+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-14734 | |||||||||
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Title | Tomogram of human sperm singlet microtubules | |||||||||
Map data | ||||||||||
Sample |
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Keywords | Microtubule Sperm TAILS / UNKNOWN FUNCTION | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | electron tomography / cryo EM | |||||||||
Authors | Hoog JL / Zabeo DZ | |||||||||
Funding support | Sweden, 1 items
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Citation | Journal: Sci Rep / Year: 2018 Title: A lumenal interrupted helix in human sperm tail microtubules. Authors: Davide Zabeo / John M Heumann / Cindi L Schwartz / Azusa Suzuki-Shinjo / Garry Morgan / Per O Widlund / Johanna L Höög / Abstract: Eukaryotic flagella are complex cellular extensions involved in many human diseases gathered under the term ciliopathies. Currently, detailed insights on flagellar structure come mostly from studies ...Eukaryotic flagella are complex cellular extensions involved in many human diseases gathered under the term ciliopathies. Currently, detailed insights on flagellar structure come mostly from studies on protists. Here, cryo-electron tomography (cryo-ET) was performed on intact human spermatozoon tails and showed a variable number of microtubules in the singlet region (inside the end-piece). Inside the microtubule plus end, a novel left-handed interrupted helix which extends several micrometers was discovered. This structure was named Tail Axoneme Intra-Lumenal Spiral (TAILS) and binds directly to 11 protofilaments on the internal microtubule wall, in a coaxial fashion with the surrounding microtubule lattice. It leaves a gap over the microtubule seam, which was directly visualized in both singlet and doublet microtubules. We speculate that TAILS may stabilize microtubules, enable rapid swimming or play a role in controlling the swimming direction of spermatozoa. | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_14734.map.gz | 199.9 MB | EMDB map data format | |
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Header (meta data) | emd-14734-v30.xml emd-14734.xml | 6.9 KB 6.9 KB | Display Display | EMDB header |
Images | emd_14734.png | 101.5 KB | ||
Filedesc metadata | emd-14734.cif.gz | 3.4 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-14734 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-14734 | HTTPS FTP |
-Validation report
Summary document | emd_14734_validation.pdf.gz | 470.4 KB | Display | EMDB validaton report |
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Full document | emd_14734_full_validation.pdf.gz | 469.9 KB | Display | |
Data in XML | emd_14734_validation.xml.gz | 5.1 KB | Display | |
Data in CIF | emd_14734_validation.cif.gz | 6.1 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-14734 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-14734 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_14734.map.gz / Format: CCP4 / Size: 230.6 MB / Type: IMAGE STORED AS SIGNED BYTE | ||||||||||||||||||||
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Voxel size | X=Y=Z: 7.7 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Sample components
-Entire : Human sperm flagellum end piece
Entire | Name: Human sperm flagellum end piece |
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Components |
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-Supramolecule #1: Human sperm flagellum end piece
Supramolecule | Name: Human sperm flagellum end piece / type: organelle_or_cellular_component / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Homo sapiens (human) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | electron tomography |
Aggregation state | cell |
-Sample preparation
Buffer | pH: 7 |
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Vitrification | Cryogen name: ETHANE |
Details | Ejaculate applied directly to EM grid after collection. |
Sectioning | Other: NO SECTIONING |
-Electron microscopy
Microscope | FEI TECNAI F30 |
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Image recording | Film or detector model: GATAN ULTRASCAN 4000 (4k x 4k) / Average electron dose: 1.23 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 6.0 µm / Nominal defocus min: 4.0 µm |
Experimental equipment | Model: Tecnai F30 / Image courtesy: FEI Company |
-Image processing
Final reconstruction | Number images used: 81 |
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