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- EMDB-15426: Yeast RQC complex in state D -

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Basic information

Entry
Database: EMDB / ID: EMD-15426
TitleYeast RQC complex in state D
Map dataYeast RQC complex in state D, half map B
Sample
  • Complex: Yeast RQC complex in state D
    • Protein or peptide: x 48 types
    • RNA: x 4 types
  • Ligand: x 3 types
Function / homology
Function and homology information


RQC complex / Antigen processing: Ubiquitination & Proteasome degradation / ribosome-associated ubiquitin-dependent protein catabolic process / maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / pre-mRNA 5'-splice site binding / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / response to cycloheximide / maturation of 5.8S rRNA / ribosomal subunit export from nucleus / SRP-dependent cotranslational protein targeting to membrane ...RQC complex / Antigen processing: Ubiquitination & Proteasome degradation / ribosome-associated ubiquitin-dependent protein catabolic process / maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / pre-mRNA 5'-splice site binding / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / response to cycloheximide / maturation of 5.8S rRNA / ribosomal subunit export from nucleus / SRP-dependent cotranslational protein targeting to membrane / 90S preribosome / GTP hydrolysis and joining of the 60S ribosomal subunit / Formation of a pool of free 40S subunits / ribosomal large subunit binding / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / negative regulation of mRNA splicing, via spliceosome / protein-RNA complex assembly / subtelomeric heterochromatin formation / preribosome, large subunit precursor / L13a-mediated translational silencing of Ceruloplasmin expression / ribosomal large subunit export from nucleus / regulation of translational fidelity / rescue of stalled ribosome / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / ribosomal large subunit biogenesis / translation initiation factor activity / assembly of large subunit precursor of preribosome / cytosolic ribosome / cytosolic ribosome assembly / proteasomal protein catabolic process / macroautophagy / protein catabolic process / modification-dependent protein catabolic process / RING-type E3 ubiquitin transferase / ribosomal large subunit assembly / protein tag activity / rRNA processing / ubiquitin-protein transferase activity / ubiquitin protein ligase activity / large ribosomal subunit rRNA binding / ribosome biogenesis / cytoplasmic translation / chromatin organization / 5S rRNA binding / cytosolic large ribosomal subunit / ubiquitin-dependent protein catabolic process / negative regulation of translation / chromosome, telomeric region / rRNA binding / protein ubiquitination / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / response to antibiotic / mRNA binding / ubiquitin protein ligase binding / nucleolus / RNA binding / zinc ion binding / metal ion binding / nucleus / cytosol / cytoplasm
Similarity search - Function
NFACT protein, C-terminal / NFACT protein C-terminal domain / E3 ubiquitin-protein ligase listerin / Listerin, zinc finger, RING-type / FANCL C-terminal domain / Zinc finger, RING-CH-type / The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity. / NFACT, RNA-binding domain / NFACT protein RNA binding domain / NFACT N-terminal and middle domains ...NFACT protein, C-terminal / NFACT protein C-terminal domain / E3 ubiquitin-protein ligase listerin / Listerin, zinc finger, RING-type / FANCL C-terminal domain / Zinc finger, RING-CH-type / The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity. / NFACT, RNA-binding domain / NFACT protein RNA binding domain / NFACT N-terminal and middle domains / Ring finger domain / 60s Acidic ribosomal protein / 60S acidic ribosomal protein P0 / Translation initiation factor IF6 / eIF-6 family / translation initiation factor 6 / Ribosomal protein L41 / Ribosomal protein L41 / 50S ribosomal protein L10, insertion domain superfamily / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L10e, conserved site / Ribosomal protein L10e / Ribosomal protein L27e, conserved site / : / Ribosomal protein L24e, conserved site / Ribosomal protein L34e, conserved site / Ribosomal protein L1, conserved site / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein L44e / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L38e protein family / Ribosomal L22e protein family / Ribosomal protein L23/L25, N-terminal / 60S ribosomal protein L35 / Ribosomal protein L1 / Ribosomal protein L35Ae, conserved site / Ribosomal protein L30e, conserved site / Ribosomal protein L44 / Ribosomal protein L34Ae / Ribosomal protein L23, N-terminal domain / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal L27e protein family / Ribosomal Protein L6, KOW domain / Ribosomal protein L30/YlxQ / Ribosomal protein L13e / Ribosomal protein L13e / Ribosomal protein L34e / Ribosomal protein L31e, conserved site / Ribosomal protein L1 signature. / Ribosomal protein L37ae / Ribosomal protein L14e domain / 60S ribosomal protein L6E / Ribosomal protein L19, eukaryotic / Ribosomal protein L35A / Ribosomal protein L36e / Ribosomal protein L36e domain superfamily / Ribosomal L40e family / Ribosomal protein L36e / Ribosomal protein L35A superfamily / Ribosomal protein L44e signature. / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Ribosomal protein L7A/L8 / Ribosomal protein L27e signature. / Ribosomal protein L10e signature. / Ribosomal protein L32e, conserved site / 60S ribosomal protein L4, C-terminal domain / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein L7, eukaryotic / Ribosomal protein L6e / Ribosomal protein L14 / Ribosomal protein L30, N-terminal / Ribosomal protein L6, conserved site-2 / Ribosomal protein L14, KOW motif / Ribosomal L30 N-terminal domain / Ribosomal protein L19/L19e conserved site / Ribosomal protein L14 / Ribosomal L37ae protein family / Ribosomal protein L1, 3-layer alpha/beta-sandwich / Ribosomal protein L35Ae / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein L39e, conserved site / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / 60S ribosomal protein L4 C-terminal domain / Ribosomal protein L19e signature. / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein L24e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L34e signature.
Similarity search - Domain/homology
RPL10 isoform 1 / 60S ribosomal protein L29 / RPL5 isoform 1 / Large ribosomal subunit protein uL3 / RQC2 isoform 1 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein eL24A / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein uL10 ...RPL10 isoform 1 / 60S ribosomal protein L29 / RPL5 isoform 1 / Large ribosomal subunit protein uL3 / RQC2 isoform 1 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein eL24A / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein uL10 / Large ribosomal subunit protein uL30A / Large ribosomal subunit protein uL6A / Large ribosomal subunit protein eL6B / Large ribosomal subunit protein uL22A / Large ribosomal subunit protein uL24A / Large ribosomal subunit protein eL33A / Large ribosomal subunit protein eL36A / Large ribosomal subunit protein eL15A / Large ribosomal subunit protein eL22A / Large ribosomal subunit protein uL5A / Large ribosomal subunit protein eL27A / Large ribosomal subunit protein eL31A / Ubiquitin-ribosomal protein eL40A fusion protein / Large ribosomal subunit protein eL20A / Large ribosomal subunit protein eL43A / Large ribosomal subunit protein eL42A / Large ribosomal subunit protein uL14A / Large ribosomal subunit protein uL1A / Large ribosomal subunit protein uL2A / Large ribosomal subunit protein eL18A / Large ribosomal subunit protein uL11A / Large ribosomal subunit protein eL19A / Large ribosomal subunit protein uL29A / Small ribosomal subunit protein eS32A / Large ribosomal subunit protein uL4A / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein eL8A / Large ribosomal subunit protein uL13A / Large ribosomal subunit protein eL14A / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein eL37A / Large ribosomal subunit protein eL38 / Large ribosomal subunit protein eL34A / Large ribosomal subunit protein eL21A / E3 ubiquitin-protein ligase listerin / Eukaryotic translation initiation factor 6 / Large ribosomal subunit protein eL13A
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (brewer's yeast) / baker's yeast (brewer's yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.6 Å
AuthorsTesina P / Buschauer R / Beckmann R
Funding support Germany, 1 items
OrganizationGrant numberCountry
German Research Foundation (DFG) Germany
CitationJournal: Mol Cell / Year: 2023
Title: Molecular basis of eIF5A-dependent CAT tailing in eukaryotic ribosome-associated quality control.
Authors: Petr Tesina / Shuhei Ebine / Robert Buschauer / Matthias Thoms / Yoshitaka Matsuo / Toshifumi Inada / Roland Beckmann /
Abstract: Ribosome-associated quality control (RQC) is a conserved process degrading potentially toxic truncated nascent peptides whose malfunction underlies neurodegeneration and proteostasis decline in aging. ...Ribosome-associated quality control (RQC) is a conserved process degrading potentially toxic truncated nascent peptides whose malfunction underlies neurodegeneration and proteostasis decline in aging. During RQC, dissociation of stalled ribosomes is followed by elongation of the nascent peptide with alanine and threonine residues, driven by Rqc2 independently of mRNA, the small ribosomal subunit and guanosine triphosphate (GTP)-hydrolyzing factors. The resulting CAT tails (carboxy-terminal tails) and ubiquitination by Ltn1 mark nascent peptides for proteasomal degradation. Here we present ten cryogenic electron microscopy (cryo-EM) structures, revealing the mechanistic basis of individual steps of the CAT tailing cycle covering initiation, decoding, peptidyl transfer, and tRNA translocation. We discovered eIF5A as a crucial eukaryotic RQC factor enabling peptidyl transfer. Moreover, we observed dynamic behavior of RQC factors and tRNAs allowing for processivity of the CAT tailing cycle without additional energy input. Together, these results elucidate key differences as well as common principles between CAT tailing and canonical translation.
History
DepositionJul 20, 2022-
Header (metadata) releaseMar 8, 2023-
Map releaseMar 8, 2023-
UpdateMar 8, 2023-
Current statusMar 8, 2023Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_15426.png

Downloads & links

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Map

FileDownload / File: emd_15426.map.gz / Format: CCP4 / Size: 347.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationYeast RQC complex in state D, half map B
Projections & slices

Image control

Size
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AxesZ (Sec.)Y (Row.)X (Col.)
1.06 Å/pix.
x 450 pix.
= 476.55 Å		1.06 Å/pix.
x 450 pix.
= 476.55 Å		1.06 Å/pix.
x 450 pix.
= 476.55 Å

Surface

Projections

Slices (1/3)

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Images are generated by Spider.

Voxel sizeX=Y=Z: 1.059 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.55
Minimum - Maximum-0.6556004 - 3.7928455
Average (Standard dev.)0.020836378 (±0.1312815)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions450450450
Spacing450450450
CellA=B=C: 476.55002 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: Yeast RQC complex in state D, half map A

Fileemd_15426_half_map_1.map
AnnotationYeast RQC complex in state D, half map A
Projections & Slices
AxesZYX

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Half map: Yeast RQC complex in state D

Fileemd_15426_half_map_2.map
AnnotationYeast RQC complex in state D
Projections & Slices
AxesZYX

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Sample components

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Entire : Yeast RQC complex in state D

EntireName: Yeast RQC complex in state D
Components
  • Complex: Yeast RQC complex in state D
    • Protein or peptide: 60S ribosomal protein L15-ARibosome
    • Protein or peptide: 60S ribosomal protein L16-ARibosome
    • Protein or peptide: 60S ribosomal protein L17-ARibosome
    • Protein or peptide: 60S ribosomal protein L18-ARibosome
    • Protein or peptide: 60S ribosomal protein L19-ARibosome
    • Protein or peptide: 60S ribosomal protein L20-ARibosome
    • Protein or peptide: 60S ribosomal protein L21-ARibosome
    • Protein or peptide: 60S ribosomal protein L22-ARibosome
    • Protein or peptide: 60S ribosomal protein L23-ARibosome
    • Protein or peptide: 60S ribosomal protein L24-ARibosome
    • Protein or peptide: 60S ribosomal protein L25
    • Protein or peptide: 60S ribosomal protein L26-ARibosome
    • Protein or peptide: 60S ribosomal protein L27-ARibosome
    • Protein or peptide: 60S ribosomal protein L28
    • Protein or peptide: 60S ribosomal protein L29
    • Protein or peptide: 60S ribosomal protein L30
    • Protein or peptide: 60S ribosomal protein L31-ARibosome
    • Protein or peptide: 60S ribosomal protein L32
    • Protein or peptide: 60S ribosomal protein L33-ARibosome
    • Protein or peptide: 60S ribosomal protein L34-ARibosome
    • Protein or peptide: 60S ribosomal protein L35-ARibosome
    • Protein or peptide: 60S ribosomal protein L36-ARibosome
    • Protein or peptide: 60S ribosomal protein L37-ARibosome
    • Protein or peptide: 60S ribosomal protein L38
    • Protein or peptide: 60S ribosomal protein L39
    • Protein or peptide: Ubiquitin-60S ribosomal protein L40
    • Protein or peptide: 60S ribosomal protein L42-ARibosome
    • Protein or peptide: 60S ribosomal protein L43-ARibosome
    • Protein or peptide: 60S ribosomal protein L41-ARibosome
    • RNA: 25S rRNA
    • RNA: 5S rRNA5S ribosomal RNA
    • RNA: 5.8S rRNA5.8S ribosomal RNA
    • Protein or peptide: 60S ribosomal protein L2-ARibosome
    • Protein or peptide: 60S ribosomal protein L3
    • Protein or peptide: 60S ribosomal protein L4-ARibosome
    • Protein or peptide: 60S ribosomal protein L5
    • Protein or peptide: 60S ribosomal protein L6-BRibosome
    • Protein or peptide: 60S ribosomal protein L7-ARibosome
    • Protein or peptide: 60S ribosomal protein L8-ARibosome
    • Protein or peptide: 60S ribosomal protein L9-ARibosome
    • Protein or peptide: 60S ribosomal protein L10
    • Protein or peptide: 60S ribosomal protein L11-ARibosome
    • Protein or peptide: 60S ribosomal protein L13-ARibosome
    • Protein or peptide: 60S ribosomal protein L14-ARibosome
    • Protein or peptide: RQC2 isoform 1
    • Protein or peptide: E3 ubiquitin-protein ligase listerin
    • Protein or peptide: Eukaryotic translation initiation factor 6
    • RNA: Ala tRNA
    • Protein or peptide: 60S ribosomal protein L12-ARibosome
    • Protein or peptide: 60S acidic ribosomal protein P0
    • Protein or peptide: CAT-tailed nascent peptide
    • Protein or peptide: 60S ribosomal protein L1-ARibosome
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION
  • Ligand: SPERMIDINE

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Supramolecule #1: Yeast RQC complex in state D

SupramoleculeName: Yeast RQC complex in state D / type: complex / ID: 1 / Chimera: Yes / Parent: 0 / Macromolecule list: #1-#52
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)

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Macromolecule #1: 60S ribosomal protein L15-A

MacromoleculeName: 60S ribosomal protein L15-A / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 24.482357 KDa
SequenceString: MGAYKYLEEL QRKKQSDVLR FLQRVRVWEY RQKNVIHRAA RPTRPDKARR LGYKAKQGFV IYRVRVRRGN RKRPVPKGAT YGKPTNQGV NELKYQRSLR ATAEERVGRR AANLRVLNSY WVNQDSTYKY FEVILVDPQH KAIRRDARYN WICDPVHKHR E ARGLTATG ...String:
MGAYKYLEEL QRKKQSDVLR FLQRVRVWEY RQKNVIHRAA RPTRPDKARR LGYKAKQGFV IYRVRVRRGN RKRPVPKGAT YGKPTNQGV NELKYQRSLR ATAEERVGRR AANLRVLNSY WVNQDSTYKY FEVILVDPQH KAIRRDARYN WICDPVHKHR E ARGLTATG KKSRGINKGH KFNNTKAGRR KTWKRQNTLS LWRYRK

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Macromolecule #2: 60S ribosomal protein L16-A

MacromoleculeName: 60S ribosomal protein L16-A / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 22.247227 KDa
SequenceString: MSVEPVVVID GKGHLVGRLA SVVAKQLLNG QKIVVVRAEE LNISGEFFRN KLKYHDFLRK ATAFNKTRGP FHFRAPSRIF YKALRGMVS HKTARGKAAL ERLKVFEGIP PPYDKKKRVV VPQALRVLRL KPGRKYTTLG KLSTSVGWKY EDVVAKLEAK R KVSSAEYY ...String:
MSVEPVVVID GKGHLVGRLA SVVAKQLLNG QKIVVVRAEE LNISGEFFRN KLKYHDFLRK ATAFNKTRGP FHFRAPSRIF YKALRGMVS HKTARGKAAL ERLKVFEGIP PPYDKKKRVV VPQALRVLRL KPGRKYTTLG KLSTSVGWKY EDVVAKLEAK R KVSSAEYY AKKRAFTKKV ASANATAAES DVAKQLAALG Y

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Macromolecule #3: 60S ribosomal protein L17-A

MacromoleculeName: 60S ribosomal protein L17-A / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 20.589518 KDa
SequenceString:
MARYGATSTN PAKSASARGS YLRVSFKNTR ETAQAINGWE LTKAQKYLEQ VLDHQRAIPF RRFNSSIGRT AQGKEFGVTK ARWPAKSVK FVQGLLQNAA ANAEAKGLDA TKLYVSHIQV NQAPKQRRRT YRAHGRINKY ESSPSHIELV VTEKEEAVAK A AEKKVVRL TSRQRGRIAA QKRIAA

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Macromolecule #4: 60S ribosomal protein L18-A

MacromoleculeName: 60S ribosomal protein L18-A / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 20.609252 KDa
SequenceString:
MGIDHTSKQH KRSGHRTAPK SDNVYLKLLV KLYTFLARRT DAPFNKVVLK ALFLSKINRP PVSVSRIARA LKQEGAANKT VVVVGTVTD DARIFEFPKT TVAALRFTAG ARAKIVKAGG ECITLDQLAV RAPKGQNTLI LRGPRNSREA VRHFGMGPHK G KAPRILST GRKFERARGR RRSKGFKV

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Macromolecule #5: 60S ribosomal protein L19-A

MacromoleculeName: 60S ribosomal protein L19-A / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 21.762316 KDa
SequenceString:
MANLRTQKRL AASVVGVGKR KVWLDPNETS EIAQANSRNA IRKLVKNGTI VKKAVTVHSK SRTRAHAQSK REGRHSGYGK RKGTREARL PSQVVWIRRL RVLRRLLAKY RDAGKIDKHL YHVLYKESKG NAFKHKRALV EHIIQAKADA QREKALNEEA E ARRLKNRA ARDRRAQRVA EKRDALLKED A

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Macromolecule #6: 60S ribosomal protein L20-A

MacromoleculeName: 60S ribosomal protein L20-A / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 20.478852 KDa
SequenceString:
MAHFKEYQVI GRRLPTESVP EPKLFRMRIF ASNEVIAKSR YWYFLQKLHK VKKASGEIVS INQINEAHPT KVKNFGVWVR YDSRSGTHN MYKEIRDVSR VAAVETLYQD MAARHRARFR SIHILKVAEI EKTADVKRQY VKQFLTKDLK FPLPHRVQKS T KTFSYKRP STFY

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Macromolecule #7: 60S ribosomal protein L21-A

MacromoleculeName: 60S ribosomal protein L21-A / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 18.279266 KDa
SequenceString:
MGKSHGYRSR TRYMFQRDFR KHGAVHLSTY LKVYKVGDIV DIKANGSIQK GMPHKFYQGK TGVVYNVTKS SVGVIINKMV GNRYLEKRL NLRVEHIKHS KCRQEFLERV KANAAKRAEA KAQGVAVQLK RQPAQPRESR IVSTEGNVPQ TLAPVPYETF I

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Macromolecule #8: 60S ribosomal protein L22-A

MacromoleculeName: 60S ribosomal protein L22-A / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 13.711359 KDa
SequenceString:
MAPNTSRKQK IAKTFTVDVS SPTENGVFDP ASYAKYLIDH IKVEGAVGNL GNAVTVTEDG TVVTVVSTAK FSGKYLKYLT KKYLKKNQL RDWIRFVSTK TNEYRLAFYQ VTPEEDEEED EE

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Macromolecule #9: 60S ribosomal protein L23-A

MacromoleculeName: 60S ribosomal protein L23-A / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 14.49395 KDa
SequenceString:
MSGNGAQGTK FRISLGLPVG AIMNCADNSG ARNLYIIAVK GSGSRLNRLP AASLGDMVMA TVKKGKPELR KKVMPAIVVR QAKSWRRRD GVFLYFEDNA GVIANPKGEM KGSAITGPVG KECADLWPRV ASNSGVVV

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Macromolecule #10: 60S ribosomal protein L24-A

MacromoleculeName: 60S ribosomal protein L24-A / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 17.661717 KDa
SequenceString:
MKVEIDSFSG AKIYPGRGTL FVRGDSKIFR FQNSKSASLF KQRKNPRRIA WTVLFRKHHK KGITEEVAKK RSRKTVKAQR PITGASLDL IKERRSLKPE VRKANREEKL KANKEKKKAE KAARKAEKAK SAGTQSSKFS KQQAKGAFQK VAATSR

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Macromolecule #11: 60S ribosomal protein L25

MacromoleculeName: 60S ribosomal protein L25 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 15.787612 KDa
SequenceString:
MAPSAKATAA KKAVVKGTNG KKALKVRTSA TFRLPKTLKL ARAPKYASKA VPHYNRLDSY KVIEQPITSE TAMKKVEDGN ILVFQVSMK ANKYQIKKAV KELYEVDVLK VNTLVRPNGT KKAYVRLTAD YDALDIANRI GYI

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Macromolecule #12: 60S ribosomal protein L26-A

MacromoleculeName: 60S ribosomal protein L26-A / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 14.265784 KDa
SequenceString:
MAKQSLDVSS DRRKARKAYF TAPSSQRRVL LSAPLSKELR AQYGIKALPI RRDDEVLVVR GSKKGQEGKI SSVYRLKFAV QVDKVTKEK VNGASVPINL HPSKLVITKL HLDKDRKALI QRKGGKLE

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Macromolecule #13: 60S ribosomal protein L27-A

MacromoleculeName: 60S ribosomal protein L27-A / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 15.56836 KDa
SequenceString:
MAKFLKAGKV AVVVRGRYAG KKVVIVKPHD EGSKSHPFGH ALVAGIERYP LKVTKKHGAK KVAKRTKIKP FIKVVNYNHL LPTRYTLDV EAFKSVVSTE TFEQPSQREE AKKVVKKAFE ERHQAGKNQW FFSKLRF

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Macromolecule #14: 60S ribosomal protein L28

MacromoleculeName: 60S ribosomal protein L28 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 16.761666 KDa
SequenceString:
MPSRFTKTRK HRGHVSAGKG RIGKHRKHPG GRGMAGGQHH HRINMDKYHP GYFGKVGMRY FHKQQAHFWK PVLNLDKLWT LIPEDKRDQ YLKSASKETA PVIDTLAAGY GKILGKGRIP NVPVIVKARF VSKLAEEKIR AAGGVVELIA

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Macromolecule #15: 60S ribosomal protein L29

MacromoleculeName: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 6.691884 KDa
SequenceString:
MAKSKNHTAH NQTRKAHRNG IKKPKTYKYP SLKGVDPKFR RNHKHALHGT AKALAAAKK

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Macromolecule #16: 60S ribosomal protein L30

MacromoleculeName: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 11.430364 KDa
SequenceString:
MAPVKSQESI NQKLALVIKS GKYTLGYKST VKSLRQGKSK LIIIAANTPV LRKSELEYYA MLSKTKVYYF QGGNNELGTA VGKLFRVGV VSILEAGDSD ILTTLA

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Macromolecule #17: 60S ribosomal protein L31-A

MacromoleculeName: 60S ribosomal protein L31-A / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 12.980158 KDa
SequenceString:
MAGLKDVVTR EYTINLHKRL HGVSFKKRAP RAVKEIKKFA KLHMGTDDVR LAPELNQAIW KRGVKGVEYR LRLRISRKRN EEEDAKNPL FSYVEPVLVA SAKGLQTVVV EEDA

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Macromolecule #18: 60S ribosomal protein L32

MacromoleculeName: 60S ribosomal protein L32 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 14.809441 KDa
SequenceString:
MASLPHPKIV KKHTKKFKRH HSDRYHRVAE NWRKQKGIDS VVRRRFRGNI SQPKIGYGSN KKTKFLSPSG HKTFLVANVK DLETLTMHT KTYAAEIAHN ISAKNRVVIL ARAKALGIKV TNPKGRLALE A

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Macromolecule #19: 60S ribosomal protein L33-A

MacromoleculeName: 60S ribosomal protein L33-A / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 12.17713 KDa
SequenceString:
MAESHRLYVK GKHLSYQRSK RVNNPNVSLI KIEGVATPQD AQFYLGKRIA YVYRASKEVR GSKIRVMWGK VTRTHGNSGV VRATFRNNL PAKTFGASVR IFLYPSNI

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Macromolecule #20: 60S ribosomal protein L34-A

MacromoleculeName: 60S ribosomal protein L34-A / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 13.673196 KDa
SequenceString:
MAQRVTFRRR NPYNTRSNKI KVVKTPGGIL RAQHVKKLAT RPKCGDCGSA LQGISTLRPR QYATVSKTHK TVSRAYGGSR CANCVKERI IRAFLIEEQK IVKKVVKEQT EAAKKSEKKA KK

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Macromolecule #21: 60S ribosomal protein L35-A

MacromoleculeName: 60S ribosomal protein L35-A / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 13.94264 KDa
SequenceString:
MAGVKAYELR TKSKEQLASQ LVDLKKELAE LKVQKLSRPS LPKIKTVRKS IACVLTVINE QQREAVRQLY KGKKYQPKDL RAKKTRALR RALTKFEASQ VTEKQRKKQI AFPQRKYAIK A

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Macromolecule #22: 60S ribosomal protein L36-A

MacromoleculeName: 60S ribosomal protein L36-A / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 11.151259 KDa
SequenceString:
MTVKTGIAIG LNKGKKVTSM TPAPKISYKK GAASNRTKFV RSLVREIAGL SPYERRLIDL IRNSGEKRAR KVAKKRLGSF TRAKAKVEE MNNIIAASRR H

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Macromolecule #23: 60S ribosomal protein L37-A

MacromoleculeName: 60S ribosomal protein L37-A / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 9.877395 KDa
SequenceString:
MGKGTPSFGK RHNKSHTLCN RCGRRSFHVQ KKTCSSCGYP AAKTRSYNWG AKAKRRHTTG TGRMRYLKHV SRRFKNGFQT GSASKASA

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Macromolecule #24: 60S ribosomal protein L38

MacromoleculeName: 60S ribosomal protein L38 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 8.845561 KDa
SequenceString:
MAREITDIKQ FLELTRRADV KTATVKINKK LNKAGKPFRQ TKFKVRGSSS LYTLVINDAG KAKKLIQSLP PTLKVNRL

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Macromolecule #25: 60S ribosomal protein L39

MacromoleculeName: 60S ribosomal protein L39 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 6.35864 KDa
SequenceString:
MAAQKSFRIK QKMAKAKKQN RPLPQWIRLR TNNTIRYNAK RRNWRRTKMN I

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Macromolecule #26: Ubiquitin-60S ribosomal protein L40

MacromoleculeName: Ubiquitin-60S ribosomal protein L40 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 14.583077 KDa
SequenceString:
MQIFVKTLTG KTITLEVESS DTIDNVKSKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGIIEP SLKALASKY NCDKSVCRKC YARLPPRATN CRKRKCGHTN QLRPKKKLK

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Macromolecule #27: 60S ribosomal protein L42-A

MacromoleculeName: 60S ribosomal protein L42-A / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 12.246658 KDa
SequenceString:
MVNVPKTRKT YCKGKTCRKH TQHKVTQYKA GKASLFAQGK RRYDRKQSGF GGQTKPVFHK KAKTTKKVVL RLECVKCKTR AQLTLKRCK HFELGGEKKQ KGQALQF

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Macromolecule #28: 60S ribosomal protein L43-A

MacromoleculeName: 60S ribosomal protein L43-A / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 10.112952 KDa
SequenceString:
MAKRTKKVGI TGKYGVRYGS SLRRQVKKLE IQQHARYDCS FCGKKTVKRG AAGIWTCSCC KKTVAGGAYT VSTAAAATVR STIRRLREM VEA

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Macromolecule #29: 60S ribosomal protein L41-A

MacromoleculeName: 60S ribosomal protein L41-A / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 3.354243 KDa
SequenceString:
MRAKWRKKRT RRLKRKRRKV RARSK

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Macromolecule #33: 60S ribosomal protein L2-A

MacromoleculeName: 60S ribosomal protein L2-A / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 27.463574 KDa
SequenceString: MGRVIRNQRK GAGSIFTSHT RLRQGAAKLR TLDYAERHGY IRGIVKQIVH DSGRGAPLAK VVFRDPYKYR LREEIFIANE GVHTGQFIY AGKKASLNVG NVLPLGSVPE GTIVSNVEEK PGDRGALARA SGNYVIIIGH NPDENKTRVR LPSGAKKVIS S DARGVIGV ...String:
MGRVIRNQRK GAGSIFTSHT RLRQGAAKLR TLDYAERHGY IRGIVKQIVH DSGRGAPLAK VVFRDPYKYR LREEIFIANE GVHTGQFIY AGKKASLNVG NVLPLGSVPE GTIVSNVEEK PGDRGALARA SGNYVIIIGH NPDENKTRVR LPSGAKKVIS S DARGVIGV IAGGGRVDKP LLKAGRAFHK YRLKRNSWPK TRGVAMNPVD HPHGGGNHQH IGKASTISRG AVSGQKAGLI AA RRTGLLR GSQKTQD

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Macromolecule #34: 60S ribosomal protein L3

MacromoleculeName: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 43.850793 KDa
SequenceString: MSHRKYEAPR HGHLGFLPRK RAASIRARVK AFPKDDRSKP VALTSFLGYK AGMTTIVRDL DRPGSKFHKR EVVEAVTVVD TPPVVVVGV VGYVETPRGL RSLTTVWAEH LSDEVKRRFY KNWYKSKKKA FTKYSAKYAQ DGAGIERELA RIKKYASVVR V LVHTQIRK ...String:
MSHRKYEAPR HGHLGFLPRK RAASIRARVK AFPKDDRSKP VALTSFLGYK AGMTTIVRDL DRPGSKFHKR EVVEAVTVVD TPPVVVVGV VGYVETPRGL RSLTTVWAEH LSDEVKRRFY KNWYKSKKKA FTKYSAKYAQ DGAGIERELA RIKKYASVVR V LVHTQIRK TPLAQKKAHL AEIQLNGGSI SEKVDWAREH FEKTVAVDSV FEQNEMIDAI AVTKGHGFEG VTHRWGTKKL PR KTHRGLR KVACIGAWHP AHVMWSVARA GQRGYHSRTS INHKIYRVGK GDDEANGATS FDRTKKTITP MGGFVHYGEI KND FIMVKG CIPGNRKRIV TLRKSLYTNT SRKALEEVSL KWIDTASKFG KGRFQTPAEK HAFMGTLKKD L

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Macromolecule #35: 60S ribosomal protein L4-A

MacromoleculeName: 60S ribosomal protein L4-A / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 39.159125 KDa
SequenceString: MSRPQVTVHS LTGEATANAL PLPAVFSAPI RPDIVHTVFT SVNKNKRQAY AVSEKAGHQT SAESWGTGRA VARIPRVGGG GTGRSGQGA FGNMCRGGRM FAPTKTWRKW NVKVNHNEKR YATASAIAAT AVASLVLARG HRVEKIPEIP LVVSTDLESI Q KTKEAVAA ...String:
MSRPQVTVHS LTGEATANAL PLPAVFSAPI RPDIVHTVFT SVNKNKRQAY AVSEKAGHQT SAESWGTGRA VARIPRVGGG GTGRSGQGA FGNMCRGGRM FAPTKTWRKW NVKVNHNEKR YATASAIAAT AVASLVLARG HRVEKIPEIP LVVSTDLESI Q KTKEAVAA LKAVGAHSDL LKVLKSKKLR AGKGKYRNRR WTQRRGPLVV YAEDNGIVKA LRNVPGVETA NVASLNLLQL AP GAHLGRF VIWTEAAFTK LDQVWGSETV ASSKVGYTLP SHIISTSDVT RIINSSEIQS AIRPAGQATQ KRTHVLKKNP LKN KQVLLR LNPYAKVFAA EKLGSKKAEK TGTKPAAVFT ETLKHD

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Macromolecule #36: 60S ribosomal protein L5

MacromoleculeName: 60S ribosomal protein L5 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 33.764828 KDa
SequenceString: MAFQKDAKSS AYSSRFQTPF RRRREGKTDY YQRKRLVTQH KAKYNTPKYR LVVRFTNKDI ICQIISSTIT GDVVLAAAYS HELPRYGIT HGLTNWAAAY ATGLLIARRT LQKLGLDETY KGVEEVEGEY ELTEAVEDGP RPFKVFLDIG LQRTTTGARV F GALKGASD ...String:
MAFQKDAKSS AYSSRFQTPF RRRREGKTDY YQRKRLVTQH KAKYNTPKYR LVVRFTNKDI ICQIISSTIT GDVVLAAAYS HELPRYGIT HGLTNWAAAY ATGLLIARRT LQKLGLDETY KGVEEVEGEY ELTEAVEDGP RPFKVFLDIG LQRTTTGARV F GALKGASD GGLYVPHSEN RFPGWDFETE EIDPELLRSY IFGGHVSQYM EELADDDEER FSELFKGYLA DDIDADSLED IY TSAHEAI RADPAFKPTE KKFTKEQYAA ESKKYRQTKL SKEERAARVA AKIAALAGQQ

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Macromolecule #37: 60S ribosomal protein L6-B

MacromoleculeName: 60S ribosomal protein L6-B / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 20.024541 KDa
SequenceString:
MTAQQAPKWY PSEDVAAPKK TRKAVRPQKL RASLVPGTVL ILLAGRFRGK RVVYLKHLED NTLLVTGPFK VNGVPLRRVN ARYVIATST KVSVEGVNVE KFNVEYFAKE KLTKKEKKEA NLFPEQQTKE IKTERVEDQK VVDKALLAEI KKTPLLKQYL S ASFSLKNG DKPHLLKF

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Macromolecule #38: 60S ribosomal protein L7-A

MacromoleculeName: 60S ribosomal protein L7-A / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 27.686281 KDa
SequenceString: MAAEKILTPE SQLKKSKAQQ KTAEQVAAER AARKAANKEK RAIILERNAA YQKEYETAER NIIQAKRDAK AAGSYYVEAQ HKLVFVVRI KGINKIPPKP RKVLQLLRLT RINSGTFVKV TKATLELLKL IEPYVAYGYP SYSTIRQLVY KRGFGKINKQ R VPLSDNAI ...String:
MAAEKILTPE SQLKKSKAQQ KTAEQVAAER AARKAANKEK RAIILERNAA YQKEYETAER NIIQAKRDAK AAGSYYVEAQ HKLVFVVRI KGINKIPPKP RKVLQLLRLT RINSGTFVKV TKATLELLKL IEPYVAYGYP SYSTIRQLVY KRGFGKINKQ R VPLSDNAI IEANLGKYGI LSIDDLIHEI ITVGPHFKQA NNFLWPFKLS NPSGGWGVPR KFKHFIQGGS FGNREEFINK LV KSMN

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Macromolecule #39: 60S ribosomal protein L8-A

MacromoleculeName: 60S ribosomal protein L8-A / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 28.17582 KDa
SequenceString: MAPGKKVAPA PFGAKSTKSN KTRNPLTHST PKNFGIGQAV QPKRNLSRYV KWPEYVRVQR QKKILSIRLK VPPTIAQFQY TLDRNTAAE TFKLFNKYRP ETAAEKKERL TKEAAAVAEG KSKQDASPKP YAVKYGLNHV VALIENKKAK LVLIANDVDP I ELVVFLPA ...String:
MAPGKKVAPA PFGAKSTKSN KTRNPLTHST PKNFGIGQAV QPKRNLSRYV KWPEYVRVQR QKKILSIRLK VPPTIAQFQY TLDRNTAAE TFKLFNKYRP ETAAEKKERL TKEAAAVAEG KSKQDASPKP YAVKYGLNHV VALIENKKAK LVLIANDVDP I ELVVFLPA LCKKMGVPYA IVKGKARLGT LVNQKTSAVA ALTEVRAEDE AALAKLVSTI DANFADKYDE VKKHWGGGIL GN KAQAKMD KRAKNSDSA

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Macromolecule #40: 60S ribosomal protein L9-A

MacromoleculeName: 60S ribosomal protein L9-A / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 21.605061 KDa
SequenceString:
MKYIQTEQQI EVPEGVTVSI KSRIVKVVGP RGTLTKNLKH IDVTFTKVNN QLIKVAVHNG GRKHVAALRT VKSLVDNMIT GVTKGYKYK MRYVYAHFPI NVNIVEKDGA KFIEVRNFLG DKKIRNVPVR DGVTIEFSTN VKDEIVLSGN SVEDVSQNAA D LQQICRVR NKDIRKFLDG IYVSHKGFIT EDL

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Macromolecule #41: 60S ribosomal protein L10

MacromoleculeName: 60S ribosomal protein L10 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 25.410465 KDa
SequenceString: MARRPARCYR YQKNKPYPKS RYNRAVPDSK IRIYDLGKKK ATVDEFPLCV HLVSNELEQL SSEALEAARI CANKYMTTVS GRDAFHLRV RVHPFHVLRI NKMLSCAGAD RLQQGMRGAW GKPHGLAARV DIGQIIFSVR TKDSNKDVVV EGLRRARYKF P GQQKIILS ...String:
MARRPARCYR YQKNKPYPKS RYNRAVPDSK IRIYDLGKKK ATVDEFPLCV HLVSNELEQL SSEALEAARI CANKYMTTVS GRDAFHLRV RVHPFHVLRI NKMLSCAGAD RLQQGMRGAW GKPHGLAARV DIGQIIFSVR TKDSNKDVVV EGLRRARYKF P GQQKIILS KKWGFTNLDR PEYLKKREAG EVKDDGAFVK FLSKKGSLEN NIREFPEYFA AQA

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Macromolecule #42: 60S ribosomal protein L11-A

MacromoleculeName: 60S ribosomal protein L11-A / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 19.755691 KDa
SequenceString:
MSAKAQNPMR DLKIEKLVLN ISVGESGDRL TRASKVLEQL SGQTPVQSKA RYTVRTFGIR RNEKIAVHVT VRGPKAEEIL ERGLKVKEY QLRDRNFSAT GNFGFGIDEH IDLGIKYDPS IGIFGMDFYV VMNRPGARVT RRKRCKGTVG NSHKTTKEDT V SWFKQKYD ADVLDK

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Macromolecule #43: 60S ribosomal protein L13-A

MacromoleculeName: 60S ribosomal protein L13-A / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 22.604164 KDa
SequenceString: MAISKNLPIL KNHFRKHWQE RVKVHFDQAG KKVSRRNARA TRAAKIAPRP LDLLRPVVRA PTVKYNRKVR AGRGFTLAEV KAAGLTAAY ARTIGIAVDH RRQNRNQEIF DANVQRLKEY QSKIIVFPRN GKAPEAEQVL SAAATFPIAQ PATDVEARAV Q DNGESAFR ...String:
MAISKNLPIL KNHFRKHWQE RVKVHFDQAG KKVSRRNARA TRAAKIAPRP LDLLRPVVRA PTVKYNRKVR AGRGFTLAEV KAAGLTAAY ARTIGIAVDH RRQNRNQEIF DANVQRLKEY QSKIIVFPRN GKAPEAEQVL SAAATFPIAQ PATDVEARAV Q DNGESAFR TLRLARSEKK FRGIREKRAR EKAEAEAEKK K

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Macromolecule #44: 60S ribosomal protein L14-A

MacromoleculeName: 60S ribosomal protein L14-A / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 15.195066 KDa
SequenceString:
MSTDSIVKAS NWRLVEVGRV VLIKKGQSAG KLAAIVEIID QKKVLIDGPK AGVPRQAINL GQVVLTPLTF ALPRGARTAT VSKKWAAAA VCEKWAASSW AKKIAQRERR AALTDFERFQ VMVLRKQKRY TVKKALAKA

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Macromolecule #45: RQC2 isoform 1

MacromoleculeName: RQC2 isoform 1 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 119.250234 KDa
SequenceString: MKQRISALDL LLLARELKQD LEGYRLSNIY NIADSSKQFL LKFNKPDSKL NVVVDCGLRI YLTEFSRPIP PTPSGFVVKL RKHLKAKRL TALKQVDQDR ILVLQFADGH FYLVLEFFSA GNVILLDENR RIMALQRVVL EHENKVGQIY EMFDESLFTT N NESADESI ...String:
MKQRISALDL LLLARELKQD LEGYRLSNIY NIADSSKQFL LKFNKPDSKL NVVVDCGLRI YLTEFSRPIP PTPSGFVVKL RKHLKAKRL TALKQVDQDR ILVLQFADGH FYLVLEFFSA GNVILLDENR RIMALQRVVL EHENKVGQIY EMFDESLFTT N NESADESI EKNRKAEYTS ELVNEWIKAV QAKYESDITV IKQLNIQGKE GAKKKKVKVP SIHKLLLSKV PHLSSDLLSK NL KVFNIDP SESCLNLLEE TDSLAELLNS TQLEYNQLLT TTDRKGYILA KRNENYISEK DTADLEFIYD TFHPFKPYIN GGD TDSSCI IEVEGPYNRT LDKFFSTIES SKYALRIQNQ ESQAQKKIDD ARAENDRKIQ ALLDVQELNE RKGHLIIENA PLIE EVKLA VQGLIDQQMD WNTIEKLIKS EQKKGNRIAQ LLNLPLNLKQ NKISVKLDLS SKELNTSSDE DNESEGNTTD SSSDS DSED MESSKERSTK SMKRKSNEKI NVTIDLGLSA YANATEYFNI KKTSAQKQKK VEKNVGKAMK NIEVKIDQQL KKKLKD SHS VLKKIRTPYF FEKYSWFISS EGFLVMMGKS PAETDQIYSK YIEDDDIYMS NSFNSHVWIK NPEKTEVPPN TLMQAGI LC MSSSEAWSKK ISSSPWWCFA KNVSKFDGSD NSILPEGAFR LKNENDQNHL PPAQLVMGFG FLWKVKTSGN EDNGDDDE E EEEEEEEEEE EEEEEEEEEE EEKEEEEKEE EQQQDEDDSN EVNGLEKGGD SNDSTKNNSF EHDNLEKDIE KHCTISSDT DSDSGNAKAK NDNSSTQRIL DEPGVPISLI ENINSNVRGK RGKLKKIQKK YADQDETERL LRLEALGTLK GIEKQQQRKK EEIMKREVR EDRKNKREKQ RRLQALKFTK KEKARVNYDK HKSELKPSLD KGDVVDDIIP VFAPWPALLK YKYKVKIQPG S AKKTKTLT EILHYFKSRP LDGSSTDNEM DWPQEHEMIK GLKEQDLVLL LCVDKLKVTI AGQKSTKNGG NSSKKGKKKR

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Macromolecule #46: E3 ubiquitin-protein ligase listerin

MacromoleculeName: E3 ubiquitin-protein ligase listerin / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO / EC number: RING-type E3 ubiquitin transferase
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 180.372984 KDa
SequenceString: MSFGGINTFQ QYNTDLGLGH NGVRISLNYF DGLPDPSLLN SLYSNELKLI FKSLLKRDET TKEKALMDLS NLISDFNQNE YFFNDIFLL CWSQIYAKLI ISDYKVIRLQ SHQITIMLVK SLRKKISKFL KDFIPLILLG TCELDYSVSK PSLNELTECF N KDPAKINA ...String:
MSFGGINTFQ QYNTDLGLGH NGVRISLNYF DGLPDPSLLN SLYSNELKLI FKSLLKRDET TKEKALMDLS NLISDFNQNE YFFNDIFLL CWSQIYAKLI ISDYKVIRLQ SHQITIMLVK SLRKKISKFL KDFIPLILLG TCELDYSVSK PSLNELTECF N KDPAKINA LWAVFQEQLL NLVKEIVVNE NEDTISDERY SSKEESEFRY HRVIASAVLL LIKLFVHNKD VSERNSSSLK VI LSDESIW KLLNLKNGQN TNAYETVLRL IDVLYTRGYM PSHKNIMKLA VKKLLKSLTH ITSKNILKVC PVLPSILNLL ATL DDYEDG TIWSYDKSSK EKVLKFLSVS RTSPSPGFFN AVFALYSSTK RHSFLDYYLE WLPFWQKSVQ RLNEKGFSAR NSAE VLNEF WTNFLKFAED SSEERVKKMV ESEIFNSLSC GKSLSEYTKL NQTLSGVFPP DKWEREIEDY FTSDEDIRKI KVSFE KNLF ALLVTSPNNE SAISRLFDFF VQLIETDPSN VFNKYDGVYD ALNYFLDSDM IFLNGKIGKF INEIPTLVQE STYQNF AGI MAQYSNSKFF KMNTDAITSL EDFFIVALSF NLPKTIILAT MNELDNDIYQ QLMKSDSLEL ELYIEDFMKN YKFDDSG EI FKGNNKFLNQ RTITTLYRSA VANGQVEQFC AVLSKLDETF FSTLLLNTDF LSCALYEVSE DTNEKLFKLS LQLAKGNS E IANKLAQVIL QHAQVYFSPG AKEKYVTHAV ELINGCNDTS QIFFPANAIE VFARYMPAID YRSSLVSSLS TNTHLLLTD DKPINLKNMQ KLIRYALFLD ALLDALPERV NNHIVAFITV VSELVTDYNC LSEEPNDLYY DFGHTFFKHG KVNLNFSDIV GNVIQPANG GDAMLTFDIA ESNSVYFFYY SRVLYKVLLN SIDTVSSTTL NGLLASVESF VTKTVRDQKS TDKDYLLCAI L LLMFNRSN SKDEITKLRT LLASQLIGIR EVELVDQEFK SLALLNNLLD IPQADKQFVP IAPQRLNMIF RSILKWLDSD LA YEPSFST VRLLLLDFFT KLMRFEGVRD MGITAFELSE RLLADSLSMC QIDDTLYLLE LRSSCLNLYE TLSQGVSKNG EEI SEYGDE IQENLIELMF LNFNQERNNQ VSTLFYQKLY KVISSMELKK LESQYKRIFE VVLNDKDIGS NINQSRLLTT LLGS LVVKT QQDIIIEYEL RIQKQTGSDV DGSASDNDVN SKFKLPQKLL QKVTDEVPKE YLEYENKNSF IKYLWYWHLI LMYFK DTSY NMRQIFIEQL KEAGLINRMF DFITDQIDLR DTEFWKQVDT KEISEYNIVG NNFSPYKEDI FEECKKLLGH TLYQLF NNV GCLTSIWWLN IKDRTLQNDI EKFVSEFISP ILIKNEFDDI NSKMDRLTSN DDALTIKLNN ITNEVKASYL IDDQKLE IS FKLPKNYPLT NIQVNGVSRV GISEQKWKQW IMSTQHVITG MNGSVLDSLE LFTKNVHLQF SGFEECAICY SILHAVDR K LPSKTCPTCK NKFHGACLYK WFRSSGNNTC PLCRSEIPFR R

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Macromolecule #47: Eukaryotic translation initiation factor 6

MacromoleculeName: Eukaryotic translation initiation factor 6 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 26.476605 KDa
SequenceString: MATRTQFENS NEIGVFSKLT NTYCLVAVGG SENFYSAFEA ELGDAIPIVH TTIAGTRIIG RMTAGNRRGL LVPTQTTDQE LQHLRNSLP DSVKIQRVEE RLSALGNVIC CNDYVALVHP DIDRETEELI SDVLGVEVFR QTISGNILVG SYCSLSNQGG L VHPQTSVQ ...String:
MATRTQFENS NEIGVFSKLT NTYCLVAVGG SENFYSAFEA ELGDAIPIVH TTIAGTRIIG RMTAGNRRGL LVPTQTTDQE LQHLRNSLP DSVKIQRVEE RLSALGNVIC CNDYVALVHP DIDRETEELI SDVLGVEVFR QTISGNILVG SYCSLSNQGG L VHPQTSVQ DQEELSSLLQ VPLVAGTVNR GSSVVGAGMV VNDYLAVTGL DTTAPELSVI ESIFRLQDAQ PESISGNLRD TL IETYS

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Macromolecule #49: 60S ribosomal protein L12-A

MacromoleculeName: 60S ribosomal protein L12-A / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 17.850621 KDa
SequenceString:
MPPKFDPNEV KYLYLRAVGG EVGASAALAP KIGPLGLSPK KVGEDIAKAT KEFKGIKVTV QLKIQNRQAA ASVVPSASSL VITALKEPP RDRKKDKNVK HSGNIQLDEI IEIARQMRDK SFGRTLASVT KEILGTAQSV GCRVDFKNPH DIIEGINAGE I EIPEN

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Macromolecule #50: 60S acidic ribosomal protein P0

MacromoleculeName: 60S acidic ribosomal protein P0 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 33.749121 KDa
SequenceString: MGGIREKKAE YFAKLREYLE EYKSLFVVGV DNVSSQQMHE VRKELRGRAV VLMGKNTMVR RAIRGFLSDL PDFEKLLPFV KGNVGFVFT NEPLTEIKNV IVSNRVAAPA RAGAVAPEDI WVRAVNTGME PGKTSFFQAL GVPTKIARGT IEIVSDVKVV D AGNKVGQS ...String:
MGGIREKKAE YFAKLREYLE EYKSLFVVGV DNVSSQQMHE VRKELRGRAV VLMGKNTMVR RAIRGFLSDL PDFEKLLPFV KGNVGFVFT NEPLTEIKNV IVSNRVAAPA RAGAVAPEDI WVRAVNTGME PGKTSFFQAL GVPTKIARGT IEIVSDVKVV D AGNKVGQS EASLLNLLNI SPFTFGLTVV QVYDNGQVFP SSILDITDEE LVSHFVSAVS TIASISLAIG YPTLPSVGHT LI NNYKDLL AVAIAASYHY PEIEDLVDRI ENPEKYAAAA PAATSAASGD AAPAEEAAAE EEEESDDDMG FGLFD

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Macromolecule #51: CAT-tailed nascent peptide

MacromoleculeName: CAT-tailed nascent peptide / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 1.464797 KDa
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

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Macromolecule #52: 60S ribosomal protein L1-A

MacromoleculeName: 60S ribosomal protein L1-A / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 24.524799 KDa
SequenceString: MSKITSSQVR EHVKELLKYS NETKKRNFLE TVELQVGLKN YDPQRDKRFS GSLKLPNCPR PNMSICIFGD AFDVDRAKSC GVDAMSVDD LKKLNKNKKL IKKLSKKYNA FIASEVLIKQ VPRLLGPQLS KAGKFPTPVS HNDDLYGKVT DVRSTIKFQL K KVLCLAVA ...String:
MSKITSSQVR EHVKELLKYS NETKKRNFLE TVELQVGLKN YDPQRDKRFS GSLKLPNCPR PNMSICIFGD AFDVDRAKSC GVDAMSVDD LKKLNKNKKL IKKLSKKYNA FIASEVLIKQ VPRLLGPQLS KAGKFPTPVS HNDDLYGKVT DVRSTIKFQL K KVLCLAVA VGNVEMEEDV LVNQILMSVN FFVSLLKKNW QNVGSLVVKS SMGPAFRLY

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Macromolecule #30: 25S rRNA

MacromoleculeName: 25S rRNA / type: rna / ID: 30 / Number of copies: 1
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 1.0970864999999999 MDa
SequenceString: GUUUGACCUC AAAUCAGGUA GGAGUACCCG CUGAACUUAA GCAUAUCAAU AAGCGGAGGA AAAGAAACCA ACCGGGAUUG CCUUAGUAA CGGCGAGUGA AGCGGCAAAA GCUCAAAUUU GAAAUCUGGU ACCUUCGGUG CCCGAGUUGU AAUUUGGAGA G GGCAACUU ...String:
GUUUGACCUC AAAUCAGGUA GGAGUACCCG CUGAACUUAA GCAUAUCAAU AAGCGGAGGA AAAGAAACCA ACCGGGAUUG CCUUAGUAA CGGCGAGUGA AGCGGCAAAA GCUCAAAUUU GAAAUCUGGU ACCUUCGGUG CCCGAGUUGU AAUUUGGAGA G GGCAACUU UGGGGCCGUU CCUUGUCUAU GUUCCUUGGA ACAGGACGUC AUAGAGGGUG AGAAUCCCGU GUGGCGAGGA GU GCGGUUC UUUGUAAAGU GCCUUCGAAG AGUCGAGUUG UUUGGGAAUG CAGCUCUAAG UGGGUGGUAA AUUCCAUCUA AAG CUAAAU AUUGGCGAGA GACCGAUAGC GAACAAGUAC AGUGAUGGAA AGAUGAAAAG AACUUUGAAA AGAGAGUGAA AAAG UACGU GAAAUUGUUG AAAGGGAAGG GCAUUUGAUC AGACAUGGUG UUUUGUGCCC UCUGCUCCUU GUGGGUAGGG GAAUC UCGC AUUUCACUGG CCAGCAUCAG UUUUGGUGGC AGGAUAAAUC CAUAGGAAUG UAGCUUGCCU CGGUAAGUAU UAUAGC CUG UGGGAAUACU GCCAGCUGGG ACUGAGGACU GCGACGUAAG UCAAGGAUGC UGGCAUAAUG GUUAUAUGCC GCCCGUC UU GAAACACGGA CCAAGGAGUC UAACGUCUAU GCGAGUGUUU GGGUGUAAAA CCCAUACGCG UAAUGAAAGU GAACGUAG G UUGGGGCCUC GCAAGAGGUG CACAAUCGAC CGAUCCUGAU GUCUUCGGAU GGAUUUGAGU AAGAGCAUAG CUGUUGGGA CCCGAAAGAU GGUGAACUAU GCCUGAAUAG GGUGAAGCCA GAGGAAACUC UGGUGGAGGC UCGUAGCGGU UCUGACGUGC AAAUCGAUC GUCGAAUUUG GGUAUAGGGG CGAAAGACUA AUCGAACCAU CUAGUAGCUG GUUCCUGCCG AAGUUUCCCU C AGGAUAGC AGAAGCUCGU AUCAGUUUUA UGAGGUAAAG CGAAUGAUUA GAGGUUCCGG GGUCGAAAUG ACCUUGACCU AU UCUCAAA CUUUAAAUAU GUAAGAAGUC CUUGUUACUU AAUUGAACGU GGACAUUUGA AUGAAGAGCU UUUAGUGGGC CAU UUUUGG UAAGCAGAAC UGGCGAUGCG GGAUGAACCG AACGUAGAGU UAAGGUGCCG GAAUACACGC UCAUCAGACA CCAC AAAAG GUGUUAGUUC AUCUAGACAG CCGGACGGUG GCCAUGGAAG UCGGAAUCCG CUAAGGAGUG UGUAACAACU CACCG GCCG AAUGAACUAG CCCUGAAAAU GGAUGGCGCU CAAGCGUGUU ACCUAUACUC UACCGUCAGG GUUGAUAUGA UGCCCU GAC GAGUAGGCAG GCGUGGAGGU CAGUGACGAA GCCUAGACCG UAAGGUCGGG UCGAACGGCC UCUAGUGCAG AUCUUGG UG GUAGUAGCAA AUAUUCAAAU GAGAACUUUG AAGACUGAAG UGGGGAAAGG UUCCACGUCA ACAGCAGUUG GACGUGGG U UAGUCGAUCC UAAGAGAUGG GGAAGCUCCG UUUCAAAGGC CUGAUUUUAU GCAGGCCACC AUCGAAAGGG AAUCCGGUU AAGAUUCCGG AACCUGGAUA UGGAUUCUUC ACGGUAACGU AACUGAAUGU GGAGACGUCG GCGCGAGCCC UGGGAGGAGU UAUCUUUUC UUCUUAACAG CUUAUCACCC CGGAAUUGGU UUAUCCGGAG AUGGGGUCUU AUGGCUGGAA GAGGCCAGCA C CUUUGCUG GCUCCGGUGC GCUUGUGACG GCCCGUGAAA AUCCACAGGA AGGAAUAGUU UUCAUGCCAG GUCGUACUGA UA ACCGCAG CAGGUCUCCA AGGUGAACAG CCUCUAGUUG AUAGAAUAAU GUAGAUAAGG GAAGUCGGCA AAAUAGAUCC GUA ACUUCG GGAUAAGGAU UGGCUCUAAG GGUCGGGUUA GUGAGGGCCU UGGUCAGACG CAGCGGGCGU GCUUGUGGAC UGCU UGGUG GGGCUUGCUC UGCUAGGCGG ACUACUUGCG UGCCUUGUUG UAGACGGCCU UGGUAGGUCU CUUGUAGACC GUCGC UUGC UACAAUUACG AUCAACUUAG AACUGGUACG GACAAGGGGA AUCUGACUGU CUAAUUAAAA CAUAGCAUUG CGAUGG UCA GAAAGUGAUG UUGACGCAAU GUGAUUUCUG CCCAGUGCUC UGAAUGUCAA AGUGAAGAAA UUCAACCAAG CGCGGGU AA ACGGCGGGAG UUAACUAUAC UCUCUUAAGG UAGCCAAAUG CCUCGUCAUC UAAUUAGUGA CGCGCAUGAA UGGAUUAA C GAGAUUCCCA CUGUCCCUAU CUACUAUCUA GCGAAACCAC AGCCAAGGGA ACGGGCUUGG CAGAAUCAGC GGGGAAAGA AGACCCUGUU GAGCUUGACU CUAGUUUGAC AUUGUGAAGA GACAUAGAGG GUGUAGAAUA AGUGGGAGCU UCGGCGCCAG UGAAAUACC ACUACCUUUA UAGUUUCUUU ACUUAUUCAA UGAAGCGGAG CUGGAAUUCA UUUUCCACGU UCUAGCAUUC A AGGUCCCA UUCGGGGCUG AUCCGGGUUG AAGACAUUGU CAGGUGGGGA GUUUGGCUGG GGCGGCACAU CUGUUAAACG AU AACGCAG AUGUCCUAAG GGGGGCUCAU GGAGAACAGA AAUCUCCAGU AGAACAAAAG GGUAAAAGCC CCCUUGAUUU UGA UUUUCA GUGUGAAUAC AAACCAUGAA AGUGUGGCCU AUCGAUCCUU UAGUCCCUCG GAAUUUGAGG CUAGAGGUGC CAGA AAAGU UACCACAGGG AUAACUGGCU UGUGGCAGUC AAGCGUUCAU AGCGACAUUG CUUUUUGAUU CUUCGAUGUC GGCUC UUCC UAUCAUACCG AAGCAGAAUU CGGUAAGCGU UGGAUUGUUC ACCCACUAAU AGGGAACGUG AGCUGGGUUU AGACCG UCG UGAGACAGGU UAGUUUUACC CUACUGAUGA AUGUUACCGC AAUAGUAAUU GAACUUAGUA CGAGAGGAAC AGUUCAU UC GGAUAAUUGG UUUUUGCGGC UGUCUGAUCA GGCAUUGCCG CGAAGCUACC AUCCGCUGGA UUAUGGCUGA ACGCCUCU A AGUCAGAAUC CAUGCUAGAA CGCGGUGAUU UCUUUGCUCC ACACAAUAUA GAUGGAUACG AAUAAGGCGU CCUUGUGGC GUCGCUGAAC CAUAGCAGGC UAGCAACGGU GCACUUGGCG GAAAGGCCUU GGGUGCUUGC UGGCGAAUUG CAAUGUCAUU UUGCGUGGG GAUAAAUCAU UUGUAUACGA CUUAGAUGUA CAACGGGGUA UUGUAAGCAG UAGAGUAGCC UUGUUGUUAC G AUCUGCUG AGAUUAAGCC UUUGUUGUCU GAUUUGU

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Macromolecule #31: 5S rRNA

MacromoleculeName: 5S rRNA / type: rna / ID: 31 / Number of copies: 1
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 38.951105 KDa
SequenceString:
GGUUGCGGCC AUAUCUACCA GAAAGCACCG UUUCCCGUCC GAUCAACUGU AGUUAAGCUG GUAAGAGCCU GACCGAGUAG UGUAGUGGG UGACCAUACG CGAAACUCAG GUGCUGCAAU CU

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Macromolecule #32: 5.8S rRNA

MacromoleculeName: 5.8S rRNA / type: rna / ID: 32 / Number of copies: 1
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 50.682922 KDa
SequenceString:
AAACUUUCAA CAACGGAUCU CUUGGUUCUC GCAUCGAUGA AGAACGCAGC GAAAUGCGAU ACGUAAUGUG AAUUGCAGAA UUCCGUGAA UCAUCGAAUC UUUGAACGCA CAUUGCGCCC CUUGGUAUUC CAGGGGGCAU GCCUGUUUGA GCGUCAUUU

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Macromolecule #48: Ala tRNA

MacromoleculeName: Ala tRNA / type: rna / ID: 48 / Number of copies: 2
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 24.502477 KDa
SequenceString:
GGGCGUGUGG CGUAGUCGGU AGCGCGCUCC CUUAGCAUGG GAGAGGUCUC CGGUUCGAUU CCGGACUCGU CCGCCA

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Macromolecule #53: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 53 / Number of copies: 13 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #54: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 54 / Number of copies: 7 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #55: SPERMIDINE

MacromoleculeName: SPERMIDINE / type: ligand / ID: 55 / Number of copies: 1 / Formula: SPD
Molecular weightTheoretical: 145.246 Da
Chemical component information

ChemComp-SPD:
SPERMIDINE / Spermidine

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 6.8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 4.0 µm / Nominal defocus min: 0.4 µm
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 46.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

6tnu

Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 58513
FSC plot (resolution estimation)

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