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- EMDB-14688: Complex I from Ovis aries at pH9, Closed state -

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Basic information

Entry
Database: EMDB / ID: EMD-14688
TitleComplex I from Ovis aries at pH9, Closed state
Map datacomposite map
Sample
  • Complex: Complex I from Ovis aries at pH9, Closed state
    • Protein or peptide: x 44 types
  • Ligand: x 11 types
Function / homology
Function and homology information


oxidoreductase activity, acting on NAD(P)H / NADH:ubiquinone reductase (H+-translocating) / apoptotic mitochondrial changes / NADH dehydrogenase activity / : / mitochondrial respiratory chain complex I assembly / mitochondrial electron transport, NADH to ubiquinone / electron transport coupled proton transport / acyl binding / acyl carrier activity ...oxidoreductase activity, acting on NAD(P)H / NADH:ubiquinone reductase (H+-translocating) / apoptotic mitochondrial changes / NADH dehydrogenase activity / : / mitochondrial respiratory chain complex I assembly / mitochondrial electron transport, NADH to ubiquinone / electron transport coupled proton transport / acyl binding / acyl carrier activity / NADH dehydrogenase (ubiquinone) activity / ATP synthesis coupled electron transport / quinone binding / membrane => GO:0016020 / : / ATP metabolic process / respiratory electron transport chain / reactive oxygen species metabolic process / FAD binding / regulation of mitochondrial membrane potential / electron transport chain / mitochondrial intermembrane space / 2 iron, 2 sulfur cluster binding / circadian rhythm / NAD binding / FMN binding / 4 iron, 4 sulfur cluster binding / response to oxidative stress / mitochondrial inner membrane / oxidoreductase activity / mitochondrial matrix / protein-containing complex binding / nucleus / metal ion binding
Similarity search - Function
Akirin / Activator of Hsp90 ATPase homologue 1-like / Activator of Hsp90 ATPase homolog 1-like protein / : / NADH-ubiquinone oxidoreductase 1 subunit C1 / NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / FAD-binding domain / NADH:ubiquinone oxidoreductase, ESSS subunit / ESSS subunit of NADH:ubiquinone oxidoreductase (complex I) / FAD binding domain ...Akirin / Activator of Hsp90 ATPase homologue 1-like / Activator of Hsp90 ATPase homolog 1-like protein / : / NADH-ubiquinone oxidoreductase 1 subunit C1 / NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / FAD-binding domain / NADH:ubiquinone oxidoreductase, ESSS subunit / ESSS subunit of NADH:ubiquinone oxidoreductase (complex I) / FAD binding domain / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1, NDUB1 / MNLL subunit / NADH-ubiquinone oxidoreductase flavoprotein 3 / NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial / NADH-ubiquinone oxidoreductase, subunit 10 / NADH-ubiquinone oxidoreductase subunit 10 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, metazoa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, animal type / NADH dehydrogenase 1 beta subcomplex subunit 2 / NADH dehydrogenase [ubiquinone] 1 subunit C2, NDUC2 / NADH-ubiquinone oxidoreductase subunit b14.5b (NDUFC2) / NADH dehydrogenase 1, beta subcomplex, subunit 6 / NADH:ubiquinone oxidoreductase, NDUFB6/B17 subunit / NADH:ubiquinone oxidoreductase chain 4, N-terminal / NADH:ubiquinone oxidoreductase, chain 2 / NADH dehydrogenase subunit 2, C-terminal / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial / NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit / : / NADH-ubiquinone oxidoreductase chain 4, amino terminus / NADH dehydrogenase subunit 2 C-terminus / NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex subunit 1 / NADH-ubiquinone oxidoreductase MWFE subunit / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 / NADH-ubiquinone oxidoreductase ASHI subunit (CI-ASHI or NDUFB8) / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 / NADH:ubiquinone oxidoreductase subunit B14.5a (Complex I-B14.5a) / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 / NADH dehydrogenase 1 alpha subcomplex subunit 3 / Zinc finger, CHCC-type / Zinc-finger domain / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / NADH dehydrogenase subunit 5, C-terminal / NADH dehydrogenase subunit 5 C-terminus / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH-ubiquinone oxidoreductase B12 subunit family / NADH:ubiquinone oxidoreductase, NDUFS5-15kDa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7, NDUB7 / NADH-ubiquinone oxidoreductase B18 subunit (NDUFB7) / GRIM-19 / GRIM-19 protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NDUFB9, LYR domain / NADH:ubiquinone oxidoreductase, iron-sulphur subunit 5 / NDUFA6, LYR domain / NADH dehydrogenase ubiquinone Fe-S protein 4-like superfamily / NADH dehydrogenase ubiquinone Fe-S protein 4 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 2 / Deoxynucleoside kinase domain / Deoxynucleoside kinase / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrial / START-like domain superfamily / ETC complex I subunit conserved region / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH ubiquinone oxidoreductase subunit NDUFA12 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 6 / NADH-quinone oxidoreductase, chain G, C-terminal / NADH-ubiquinone oxidoreductase subunit G, C-terminal / SLBB domain / : / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 8 / Respiratory-chain NADH dehydrogenase 20 Kd subunit signature. / NADH-ubiquinone oxidoreductase, 20 Kd subunit / NADH-quinone oxidoreductase, chain I / NADH-plastoquinone oxidoreductase, chain 5 subgroup / NADH-quinone oxidoreductase, chain M/4 / NADH-ubiquinone oxidoreductase chain 4L/K / Complex 1 LYR protein domain / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminal / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus / Complex 1 protein (LYR family) / NAD(P)H-quinone oxidoreductase subunit D/H / NADH-quinone oxidoreductase, chain 5-like / NADH:ubiquinone oxidoreductase, 49kDa subunit, conserved site / NADH-ubiquinone oxidoreductase chain 4L/Mnh complex subunit C1-like / NADH-ubiquinone/plastoquinone oxidoreductase chain 4L / Respiratory chain NADH dehydrogenase 49 Kd subunit signature. / NADH-quinone oxidoreductase, subunit D / NADH:ubiquinone oxidoreductase, subunit G / Respiratory-chain NADH dehydrogenase, 49 Kd subunit / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 3. / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 2. / NADH ubiquinone oxidoreductase, F subunit / NADH dehydrogenase, subunit C / NADH:ubiquinone oxidoreductase, 30kDa subunit, conserved site / Respiratory chain NADH dehydrogenase 30 Kd subunit signature.
Similarity search - Domain/homology
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial / NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 ...NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial / NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial / NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial / Complex I-15 kDa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 / NADH-ubiquinone oxidoreductase chain 1 / NADH-ubiquinone oxidoreductase chain 2 / NADH-ubiquinone oxidoreductase chain 3 / NADH-ubiquinone oxidoreductase chain 4L / NADH-ubiquinone oxidoreductase chain 4 / NADH-ubiquinone oxidoreductase chain 5 / PFC0360w protein / Acyl carrier protein / NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 / NADH dehydrogenase [ubiquinone] 1 subunit C2 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial / NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 / Flavoprotein/dehydrogenase
Similarity search - Component
Biological speciesOvis aries (sheep) / sheep (sheep)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.8 Å
AuthorsSazanov L / Petrova O
Funding supportEuropean Union, 1 items
OrganizationGrant numberCountry
European Research Council (ERC)101020697European Union
CitationJournal: Nature / Year: 2022
Title: A universal coupling mechanism of respiratory complex I.
Authors: Vladyslav Kravchuk / Olga Petrova / Domen Kampjut / Anna Wojciechowska-Bason / Zara Breese / Leonid Sazanov /
Abstract: Complex I is the first enzyme in the respiratory chain, which is responsible for energy production in mitochondria and bacteria. Complex I couples the transfer of two electrons from NADH to quinone ...Complex I is the first enzyme in the respiratory chain, which is responsible for energy production in mitochondria and bacteria. Complex I couples the transfer of two electrons from NADH to quinone and the translocation of four protons across the membrane, but the coupling mechanism remains contentious. Here we present cryo-electron microscopy structures of Escherichia coli complex I (EcCI) in different redox states, including catalytic turnover. EcCI exists mostly in the open state, in which the quinone cavity is exposed to the cytosol, allowing access for water molecules, which enable quinone movements. Unlike the mammalian paralogues, EcCI can convert to the closed state only during turnover, showing that closed and open states are genuine turnover intermediates. The open-to-closed transition results in the tightly engulfed quinone cavity being connected to the central axis of the membrane arm, a source of substrate protons. Consistently, the proportion of the closed state increases with increasing pH. We propose a detailed but straightforward and robust mechanism comprising a 'domino effect' series of proton transfers and electrostatic interactions: the forward wave ('dominoes stacking') primes the pump, and the reverse wave ('dominoes falling') results in the ejection of all pumped protons from the distal subunit NuoL. This mechanism explains why protons exit exclusively from the NuoL subunit and is supported by our mutagenesis data. We contend that this is a universal coupling mechanism of complex I and related enzymes.
History
DepositionMar 30, 2022-
Header (metadata) releaseSep 21, 2022-
Map releaseSep 21, 2022-
UpdateOct 5, 2022-
Current statusOct 5, 2022Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_14688.png

Downloads & links

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Map

FileDownload / File: emd_14688.map.gz / Format: CCP4 / Size: 19.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationcomposite map
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesX (Sec.)Y (Row.)Z (Col.)
1.22 Å/pix.
x 136 pix.
= 165.92 Å		1.22 Å/pix.
x 161 pix.
= 196.42 Å		1.22 Å/pix.
x 236 pix.
= 287.92 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

generated in cubic-lattice coordinate

Voxel sizeX=Y=Z: 1.22 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.077
Minimum - Maximum-0.07565412 - 0.5318821
Average (Standard dev.)0.010583327 (±0.029524699)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderZYX
Origin000
Dimensions161236136
Spacing136161236
CellA: 165.92 Å / B: 196.42 Å / C: 287.92 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Complex I from Ovis aries at pH9, Closed state

EntireName: Complex I from Ovis aries at pH9, Closed state
Components
  • Complex: Complex I from Ovis aries at pH9, Closed state
    • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 3
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 1
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 6
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 4L
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 5
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 4
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 2
    • Protein or peptide: Complex I-B14.7
    • Protein or peptide: Complex I-SGDH
    • Protein or peptide: Acyl carrier protein
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
    • Protein or peptide: Complex I-PDSW
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
    • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5
    • Protein or peptide: Complex I-B9
    • Protein or peptide: Complex I-B12
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 subunit C2
    • Protein or peptide: NADH:ubiquinone oxidoreductase subunit B4
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7
    • Protein or peptide: Complex I-B22
    • Protein or peptide: Complex I-AGGG
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
    • Protein or peptide: Complex I-ESSS
    • Protein or peptide: Complex I-KFYI
    • Protein or peptide: Complex I-MNLL
    • Protein or peptide: Complex I-MWFE
    • Protein or peptide: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
    • Protein or peptide: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
    • Protein or peptide: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
    • Protein or peptide: Complex I-30kD
    • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
    • Protein or peptide: Complex I-23kD
    • Protein or peptide: Complex I-9kD
    • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial
    • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
    • Protein or peptide: NADH:ubiquinone oxidoreductase subunit A9
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
    • Protein or peptide: Complex I-B14
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
  • Ligand: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE
  • Ligand: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate
  • Ligand: ADENOSINE MONOPHOSPHATE
  • Ligand: MYRISTIC ACID
  • Ligand: IRON/SULFUR CLUSTER
  • Ligand: FLAVIN MONONUCLEOTIDE
  • Ligand: 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE
  • Ligand: FE2/S2 (INORGANIC) CLUSTER
  • Ligand: POTASSIUM ION
  • Ligand: ZINC ION
  • Ligand: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

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Supramolecule #1: Complex I from Ovis aries at pH9, Closed state

SupramoleculeName: Complex I from Ovis aries at pH9, Closed state / type: complex / Chimera: Yes / ID: 1 / Parent: 0 / Macromolecule list: #1-#44
Source (natural)Organism: Ovis aries (sheep)
Molecular weightExperimental: 1 MDa

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Macromolecule #1: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

MacromoleculeName: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 49.22032 KDa
SequenceString: ARQWQPDVEW AEQYGGAVMY PTKETAHWKP PPWNDVDPPK DTLVSNLTLN FGPQHPAAHG VLRLVMELSG EMVRKCDPHI GLLH(2MR)GTEK LIEYKTYLQA LPYFDRLDYV SMMCNEQAYS LAVEKLLNIQ PPPRAQWIRV LFGEITRLLN HIMAVTT HA LDIGAMTPFF ...String:
ARQWQPDVEW AEQYGGAVMY PTKETAHWKP PPWNDVDPPK DTLVSNLTLN FGPQHPAAHG VLRLVMELSG EMVRKCDPHI GLLH(2MR)GTEK LIEYKTYLQA LPYFDRLDYV SMMCNEQAYS LAVEKLLNIQ PPPRAQWIRV LFGEITRLLN HIMAVTT HA LDIGAMTPFF WMFEEREKMF EFYERVSGAR MHAAYVRPGG VHQDLPLGLM DDIYEFSKNF SLRIDELEEM LTNNRIWR N RTVDIGVVTA EDALNYGFSG VMLRGSGIQW DLRKTQPYDV YDQVEFDVPI GSRGDCYDRY LCRVEEMRQS IRIISQCLN KMPPGEIKVD DAKVSPPKRA EMKTSMESLI HHFKLYTEGY QVPPGATYTA IEAPKGEFGV YLVSDGSSRP YRCKIKAPGF AHLAGLDKM SKGHMLADVV AIIGTQDIVF GEVDR

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Macromolecule #2: NADH-ubiquinone oxidoreductase chain 3

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 3 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 13.10652 KDa
SequenceString:
MNLMITLLTN FTLATLLVTI AFWLPQLNVY SEKTSPYECG FDPMGSARLP FSMKFFLVAI TFLLFDLEIA LLLPLPWASQ TTNLNTMLT MALLLIFLLA VSLAYEWTQK GLEWTE

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Macromolecule #3: NADH-ubiquinone oxidoreductase chain 1

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 1 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 35.884902 KDa
SequenceString: MFMINVLTLI IPILLAVAFL TLVERKVLGY MQFRKGPNVV GPYGLLQPIA DAIKLFIKEP LRPATSSISM FILAPILALT LALTMWIPL PMPYPLINMN LGVLFMLAMS SLAVYSILWS GWASNSKYAL IGALRAVAQT ISYEVTLAII LLSVLLMNGS F TLSTLIIT ...String:
MFMINVLTLI IPILLAVAFL TLVERKVLGY MQFRKGPNVV GPYGLLQPIA DAIKLFIKEP LRPATSSISM FILAPILALT LALTMWIPL PMPYPLINMN LGVLFMLAMS SLAVYSILWS GWASNSKYAL IGALRAVAQT ISYEVTLAII LLSVLLMNGS F TLSTLIIT QEQVWLIFPA WPLAMMWFIS TLAETNRAPF DLTEGESELV SGFNVEYAAG PFALFFMAEY ANIIMMNIFT TT LFLGAFH NPYMPELYTI NFTIKSLLLS ITFLWIRASY PRFRYDQLMH LLWKNFLPLT LALCMWHVSL PILLSSIPPQ T

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Macromolecule #4: NADH-ubiquinone oxidoreductase chain 6

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 6 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 19.126619 KDa
SequenceString:
MMTYIVFILS IIFVMGFVGF SSKPSPIYGG LGLIVSGGVG CGIVLNFGGS FLGLMVFLIY LGGMMVVFGY TTAMATEQYP EVWVSNKVV LGTFITGLLM EFLMVYYVLK DKEVEIVFKF NGMGDWVIYD TGDSGFFSEE AMGIAALYSY GTWLVIVTGW S LLIGVVVI MEITRGN

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Macromolecule #5: NADH-ubiquinone oxidoreductase chain 4L

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 4L / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 10.868237 KDa
SequenceString:
(FME)SLVYMNIMM AFTVSLTGLL MYRSHLMSSL LCLEGMMLSL FILATLMILN SHFTLASMMP IILLVFAACE AALGLS LLV MVSNTYGTDY VQNLNLLQC

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Macromolecule #6: NADH-ubiquinone oxidoreductase chain 5

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 5 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 68.438906 KDa
SequenceString: (FME)NLFSSLTLV TLILLTMPIA AINFNTHKFT NYPLYVKTTI SCAFITSMIP TMMFIHTGQE MIISNWHWLT IQTLKL SLS FKMDFFSMMF VPVALFVTWS IMEFSMWYMH SDPNINQFFK YLLLFLITML ILVTANNLFQ LFIGWEGVGI MSFLLIG WW YGRTDANTAA ...String:
(FME)NLFSSLTLV TLILLTMPIA AINFNTHKFT NYPLYVKTTI SCAFITSMIP TMMFIHTGQE MIISNWHWLT IQTLKL SLS FKMDFFSMMF VPVALFVTWS IMEFSMWYMH SDPNINQFFK YLLLFLITML ILVTANNLFQ LFIGWEGVGI MSFLLIG WW YGRTDANTAA LQAILYNRIG DIGFILAMAW FLINLNTWDL QQIFMLNPND SNLPLMGLIL AATGKSAQFG LHPWLPSA M EGPTPVSALL HSSTMVVAGI FLLIRFYPLT ENNKFGQSIM LCLGAMTTLF TAMCALTQND IKKIIAFSTS SQLGLMMVT IGINQPHLAF LHICTHAFFK AMLFMCSGSI IHSLNDEQDI RKMGGLFKAM PFTTTALIIG SLALTGMPFL TGFYSKDLII ESANTSYTN AWALLMTLVA TSFTAIYSTR IIFFALLGQP RFPTLININE NNPFLINSIK RLLIGSLFAG FIISNNIPPM T IPQMTMPH YLKMTALTVT ILGFILALEI SNTTHYLKFN YPSNTFKFSN LLGYYPTIMH RLTPYMNLTM SQKSASSLLD LI WLETILP KTISLAQMKM STTITSQKGL IKLYFLSFLI TILISTTLLN FHE

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Macromolecule #7: NADH-ubiquinone oxidoreductase chain 4

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 4 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 52.086742 KDa
SequenceString: (FME)LKYIIPTMM LMPLTWLSKN SMIWINTTLH SLLISLTSLL LLNQFGDNSL NFSLTFFSDS LSTPLLILTM WLLPLM LMA SQHHLSKENL ARKKLFISML ILLQLFLIMT FTATELIFFY IMFEATLVPT LIIITRWGNQ TERLNAGLYF LFYTLAG SL PLLVALIYIQ ...String:
(FME)LKYIIPTMM LMPLTWLSKN SMIWINTTLH SLLISLTSLL LLNQFGDNSL NFSLTFFSDS LSTPLLILTM WLLPLM LMA SQHHLSKENL ARKKLFISML ILLQLFLIMT FTATELIFFY IMFEATLVPT LIIITRWGNQ TERLNAGLYF LFYTLAG SL PLLVALIYIQ NTMGSLNFLI LQYWVQPMPN SWSNTFMWLA CMMAFMVKMP LYGLHLWLPK AHVEAPIAGS MVLAAILL K LGGYGMMRIT LLLNPITDFM AYPFIMLSLW GMIMTSSICL RQTDLKSLIA YSSVSHMALV IVAILIQTPW SYMGATALM IAHGLTSSML FCLANSNYER VHSRTMILAR GLQTLLPLMA AWWLLASLTN LALPPSINLI GELFVVMSTF SWSNITIILM GLNMVITAL YSLYMLITTQ RGKHTHHINN ILPSFTRENA LMSLHMLPLL LLSLNPKIIL GPLY

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Macromolecule #8: NADH-ubiquinone oxidoreductase chain 2

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 2 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 39.149805 KDa
SequenceString: MNPIILIIIL MTVMLGTIIV MISTHWLLIW IGFEMNMLAI IPIMMKKHNP RATEASTKYF LTQSTASMLL MMAIIINLMF SGQWTVMKL FNPMASMLMT MALAMKLGMA PFHFWVPEVT QGIPLSSGLI LLTWQKLAPM SVLYQILPSI NLDLILTLSI L SITIGGWG ...String:
MNPIILIIIL MTVMLGTIIV MISTHWLLIW IGFEMNMLAI IPIMMKKHNP RATEASTKYF LTQSTASMLL MMAIIINLMF SGQWTVMKL FNPMASMLMT MALAMKLGMA PFHFWVPEVT QGIPLSSGLI LLTWQKLAPM SVLYQILPSI NLDLILTLSI L SITIGGWG GLNQTQLRKI MAYSSIAHMG WMTAVLLYNP TMTLLNLIIY IIMTSTMFTL FMANSTTTTL SLSHTWNKAP IM TILVLIT LLSMGGLPPL SGFMPKWMII QEMTKNDSII LPTLMAITAL LNLYFYMRLT YSTALTMFPS TNNMKMKWQF PTT KRMTLL PTMTVLSTML LPLTPILSIL E

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Macromolecule #9: Complex I-B14.7

MacromoleculeName: Complex I-B14.7 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 14.652813 KDa
SequenceString:
(AYA)KTLLHQYWD IPEGTECHRK TYAATSIGGA SGLVVSAYSV ALKTPTSFLE GVARTGRYTF TAAAIGAIFG LTSCIS AQV REKPDDPLNY LIGGCAGGLT LGARTRSYGI GAAACAYMGL TAALVKMGQL EGWKVFAEPK V

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Macromolecule #10: Complex I-SGDH

MacromoleculeName: Complex I-SGDH / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 16.317004 KDa
SequenceString:
GKRLFIIKPS GFYDKRFLKL LRFYILLTGI PVVIGITLIN VFIGEAELAE IPEGYVPEHW EYFKHPISRW IARTFFDAPE KNYERTMAI LQIESEKAEL RLKELEVRRL MRAKGDGPWF QYPTIDKALI DHSPKATPDN

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Macromolecule #11: Acyl carrier protein

MacromoleculeName: Acyl carrier protein / type: protein_or_peptide / ID: 11 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 17.608199 KDa
SequenceString:
VEVKDFYTTN YQTAVSFSPL GPMPSMALMA VSLSGANVPK SGGRPEESRV PVLTQRKVPG RVTPLCRQYS DAPPLTLEGI KDRVLYVLK LYDKIDPEKL SVNSHFMKDL GLDSLDQVEI IMAMEDEFGF EIPDIDAEKL MCPQEIVDYI ADKKDVYE

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Macromolecule #12: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 20.008014 KDa
SequenceString:
PGIVELPSLE DLKVQEVKVS SSVLKAAAHH YGAQCDKPNK EFMLCRWEEK DPRRCLEEGK LVNQCALEFF RQIKRHCAEP FTEYWTCID YSGLQLFRRC RKEQAQFDKC VLDKLGWVRP DLGELSKVTK VKTDRPLPEN PYHSRARPEP NPEVEGDLKP A RHGSRLFF WTM

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Macromolecule #13: Complex I-PDSW

MacromoleculeName: Complex I-PDSW / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 20.526389 KDa
SequenceString:
SWDKDVYPEP PRRTPAPSPQ TSLPNPITYL TKAFDLLVDR PVTLVREFIE RQHAKNKYYY YHREFRRVPD ITECHEKDVL CMFEAEMQW KRDYKVDQEI VNIIQERLKA CQQREGESHR QNCAKELQQF TQVVKAYQDR YHDLGAHYSA RKCLAKQKQR M LAERKATK EAA

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Macromolecule #14: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mi...

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 36.883742 KDa
SequenceString: LQYGPLAYIL GEKTTKKMTE NSKLITVDGN ICSGK(SEP)KLAK EVAEKLGLKH FPEAGIHYAD STTGDGKPLP VRFSGN CSL EKFYDDPKSN DGNSYRLQAW LYASRLLQYA DALEHLLSTG QGVVLERSIY SDFVFLEAMY RQGFIRKQCV DHYNQVK KV TVCEYLPPHV ...String:
LQYGPLAYIL GEKTTKKMTE NSKLITVDGN ICSGK(SEP)KLAK EVAEKLGLKH FPEAGIHYAD STTGDGKPLP VRFSGN CSL EKFYDDPKSN DGNSYRLQAW LYASRLLQYA DALEHLLSTG QGVVLERSIY SDFVFLEAMY RQGFIRKQCV DHYNQVK KV TVCEYLPPHV VIYVDVPVSE VQSRIQKKGN PHEMKITSAY LQDIENVYKG TFLPEMSEKC EVLQYSAWEA EDAEKVVE D IQYLKYDKGP WLDQDDRKLH NLRMLVQDKL EVLNYTSIPV FLPEVTIGAH QSDRVFQEFT ELPGRKYRAG YNEDVGDKW IWLK

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Macromolecule #15: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5

MacromoleculeName: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 12.474397 KDa
SequenceString:
PFFDVQKKLG VDLDHWMTIQ SAEQPHRIPA RCHAFEKEWI ECAHGIGSIR AEKECKIEFE DFRECLLRQK TMKRLNAIKR QRDKLIKEG KYTPPPHHSG QEDLRP

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Macromolecule #16: Complex I-B9

MacromoleculeName: Complex I-B9 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 8.869269 KDa
SequenceString:
VAAFLKNVWA KEPVLVASFA IAGLAVILPT LSPYTKYSLM INRATPYNYP VPLRDDGNMP DVPSHPQDPQ GPSLEWLKRL

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Macromolecule #17: Complex I-B12

MacromoleculeName: Complex I-B12 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 8.958202 KDa
SequenceString:
SKMELPDYKQ WKIEGTPLET VQEKLAARGL RDPWGRNEAW RYMGGFANNV SFVGALLKGF KWGFAAFVVA VGAEYYLES

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Macromolecule #18: NADH dehydrogenase [ubiquinone] 1 subunit C2

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 subunit C2 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 14.23142 KDa
SequenceString:
MMTGRQARAP LQFLPDEARS LPPPKLTDPR LAYIGFLGYC SGLIDNAIRR RPVLSAGLHR QFLYITSFVF VGYYLLKRQD YMYAVRDHD MFSYIKSHPE DFPEKDKKTY REVFEEFHPV R

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Macromolecule #19: NADH:ubiquinone oxidoreductase subunit B4

MacromoleculeName: NADH:ubiquinone oxidoreductase subunit B4 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 14.975999 KDa
SequenceString:
SFPKYEPSRL ASLPTTLDPA EYDISSETRK AQAERLAIRS RLKREYQLQY NDPSRRGVVE DPALIRWTCA RSANVYPNFR PNTKTSLLG ALFGIGPLIF WYYVFKTDRD RKEKLIQEGK LDRTFNISY

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Macromolecule #20: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13
type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 16.276852 KDa
SequenceString:
VKQDMPPVGG YGPIDYKRNL PRRGLSGYSM FAVGIGALLF GYWSMMRWNR ERRRLQIEDF EARIALMPLL QAEKDRRVLQ MLRENLEEE ATIMKDVPGW KVGESVFHTT RWVTPMMGEL YGLRTGEEIL SSTYGFIWYT

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Macromolecule #21: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6
type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 15.296768 KDa
SequenceString:
SGYTPDEKLR LQQLRELRRR WLKDQELSPR EPVLPPRRVS PVERFWNKFL QDGALWKDVI YKTYRHSIFA FTHVLIPVWI IHYYLKYHV TTKPYTIVEK KPRIFPGDTI LETGEVIPPM KEFPDQH

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Macromolecule #22: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7
type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 14.963081 KDa
SequenceString:
GAHLARRYLG DASVEPEPLR MPTFPPDYGF PERKEREMVA TQQEMNDAQL VLQQRDYCAH YLIRFLKCKR DSFPNFLACK HEQHDWDYC EHLDYVKRMK EFERERRLLQ RKKRREQREA DMA

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Macromolecule #23: Complex I-B22

MacromoleculeName: Complex I-B22 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 21.517518 KDa
SequenceString:
AFLPSAAYLT HQQKVLQLYK RALRHLESRC VHRDKYRYFA CLLRARFDEH KNEKDMVKAT QLLREAEKEF WHGQHPQPYI FPESPGGTS YERYECYKVP EWCLDDWHPS EKAMYPDYFA KREQWKKLRR ESWEREVKQL QEETPVGGPR TEALPPARKQ G DLPPLWWH IVTRPRERP

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Macromolecule #24: Complex I-AGGG

MacromoleculeName: Complex I-AGGG / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 7.898769 KDa
SequenceString:
AHIEPRYRQF PQLTRSQVIQ AEFFSATMWF WILWRFWHDS DAVLGHFPYP DPSQWTDEEL GIPPD

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Macromolecule #25: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mito...

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 18.52177 KDa
SequenceString:
ITKDMLPGPY PKTPEERAAA AKKYNMRVED YEPYPDDGMG YGDYPKLPDR SQQERDPWYD WDHPDLRLNW GEPMHWDLDM YIRNRVDTS PTPVNWNLMC KHLFGFVAFM LFMFWVGETY PTYQPVGPKQ YPYNNLYLER GGDPNKEPEP VVHYEI

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Macromolecule #26: Complex I-ESSS

MacromoleculeName: Complex I-ESSS / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 11.998386 KDa
SequenceString:
TLRWQEDPEP EDENLYEKNP DSHGYDKDPA VDVWNMRVVF FFGFSIVLVL GSTFVAYLPD YRMQEWARRE AERLVKYREA HGLPLMESN CFDPSKIQLP ED

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Macromolecule #27: Complex I-KFYI

MacromoleculeName: Complex I-KFYI / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 5.808645 KDa
SequenceString:
KFYIQEPPHG SPNWLKVGLT LGTSAFLWIY LIKQHNEDVL EYKRRNGLE

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Macromolecule #28: Complex I-MNLL

MacromoleculeName: Complex I-MNLL / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 6.146106 KDa
SequenceString:
RDHWIHVLVP VGFVFGYYLD RKNDEKLAAF RNKSLLYKRE LKPQEEVTWK

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Macromolecule #29: Complex I-MWFE

MacromoleculeName: Complex I-MWFE / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 8.211519 KDa
SequenceString:
MWFEVLPGIA VMGVCLFIPG MATARIHRFS NGGREKRVAH YSYQWYLMER DRRVSGVNRY YVSKGLENID

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Macromolecule #30: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

MacromoleculeName: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 47.186676 KDa
SequenceString: KTSFGSLKDE DRIFTNLYGR HDWRLKGAQS RGDWYKTKEI LLKGPDWILG EVKTSGLRGR GGAGFPTGLK WSFMNKPSDG RPKYLVVNA DEGEPGTCKD REIIRHDPHK LVEGCLVGGR AMGARAAYIY IRGEFYNEAS NLQVAIREAY EAGLIGKNAC G SGYDFDVF ...String:
KTSFGSLKDE DRIFTNLYGR HDWRLKGAQS RGDWYKTKEI LLKGPDWILG EVKTSGLRGR GGAGFPTGLK WSFMNKPSDG RPKYLVVNA DEGEPGTCKD REIIRHDPHK LVEGCLVGGR AMGARAAYIY IRGEFYNEAS NLQVAIREAY EAGLIGKNAC G SGYDFDVF VVRGAGAYIC GEETALIESI EGKQGKPRLK PPFPADVGVF GCPTTVANVE TVAVSPTICR RGGAWFASFG RE RNSGTKL FNISGHVNNP CTVEEEMSVP LKELIEKHAG GVTGGWDNLL AVIPGGSSTP LIPKSVCETV LMDFDALIQA QTG LGTAAV IVMDRSTDIV KAIARLIEFY KHESCGQCTP CREGVDWMNK VMARFVRGDA RPAEIDSLWE ISKQIEGHTI CALG DGAAW PVQGLIRHFR PELEERMQRF AQQHQ

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Macromolecule #31: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

MacromoleculeName: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 23.598193 KDa
SequenceString: ALFVHRDTPK NNPETPFDFT PENYKRIEAI VKNYPEGHKA AAVLPVLDLA QRQNGWLPIS AMNKVAEILQ VPPMRVYEVA TFYTMYNRK PVGKYHIQVC TTTPCMLRNS DSILEAIQKK LGIKVGETTP DKLFTLIEVE CLGACVNAPM VQINDNYYED L TPKDIEEI ...String:
ALFVHRDTPK NNPETPFDFT PENYKRIEAI VKNYPEGHKA AAVLPVLDLA QRQNGWLPIS AMNKVAEILQ VPPMRVYEVA TFYTMYNRK PVGKYHIQVC TTTPCMLRNS DSILEAIQKK LGIKVGETTP DKLFTLIEVE CLGACVNAPM VQINDNYYED L TPKDIEEI IDELKAGKIP KPGPRSGRFS CEPAGGLTSL TEPPKGPGFG VQAGL

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Macromolecule #32: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

MacromoleculeName: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 75.520484 KDa
SequenceString: SNLIEVFVDG QSVMVEPGTT VLQACEKVGM QIPRFCYHER LSVAGNCRMC LVEIEKAPKV VAACAMPVMK GWNILTNSEK TKKAREGVM EFLLANHPLD CPICDQGGEC DLQDQSMMFG SDRSRFLEGK RAVEDKNIGP LVKTIMTRCI QCTRCIRFAS E IAGVDDLG ...String:
SNLIEVFVDG QSVMVEPGTT VLQACEKVGM QIPRFCYHER LSVAGNCRMC LVEIEKAPKV VAACAMPVMK GWNILTNSEK TKKAREGVM EFLLANHPLD CPICDQGGEC DLQDQSMMFG SDRSRFLEGK RAVEDKNIGP LVKTIMTRCI QCTRCIRFAS E IAGVDDLG TTGRGNDMQV GTYIEKMFMS ELSGNIIDIC PVGALTSKPY AFTARPWETR KTESIDVMDA VGSNIVVSTR TG EVMRILP RMHEDINEEW ISDKTRFAYD GLKRQRLTEP MVRNEKGLLT HTTWEDALSR VAGMLQSCQG NDVAAIAGGL VDA EALIAL KDLLNRVDSD TLCTEEVFPT AGAGTDLRSN YLLNTTIAGV EEADVVLLVG TNPRFEAPLF NARIRKSWLH NDLK VALIG SPVDLTYRYD HLGDSPKILQ DIASGSHPFS QVLQEAKKPM VVLGSSALQR NDGAAILAAV SNIAQKIRTS SGVTG DWKV MNILHRIASQ VAALDLGYKP GVEAIRKNPP KMLFLLGADG GCVTRQDLPK DCFIVYQGHH GDVGAPIADV ILPGAA YTE KSATYVNTEG RAQQTKVAVM PPGLAREDWK IIRALSEIAG MTLPYDTLDQ VRNRLEEVSP NLVRYDDVEG ANYFQQA SE LSKLVNQQLL ADPLVPPQLT IKDFYMTDSI SRASQTMAKC VKAVTE

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Macromolecule #33: Complex I-30kD

MacromoleculeName: Complex I-30kD / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 24.381525 KDa
SequenceString: TRPTVRPRND VAHKQLSAFG EYVAEILPKY VQQVQVSCFS ELEICIHPDG VIPVLTFLRD HSNAQFKSLA DLTAVDIPTR QNRFEIVYN LLSLRFNSRI RVKTYTDELT PVESSVSVYK AANWYEREIW DMFGVFFANH PDLRRILTDY GFEGHPFRKD F PLSGYVEL ...String:
TRPTVRPRND VAHKQLSAFG EYVAEILPKY VQQVQVSCFS ELEICIHPDG VIPVLTFLRD HSNAQFKSLA DLTAVDIPTR QNRFEIVYN LLSLRFNSRI RVKTYTDELT PVESSVSVYK AANWYEREIW DMFGVFFANH PDLRRILTDY GFEGHPFRKD F PLSGYVEL RYDDEVKRVV AEPVELAQEF RKFDLNSPWE AFPAYRQPPE

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Macromolecule #34: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial

MacromoleculeName: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 17.168219 KDa
SequenceString:
VAKLDDLINW ARRSSLWPMT FGLACCAVEM MHMAAPRYDM DRFGVVFRAS PRQSDVMIVA GTLTNKMAPA LRKVYDQMPE PRYVVSMGS CANGGGYYHY SYSVVRGCDR IVPVDIYVPG CPPTAEALLY GILQLQKKIK REKRLRIWYR R

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Macromolecule #35: Complex I-23kD

MacromoleculeName: Complex I-23kD / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 24.496121 KDa
SequenceString: MRKPKMRCLT MPVLLRALAQ AQAARAGHAS GRGLHSSAVA ATYKYVNLRE PSMDMKSVTD RAAQTLLWTE LIRGLGMTLS YLFREPATI NYPFEKGPLS PRFRGEHALR RYPSGEERCI ACKLCEAVCP AQAITIEAEP RADGSRRTTR YDIDMTKCIY C GFCQEACP ...String:
MRKPKMRCLT MPVLLRALAQ AQAARAGHAS GRGLHSSAVA ATYKYVNLRE PSMDMKSVTD RAAQTLLWTE LIRGLGMTLS YLFREPATI NYPFEKGPLS PRFRGEHALR RYPSGEERCI ACKLCEAVCP AQAITIEAEP RADGSRRTTR YDIDMTKCIY C GFCQEACP VDAIVEGPNF EFSTETHEEL LYNKEKLLNN GDKWEAEIAA NIQADYLYR

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Macromolecule #36: Complex I-9kD

MacromoleculeName: Complex I-9kD / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 5.26377 KDa
SequenceString:
PFDNTTYKNL QHHDYSTYTF LDLNLDLSKF RMPQPSSGRE SPRH

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Macromolecule #37: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial

MacromoleculeName: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial
type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 10.513649 KDa
SequenceString:
GVRTSPTGEK VTHTGQVYDD EDYRRVRFVG RQKEVNENFA IDLIAEQPVS QVGSRVISCD GGGGALGHPR VYINLDKETK TGTCGYCGL QFRQQH

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Macromolecule #38: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial

MacromoleculeName: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 14.559492 KDa
SequenceString:
TQLITVDEKL DITTLTGVPE EHIKTRKARI FVPARNNMQS GVNNTKKWKM EFDTRERWEN PLMGWASTAD PLSNLVLTFS TKEDAIAFA EKNGWSYDVE ERKVPKPKSK SYGANFSWNK RTRVSTK

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Macromolecule #39: NADH:ubiquinone oxidoreductase subunit A9

MacromoleculeName: NADH:ubiquinone oxidoreductase subunit A9 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 38.860719 KDa
SequenceString: LHHAVIPHGK GGRSSVSGIV ATVFGATGFL GRYLVNHLGR MGSQVIVPYR CEPYDTMHLR PMGDLGQIIF MDWNGRDKDS IRRAVEHSN VVINLVGREW ETKNFDFEDV FVKIPQAIAQ VSKEAGVEKF IHISHLNADI KSSSKYLRNK AVGEKEVRET F PEATIIKP ...String:
LHHAVIPHGK GGRSSVSGIV ATVFGATGFL GRYLVNHLGR MGSQVIVPYR CEPYDTMHLR PMGDLGQIIF MDWNGRDKDS IRRAVEHSN VVINLVGREW ETKNFDFEDV FVKIPQAIAQ VSKEAGVEKF IHISHLNADI KSSSKYLRNK AVGEKEVRET F PEATIIKP ADIFGREDRF LNYFANIRWF GGVPLISLGK KTVKQPVYIV DVTKGIINAI KDPDARGKTF AFVGPNRYLL FD LVQYVFA VAHRPFLPYP MPHFAYRWIG RLFEISPFEP WTTRDKVERI HTTDRTLPHL PGLEDLGVQA TPLELKAIEV LRR HRTYRW LSSEIEDVQP AKTV

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Macromolecule #40: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 9.841279 KDa
SequenceString:
LGLREIRIHL CQRSPGSQGV RDFIEKRYVE LKKANPDLPI LIRECSDVQP KLWARYAFGQ EKNVSLNNFS ADQVTRALEN VLSSKA

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Macromolecule #41: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 13.02818 KDa
SequenceString:
LLKKTTGLVG LAVCETPHER LKILYTKILD VLGHIPKNAA YRKYTEQITN EKLSIVKAEP DVKKLEEQLQ GGQIEEVILQ AENELSLAR KMIQWKPWEP LVEEPPASQW KWPI

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Macromolecule #42: Complex I-B14

MacromoleculeName: Complex I-B14 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 13.767958 KDa
SequenceString:
TSVKPIFSRD MNEAKRRVRE LYRAWYREVP NTVHLFQLDI SVKQGRDKVR EMFKKNAHVT DPRVVDLLVI KGKMELEETI NVWKQRTHV MRFFHETEAP RPKDFLSKFY VGHDP

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Macromolecule #43: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7
type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 12.441238 KDa
SequenceString:
(AYA)SATRLIQGL RNWASGRDLQ AKLQLRYQEI SKRTQPPPKL PVGPSHRLSN NYYCARDGRR EAMPPSIVMS SQKVLV AGK PAESSAVAAS EKKAVSPAPP IKRWELSQDE PYL

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Macromolecule #44: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: sheep (sheep)
Molecular weightTheoretical: 17.115508 KDa
SequenceString:
MELLQVLKRG LQQVSGHGGL RGYLRVLFRA NDVRVGTLVG EDKYGNKYYE DNKQFFGRHR WVIYTTEMNG KNTFWDVDGS MVPPEWHRW LHCMTDDPPT VKPPTARKFI WTNHKFNLSG TPQQYVPYST TRKKIQEWVP PSTPYK

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Macromolecule #45: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE

MacromoleculeName: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / type: ligand / ID: 45 / Number of copies: 5 / Formula: PC1
Molecular weightTheoretical: 790.145 Da
Chemical component information

ChemComp-PC1:
1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipid*YM

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Macromolecule #46: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-b...

MacromoleculeName: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate
type: ligand / ID: 46 / Number of copies: 2 / Formula: ZMP
Molecular weightTheoretical: 568.704 Da
Chemical component information

ChemComp-ZMP:
S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate

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Macromolecule #47: ADENOSINE MONOPHOSPHATE

MacromoleculeName: ADENOSINE MONOPHOSPHATE / type: ligand / ID: 47 / Number of copies: 1 / Formula: AMP
Molecular weightTheoretical: 347.221 Da
Chemical component information

ChemComp-AMP:
ADENOSINE MONOPHOSPHATE / AMP*YM

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Macromolecule #48: MYRISTIC ACID

MacromoleculeName: MYRISTIC ACID / type: ligand / ID: 48 / Number of copies: 1 / Formula: MYR
Molecular weightTheoretical: 228.371 Da
Chemical component information

ChemComp-MYR:
MYRISTIC ACID

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Macromolecule #49: IRON/SULFUR CLUSTER

MacromoleculeName: IRON/SULFUR CLUSTER / type: ligand / ID: 49 / Number of copies: 6 / Formula: SF4
Molecular weightTheoretical: 351.64 Da
Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER

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Macromolecule #50: FLAVIN MONONUCLEOTIDE

MacromoleculeName: FLAVIN MONONUCLEOTIDE / type: ligand / ID: 50 / Number of copies: 1 / Formula: FMN
Molecular weightTheoretical: 456.344 Da
Chemical component information

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

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Macromolecule #51: 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE

MacromoleculeName: 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE / type: ligand / ID: 51 / Number of copies: 1 / Formula: NAI
Molecular weightTheoretical: 665.441 Da
Chemical component information

ChemComp-NAI:
1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE

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Macromolecule #52: FE2/S2 (INORGANIC) CLUSTER

MacromoleculeName: FE2/S2 (INORGANIC) CLUSTER / type: ligand / ID: 52 / Number of copies: 2 / Formula: FES
Molecular weightTheoretical: 175.82 Da
Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

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Macromolecule #53: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 53 / Number of copies: 1 / Formula: K
Molecular weightTheoretical: 39.098 Da

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Macromolecule #54: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 54 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #55: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

MacromoleculeName: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
type: ligand / ID: 55 / Number of copies: 1 / Formula: NDP
Molecular weightTheoretical: 745.421 Da
Chemical component information

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration3 mg/mL
BufferpH: 9
GridModel: Quantifoil R0.6/1 / Material: COPPER
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeTFS GLACIOS
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Average exposure time: 3.3 sec. / Average electron dose: 90.0 e/Å2
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 120000
Sample stageCooling holder cryogen: NITROGEN

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Image processing

Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

6zkc

Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 41421
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelPDB ID:

6zkc

RefinementSpace: REAL / Protocol: FLEXIBLE FIT / Target criteria: correlation coefficient
Output model

PDB-7zeb:
Complex I from Ovis aries at pH9, Closed state

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