|Entry||Database: EMDB / ID: 1435|
|Title||Functional architecture of RNA polymerase I.|
|Sample||S. cerevisiae RNA polymerase I|
|Source||Saccharomyces cerevisiae / yeast / Budding yeast / サッカロミセス・セレビシエ /|
|Map data||This is the final reconstruction of RNA polymerase I in ccp4 format.|
|Method||single particle reconstruction, at 11.9 Å resolution|
|Authors||Kuhn C-D / Geiger SR / Baumli S / Gartmann M / Gerber J / Jennebach S / Mielke T / Tschochner H / Beckmann R / Cramer P|
|Citation||Cell, 2007, 131, 1260-1272|
|Date||Deposition: Sep 27, 2007 / Header (metadata) release: Sep 27, 2007 / Map release: Jan 8, 2008 / Last update: May 26, 2011|
Downloads & links
|File||emd_1435.map.gz (map file in CCP4 format, 6751 KB)|
|Projections & slices|
Images are generated by Spider package.
|Voxel size||X=Y=Z: 1.23 Å|
CCP4 map header:
-Entire S. cerevisiae RNA polymerase I
|Entire||Name: S. cerevisiae RNA polymerase I / Oligomeric State: monomeric / Number of components: 1|
|Mass||Theoretical: 600 kDa / Experimental: 600 kDa|
-Component #1: protein, RNA polymerase I
|Protein||Name: RNA polymerase I / a.k.a: DNA-dependant RNA polymerase I / Oligomeric Details: Monomer / Recombinant expression: Yes / Number of Copies: 1|
|Mass||Theoretical: 600 kDa / Experimental: 600 kDa|
|Source||Species: Saccharomyces cerevisiae / yeast / Budding yeast / サッカロミセス・セレビシエ / |
|Source (engineered)||Expression System: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ / |
|Source (natural)||Organelle: Nucleus / Location in cell: Nucleus / Cell: Yeast|
|External references||Gene Ontology: GO: 0005736|
|Sample solution||Specimen conc.: 0.1 mg/ml|
Buffer solution: 60mM ammonium sulfate, 5mM HEPES pH 7.8, 1mM magnesium chloride, 0.1mM zinc chloride
|Support film||Carbon holey grids|
|Vitrification||Instrument: FEI VITROBOT / Cryogen name: ETHANE / Humidity: 95 % / Details: Vitrification instrument: Vitrobot|
-Electron microscopy imaging
Model: Tecnai F30 / Image courtesy: FEI Company
|Imaging||Microscope: FEI TECNAI F30 / Date: Mar 20, 2006|
|Electron gun||Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 20 e/Å2 / Illumination mode: OTHER|
|Lens||Magnification: 39000 X (nominal) / Imaging mode: BRIGHT FIELD|
|Specimen Holder||Holder: Eucentric / Model: OTHER / Temperature: 100 K|
|Camera||Detector: KODAK SO-163 FILM|
|Image acquisition||Scanner: PRIMESCAN / Bit depth: 16 / Details: Heidelberg Drum Scanner|
|Processing||Method: single particle reconstruction / Number of projections: 46056 / Details: Quantifoil / Applied symmetry: C1 (asymmetric)|
|3D reconstruction||Algorithm: Projection matching / Software: SPIDER / Resolution: 11.9 Å / Resolution method: FSC 0.5|
-Atomic model buiding
|Modeling #1||Refinement protocol: rigid body|
Details: Protocol: rigid body. 1WCM (lacking Rpb4/7 and the foot domain) was fitted by manual docking using program O.
Input PDB model: 1WCM
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