+
Open data
-
Basic information
Entry | ![]() | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Title | Structure of the IFT-A complex; IFT-A1 module | |||||||||||||||
![]() | IFT-A1 module; unsharpened map | |||||||||||||||
![]() |
| |||||||||||||||
![]() | cilia / intraflagellar transport / membrane protein import / complex / transport protein | |||||||||||||||
Function / homology | ![]() smoothened signaling pathway involved in dorsal/ventral neural tube patterning / myotome development / ear morphogenesis / intraciliary anterograde transport / cone photoreceptor outer segment / digestive system development / intraciliary transport particle A / embryonic heart tube left/right pattern formation / embryonic body morphogenesis / neural tube patterning ...smoothened signaling pathway involved in dorsal/ventral neural tube patterning / myotome development / ear morphogenesis / intraciliary anterograde transport / cone photoreceptor outer segment / digestive system development / intraciliary transport particle A / embryonic heart tube left/right pattern formation / embryonic body morphogenesis / neural tube patterning / photoreceptor cell outer segment organization / protein localization to ciliary membrane / intraciliary retrograde transport / establishment of protein localization to organelle / embryonic camera-type eye development / intraciliary transport / gonad development / spinal cord dorsal/ventral patterning / regulation of cilium assembly / photoreceptor connecting cilium / ciliary tip / Intraflagellar transport / camera-type eye morphogenesis / embryonic brain development / non-motile cilium assembly / protein localization to cilium / regulation of smoothened signaling pathway / embryonic heart tube development / embryonic cranial skeleton morphogenesis / non-motile cilium / embryonic forelimb morphogenesis / motile cilium / nervous system process / determination of left/right symmetry / embryonic limb morphogenesis / limb development / embryonic digit morphogenesis / receptor clustering / axoneme / photoreceptor outer segment / cilium assembly / Hedgehog 'off' state / negative regulation of smoothened signaling pathway / centriole / ciliary basal body / neural tube closure / cell morphogenesis / cilium / heart development / protein-containing complex assembly / in utero embryonic development / cytoskeleton / intracellular signal transduction / centrosome / membrane / plasma membrane / cytoplasm Similarity search - Function | |||||||||||||||
Biological species | ![]() | |||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 8.0 Å | |||||||||||||||
![]() | Hesketh SJ / Mukhopadhyay AG / Nakamura D / Toropova K / Roberts AJ | |||||||||||||||
Funding support | ![]()
| |||||||||||||||
![]() | ![]() Title: IFT-A structure reveals carriages for membrane protein transport into cilia. Authors: Sophie J Hesketh / Aakash G Mukhopadhyay / Dai Nakamura / Katerina Toropova / Anthony J Roberts / ![]() Abstract: Intraflagellar transport (IFT) trains are massive molecular machines that traffic proteins between cilia and the cell body. Each IFT train is a dynamic polymer of two large complexes (IFT-A and -B) ...Intraflagellar transport (IFT) trains are massive molecular machines that traffic proteins between cilia and the cell body. Each IFT train is a dynamic polymer of two large complexes (IFT-A and -B) and motor proteins, posing a formidable challenge to mechanistic understanding. Here, we reconstituted the complete human IFT-A complex and obtained its structure using cryo-EM. Combined with AlphaFold prediction and genome-editing studies, our results illuminate how IFT-A polymerizes, interacts with IFT-B, and uses an array of β-propeller and TPR domains to create "carriages" of the IFT train that engage TULP adaptor proteins. We show that IFT-A⋅TULP carriages are essential for cilia localization of diverse membrane proteins, as well as ICK-the key kinase regulating IFT train turnaround. These data establish a structural link between IFT-A's distinct functions, provide a blueprint for IFT-A in the train, and shed light on how IFT evolved from a proto-coatomer ancestor. | |||||||||||||||
History |
|
-
Structure visualization
Supplemental images |
---|
-
Downloads & links
-EMDB archive
Map data | ![]() | 252.6 MB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 20.4 KB 20.4 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 14.8 KB | Display | ![]() |
Images | ![]() | 75.4 KB | ||
Masks | ![]() | 512 MB | ![]() | |
Filedesc metadata | ![]() | 8.3 KB | ||
Others | ![]() ![]() | 475.8 MB 475.8 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 698.8 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 698.4 KB | Display | |
Data in XML | ![]() | 23.9 KB | Display | |
Data in CIF | ![]() | 30.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8bbfMC ![]() 8bbeC ![]() 8bbgC C: citing same article ( M: atomic model generated by this map |
---|---|
Similar structure data | Similarity search - Function & homology ![]() |
-
Links
EMDB pages | ![]() ![]() |
---|---|
Related items in Molecule of the Month |
-
Map
File | ![]() | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | IFT-A1 module; unsharpened map | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.067 Å | ||||||||||||||||||||
Density |
| ||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
|
-Supplemental data
-Mask #1
File | ![]() | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Half map: IFT-A1 module, half map A
File | emd_15955_half_map_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | IFT-A1 module, half map A | ||||||||||||
Projections & Slices |
| ||||||||||||
Density Histograms |
-Half map: IFT-A1 module, half map B
File | emd_15955_half_map_2.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | IFT-A1 module, half map B | ||||||||||||
Projections & Slices |
| ||||||||||||
Density Histograms |
-
Sample components
-Entire : IFT-A complex; IFT-A1 module
Entire | Name: IFT-A complex; IFT-A1 module |
---|---|
Components |
|
-Supramolecule #1: IFT-A complex; IFT-A1 module
Supramolecule | Name: IFT-A complex; IFT-A1 module / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
---|---|
Source (natural) | Organism: ![]() |
-Macromolecule #1: WD repeat-containing protein 19
Macromolecule | Name: WD repeat-containing protein 19 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO |
---|---|
Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 153.639297 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MKRIFSLLEK TWLGAPIQFA WQKTSGNYLA VTGADYIVKI FDRHGQKRSE INLPGNCVAM DWDKDGDVLA VIAEKSSCIY LWDANTNKT SQLDNGMRDQ MSFLLWSKVG SFLAVGTVKG NLLIYNHQTS RKIPVLGKHT KRITCGCWNA ENLLALGGED K MITVSNQE ...String: MKRIFSLLEK TWLGAPIQFA WQKTSGNYLA VTGADYIVKI FDRHGQKRSE INLPGNCVAM DWDKDGDVLA VIAEKSSCIY LWDANTNKT SQLDNGMRDQ MSFLLWSKVG SFLAVGTVKG NLLIYNHQTS RKIPVLGKHT KRITCGCWNA ENLLALGGED K MITVSNQE GDTIRQTQVR SEPSNMQFFL MKMDDRTSAA ESMISVVLGK KTLFFLNLNE PDNPADLEFQ QDFGNIVCYN WY GDGRIMI GFSCGHFVVI STHTGELGQE IFQARNHKDN LTSIAVSQTL NKVATCGDNC IKIQDLVDLK DMYVILNLDE ENK GLGTLS WTDDGQLLAL STQRGSLHVF LTKLPILGDA CSTRIAYLTS LLEVTVANPV EGELPITVSV DVEPNFVAVG LYHL AVGMN NRAWFYVLGE NAVKKLKDME YLGTVASICL HSDYAAALFE GKVQLHLIES EILDAQEERE TRLFPAVDDK CRILC HALT SDFLIYGTDT GVVQYFYIED WQFVNDYRHP VSVKKIFPDP NGTRLVFIDE KSDGFVYCPV NDATYEIPDF SPTIKG VLW ENWPMDKGVF IAYDDDKVYT YVFHKDTIQG AKVILAGSTK VPFAHKPLLL YNGELTCQTQ SGKVNNIYLS THGFLSN LK DTGPDELRPM LAQNLMLKRF SDAWEMCRIL NDEAAWNELA RACLHHMEVE FAIRVYRRIG NVGIVMSLEQ IKGIEDYN L LAGHLAMFTN DYNLAQDLYL ASSCPIAALE MRRDLQHWDS ALQLAKHLAP DQIPFISKEY AIQLEFAGDY VNALAHYEK GITGDNKEHD EACLAGVAQM SIRMGDIRRG VNQALKHPSR VLKRDCGAIL ENMKQFSEAA QLYEKGLYYD KAASVYIRSK NWAKVGDLL PHVSSPKIHL QYAKAKEADG RYKEAVVAYE NAKQWQSVIR IYLDHLNNPE KAVNIVRETQ SLDGAKMVAR F FLQLGDYG SAIQFLVMSK CNNEAFTLAQ QHNKMEIYAD IIGSEDTTNE DYQSIALYFE GEKRYLQAGK FFLLCGQYSR AL KHFLKCP SSEDNVAIEM AIETVGQAKD ELLTNQLIDH LLGENDGMPK DAKYLFRLYM ALKQYREAAQ TAIIIAREEQ SAG NYRNAH DVLFSMYAEL KSQKIKIPSE MATNLMILHS YILVKIHVKN GDHMKGARML IRVANNISKF PSHIVPILTS TVIE CHRAG LKNSAFSFAA MLMRPEYRSK IDAKYKKKIE GMVRRPDISE IEEATTPCPF CKFLLPECEL LCPGCKNSIP YCIAT GRHM LKDDWTVCPH CDFPALYSEL KIMLNTESTC PMCSERLNAA QLKKISDCTQ YLRTEEELEN LYFQSDYKDD DDK UniProtKB: WD repeat-containing protein 19 |
-Macromolecule #2: Intraflagellar transport protein 140 homolog
Macromolecule | Name: Intraflagellar transport protein 140 homolog / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO |
---|---|
Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 167.328312 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MALYYDHQIE APDAAGSPSF ISWHPVHPFL AVAYISTTST GSVDIYLEQG ECVPDTHVER PFRVASLCWH PTRLVLAVGW ETGEVTVFN KQDKEQHTMP LTHTADITVL RWSPSGNCLL SGDRLGVLLL WRLDQRGRVQ GTPLLKHEYG KHLTHCIFRL P PPGEDLVQ ...String: MALYYDHQIE APDAAGSPSF ISWHPVHPFL AVAYISTTST GSVDIYLEQG ECVPDTHVER PFRVASLCWH PTRLVLAVGW ETGEVTVFN KQDKEQHTMP LTHTADITVL RWSPSGNCLL SGDRLGVLLL WRLDQRGRVQ GTPLLKHEYG KHLTHCIFRL P PPGEDLVQ LAKAAVSGDE KALDMFNWKK SSSGSLLKMG SHEGLLFFVS LMDGTVHYVD EKGKTTQVVS ADSTIQMLFY ME KREALVV VTENLRLSLY TVPPEGKAEE VMKVKLSGKT GRRADIALIE GSLLVMAVGE AALRFWDIER GENYILSPDE KFG FEKGEN MNCVCYCKVK GLLAAGTDRG RVAMWRKVPD FLGSPGAEGK DRWALQTPTE LQGNITQIQW GSRKNLLAVN SVIS VAILS ERAMSSHFHQ QVAAMQVSPS LLNVCFLSTG VAHSLRTDMH ISGVFATKDA VAVWNGRQVA IFELSGAAIR SAGTF LCET PVLAMHEENV YTVESNRVQV RTWQGTVKQL LLFSETEGNP CFLDICGNFL VVGTDLAHFK SFDLSRREAK AHCSCR SLA ELVPGVGGIA SLRCSSSGST ISILPSKADN SPDSKICFYD VEMDTVTVFD FKTGQIDRRE TLSFNEQETN KSHLFVD EG LKNYVPVNHF WDQSEPRLFV CEAVQETPRS QPQSANGQPQ DGRAGPAADV LILSFFISEE HGFLLHESFP RPATSHSL L GMEVPYYYFT RKPEEADRED EVEPGCHHIP QMVSRRPLRD FVGLEDCDKA TRDAMLHFSF FVTIGDMDEA FKSIKLIKS EAVWENMARM CVKTQRLDVA KVCLGNMGHA RGARALREAE QEPELEARVA VLATQLGMLE DAEQLYRKCK RHDLLNKFYQ AAGRWQEAL QVAEHHDRVH LRSTYHRYAG HLEASADCSR ALSYYEKSDT HRFEVPRMLS EDLPSLELYV NKMKDKTLWR W WAQYLESQ GEMDAALHYY ELARDHFSLV RIHCFQGNVQ KAAQIANETG NLAASYHLAR QYESQEEVGQ AVHFYTRAQA FK NAIRLCK ENGLDDQLMN LALLSSPEDM IEAARYYEEK GVQMDRAVML YHKAGHFSKA LELAFATQQF VALQLIAEDL DET SDPALL ARCSDFFIEH SQYERAVELL LAARKYQEAL QLCLGQNMSI TEEMAEKMTV AKDSSDLPEE SRRELLEQIA DCCM RQGSY HLATKKYTQA GNKLKAMRAL LKSGDTEKIT FFASVSRQKE IYIMAANYLQ SLDWRKEPEI MKNIIGFYTK GRALD LLAG FYDACAQVEI DEYQNYDKAH GALTEAYKCL AKAKAKSPLD QETRLAQLQS RMALVKRFIQ ARRTYTEDPK ESIKQC ELL LEEPDLDSTI RIGDVYGFLV EHYVRKEEYQ TAYRFLEEMR RRLPLANMSY YVSPQAVDAV HRGLGLPLPR TVPEQVR HN SMEDARELDE EVVEEADDDP ENLYFQSWSH PQFEK UniProtKB: Intraflagellar transport protein 140 homolog |
-Macromolecule #3: Intraflagellar transport protein 122 homolog
Macromolecule | Name: Intraflagellar transport protein 122 homolog / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO |
---|---|
Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 142.007734 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MRAVLTWRDK AEHCINDIAF KPDGTQLILA AGSRLLVYDT SDGTLLQPLK GHKDTVYCVA YAKDGKRFAS GSADKSVIIW TSKLEGILK YTHNDAIQCV SYNPITHQLA SCSSSDFGLW SPEQKSVSKH KSSSKIICCS WTNDGQYLAL GMFNGIISIR N KNGEEKVK ...String: MRAVLTWRDK AEHCINDIAF KPDGTQLILA AGSRLLVYDT SDGTLLQPLK GHKDTVYCVA YAKDGKRFAS GSADKSVIIW TSKLEGILK YTHNDAIQCV SYNPITHQLA SCSSSDFGLW SPEQKSVSKH KSSSKIICCS WTNDGQYLAL GMFNGIISIR N KNGEEKVK IERPGGSLSP IWSICWNPSS RWESFWMNRE NEDAEDVIVN RYIQEIPSTL KSAVYSSQGS EAEEEEPEEE DD SPRDDNL EERNDILAVA DWGQKVSFYQ LSGKQIGKDR ALNFDPCCIS YFTKGEYILL GGSDKQVSLF TKDGVRLGTV GEQ NSWVWT CQAKPDSNYV VVGCQDGTIS FYQLIFSTVH GLYKDRYAYR DSMTDVIVQH LITEQKVRIK CKELVKKIAI YRNR LAIQL PEKILIYELY SEDLSDMHYR VKEKIIKKFE CNLLVVCANH IILCQEKRLQ CLSFSGVKER EWQMESLIRY IKVIG GPPG REGLLVGLKN GQILKIFVDN LFAIVLLKQA TAVRCLDMSA SRKKLAVVDE NDTCLVYDID TKELLFQEPN ANSVAW NTQ CEDMLCFSGG GYLNIKASTF PVHRQKLQGF VVGYNGSKIF CLHVFSISAV EVPQSAPMYQ YLDRKLFKEA YQIACLG VT DTDWRELAME ALEGLDFETA KKAFIRVQDL RYLELISSIE ERKKRGETNN DLFLADVFSY QGKFHEAAKL YKRSGHEN L ALEMYTDLCM FEYAKDFLGS GDPKETKMLI TKQADWARNI KEPKAAVEMY ISAGEHVKAI EICGDHGWVD MLIDIARKL DKAEREPLLL CATYLKKLDS PGYAAETYLK MGDLKSLVQL HVETQRWDEA FALGEKHPEF KDDIYMPYAQ WLAENDRFEE AQKAFHKAG RQREAVQVLE QLTNNAVAES RFNDAAYYYW MLSMQCLDIA QDPAQKDTML GKFYHFQRLA ELYHGYHAIH R HTEDPFSV HRPETLFNIS RFLLHSLPKD TPSGISKVKI LFTLAKQSKA LGAYRLARHA YDKLRGLYIP ARFQKSIELG TL TIRAKPF HDSEELVPLC YRCSTNNPLL NNLGNVCINC RQPFIFSASS YDVLHLVEFY LEEGITDEEA ISLIDLEVLR PKR DDRQLE IANNSSQILR LVETKDSIGD EDPFTAKLSF EQGGSEFVPV VVSRLVLRSM SRRDVLIKRW PPPLRWQYFR SLLP DASIT MCPSCFQMFH SEDYELLVLQ HGCCPYCRRC KDDPGP UniProtKB: Intraflagellar transport protein 122 homolog |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
![]() | single particle reconstruction |
Aggregation state | particle |
-
Sample preparation
Buffer | pH: 7.5 |
---|---|
Vitrification | Cryogen name: ETHANE / Instrument: FEI VITROBOT MARK IV |
-
Electron microscopy
Microscope | FEI TITAN KRIOS |
---|---|
Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 48.0 e/Å2 Details: Average electron dose for additional dataset was 49.5 (e-/A2) |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.5 µm / Nominal defocus min: 1.5 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |