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Yorodumi- EMDB-15351: Pol alpha local refinement. Human replisome bound by Pol Alpha, e... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-15351 | |||||||||
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Title | Pol alpha local refinement. Human replisome bound by Pol Alpha, engaged on a fork DNA substrate containing a 60 nt lagging strand. | |||||||||
Map data | Local refinement of Pol Alpha. Human replisome bound by Pol Alpha, engaged on a fork DNA substrate containing a 60 nt lagging strand. | |||||||||
Sample |
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Keywords | Replication / helicase / polymerase / pol alpha / priming | |||||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.3 Å | |||||||||
Authors | Jones ML / Yeeles JTP | |||||||||
Funding support | United Kingdom, 1 items
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Citation | Journal: Mol.Cell / Year: 2023 Title: How Pol a-primase is targeted to replisomes to prime eukaryotic DNA replication Authors: Jones ML / Aria V / Baris Y / Yeeles JTP | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_15351.map.gz | 62.3 MB | EMDB map data format | |
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Header (meta data) | emd-15351-v30.xml emd-15351.xml | 11.5 KB 11.5 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_15351_fsc.xml | 10.6 KB | Display | FSC data file |
Images | emd_15351.png | 57.3 KB | ||
Others | emd_15351_half_map_1.map.gz emd_15351_half_map_2.map.gz | 115.9 MB 115.9 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-15351 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-15351 | HTTPS FTP |
-Validation report
Summary document | emd_15351_validation.pdf.gz | 792.5 KB | Display | EMDB validaton report |
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Full document | emd_15351_full_validation.pdf.gz | 792.1 KB | Display | |
Data in XML | emd_15351_validation.xml.gz | 19.1 KB | Display | |
Data in CIF | emd_15351_validation.cif.gz | 24.5 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-15351 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-15351 | HTTPS FTP |
-Related structure data
Related structure data |
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-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_15351.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Annotation | Local refinement of Pol Alpha. Human replisome bound by Pol Alpha, engaged on a fork DNA substrate containing a 60 nt lagging strand. | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.2363 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: Half map B
File | emd_15351_half_map_1.map | ||||||||||||
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Annotation | Half map B | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half map A
File | emd_15351_half_map_2.map | ||||||||||||
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Annotation | Half map A | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Replisome - pol alpha complex
Entire | Name: Replisome - pol alpha complex |
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Components |
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-Supramolecule #1: Replisome - pol alpha complex
Supramolecule | Name: Replisome - pol alpha complex / type: complex / ID: 1 / Parent: 0 Details: S. cerevisiae pol alpha bound to the core replisome engaged with a fork DNA substrate containing a 60 nucleotide lagging strand. |
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Source (natural) | Organism: Saccharomyces cerevisiae (brewer's yeast) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.6 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 40.184 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.5 µm / Nominal defocus min: 1.5 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |