ジャーナル: EMBO J / 年: 2007 タイトル: Cryo-electron microscopy of hepatitis B virions reveals variability in envelope capsid interactions. 著者: Stefan Seitz / Stephan Urban / Christoph Antoni / Bettina Böttcher / 要旨: Hepatitis B virus (HBV) is a major human pathogen causing about 750,000 deaths per year. The virion consists of a nucleocapsid and an envelope formed by lipids, and three integral membrane proteins. ...Hepatitis B virus (HBV) is a major human pathogen causing about 750,000 deaths per year. The virion consists of a nucleocapsid and an envelope formed by lipids, and three integral membrane proteins. Although we have detailed structural insights into the organization of the HBV core, the arrangement of the envelope in virions and its interaction with the nucleocapsid is elusive. Here we show the ultrastructure of hepatitis B virions purified from patient serum. We identified two morphological phenotypes, which appear as compact and gapped particles with nucleocapsids in distinguishable conformations. The overall structures of these nucleocapsids resemble recombinant cores with two alpha-helical spikes per asymmetric unit. At the charged tips the spikes are contacted by defined protrusions of the envelope proteins, probably via electrostatic interactions. The HBV envelope in the two morphotypes is to some extent variable, but the surface proteins follow a general packing scheme with up to three surface protein dimers per asymmetric unit. The variability in the structure of the envelope indicates that the nucleocapsid does not firmly constrain the arrangement of the surface proteins, but provides a general template for the packing.
名称: Hepatitis B virus / タイプ: sample / ID: 1000 / 詳細: virus purified from patients serum / Number unique components: 1
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超分子 #1: Hepatitis B virus
超分子
名称: Hepatitis B virus / タイプ: virus / ID: 1 / Name.synonym: HBV / NCBI-ID: 10407 / 生物種: Hepatitis B virus / ウイルスタイプ: VIRION / ウイルス・単離状態: SPECIES / ウイルス・エンベロープ: Yes / ウイルス・中空状態: No / Syn species name: HBV
宿主
生物種: Homo sapiens (ヒト) / 別称: VERTEBRATES
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実験情報
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構造解析
手法
クライオ電子顕微鏡法
解析
単粒子再構成法
試料の集合状態
particle
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試料調製
緩衝液
pH: 7.4
グリッド
詳細: 400 mesh copper rhodium grids (Maxtaform),
凍結
凍結剤: ETHANE / チャンバー内湿度: 90 % / チャンバー内温度: 93 K / 装置: HOMEMADE PLUNGER / 詳細: Vitrification instrument: self made 手法: automatic blot for 1-2 seconds from both sides before plunging