[English] 日本語
Yorodumi- EMDB-16064: Cryo-electron tomogram of an extracellular vesicle containing tau... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-16064 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Cryo-electron tomogram of an extracellular vesicle containing tau filaments isolated from Alzheimer's Disease patient brain | |||||||||
Map data | ||||||||||
Sample |
| |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | electron tomography / cryo EM | |||||||||
Authors | Behr TS / Ryskeldi-Falcon B | |||||||||
Funding support | United Kingdom, 1 items
| |||||||||
Citation | Journal: bioRxiv / Year: 2023 Title: Tau filaments are tethered within brain extracellular vesicles in Alzheimer's disease. Authors: S L Fowler / T S Behr / E Turkes / P Maglio Cauhy / M S Foiani / A Schaler / G Crowley / S Bez / E Ficulle / E Tsefou / D P O'Brien / R Fischer / B Geary / P Gaur / C Miller / P D'Acunzo / E ...Authors: S L Fowler / T S Behr / E Turkes / P Maglio Cauhy / M S Foiani / A Schaler / G Crowley / S Bez / E Ficulle / E Tsefou / D P O'Brien / R Fischer / B Geary / P Gaur / C Miller / P D'Acunzo / E Levy / K E Duff / B Ryskeldi-Falcon Abstract: The abnormal assembly of tau protein in neurons is the pathological hallmark of multiple neurodegenerative diseases, including Alzheimer's disease (AD). In addition, assembled tau associates with ...The abnormal assembly of tau protein in neurons is the pathological hallmark of multiple neurodegenerative diseases, including Alzheimer's disease (AD). In addition, assembled tau associates with extracellular vesicles (EVs) in the central nervous system of patients with AD, which is linked to its clearance and prion-like propagation between neurons. However, the identities of the assembled tau species and the EVs, as well as how they associate, are not known. Here, we combined quantitative mass spectrometry, cryo-electron tomography and single-particle cryo-electron microscopy to study brain EVs from AD patients. We found filaments of truncated tau enclosed within EVs enriched in endo-lysosomal proteins. We observed multiple filament interactions, including with molecules that tethered filaments to the EV limiting membrane, suggesting selective packaging. Our findings will guide studies into the molecular mechanisms of EV-mediated secretion of assembled tau and inform the targeting of EV-associated tau as potential therapeutic and biomarker strategies for AD. | |||||||||
History |
|
-Structure visualization
Supplemental images |
---|
-Downloads & links
-EMDB archive
Map data | emd_16064.map.gz | 9.9 MB | EMDB map data format | |
---|---|---|---|---|
Header (meta data) | emd-16064-v30.xml emd-16064.xml | 8.6 KB 8.6 KB | Display Display | EMDB header |
Images | emd_16064.png | 130.7 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-16064 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-16064 | HTTPS FTP |
-Validation report
Summary document | emd_16064_validation.pdf.gz | 460.5 KB | Display | EMDB validaton report |
---|---|---|---|---|
Full document | emd_16064_full_validation.pdf.gz | 460.1 KB | Display | |
Data in XML | emd_16064_validation.xml.gz | 4 KB | Display | |
Data in CIF | emd_16064_validation.cif.gz | 4.4 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-16064 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-16064 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
---|
-Map
File | Download / File: emd_16064.map.gz / Format: CCP4 / Size: 10.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Voxel size | X=Y=Z: 16 Å | ||||||||||||||||||||
Density |
| ||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
|
-Supplemental data
-Sample components
-Entire : Extracellular vesicle
Entire | Name: Extracellular vesicle |
---|---|
Components |
|
-Supramolecule #1: Extracellular vesicle
Supramolecule | Name: Extracellular vesicle / type: organelle_or_cellular_component / ID: 1 / Parent: 0 |
---|---|
Source (natural) | Organism: Homo sapiens (human) |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
Processing | electron tomography |
Aggregation state | tissue |
-Sample preparation
Buffer | pH: 7.4 |
---|---|
Vitrification | Cryogen name: ETHANE |
Sectioning | Other: NO SECTIONING |
Fiducial marker | Manufacturer: BBI solutions / Diameter: 10 nm |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
---|---|
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 3.17 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 6.0 µm / Nominal defocus min: 3.0 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Final reconstruction | Software - Name: Warp (ver. 1.0.9) Details: Tomograms were reconstructed and filtered using Wiener-like deconvolution in WARP. Number images used: 34 |
---|