussed classification around the expansion segment | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-追加マップ: unsharpened map of the 80S ribosome after focussed...
ファイル | emd_16155_additional_2.map | ||||||||||||
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注釈 | unsharpened map of the 80S ribosome after focussed classification around the P-site tRNA | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-追加マップ: unsharpened map of the 80S ribosome after focussed...
ファイル | emd_16155_additional_3.map | ||||||||||||
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注釈 | unsharpened map of the 80S ribosome after focussed classification around SCAPER | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: Half-map 1 of the 60S subunit bound to SCAPER and TTC5
ファイル | emd_16155_half_map_1.map | ||||||||||||
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注釈 | Half-map 1 of the 60S subunit bound to SCAPER and TTC5 | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: Half-map 2 of the 60S subunit bound to SCAPER and TTC5
ファイル | emd_16155_half_map_2.map | ||||||||||||
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注釈 | Half-map 2 of the 60S subunit bound to SCAPER and TTC5 | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-試料の構成要素
+全体 : rabbit 80S ribosome translating beta-tubulin in complex with tetr...
+超分子 #1: rabbit 80S ribosome translating beta-tubulin in complex with tetr...
+分子 #1: 28S ribosomal RNA
+分子 #2: 5S ribosomal RNA
+分子 #3: 5.8S ribosomal RNA
+分子 #4: Nascent polypeptide-associated complex subunit alpha N-terminal region
+分子 #5: 60S ribosomal protein L8
+分子 #6: Ribosomal protein L3
+分子 #7: 60S ribosomal protein L4
+分子 #8: Ribosomal_L18_c domain-containing protein
+分子 #9: 60S ribosomal protein L6
+分子 #10: 60S ribosomal protein L7
+分子 #11: 60S ribosomal protein L7a
+分子 #12: 60S ribosomal protein L9
+分子 #13: Ribosomal protein L10
+分子 #14: 60S ribosomal protein L11
+分子 #15: 60S ribosomal protein L13
+分子 #16: 60S ribosomal protein L14
+分子 #17: Ribosomal protein L15
+分子 #18: 60S ribosomal protein L13a
+分子 #19: 60S ribosomal protein L17
+分子 #20: Ribosomal protein L18
+分子 #21: 60S ribosomal protein L19
+分子 #22: 60S ribosomal protein L18a
+分子 #23: 60S ribosomal protein L21
+分子 #24: 60S ribosomal protein L22
+分子 #25: 60S ribosomal protein L23
+分子 #26: Ribosomal protein L24
+分子 #27: Ribosomal_L23eN domain-containing protein
+分子 #28: Ribosomal protein L26
+分子 #29: 60S ribosomal protein L27
+分子 #30: 60S ribosomal protein L27a
+分子 #31: 60S ribosomal protein L29
+分子 #32: 60S ribosomal protein L30
+分子 #33: 60S ribosomal protein L31
+分子 #34: Ribosomal protein L32
+分子 #35: 60S ribosomal protein L35a
+分子 #36: 60S ribosomal protein L34
+分子 #37: 60S ribosomal protein L35
+分子 #38: 60S ribosomal protein L36
+分子 #39: Ribosomal protein L37
+分子 #40: 60S ribosomal protein L38
+分子 #41: 60S ribosomal protein L39-like
+分子 #42: Ubiquitin-60S ribosomal protein L40
+分子 #43: 60S ribosomal protein L41
+分子 #44: 60S ribosomal protein L36a-like
+分子 #45: 60S ribosomal protein L37a
+分子 #46: 60S ribosomal protein L28
+分子 #47: 60S acidic ribosomal protein P0
+分子 #48: 60S ribosomal protein L12
+分子 #49: S phase cyclin A-associated protein in the endoplasmic reticulum
+分子 #50: Tetratricopeptide repeat protein 5
+分子 #51: Tubulin beta chain
+分子 #52: MAGNESIUM ION
+分子 #53: ZINC ION
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7.4 |
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グリッド | モデル: UltrAuFoil R1.2/1.3 / 材質: GOLD / 支持フィルム - 材質: GRAPHENE OXIDE / 支持フィルム - トポロジー: CONTINUOUS / 前処理 - タイプ: GLOW DISCHARGE |
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 277 K / 装置: FEI VITROBOT MARK IV |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 撮影したグリッド数: 1 / 実像数: 20932 / 平均露光時間: 1.8 sec. / 平均電子線量: 44.7 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | C2レンズ絞り径: 70.0 µm / 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm / 最大 デフォーカス(公称値): 2.7 µm / 最小 デフォーカス(公称値): 1.2 µm / 倍率(公称値): 105000 |
試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER ホルダー冷却材: NITROGEN |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |