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- EMDB-16090: Structure of a yeast 80S ribosome-bound N-Acetyltransferase B complex -

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Basic information

Entry
Database: EMDB / ID: EMD-16090
TitleStructure of a yeast 80S ribosome-bound N-Acetyltransferase B complex
Map dataHomogenous Refinement of a class of 80S yeast ribosomes with two copies of the NatB complex. Half map.
Sample
  • Complex: Large ribosomal subunit of a yeast 80S ribosome with NatB-complex
    • Protein or peptide: x 43 types
    • RNA: x 3 types
  • Ligand: x 2 types
Function / homology
Function and homology information


N-terminal methionine Nalpha-acetyltransferase NatB / NatB complex / mitochondrion inheritance / N-terminal peptidyl-methionine acetylation / : / N-terminal protein amino acid acetylation / peptide alpha-N-acetyltransferase activity / pre-mRNA 5'-splice site binding / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / response to cycloheximide ...N-terminal methionine Nalpha-acetyltransferase NatB / NatB complex / mitochondrion inheritance / N-terminal peptidyl-methionine acetylation / : / N-terminal protein amino acid acetylation / peptide alpha-N-acetyltransferase activity / pre-mRNA 5'-splice site binding / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / response to cycloheximide / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Formation of a pool of free 40S subunits / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / negative regulation of mRNA splicing, via spliceosome / L13a-mediated translational silencing of Ceruloplasmin expression / preribosome, large subunit precursor / ribosomal large subunit export from nucleus / protein-RNA complex assembly / regulation of translational fidelity / translational termination / cytoskeleton organization / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / ribosomal large subunit biogenesis / regulation of actin cytoskeleton organization / macroautophagy / maintenance of translational fidelity / ribosomal large subunit assembly / modification-dependent protein catabolic process / rRNA processing / protein tag activity / ribosome biogenesis / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / rRNA binding / negative regulation of translation / ribosome / protein ubiquitination / structural constituent of ribosome / translation / response to antibiotic / mRNA binding / ubiquitin protein ligase binding / RNA binding / nucleus / metal ion binding / cytoplasm / cytosol
Similarity search - Function
N-acetyltransferase B complex, non-catalytic subunit / N-acetyltransferase B complex (NatB) non catalytic subunit / Acetyltransferase (GNAT) family / Ribosomal protein L41 / Ribosomal protein L41 / Gcn5-related N-acetyltransferase (GNAT) domain profile. / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. ...N-acetyltransferase B complex, non-catalytic subunit / N-acetyltransferase B complex (NatB) non catalytic subunit / Acetyltransferase (GNAT) family / Ribosomal protein L41 / Ribosomal protein L41 / Gcn5-related N-acetyltransferase (GNAT) domain profile. / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / GNAT domain / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L44e signature. / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L10e / Ribosomal protein L13e / Ribosomal protein L13e / Ribosomal protein L19, eukaryotic / 60S ribosomal protein L18a/ L20, eukaryotes / : / Ribosomal protein L24e, conserved site / Ribosomal protein L24e signature. / Ribosomal protein L44e / Ribosomal protein L19/L19e conserved site / Ribosomal protein L44 / Ribosomal protein L19e signature. / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L6e signature. / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal L40e family / Ribosomal protein L30e signature 1. / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / Ribosomal protein L30e signature 2. / Ribosomal protein L30e, conserved site / Ribosomal protein 60S L18 and 50S L18e / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal L27e protein family / Ribosomal protein L36e signature. / Ribosomal protein L39e, conserved site / Ribosomal protein L39e signature. / Ribosomal protein L34Ae / Ribosomal protein L34e / 60S ribosomal protein L19 / Ribosomal protein L35Ae, conserved site / Ribosomal protein L35Ae signature. / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / Ribosomal protein L30/YlxQ / 60S ribosomal protein L35 / Ribosomal Protein L6, KOW domain / Ribosomal protein L7A/L8 / Ribosomal protein L6e / Ribosomal protein L13, eukaryotic/archaeal / 60S ribosomal protein L6E / Ribosomal protein L18e / 60S ribosomal protein L4, C-terminal domain / 60S ribosomal protein L4 C-terminal domain / Ribosomal protein L37ae / Ribosomal protein L7, eukaryotic / Ribosomal protein L31e, conserved site / Ribosomal L37ae protein family / Ribosomal protein L31e signature. / Ribosomal_L19e / Ribosomal protein L30, N-terminal / Ribosomal L30 N-terminal domain / Ribosomal protein L19/L19e / Ribosomal protein L19/L19e, domain 1 / Ribosomal protein L19/L19e superfamily / Ribosomal protein L19e / Ribosomal protein L36e / Ribosomal protein L36e domain superfamily / Ribosomal protein L36e / Ribosomal protein L39e / Ribosomal protein L14e domain / Ribosomal protein L39e domain superfamily / Ribosomal L39 protein / Ribosomal protein L14 / Ribosomal protein L5 eukaryotic/L18 archaeal / Ribosomal large subunit proteins 60S L5, and 50S L18 / Ribosomal protein L35A / Ribosomal protein L35Ae
Similarity search - Domain/homology
Large ribosomal subunit protein uL15 / Large ribosomal subunit protein eL24A / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein uL30A / Large ribosomal subunit protein uL6A / Large ribosomal subunit protein eL6B / Large ribosomal subunit protein uL22A / Large ribosomal subunit protein uL24A / Large ribosomal subunit protein eL33A ...Large ribosomal subunit protein uL15 / Large ribosomal subunit protein eL24A / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein uL30A / Large ribosomal subunit protein uL6A / Large ribosomal subunit protein eL6B / Large ribosomal subunit protein uL22A / Large ribosomal subunit protein uL24A / Large ribosomal subunit protein eL33A / Large ribosomal subunit protein eL36A / Large ribosomal subunit protein eL29 / Large ribosomal subunit protein eL15A / Large ribosomal subunit protein eL22A / Large ribosomal subunit protein eL27A / Large ribosomal subunit protein eL31A / Ubiquitin-ribosomal protein eL40A fusion protein / Large ribosomal subunit protein eL20A / Large ribosomal subunit protein eL43A / Large ribosomal subunit protein eL42A / Large ribosomal subunit protein uL14A / Large ribosomal subunit protein uL2A / Large ribosomal subunit protein eL18A / Large ribosomal subunit protein eL19A / Large ribosomal subunit protein uL29A / Small ribosomal subunit protein eS32A / Large ribosomal subunit protein uL4A / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein eL8A / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL13A / Large ribosomal subunit protein eL14A / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein eL37A / Large ribosomal subunit protein eL38 / Large ribosomal subunit protein eL34A / Large ribosomal subunit protein eL21A / N-terminal acetyltransferase B complex catalytic subunit NAT3 / N-terminal acetyltransferase B complex subunit MDM20 / Large ribosomal subunit protein eL13A / Large ribosomal subunit protein uL5B
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (brewer's yeast) / baker's yeast (brewer's yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.8 Å
AuthorsKnorr AG / Mackens-Kiani T / Musial J / Berninghausen O / Becker T / Beatrix B / Beckmann R
Funding support Germany, 1 items
OrganizationGrant numberCountry
German Research Foundation (DFG) Germany
CitationJournal: PLoS Biol / Year: 2023
Title: The dynamic architecture of Map1- and NatB-ribosome complexes coordinates the sequential modifications of nascent polypeptide chains.
Authors: Alexandra G Knorr / Timur Mackens-Kiani / Joanna Musial / Otto Berninghausen / Thomas Becker / Birgitta Beatrix / Roland Beckmann /
Abstract: Cotranslational modification of the nascent polypeptide chain is one of the first events during the birth of a new protein. In eukaryotes, methionine aminopeptidases (MetAPs) cleave off the starter ...Cotranslational modification of the nascent polypeptide chain is one of the first events during the birth of a new protein. In eukaryotes, methionine aminopeptidases (MetAPs) cleave off the starter methionine, whereas N-acetyl-transferases (NATs) catalyze N-terminal acetylation. MetAPs and NATs compete with other cotranslationally acting chaperones, such as ribosome-associated complex (RAC), protein targeting and translocation factors (SRP and Sec61) for binding sites at the ribosomal tunnel exit. Yet, whereas well-resolved structures for ribosome-bound RAC, SRP and Sec61, are available, structural information on the mode of ribosome interaction of eukaryotic MetAPs or of the five cotranslationally active NATs is only available for NatA. Here, we present cryo-EM structures of yeast Map1 and NatB bound to ribosome-nascent chain complexes. Map1 is mainly associated with the dynamic rRNA expansion segment ES27a, thereby kept at an ideal position below the tunnel exit to act on the emerging substrate nascent chain. For NatB, we observe two copies of the NatB complex. NatB-1 binds directly below the tunnel exit, again involving ES27a, and NatB-2 is located below the second universal adapter site (eL31 and uL22). The binding mode of the two NatB complexes on the ribosome differs but overlaps with that of NatA and Map1, implying that NatB binds exclusively to the tunnel exit. We further observe that ES27a adopts distinct conformations when bound to NatA, NatB, or Map1, together suggesting a contribution to the coordination of a sequential activity of these factors on the emerging nascent chain at the ribosomal exit tunnel.
History
DepositionNov 5, 2022-
Header (metadata) releaseFeb 8, 2023-
Map releaseFeb 8, 2023-
UpdateMay 3, 2023-
Current statusMay 3, 2023Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_16090.png

Downloads & links

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Map

FileDownload / File: emd_16090.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationHomogenous Refinement of a class of 80S yeast ribosomes with two copies of the NatB complex. Half map.
Voxel sizeX=Y=Z: 1.045 Å
Density
Contour LevelBy AUTHOR: 0.2
Minimum - Maximum-0.4981414 - 1.3824084
Average (Standard dev.)0.011220821 (±0.0929733)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 417.99997 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: Homogenous Refinement of a class of 80S yeast...

Fileemd_16090_half_map_1.map
AnnotationHomogenous Refinement of a class of 80S yeast ribosomes with two copies of the NatB complex. Half map.
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Homogenous Refinement of a class of 80S yeast...

Fileemd_16090_half_map_2.map
AnnotationHomogenous Refinement of a class of 80S yeast ribosomes with two copies of the NatB complex. Half map.
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Large ribosomal subunit of a yeast 80S ribosome with NatB-complex

EntireName: Large ribosomal subunit of a yeast 80S ribosome with NatB-complex
Components
  • Complex: Large ribosomal subunit of a yeast 80S ribosome with NatB-complex
    • Protein or peptide: N-terminal acetyltransferase B complex catalytic subunit NAT3
    • Protein or peptide: N-terminal acetyltransferase B complex subunit MDM20
    • RNA: 5S rRNA
    • RNA: 5.8S rRNA
    • Protein or peptide: 60S ribosomal protein L2-A
    • Protein or peptide: 60S ribosomal protein L3
    • Protein or peptide: 60S ribosomal protein L4-A
    • Protein or peptide: 60S ribosomal protein L5
    • Protein or peptide: 60S ribosomal protein L6-B
    • Protein or peptide: 60S ribosomal protein L7-A
    • Protein or peptide: 60S ribosomal protein L8-A
    • Protein or peptide: 60S ribosomal protein L9-A
    • Protein or peptide: 60S ribosomal protein L10
    • Protein or peptide: 60S ribosomal protein L11-B
    • Protein or peptide: 60S ribosomal protein L13-A
    • Protein or peptide: 60S ribosomal protein L14-A
    • Protein or peptide: 60S ribosomal protein L15-A
    • Protein or peptide: 60S ribosomal protein L16-A
    • Protein or peptide: 60S ribosomal protein L17-A
    • Protein or peptide: 60S ribosomal protein L18-A
    • Protein or peptide: 60S ribosomal protein L19-A
    • Protein or peptide: 60S ribosomal protein L20-A
    • Protein or peptide: 60S ribosomal protein L21-A
    • Protein or peptide: 60S ribosomal protein L22-A
    • Protein or peptide: 60S ribosomal protein L23-A
    • Protein or peptide: 60S ribosomal protein L24-A
    • Protein or peptide: 60S ribosomal protein L25
    • Protein or peptide: 60S ribosomal protein L26-A
    • Protein or peptide: 60S ribosomal protein L27-A
    • Protein or peptide: 60S ribosomal protein L28
    • Protein or peptide: 60S ribosomal protein L29
    • Protein or peptide: 60S ribosomal protein L30
    • Protein or peptide: 60S ribosomal protein L31-A
    • Protein or peptide: 60S ribosomal protein L32
    • Protein or peptide: 60S ribosomal protein L33-A
    • Protein or peptide: 60S ribosomal protein L34-A
    • Protein or peptide: 60S ribosomal protein L35-A
    • Protein or peptide: 60S ribosomal protein L36-A
    • Protein or peptide: 60S ribosomal protein L37-A
    • Protein or peptide: 60S ribosomal protein L38
    • Protein or peptide: 60S ribosomal protein L39
    • Protein or peptide: 60S ribosomal protein L40-A
    • Protein or peptide: 60S ribosomal protein L41-A
    • Protein or peptide: 60S ribosomal protein L42-A
    • Protein or peptide: 60S ribosomal protein L43-A
    • RNA: 25S rRNA
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION

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Supramolecule #1: Large ribosomal subunit of a yeast 80S ribosome with NatB-complex

SupramoleculeName: Large ribosomal subunit of a yeast 80S ribosome with NatB-complex
type: complex / ID: 1 / Chimera: Yes / Parent: 0 / Macromolecule list: #1-#46
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)

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Macromolecule #1: N-terminal acetyltransferase B complex catalytic subunit NAT3

MacromoleculeName: N-terminal acetyltransferase B complex catalytic subunit NAT3
type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
EC number: N-terminal methionine Nalpha-acetyltransferase NatB
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 22.953254 KDa
SequenceString: MTTIQPFEPV DLFKTNNVNL DILTENFPLE FYFEYMIIWP DLFFKSSEMT VDPTFKHNIS GYMMAKTEGK TTEWHTHITA VTVAPRFRR ISLASKLCNT LETMTDVMPH EVNFIDLFVK CNNQLAIKLY EKLGYSVYRR VVGYYNSAED GYPDTLKKVD D NKDAFDMR ...String:
MTTIQPFEPV DLFKTNNVNL DILTENFPLE FYFEYMIIWP DLFFKSSEMT VDPTFKHNIS GYMMAKTEGK TTEWHTHITA VTVAPRFRR ISLASKLCNT LETMTDVMPH EVNFIDLFVK CNNQLAIKLY EKLGYSVYRR VVGYYNSAED GYPDTLKKVD D NKDAFDMR KAMARDRNRS VRPDGRSHKC YPHDVRF

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Macromolecule #2: N-terminal acetyltransferase B complex subunit MDM20

MacromoleculeName: N-terminal acetyltransferase B complex subunit MDM20 / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 92.930539 KDa
SequenceString: MSDKIQEEIL GLVSRSNFKQ CYAKLGQLQK QFPNALYFKI LETYVKFKQS PGKFDYNKLL EEPYGLKGTT ITGDTRSLEF LHNFFVELG KYDEALHVYE RGNFKFPSYE LSYHWFMKAL EDSNYNQMSK ASLQLAKYSD SGNLPKRAYY FWNAISILAV S RFQENTLS ...String:
MSDKIQEEIL GLVSRSNFKQ CYAKLGQLQK QFPNALYFKI LETYVKFKQS PGKFDYNKLL EEPYGLKGTT ITGDTRSLEF LHNFFVELG KYDEALHVYE RGNFKFPSYE LSYHWFMKAL EDSNYNQMSK ASLQLAKYSD SGNLPKRAYY FWNAISILAV S RFQENTLS DPKKILLSRL ARQSLLDLKP FQNVQEIIVY CLVLDELFPQ SREISEEIVA ITFANFDTSV NLYLKNFILK HT KLLNSPQ KLFEVCSKLI EKGLDDYELI TNLIDAAYKL SKSKDEVKQW IDENLGDSRN TRLARLKLDI MYTDSVSESS LSY YLSKYH NKPCCSIDLN HYSGHINIDM LKSIMSKYDP EDKDLIHHCN ILELGLIGSD SINNYNKFKG TLEKKSVTDY SSCS TFLLE IVKDKCKKTN PELKDVLLCI TILENYQAKD PHNFDTMCWL IVLYMYLGLV PDAYFHFINL KIKNVQTDSL DYMIF SRFS TLFPNKQSDF YSKTFHEHNN LYDTSLANLP RYIQVAFERN SYSKILGMLE MRDKLMKSYT RWTKTLENLQ FSRLCN DKR GHLLQKLHED WRSLEMTQSV SFSDNRDFSI LDENFAQFLN RGKILEYANL NEESIFLTLI RELIIEALPN GEKTEQI SA LLKKLPSINL EELLNNNLTE VESASFLIFF EIYENNGKNL HDLISRLMKV PINAKQNWMV SHTYLTKMAT LKTLDSLK R IKDKEIQKLI KNSLKELRSC CDDVFKGYSK ALVQAYEELK KDECGNLLKE LDVKAENVKN IKNSLLGIQK SVRNL

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Macromolecule #5: 60S ribosomal protein L2-A

MacromoleculeName: 60S ribosomal protein L2-A / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 27.089158 KDa
SequenceString: GRVIRNQRKG AGSIFTSHTR LRQGAAKLRT LDYAERHGYI RGIVKQIVHD SGRGAPLAKV VFRDPYKYRL REEIFIANEG VHTGQFIYA GKKASLNVGN VLPLGSVPEG TIVSNVEEKP GDRGALARAS GNYVIIIGHN PDENKTRVRL PSGAKKVISS D ARGVIGVI ...String:
GRVIRNQRKG AGSIFTSHTR LRQGAAKLRT LDYAERHGYI RGIVKQIVHD SGRGAPLAKV VFRDPYKYRL REEIFIANEG VHTGQFIYA GKKASLNVGN VLPLGSVPEG TIVSNVEEKP GDRGALARAS GNYVIIIGHN PDENKTRVRL PSGAKKVISS D ARGVIGVI AGGGRVDKPL LKAGRAFHKY RLKRNSWPKT RGVAMNPVDH PHGGGNHQHI GKASTISRGA VSGQKAGLIA AR RTGLLRG SQKT

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Macromolecule #6: 60S ribosomal protein L3

MacromoleculeName: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 43.719602 KDa
SequenceString: SHRKYEAPRH GHLGFLPRKR AASIRARVKA FPKDDRSKPV ALTSFLGYKA GMTTIVRDLD RPGSKFHKRE VVEAVTVVDT PPVVVVGVV GYVETPRGLR SLTTVWAEHL SDEVKRRFYK NWYKSKKKAF TKYSAKYAQD GAGIERELAR IKKYASVVRV L VHTQIRKT ...String:
SHRKYEAPRH GHLGFLPRKR AASIRARVKA FPKDDRSKPV ALTSFLGYKA GMTTIVRDLD RPGSKFHKRE VVEAVTVVDT PPVVVVGVV GYVETPRGLR SLTTVWAEHL SDEVKRRFYK NWYKSKKKAF TKYSAKYAQD GAGIERELAR IKKYASVVRV L VHTQIRKT PLAQKKAHLA EIQLNGGSIS EKVDWAREHF EKTVAVDSVF EQNEMIDAIA VTKGHGFEGV THRWGTKKLP RK THRGLRK VACIGAWHPA HVMWSVARAG QRGYHSRTSI NHKIYRVGKG DDEANGATSF DRTKKTITPM GGFVHYGEIK NDF IMVKGC IPGNRKRIVT LRKSLYTNTS RKALEEVSLK WIDTASKFGK GRFQTPAEKH AFMGTLKKDL

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Macromolecule #7: 60S ribosomal protein L4-A

MacromoleculeName: 60S ribosomal protein L4-A / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 39.027926 KDa
SequenceString: SRPQVTVHSL TGEATANALP LPAVFSAPIR PDIVHTVFTS VNKNKRQAYA VSEKAGHQTS AESWGTGRAV ARIPRVGGGG TGRSGQGAF GNMCRGGRMF APTKTWRKWN VKVNHNEKRY ATASAIAATA VASLVLARGH RVEKIPEIPL VVSTDLESIQ K TKEAVAAL ...String:
SRPQVTVHSL TGEATANALP LPAVFSAPIR PDIVHTVFTS VNKNKRQAYA VSEKAGHQTS AESWGTGRAV ARIPRVGGGG TGRSGQGAF GNMCRGGRMF APTKTWRKWN VKVNHNEKRY ATASAIAATA VASLVLARGH RVEKIPEIPL VVSTDLESIQ K TKEAVAAL KAVGAHSDLL KVLKSKKLRA GKGKYRNRRW TQRRGPLVVY AEDNGIVKAL RNVPGVETAN VASLNLLQLA PG AHLGRFV IWTEAAFTKL DQVWGSETVA SSKVGYTLPS HIISTSDVTR IINSSEIQSA IRPAGQATQK RTHVLKKNPL KNK QVLLRL NPYAKVFAAE KLGSKKAEKT GTKPAAVFTE TLKHD

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Macromolecule #8: 60S ribosomal protein L5

MacromoleculeName: 60S ribosomal protein L5 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 33.415387 KDa
SequenceString: QKDAKSSAYS SRFQTPFRRR REGKTDYYQR KRLVTQHKAK YNTPKYRLVV RFTNKDIICQ IISSTITGDV VLAAAYSHEL PRYGITHGL TNWAAAYATG LLIARRTLQK LGLDETYKGV EEVEGEYELT EAVEDGPRPF KVFLDIGLQR TTTGARVFGA L KGASDGGL ...String:
QKDAKSSAYS SRFQTPFRRR REGKTDYYQR KRLVTQHKAK YNTPKYRLVV RFTNKDIICQ IISSTITGDV VLAAAYSHEL PRYGITHGL TNWAAAYATG LLIARRTLQK LGLDETYKGV EEVEGEYELT EAVEDGPRPF KVFLDIGLQR TTTGARVFGA L KGASDGGL YVPHSENRFP GWDFETEEID PELLRSYIFG GHVSQYMEEL ADDDEERFSE LFKGYLADDI DADSLEDIYT SA HEAIRAD PAFKPTEKKF TKEQYAAESK KYRQTKLSKE ERAARVAAKI AALAGQQ

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Macromolecule #9: 60S ribosomal protein L6-B

MacromoleculeName: 60S ribosomal protein L6-B / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 19.881357 KDa
SequenceString:
TAQQAPKWYP SEDVAAPKKT RKAVRPQKLR ASLVPGTVLI LLAGRFRGKR VVYLKHLEDN TLLVTGPFKV NGVPLRRVNA RYVIATSTK VSVEGVNVEK FNVEYFAKEK LTKKEKKEAN LFPEQQTKEI KAERVEDQKV VDKALIAEIK KTPLLKQYLS A SFSLKNGD KPHMLKF

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Macromolecule #10: 60S ribosomal protein L7-A

MacromoleculeName: 60S ribosomal protein L7-A / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 25.240377 KDa
SequenceString: AEQVAAERAA RKAANKEKRA IILERNAAYQ KEYETAERNI IQAKRDAKAA GSYYVEAQHK LVFVVRIKGI NKIPPKPRKV LQLLRLTRI NSGTFVKVTK ATLELLKLIE PYVAYGYPSY STIRQLVYKR GFGKINKQRV PLSDNAIIEA NLGKYGILSI D DLIHEIIT ...String:
AEQVAAERAA RKAANKEKRA IILERNAAYQ KEYETAERNI IQAKRDAKAA GSYYVEAQHK LVFVVRIKGI NKIPPKPRKV LQLLRLTRI NSGTFVKVTK ATLELLKLIE PYVAYGYPSY STIRQLVYKR GFGKINKQRV PLSDNAIIEA NLGKYGILSI D DLIHEIIT VGPHFKQANN FLWPFKLSNP SGGWGVPRKF KHFIQGGSFG NREEFINKLV KSMN

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Macromolecule #11: 60S ribosomal protein L8-A

MacromoleculeName: 60S ribosomal protein L8-A / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 25.814998 KDa
SequenceString: NPLTHSTPKN FGIGQAVQPK RNLSRYVKWP EYVRVQRQKK ILSIRLKVPP TIAQFQYTLD RNTAAETFKL FNKYRPETAA EKKERLTKE AAAVAEGKSK QDASPKPYAV KYGLNHVVAL IENKKAKLVL IANDVDPIEL VVFLPALCKK MGVPYAIVKG K ARLGTLVN ...String:
NPLTHSTPKN FGIGQAVQPK RNLSRYVKWP EYVRVQRQKK ILSIRLKVPP TIAQFQYTLD RNTAAETFKL FNKYRPETAA EKKERLTKE AAAVAEGKSK QDASPKPYAV KYGLNHVVAL IENKKAKLVL IANDVDPIEL VVFLPALCKK MGVPYAIVKG K ARLGTLVN QKTSAVAALT EVRAEDEAAL AKLVSTIDAN FADKYDEVKK HWGGGILGNK AQAKMDKRAK NSDSA

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Macromolecule #12: 60S ribosomal protein L9-A

MacromoleculeName: 60S ribosomal protein L9-A / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 21.605061 KDa
SequenceString:
MKYIQTEQQI EVPEGVTVSI KSRIVKVVGP RGTLTKNLKH IDVTFTKVNN QLIKVAVHNG GRKHVAALRT VKSLVDNMIT GVTKGYKYK MRYVYAHFPI NVNIVEKDGA KFIEVRNFLG DKKIRNVPVR DGVTIEFSTN VKDEIVLSGN SVEDVSQNAA D LQQICRVR NKDIRKFLDG IYVSHKGFIT EDL

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Macromolecule #13: 60S ribosomal protein L10

MacromoleculeName: 60S ribosomal protein L10 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 25.080062 KDa
SequenceString: ARRPARCYRY QKNKPYPKSR YNRAVPDSKI RIYDLGKKKA TVDEFPLCVH LVSNELEQLS SEALEAARIC ANKYMTTVSG RDAFHLRVR VHPFHVLRIN KMLSCAGADR LQQGMRGAWG KPHGLAARVD IGQIIFSVRT KDSNKDVVVE GLRRARYKFP G QQKIILSK ...String:
ARRPARCYRY QKNKPYPKSR YNRAVPDSKI RIYDLGKKKA TVDEFPLCVH LVSNELEQLS SEALEAARIC ANKYMTTVSG RDAFHLRVR VHPFHVLRIN KMLSCAGADR LQQGMRGAWG KPHGLAARVD IGQIIFSVRT KDSNKDVVVE GLRRARYKFP G QQKIILSK KWGFTNLDRP EYLKKREAGE VKDDGAFVKF LSKKGSLENN IREFPEYFAA

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Macromolecule #14: 60S ribosomal protein L11-B

MacromoleculeName: 60S ribosomal protein L11-B / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 19.266082 KDa
SequenceString:
QNPMRDLKIE KLVLNISVGE SGDRLTRASK VLEQLSGQTP VQSKARYTVR TFGIRRNEKI AVHVTVRGPK AEEILERGLK VKEYQLRDR NFSATGNFGF GIDEHIDLGI KYDPSIGIFG MDFYVVMNRP GARVTRRKRC KGTVGNSHKT TKEDTVSWFK Q KYDADVLD K

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Macromolecule #15: 60S ribosomal protein L13-A

MacromoleculeName: 60S ribosomal protein L13-A / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 21.885234 KDa
SequenceString: AISKNLPILK NHFRKHWQER VKVHFDQAGK KVSRRNARAT RAAKIAPRPL DLLRPVVRAP TVKYNRKVRA GRGFTLAEVK AAGLTAAYA RTIGIAVDHR RQNRNQEIFD ANVQRLKEYQ SKIIVFPRNG KAPEAEQVLS AAATFPIAQP ATDVEARAVQ D NGESAFRT ...String:
AISKNLPILK NHFRKHWQER VKVHFDQAGK KVSRRNARAT RAAKIAPRPL DLLRPVVRAP TVKYNRKVRA GRGFTLAEVK AAGLTAAYA RTIGIAVDHR RQNRNQEIFD ANVQRLKEYQ SKIIVFPRNG KAPEAEQVLS AAATFPIAQP ATDVEARAVQ D NGESAFRT LRLARSEKKF RGIREKRARE KAEAE

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Macromolecule #16: 60S ribosomal protein L14-A

MacromoleculeName: 60S ribosomal protein L14-A / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 14.976794 KDa
SequenceString:
TDSIVKASNW RLVEVGRVVL IKKGQSAGKL AAIVEIIDQK KVLIDGPKAG VPRQAINLGQ VVLTPLTFAL PRGARTATVS KKWAAAAVC EKWAASSWAK KIAQRERRAA LTDFERFQVM VLRKQKRYTV KKALAKA

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Macromolecule #17: 60S ribosomal protein L15-A

MacromoleculeName: 60S ribosomal protein L15-A / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 24.351162 KDa
SequenceString: GAYKYLEELQ RKKQSDVLRF LQRVRVWEYR QKNVIHRAAR PTRPDKARRL GYKAKQGFVI YRVRVRRGNR KRPVPKGATY GKPTNQGVN ELKYQRSLRA TAEERVGRRA ANLRVLNSYW VNQDSTYKYF EVILVDPQHK AIRRDARYNW ICDPVHKHRE A RGLTATGK ...String:
GAYKYLEELQ RKKQSDVLRF LQRVRVWEYR QKNVIHRAAR PTRPDKARRL GYKAKQGFVI YRVRVRRGNR KRPVPKGATY GKPTNQGVN ELKYQRSLRA TAEERVGRRA ANLRVLNSYW VNQDSTYKYF EVILVDPQHK AIRRDARYNW ICDPVHKHRE A RGLTATGK KSRGINKGHK FNNTKAGRRK TWKRQNTLSL WRYRK

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Macromolecule #18: 60S ribosomal protein L16-A

MacromoleculeName: 60S ribosomal protein L16-A / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 22.028951 KDa
SequenceString: VEPVVVIDGK GHLVGRLASV VAKQLLNGQK IVVVRAEELN ISGEFFRNKL KYHDFLRKAT AFNKTRGPFH FRAPSRIFYK ALRGMVSHK TARGKAALER LKVFEGIPPP YDKKKRVVVP QALRVLRLKP GRKYTTLGKL STSVGWKYED VVAKLEAKRK V SSAEYYAK ...String:
VEPVVVIDGK GHLVGRLASV VAKQLLNGQK IVVVRAEELN ISGEFFRNKL KYHDFLRKAT AFNKTRGPFH FRAPSRIFYK ALRGMVSHK TARGKAALER LKVFEGIPPP YDKKKRVVVP QALRVLRLKP GRKYTTLGKL STSVGWKYED VVAKLEAKRK V SSAEYYAK KRAFTKKVAS ANATAAESDV AKQLAALGY

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Macromolecule #19: 60S ribosomal protein L17-A

MacromoleculeName: 60S ribosomal protein L17-A / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 20.458322 KDa
SequenceString:
ARYGATSTNP AKSASARGSY LRVSFKNTRE TAQAINGWEL TKAQKYLEQV LDHQRAIPFR RFNSSIGRTA QGKEFGVTKA RWPAKSVKF VQGLLQNAAA NAEAKGLDAT KLYVSHIQVN QAPKQRRRTY RAHGRINKYE SSPSHIELVV TEKEEAVAKA A EKKVVRLT SRQRGRIAAQ KRIAA

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Macromolecule #20: 60S ribosomal protein L18-A

MacromoleculeName: 60S ribosomal protein L18-A / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 20.478059 KDa
SequenceString:
GIDHTSKQHK RSGHRTAPKS DNVYLKLLVK LYTFLARRTD APFNKVVLKA LFLSKINRPP VSVSRIARAL KQEGAANKTV VVVGTVTDD ARIFEFPKTT VAALRFTAGA RAKIVKAGGE CITLDQLAVR APKGQNTLIL RGPRNSREAV RHFGMGPHKG K APRILSTG RKFERARGRR RSKGFKV

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Macromolecule #21: 60S ribosomal protein L19-A

MacromoleculeName: 60S ribosomal protein L19-A / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 21.631121 KDa
SequenceString:
ANLRTQKRLA ASVVGVGKRK VWLDPNETSE IAQANSRNAI RKLVKNGTIV KKAVTVHSKS RTRAHAQSKR EGRHSGYGKR KGTREARLP SQVVWIRRLR VLRRLLAKYR DAGKIDKHLY HVLYKESKGN AFKHKRALVE HIIQAKADAQ REKALNEEAE A RRLKNRAA RDRRAQRVAE KRDALLKEDA

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Macromolecule #22: 60S ribosomal protein L20-A

MacromoleculeName: 60S ribosomal protein L20-A / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 20.347654 KDa
SequenceString:
AHFKEYQVIG RRLPTESVPE PKLFRMRIFA SNEVIAKSRY WYFLQKLHKV KKASGEIVSI NQINEAHPTK VKNFGVWVRY DSRSGTHNM YKEIRDVSRV AAVETLYQDM AARHRARFRS IHILKVAEIE KTADVKRQYV KQFLTKDLKF PLPHRVQKST K TFSYKRPS TFY

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Macromolecule #23: 60S ribosomal protein L21-A

MacromoleculeName: 60S ribosomal protein L21-A / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 18.148066 KDa
SequenceString:
GKSHGYRSRT RYMFQRDFRK HGAVHLSTYL KVYKVGDIVD IKANGSIQKG MPHKFYQGKT GVVYNVTKSS VGVIINKMVG NRYLEKRLN LRVEHIKHSK CRQEFLERVK ANAAKRAEAK AQGVAVQLKR QPAQPRESRI VSTEGNVPQT LAPVPYETFI

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Macromolecule #24: 60S ribosomal protein L22-A

MacromoleculeName: 60S ribosomal protein L22-A / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 11.263863 KDa
SequenceString:
QKIAKTFTVD VSSPTENGVF DPASYAKYLI DHIKVEGAVG NLGNAVTVTE DGTVVTVVST AKFSGKYLKY LTKKYLKKNQ LRDWIRFVS TKTNEYRLAF Y

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Macromolecule #25: 60S ribosomal protein L23-A

MacromoleculeName: 60S ribosomal protein L23-A / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 14.362755 KDa
SequenceString:
SGNGAQGTKF RISLGLPVGA IMNCADNSGA RNLYIIAVKG SGSRLNRLPA ASLGDMVMAT VKKGKPELRK KVMPAIVVRQ AKSWRRRDG VFLYFEDNAG VIANPKGEMK GSAITGPVGK ECADLWPRVA SNSGVVV

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Macromolecule #26: 60S ribosomal protein L24-A

MacromoleculeName: 60S ribosomal protein L24-A / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 14.617215 KDa
SequenceString:
MKVEIDSFSG AKIYPGRGTL FVRGDSKIFR FQNSKSASLF KQRKNPRRIA WTVLFRKHHK KGITEEVAKK RSRKTVKAQR PITGASLDL IKERRSLKPE VRKAQREEKQ KADKEKKKAA KAARKAE

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Macromolecule #27: 60S ribosomal protein L25

MacromoleculeName: 60S ribosomal protein L25 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 13.771176 KDa
SequenceString:
KALKVRTSAT FRLPKTLKLA RAPKYASKAV PHYNRLDSYK VIEQPITSET AMKKVEDGNI LVFQVSMKAN KYQIKKAVKE LYEVDVLKV NTLVRPNGTK KAYVRLTADY DALDIANRIG YI

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Macromolecule #28: 60S ribosomal protein L26-A

MacromoleculeName: 60S ribosomal protein L26-A / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 14.005475 KDa
SequenceString:
AKQSLDVSSD RRKARKAYFT APSSQRRVLL SAPLSKELRA QYGIKALPIR RDDEVLVVRG SKKGQEGKIS SVYRLKFAVQ VDKVTKEKV NGASVPINLH PSKLVITKLH LDKDRKALIQ RKGGKL

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Macromolecule #29: 60S ribosomal protein L27-A

MacromoleculeName: 60S ribosomal protein L27-A / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 15.437166 KDa
SequenceString:
AKFLKAGKVA VVVRGRYAGK KVVIVKPHDE GSKSHPFGHA LVAGIERYPL KVTKKHGAKK VAKRTKIKPF IKVVNYNHLL PTRYTLDVE AFKSVVSTET FEQPSQREEA KKVVKKAFEE RHQAGKNQWF FSKLRF

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Macromolecule #30: 60S ribosomal protein L28

MacromoleculeName: 60S ribosomal protein L28 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 16.630471 KDa
SequenceString:
PSRFTKTRKH RGHVSAGKGR IGKHRKHPGG RGMAGGQHHH RINMDKYHPG YFGKVGMRYF HKQQAHFWKP VLNLDKLWTL IPEDKRDQY LKSASKETAP VIDTLAAGYG KILGKGRIPN VPVIVKARFV SKLAEEKIRA AGGVVELIA

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Macromolecule #31: 60S ribosomal protein L29

MacromoleculeName: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 6.560688 KDa
SequenceString:
AKSKNHTAHN QTRKAHRNGI KKPKTYKYPS LKGVDPKFRR NHKHALHGTA KALAAAKK

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Macromolecule #32: 60S ribosomal protein L30

MacromoleculeName: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 10.487266 KDa
SequenceString:
SINQKLALVI KSGKYTLGYK STVKSLRQGK SKLIIIAANT PVLRKSELEY YAMLSKTKVY YFQGGNNELG TAVGKLFRVG VVSILEAGD SDILTTL

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Macromolecule #33: 60S ribosomal protein L31-A

MacromoleculeName: 60S ribosomal protein L31-A / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 12.649754 KDa
SequenceString:
LKDVVTREYT INLHKRLHGV SFKKRAPRAV KEIKKFAKLH MGTDDVRLAP ELNQAIWKRG VKGVEYRLRL RISRKRNEEE DAKNPLFSY VEPVLVASAK GLQTVVVEED

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Macromolecule #34: 60S ribosomal protein L32

MacromoleculeName: 60S ribosomal protein L32 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 14.478054 KDa
SequenceString:
ASLPHPKIVK KHTKKFKRHH SDRYHRVAEN WRKQKGIDSV VRRRFRGNIS QPKIGYGSNK KTKFLSPSGH KTFLVANVKD LETLTMHTK TYAAEIAHNI SAKNRVVILA RAKALGIKVT NPKGRLAL

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Macromolecule #35: 60S ribosomal protein L33-A

MacromoleculeName: 60S ribosomal protein L33-A / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 12.045935 KDa
SequenceString:
AESHRLYVKG KHLSYQRSKR VNNPNVSLIK IEGVATPQDA QFYLGKRIAY VYRASKEVRG SKIRVMWGKV TRTHGNSGVV RATFRNNLP AKTFGASVRI FLYPSNI

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Macromolecule #36: 60S ribosomal protein L34-A

MacromoleculeName: 60S ribosomal protein L34-A / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 12.608831 KDa
SequenceString:
AQRVTFRRRN PYNTRSNKIK VVKTPGGILR AQHVKKLATR PKCGDCGSAL QGISTLRPRQ YATVSKTHKT VSRAYGGSRC ANCVKERII RAFLIEEQKI VKKVVKEQTE AAK

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Macromolecule #37: 60S ribosomal protein L35-A

MacromoleculeName: 60S ribosomal protein L35-A / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 13.811444 KDa
SequenceString:
AGVKAYELRT KSKEQLASQL VDLKKELAEL KVQKLSRPSL PKIKTVRKSI ACVLTVINEQ QREAVRQLYK GKKYQPKDLR AKKTRALRR ALTKFEASQV TEKQRKKQIA FPQRKYAIKA

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Macromolecule #38: 60S ribosomal protein L36-A

MacromoleculeName: 60S ribosomal protein L36-A / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 11.020063 KDa
SequenceString:
TVKTGIAIGL NKGKKVTSMT PAPKISYKKG AASNRTKFVR SLVREIAGLS PYERRLIDLI RNSGEKRARK VAKKRLGSFT RAKAKVEEM NNIIAASRRH

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Macromolecule #39: 60S ribosomal protein L37-A

MacromoleculeName: 60S ribosomal protein L37-A / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 9.588045 KDa
SequenceString:
GKGTPSFGKR HNKSHTLCNR CGRRSFHVQK KTCSSCGYPA AKTRSYNWGA KAKRRHTTGT GRMRYLKHVS RRFKNGFQTG SASKA

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Macromolecule #40: 60S ribosomal protein L38

MacromoleculeName: 60S ribosomal protein L38 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 8.714363 KDa
SequenceString:
AREITDIKQF LELTRRADVK TATVKINKKL NKAGKPFRQT KFKVRGSSSL YTLVINDAGK AKKLIQSLPP TLKVNRL

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Macromolecule #41: 60S ribosomal protein L39

MacromoleculeName: 60S ribosomal protein L39 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 6.227444 KDa
SequenceString:
AAQKSFRIKQ KMAKAKKQNR PLPQWIRLRT NNTIRYNAKR RNWRRTKMNI

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Macromolecule #42: 60S ribosomal protein L40-A

MacromoleculeName: 60S ribosomal protein L40-A / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 6.032321 KDa
SequenceString:
IIEPSLKALA SKYNCDKSVC RKCYARLPPR ATNCRKRKCG HTNQLRPKKK LK

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Macromolecule #43: 60S ribosomal protein L41-A

MacromoleculeName: 60S ribosomal protein L41-A / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 3.354243 KDa
SequenceString:
MRAKWRKKRT RRLKRKRRKV RARSK

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Macromolecule #44: 60S ribosomal protein L42-A

MacromoleculeName: 60S ribosomal protein L42-A / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 11.840159 KDa
SequenceString:
VNVPKTRKTY CKGKTCRKHT QHKVTQYKAG KASLFAQGKR RYDRKQSGFG GQTKPVFHKK AKTTKKVVLR LECVKCKTRA QLTLKRCKH FELGGEKKQK GQAL

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Macromolecule #45: 60S ribosomal protein L43-A

MacromoleculeName: 60S ribosomal protein L43-A / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 9.981756 KDa
SequenceString:
AKRTKKVGIT GKYGVRYGSS LRRQVKKLEI QQHARYDCSF CGKKTVKRGA AGIWTCSCCK KTVAGGAYTV STAAAATVRS TIRRLREMV EA

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Macromolecule #3: 5S rRNA

MacromoleculeName: 5S rRNA / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 38.951105 KDa
SequenceString:
GGUUGCGGCC AUAUCUACCA GAAAGCACCG UUUCCCGUCC GAUCAACUGU AGUUAAGCUG GUAAGAGCCU GACCGAGUAG UGUAGUGGG UGACCAUACG CGAAACUCAG GUGCUGCAAU CU

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Macromolecule #4: 5.8S rRNA

MacromoleculeName: 5.8S rRNA / type: rna / ID: 4 / Number of copies: 1
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 50.682922 KDa
SequenceString:
AAACUUUCAA CAACGGAUCU CUUGGUUCUC GCAUCGAUGA AGAACGCAGC GAAAUGCGAU ACGUAAUGUG AAUUGCAGAA UUCCGUGAA UCAUCGAAUC UUUGAACGCA CAUUGCGCCC CUUGGUAUUC CAGGGGGCAU GCCUGUUUGA GCGUCAUUU

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Macromolecule #46: 25S rRNA

MacromoleculeName: 25S rRNA / type: rna / ID: 46 / Number of copies: 1
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 1.097132625 MDa
SequenceString: GUUUGACCUC AAAUCAGGUA GGAGUACCCG CUGAACUUAA GCAUAUCAAU AAGCGGAGGA AAAGAAACCA ACCGGGAUUG CCUUAGUAA CGGCGAGUGA AGCGGCAAAA GCUCAAAUUU GAAAUCUGGU ACCUUCGGUG CCCGAGUUGU AAUUUGGAGA G GGCAACUU ...String:
GUUUGACCUC AAAUCAGGUA GGAGUACCCG CUGAACUUAA GCAUAUCAAU AAGCGGAGGA AAAGAAACCA ACCGGGAUUG CCUUAGUAA CGGCGAGUGA AGCGGCAAAA GCUCAAAUUU GAAAUCUGGU ACCUUCGGUG CCCGAGUUGU AAUUUGGAGA G GGCAACUU UGGGGCCGUU CCUUGUCUAU GUUCCUUGGA ACAGGACGUC AUAGAGGGUG AGAAUCCCGU GUGGCGAGGA GU GCGGUUC UUUGUAAAGU GCCUUCGAAG AGUCGAGUUG UUUGGGAAUG CAGCUCUAAG UGGGUGGUAA AUUCCAUCUA AAG CUAAAU AUUGGCGAGA GACCGAUAGC GAACAAGUAC AGUGAUGGAA AGAUGAAAAG AACUUUGAAA AGAGAGUGAA AAAG UACGU GAAAUUGUUG AAAGGGAAGG GCAUUUGAUC AGACAUGGUG UUUUGUGCCC UCUGCUCCUU GUGGGUAGGG GAAUC UCGC AUUUCACUGG CCAGCAUCAG UUUUGGUGGC AGGAUAAAUC CAUAGGAAUG UAGCUUGCCU CGGUAAGUAU UAUAGC CUG UGGGAAUACU GCCAGCUGGG ACUGAGGACU GCGACGUAAG UCAAGGAUGC UGGCAUAAUG GUUAUAUGCC GCCCGUC UU GAAACACGGA CCAAGGAGUC UAACGUCUAU GCGAGUGUUU GGGUGUAAAA CCCAUACGCG UAAUGAAAGU GAACGUAG G UUGGGGCCUC GCAAGAGGUG CACAAUCGAC CGAUCCUGAU GUCUUCGGAU GGAUUUGAGU AAGAGCAUAG CUGUUGGGA CCCGAAAGAU GGUGAACUAU GCCUGAAUAG GGUGAAGCCA GAGGAAACUC UGGUGGAGGC UCGUAGCGGU UCUGACGUGC AAAUCGAUC GUCGAAUUUG GGUAUAGGGG CGAAAGACUA AUCGAACCAU CUAGUAGCUG GUUCCUGCCG AAGUUUCCCU C AGGAUAGC AGAAGCUCGU AUCAGUUUUA UGAGGUAAAG CGAAUGAUUA GAGGUUCCGG GGUCGAAAUG ACCUUGACCU AU UCUCAAA CUUUAAAUAU GUAAGAAGUC CUUGUUACUU AAUUGAACGU GGACAUUUGA AUGAAGAGCU UUUAGUGGGC CAU UUUUGG UAAGCAGAAC UGGCGAUGCG GGAUGAACCG AACGUAGAGU UAAGGUGCCG GAAUACACGC UCAUCAGACA CCAC AAAAG GUGUUAGUUC AUCUAGACAG CCGGACGGUG GCCAUGGAAG UCGGAAUCCG CUAAGGAGUG UGUAACAACU CACCG GCCG AAUGAACUAG CCCUGAAAAU GGAUGGCGCU CAAGCGUGUU ACCUAUACUC UACCGUCAGG GUUGAUAUGA UGCCCU GAC GAGUAGGCAG GCGUGGAGGU CAGUGACGAA GCCUAGACCG UAAGGUCGGG UCGAACGGCC UCUAGUGCAG AUCUUGG UG GUAGUAGCAA AUAUUCAAAU GAGAACUUUG AAGACUGAAG UGGGGAAAGG UUCCACGUCA ACAGCAGUUG GACGUGGG U UAGUCGAUCC UAAGAGAUGG GGAAGCUCCG UUUCAAAGGC CUGAUUUUAU GCAGGCCACC AUCGAAAGGG AAUCCGGUU AAGAUUCCGG AACCUGGAUA UGGAUUCUUC ACGGUAACGU AACUGAAUGU GGAGACGUCG GCGCGAGCCC UGGGAGGAGU UAUCUUUUC UUCUUAACAG CUUAUCACCC CGGAAUUGGU UUAUCCGGAG AUGGGGUCUU AUGGCUGGAA GAGGCCAGCA C CUUUGCUG GCUCCGGUGC GCUUGUGACG GCCCGUGAAA AUCCACAGGA AGGAAUAGUU UUCAUGCCAG GUCGUACUGA UA ACCGCAG CAGGUCUCCA AGGUGAACAG CCUCUAGUUG AUAGAAUAAU GUAGAUAAGG GAAGUCGGCA AAAUAGAUCC GUA ACUUCG GGAUAAGGAU UGGCUCUAAG GGUCGGGUAG UGAGAGCCUU GGUCAGACGC AGCGGGCGUG CUUGUGGACU GCUU GGUGG GGCUUGCUCU GCUAGGCGGA CUACUUGCGU GCCUUGUUGU AGACGGCCUU GGUAGGUCUC UUGUAGACCG UCGCU UGCU ACAAUUAACG AUCAACUUAG AACUGGUACG GACAAGGGGA AUCUGACUGU CUAAUUAAAA CAUAGCAUUG CGAUGG UCA GAAAGUGAUG UUGACGCAAU GUGAUUUCUG CCCAGUGCUC UGAAUGUCAA AGUGAAGAAA UUCAACCAAG CGCGGGU AA ACGGCGGGAG UAACUAUGAC UCUCUUAAGG UAGCCAAAUG CCUCGUCAUC UAAUUAGUGA CGCGCAUGAA UGGAUUAA C GAGAUUCCCA CUGUCCCUAU CUACUAUCUA GCGAAACCAC AGCCAAGGGA ACGGGCUUGG CAGAAUCAGC GGGGAAAGA AGACCCUGUU GAGCUUGACU CUAGUUUGAC AUUGUGAAGA GACAUAGAGG GUGUAGAAUA AGUGGGAGCU UCGGCGCCAG UGAAAUACC ACUACCUUUA UAGUUUCUUU ACUUAUUCAA UGAAGCGGAG CUGGAAUUCA UUUUCCACGU UCUAGCAUUC A AGGUCCCA UUCGGGGCUG AUCCGGGUUG AAGACAUUGU CAGGUGGGGA GUUUGGCUGG GGCGGCACAU CUGUUAAACG AU AACGCAG AUGUCCUAAG GGGGGCUCAU GGAGAACAGA AAUCUCCAGU AGAACAAAAG GGUAAAAGCC CCCUUGAUUU UGA UUUUCA GUGUGAAUAC AAACCAUGAA AGUGUGGCCU AUCGAUCCUU UAGUCCCUCG GAAUUUGAGG CUAGAGGUGC CAGA AAAGU UACCACAGGG AUAACUGGCU UGUGGCAGUC AAGCGUUCAU AGCGACAUUG CUUUUUGAUU CUUCGAUGUC GGCUC UUCC UAUCAUACCG AAGCAGAAUU CGGUAAGCGU UGGAUUGUUC ACCCACUAAU AGGGAACGUG AGCUGGGUUU AGACCG UCG UGAGACAGGU UAGUUUUACC CUACUGAUGA AUGUUACCGC AAUAGUAAUU GAACUUAGUA CGAGAGGAAC AGUUCAU UC GGAUAAUUGG UUUUUGCGGC UGUCUGAUCA GGCAUUGCCG CGAAGCUACC AUCCGCUGGA UUAUGGCUGA ACGCCUCU A AGUCAGAAUC CAUGCUAGAA CGCGGUGAUU UCUUUGCUCC ACACAAUAUA GAUGGAUACG AAUAAGGCGU CCUUGUGGC GUCGCUGAAC CAUAGCAGGC UAGCAACGGU GCACUUGGCG GAAAGGCCUU GGGUGCUUGC UGGCGAAUUG CAAUGUCAUU UUGCGUGGG GAUAAAUCAU UUGUAUACGA CUUAGAUGUA CAACGGGGUA UUGUAAGCAG UAGAGUAGCC UUGUUGUUAC G AUCUGCUG AGAUUAAGCC UUUGUUGUCU GAUUUGU

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Macromolecule #47: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 47 / Number of copies: 206 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #48: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 48 / Number of copies: 5 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 45.2 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.5 µm / Nominal defocus min: 0.5 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 9645
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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