ジャーナル: EMBO J / 年: 2007 タイトル: Cryo-electron microscopy of hepatitis B virions reveals variability in envelope capsid interactions. 著者: Stefan Seitz / Stephan Urban / Christoph Antoni / Bettina Böttcher / 要旨: Hepatitis B virus (HBV) is a major human pathogen causing about 750,000 deaths per year. The virion consists of a nucleocapsid and an envelope formed by lipids, and three integral membrane proteins. ...Hepatitis B virus (HBV) is a major human pathogen causing about 750,000 deaths per year. The virion consists of a nucleocapsid and an envelope formed by lipids, and three integral membrane proteins. Although we have detailed structural insights into the organization of the HBV core, the arrangement of the envelope in virions and its interaction with the nucleocapsid is elusive. Here we show the ultrastructure of hepatitis B virions purified from patient serum. We identified two morphological phenotypes, which appear as compact and gapped particles with nucleocapsids in distinguishable conformations. The overall structures of these nucleocapsids resemble recombinant cores with two alpha-helical spikes per asymmetric unit. At the charged tips the spikes are contacted by defined protrusions of the envelope proteins, probably via electrostatic interactions. The HBV envelope in the two morphotypes is to some extent variable, but the surface proteins follow a general packing scheme with up to three surface protein dimers per asymmetric unit. The variability in the structure of the envelope indicates that the nucleocapsid does not firmly constrain the arrangement of the surface proteins, but provides a general template for the packing.
ダウンロード / ファイル: emd_1404.map.gz / 形式: CCP4 / 大きさ: 955.1 KB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
3d MAP OF SUB-SET OF HEPATITIS B VIRUS Particles (COMPACT PARTICLES; grouped in the same class)
ボクセルのサイズ
X=Y=Z: 8.4 Å
密度
表面レベル
1: 9.470000000000001 / ムービー #1: 2.6726856
最小 - 最大
-50.052799999999998 - 49.008200000000002
平均 (標準偏差)
0.0631176 (±6.61198)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
0
0
0
サイズ
63
63
63
Spacing
63
63
63
セル
A=B=C: 529.2 Å α=β=γ: 90 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
8.4
8.4
8.4
M x/y/z
63
63
63
origin x/y/z
0.000
0.000
0.000
length x/y/z
529.200
529.200
529.200
α/β/γ
90.000
90.000
90.000
start NX/NY/NZ
-50
-50
-50
NX/NY/NZ
100
100
100
MAP C/R/S
1
2
3
start NC/NR/NS
0
0
0
NC/NR/NS
63
63
63
D min/max/mean
-50.053
49.008
0.063
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添付データ
-
試料の構成要素
-
全体 : Hepatitis B virus
全体
名称: Hepatitis B virus (B 型肝炎ウイルス)
要素
試料: Hepatitis B virus
ウイルス: Hepatitis B virus (B 型肝炎ウイルス)
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超分子 #1000: Hepatitis B virus
超分子
名称: Hepatitis B virus / タイプ: sample / ID: 1000 / 詳細: virus purified from patients serum / Number unique components: 1
-
超分子 #1: Hepatitis B virus
超分子
名称: Hepatitis B virus / タイプ: virus / ID: 1 / Name.synonym: HBV / NCBI-ID: 10407 / 生物種: Hepatitis B virus / ウイルスタイプ: VIRION / ウイルス・単離状態: SPECIES / ウイルス・エンベロープ: Yes / ウイルス・中空状態: No / Syn species name: HBV
宿主
生物種: Homo sapiens (ヒト) / 別称: VERTEBRATES
-
実験情報
-
構造解析
手法
クライオ電子顕微鏡法
解析
単粒子再構成法
試料の集合状態
particle
-
試料調製
緩衝液
pH: 7.4
グリッド
詳細: 400 mesh copper rhodium grids (Maxtaform),
凍結
凍結剤: ETHANE / チャンバー内湿度: 90 % / チャンバー内温度: 93 K / 装置: HOMEMADE PLUNGER / 詳細: Vitrification instrument: self made 手法: automatic blot for 1-2 seconds from both sides before plunging
-
電子顕微鏡法
顕微鏡
FEI/PHILIPS CM120T
温度
平均: 178 K
アライメント法
Legacy - 非点収差: corrected at 200,000 times magnification