+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-13954 | ||||||||||||
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タイトル | Neuronal RNA granules are ribosome complexes stalled at the pre-translocation state | ||||||||||||
マップデータ | Postprocessed map (Relion) of rat ribosome from neuronal RNA granules. | ||||||||||||
試料 |
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キーワード | Ribosome / RNA granule / rat | ||||||||||||
機能・相同性 | 機能・相同性情報 regulation of myeloid dendritic cell activation / positive regulation of selenocysteine incorporation / Protein hydroxylation / mTORC1-mediated signalling / Translesion synthesis by REV1 / Recognition of DNA damage by PCNA-containing replication complex / APC/C:Cdc20 mediated degradation of Cyclin B / APC-Cdc20 mediated degradation of Nek2A / Activated NOTCH1 Transmits Signal to the Nucleus / Downregulation of TGF-beta receptor signaling ...regulation of myeloid dendritic cell activation / positive regulation of selenocysteine incorporation / Protein hydroxylation / mTORC1-mediated signalling / Translesion synthesis by REV1 / Recognition of DNA damage by PCNA-containing replication complex / APC/C:Cdc20 mediated degradation of Cyclin B / APC-Cdc20 mediated degradation of Nek2A / Activated NOTCH1 Transmits Signal to the Nucleus / Downregulation of TGF-beta receptor signaling / Regulation of FZD by ubiquitination / Regulation of TNFR1 signaling / TNFR1-induced NF-kappa-B signaling pathway / Translesion synthesis by POLK / Translesion synthesis by POLI / Termination of translesion DNA synthesis / Gap-filling DNA repair synthesis and ligation in GG-NER / Formation of TC-NER Pre-Incision Complex / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / Fanconi Anemia Pathway / Regulation of TP53 Degradation / Regulation of TP53 Activity through Methylation / Cyclin D associated events in G1 / Stabilization of p53 / PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1 / Synthesis of active ubiquitin: roles of E1 and E2 enzymes / ER Quality Control Compartment (ERQC) / Interferon alpha/beta signaling / Endosomal Sorting Complex Required For Transport (ESCRT) / Negative regulators of DDX58/IFIH1 signaling / Activation of IRF3, IRF7 mediated by TBK1, IKKε (IKBKE) / IKK complex recruitment mediated by RIP1 / IRAK2 mediated activation of TAK1 complex / TRAF6-mediated induction of TAK1 complex within TLR4 complex / Alpha-protein kinase 1 signaling pathway / Inactivation of CSF3 (G-CSF) signaling / IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation / Regulation of NF-kappa B signaling / Regulation of TBK1, IKKε (IKBKE)-mediated activation of IRF3, IRF7 / NOD1/2 Signaling Pathway / activated TAK1 mediates p38 MAPK activation / JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 / DNA Damage Recognition in GG-NER / Formation of Incision Complex in GG-NER / Dual Incision in GG-NER / E3 ubiquitin ligases ubiquitinate target proteins / Translesion Synthesis by POLH / Downregulation of ERBB2:ERBB3 signaling / TCF dependent signaling in response to WNT / Regulation of innate immune responses to cytosolic DNA / HDR through Homologous Recombination (HRR) / Downregulation of ERBB2 signaling / Regulation of signaling by CBL / Ovarian tumor domain proteases / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / NOTCH3 Activation and Transmission of Signal to the Nucleus / Interleukin-1 signaling / cellular response to Thyroid stimulating hormone / Downregulation of ERBB4 signaling / Deactivation of the beta-catenin transactivating complex / Negative regulation of MET activity / Translation initiation complex formation / Formation of the ternary complex, and subsequently, the 43S complex / Ribosomal scanning and start codon recognition / Protein methylation / Regulation of PTEN localization / L13a-mediated translational silencing of Ceruloplasmin expression / SRP-dependent cotranslational protein targeting to membrane / Josephin domain DUBs / Formation of a pool of free 40S subunits / GTP hydrolysis and joining of the 60S ribosomal subunit / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / RMTs methylate histone arginines / Major pathway of rRNA processing in the nucleolus and cytosol / Pexophagy / Stimuli-sensing channels / N-glycan trimming in the ER and Calnexin/Calreticulin cycle / 5.8S rRNA binding / Metalloprotease DUBs / Regulation of necroptotic cell death / Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha / Autodegradation of Cdh1 by Cdh1:APC/C / SCF-beta-TrCP mediated degradation of Emi1 / APC/C:Cdc20 mediated degradation of Securin / APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 / Cdc20:Phospho-APC/C mediated degradation of Cyclin A / SCF(Skp2)-mediated degradation of p27/p21 / NRIF signals cell death from the nucleus / NF-kB is activated and signals survival / Autodegradation of the E3 ubiquitin ligase COP1 / Asymmetric localization of PCP proteins / Degradation of DVL / Hedgehog ligand biogenesis / Dectin-1 mediated noncanonical NF-kB signaling / Degradation of GLI1 by the proteasome / Hedgehog 'on' state / TNFR2 non-canonical NF-kB pathway / NIK-->noncanonical NF-kB signaling 類似検索 - 分子機能 | ||||||||||||
生物種 | Rattus norvegicus (ドブネズミ) | ||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.86 Å | ||||||||||||
データ登録者 | Pulk A / Kipper K / Mansour A | ||||||||||||
資金援助 | Estonia, 3件
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引用 | ジャーナル: J Mol Biol / 年: 2022 タイトル: Neuronal RNA granules are ribosome complexes stalled at the pre-translocation state. 著者: Kalle Kipper / Abbas Mansour / Arto Pulk / 要旨: The polarized cell morphology of neurons dictates many neuronal processes, including the axodendridic transport of specific mRNAs and subsequent translation. mRNAs together with ribosomes and RNA- ...The polarized cell morphology of neurons dictates many neuronal processes, including the axodendridic transport of specific mRNAs and subsequent translation. mRNAs together with ribosomes and RNA-binding proteins form RNA granules that are targeted to axodendrites for localized translation in neurons. It has been established that localized protein synthesis in neurons is essential for long-term memory formation, synaptic plasticity, and neurodegeneration. We have used proteomics and electron microscopy to characterize neuronal RNA granules (nRNAg) isolated from rat brain tissues or human neuroblastoma. We show that ribosome-containing RNA granules are morula-like structures when visualized by electron microscopy. Crosslinking-coupled mass-spectrometry identified a potential G3BP2 binding site on the ribosome near the eIF3d-binding site on the 40S ribosomal subunit. We used cryo-EM to resolve the structure of the ribosome-component of nRNAg. The cryo-EM reveals that predominant particles in nRNAg are 80S ribosomes, resembling the pre-translocation state where tRNA's are in the hybrid A/P and P/E site. We also describe a new kind of principal motion of the ribosome, which we call the rocking motion. | ||||||||||||
履歴 |
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-構造の表示
添付画像 |
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-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_13954.map.gz | 17.5 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-13954-v30.xml emd-13954.xml | 106.6 KB 106.6 KB | 表示 表示 | EMDBヘッダ |
FSC (解像度算出) | emd_13954_fsc.xml | 10.5 KB | 表示 | FSCデータファイル |
画像 | emd_13954.png | 65 KB | ||
マスクデータ | emd_13954_msk_1.map | 101 MB | マスクマップ | |
Filedesc metadata | emd-13954.cif.gz | 21.5 KB | ||
その他 | emd_13954_additional_1.map.gz | 63.2 MB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-13954 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-13954 | HTTPS FTP |
-検証レポート
文書・要旨 | emd_13954_validation.pdf.gz | 553 KB | 表示 | EMDB検証レポート |
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文書・詳細版 | emd_13954_full_validation.pdf.gz | 552.6 KB | 表示 | |
XML形式データ | emd_13954_validation.xml.gz | 11.8 KB | 表示 | |
CIF形式データ | emd_13954_validation.cif.gz | 15.7 KB | 表示 | |
アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-13954 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-13954 | HTTPS FTP |
-関連構造データ
関連構造データ | 7qggMC M: このマップから作成された原子モデル C: 同じ文献を引用 (文献) |
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類似構造データ | 類似検索 - 機能・相同性F&H 検索 |
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_13954.map.gz / 形式: CCP4 / 大きさ: 101 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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注釈 | Postprocessed map (Relion) of rat ribosome from neuronal RNA granules. | ||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.41 Å | ||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-マスク #1
ファイル | emd_13954_msk_1.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-追加マップ: Sharpened map (Phenix) used to RealSpace refine the coordinates.
ファイル | emd_13954_additional_1.map | ||||||||||||
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注釈 | Sharpened map (Phenix) used to RealSpace refine the coordinates. | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-試料の構成要素
+全体 : Ribosome structure in the rat cortex-hippocampus derived neuronal...
+超分子 #1: Ribosome structure in the rat cortex-hippocampus derived neuronal...
+分子 #1: RNA (1872-MER)
+分子 #38: RNA (157-MER)
+分子 #39: RNA (121-MER)
+分子 #79: RNA (4803-MER)
+分子 #80: RNA (76-MER)
+分子 #81: RNA (76-MER)
+分子 #82: RNA (5'-D(P*()P*()P*()P*())-R(P*UP*UP*AP*CP*GP*GP*CP*GP*GP*UP*()P...
+分子 #2: 40S ribosomal protein SA
+分子 #3: 40S ribosomal protein S3a
+分子 #4: 40S ribosomal protein S3
+分子 #5: 40S ribosomal protein S4, X isoform
+分子 #6: 40S ribosomal protein S5
+分子 #7: 40S ribosomal protein S7
+分子 #8: 40S ribosomal protein S8
+分子 #9: 40S ribosomal protein S10
+分子 #10: 40S ribosomal protein S11
+分子 #11: 40S ribosomal protein S15
+分子 #12: Rps16 protein
+分子 #13: 40S ribosomal protein S17
+分子 #14: 40S ribosomal protein S18
+分子 #15: 40S ribosomal protein S19
+分子 #16: 40S ribosomal protein S20
+分子 #17: 40S ribosomal protein S21
+分子 #18: 40S ribosomal protein S23
+分子 #19: 40S ribosomal protein S26
+分子 #20: 40S ribosomal protein S28
+分子 #21: 40S ribosomal protein S29
+分子 #22: Receptor of activated protein C kinase 1
+分子 #23: 40S ribosomal protein S2
+分子 #24: 40S ribosomal protein S6
+分子 #25: 40S ribosomal protein S9
+分子 #26: 40S ribosomal protein S12
+分子 #27: 40S ribosomal protein S13
+分子 #28: 40S ribosomal protein S14
+分子 #29: 40S ribosomal protein S15a
+分子 #30: 40S ribosomal protein S24
+分子 #31: 40S ribosomal protein S25
+分子 #32: 40S ribosomal protein S27
+分子 #33: Ubiquitin-like domain-containing protein
+分子 #34: Ubiquitin-40S ribosomal protein S27a
+分子 #35: 60S ribosomal protein L8
+分子 #36: 60S ribosomal protein L3
+分子 #37: 60S ribosomal protein L4
+分子 #40: 60S ribosomal protein L5
+分子 #41: 60S ribosomal protein L6
+分子 #42: 60S ribosomal protein L7
+分子 #43: 60S ribosomal protein L7a
+分子 #44: 60S ribosomal protein L9
+分子 #45: 60S ribosomal protein L10
+分子 #46: 60S ribosomal protein L11
+分子 #47: 60S ribosomal protein L13
+分子 #48: 60S ribosomal protein L14
+分子 #49: Ribosomal protein L15
+分子 #50: 60S ribosomal protein L13a
+分子 #51: 60S ribosomal protein L17
+分子 #52: 60S ribosomal protein L18
+分子 #53: Ribosomal protein L19
+分子 #54: 60S ribosomal protein L18a
+分子 #55: 60S ribosomal protein L21
+分子 #56: 60S ribosomal protein L22
+分子 #57: 60S ribosomal protein L23
+分子 #58: 60S ribosomal protein L24
+分子 #59: 60S ribosomal protein L23a
+分子 #60: 60S ribosomal protein L26
+分子 #61: 60S ribosomal protein L27
+分子 #62: 60S ribosomal protein L27a
+分子 #63: 60S ribosomal protein L29
+分子 #64: 60S ribosomal protein L30
+分子 #65: 60S ribosomal protein L31
+分子 #66: 60S ribosomal protein L32
+分子 #67: 60S ribosomal protein L35a
+分子 #68: 60S ribosomal protein L34
+分子 #69: 60S ribosomal protein L35
+分子 #70: 60S ribosomal protein L36
+分子 #71: Ribosomal protein L37
+分子 #72: 60S ribosomal protein L38
+分子 #73: 60S ribosomal protein L39
+分子 #74: Ubiquitin-60S ribosomal protein L40
+分子 #75: 60S ribosomal protein L41
+分子 #76: Ribosomal protein L36a
+分子 #77: 60S ribosomal protein L37a
+分子 #78: 60S ribosomal protein L28
+分子 #83: 60S ribosomal protein L10a
+分子 #84: ALA-ALA-LYS-ALA
+分子 #85: MAGNESIUM ION
+分子 #86: ZINC ION
+分子 #87: water
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7.5 構成要素:
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グリッド | モデル: C-flat-1.2/1.3 / 材質: COPPER / メッシュ: 300 / 支持フィルム - 材質: CARBON / 支持フィルム - トポロジー: CONTINUOUS / 支持フィルム - Film thickness: 3 / 前処理 - タイプ: GLOW DISCHARGE / 前処理 - 時間: 10 sec. | |||||||||||||||
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 10 K / 装置: FEI VITROBOT MARK IV |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: FEI FALCON III (4k x 4k) 検出モード: INTEGRATING / 撮影したグリッド数: 1 / 実像数: 3644 / 平均電子線量: 30.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | C2レンズ絞り径: 70.0 µm / 照射モード: OTHER / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm / 最大 デフォーカス(公称値): 3.0 µm / 最小 デフォーカス(公称値): 0.5 µm / 倍率(公称値): 59000 |
試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER ホルダー冷却材: NITROGEN |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |