- EMDB-13898: The structure of Photosystem I tetramer from Chroococcidiopsis TS... -
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Basic information
Entry
Database: EMDB / ID: EMD-13898
Title
The structure of Photosystem I tetramer from Chroococcidiopsis TS-821, a thermophilic, unicellular, non-heterocyst-forming cyanobacterium
Map data
Sample
Complex: Photosystem I
Protein or peptide: x 11 types
Ligand: x 5 types
Function / homology
Function and homology information
thylakoid membrane / photosystem I reaction center / photosystem I / : / photosystem I / chlorophyll binding / photosynthetic electron transport in photosystem I / photosynthesis / 4 iron, 4 sulfur cluster binding / membrane => GO:0016020 ...thylakoid membrane / photosystem I reaction center / photosystem I / : / photosystem I / chlorophyll binding / photosynthetic electron transport in photosystem I / photosynthesis / 4 iron, 4 sulfur cluster binding / membrane => GO:0016020 / electron transfer activity / magnesium ion binding / metal ion binding Similarity search - Function
Photosystem I reaction center subunit PsaK / Photosystem I reaction centre subunit PsaK / Photosystem I PsaM, reaction centre superfamily / Photosystem I PsaM, reaction centre / Photosystem I protein M (PsaM) / Photosystem I reaction centre subunit PsaK superfamily / Photosystem I reaction center subunit V/PsaK / Photosystem I psaG / psaK / Photosystem I PsaL, reaction centre subunit XI / Photosystem I, reaction centre subunit XI ...Photosystem I reaction center subunit PsaK / Photosystem I reaction centre subunit PsaK / Photosystem I PsaM, reaction centre superfamily / Photosystem I PsaM, reaction centre / Photosystem I protein M (PsaM) / Photosystem I reaction centre subunit PsaK superfamily / Photosystem I reaction center subunit V/PsaK / Photosystem I psaG / psaK / Photosystem I PsaL, reaction centre subunit XI / Photosystem I, reaction centre subunit XI / Photosystem I PsaL, reaction centre subunit XI superfamily / Photosystem I reaction centre subunit XI / Photosystem I reaction centre subunit VIII / Photosystem I reaction centre subunit VIII / Photosystem I reaction centre subunit VIII superfamily / Photosystem I PsaF, reaction centre subunit III / Photosystem I PsaF, reaction centre subunit III superfamily / Photosystem I reaction centre subunit III / Photosystem I PsaJ, reaction centre subunit IX / Photosystem I PsaD / Photosystem I PsaJ, reaction centre subunit IX superfamily / Photosystem I, reaction centre subunit PsaD superfamily / Photosystem I reaction centre subunit IX / PsaJ / PsaD / Photosystem I PsaE, reaction centre subunit IV / Photosystem I reaction centre subunit IV / PsaE / Photosystem I protein PsaC / Photosystem I PsaA / Photosystem I PsaB / Photosystem I PsaA/PsaB, conserved site / Photosystem I psaA and psaB proteins signature. / Photosystem I PsaA/PsaB / Photosystem I PsaA/PsaB superfamily / Photosystem I psaA/psaB protein / Electron transport accessory-like domain superfamily / 4Fe-4S dicluster domain / 4Fe-4S ferredoxin, iron-sulphur binding, conserved site / 4Fe-4S ferredoxin-type iron-sulfur binding region signature. / 4Fe-4S ferredoxin-type iron-sulfur binding domain profile. / 4Fe-4S ferredoxin-type, iron-sulphur binding domain Similarity search - Domain/homology
Photosystem I reaction center subunit PsaK / Photosystem I reaction center subunit XII / Photosystem I iron-sulfur center / Photosystem I reaction center subunit VIII / PSI-F / Photosystem I P700 chlorophyll a apoprotein A1 / Photosystem I P700 chlorophyll a apoprotein A2 / Photosystem I reaction center subunit IV / Photosystem I reaction center subunit II / Photosystem I reaction center subunit IX / Photosystem I reaction center subunit XI Similarity search - Component
Biological species
Chroococcidiopsis sp. TS-821 (bacteria)
Method
single particle reconstruction / cryo EM / Resolution: 3.7 Å
Journal: Plant Commun / Year: 2022 Title: Cryo-EM structure of a tetrameric photosystem I from TS-821, a thermophilic, unicellular, non-heterocyst-forming cyanobacterium. Authors: Dmitry A Semchonok / Jyotirmoy Mondal / Connor J Cooper / Katrina Schlum / Meng Li / Muhamed Amin / Carlos O S Sorzano / Erney Ramírez-Aportela / Panagiotis L Kastritis / Egbert J Boekema / ...Authors: Dmitry A Semchonok / Jyotirmoy Mondal / Connor J Cooper / Katrina Schlum / Meng Li / Muhamed Amin / Carlos O S Sorzano / Erney Ramírez-Aportela / Panagiotis L Kastritis / Egbert J Boekema / Albert Guskov / Barry D Bruce / Abstract: Photosystem I (PSI) is one of two photosystems involved in oxygenic photosynthesis. PSI of cyanobacteria exists in monomeric, trimeric, and tetrameric forms, in contrast to the strictly monomeric ...Photosystem I (PSI) is one of two photosystems involved in oxygenic photosynthesis. PSI of cyanobacteria exists in monomeric, trimeric, and tetrameric forms, in contrast to the strictly monomeric form of PSI in plants and algae. The tetrameric organization raises questions about its structural, physiological, and evolutionary significance. Here we report the ∼3.72 Å resolution cryo-electron microscopy structure of tetrameric PSI from the thermophilic, unicellular cyanobacterium sp. TS-821. The structure resolves 44 subunits and 448 cofactor molecules. We conclude that the tetramer is arranged via two different interfaces resulting from a dimer-of-dimers organization. The localization of chlorophyll molecules permits an excitation energy pathway within and between adjacent monomers. Bioinformatics analysis reveals conserved regions in the PsaL subunit that correlate with the oligomeric state. Tetrameric PSI may function as a key evolutionary step between the trimeric and monomeric forms of PSI organization in photosynthetic organisms.
Macromolecule #7: Photosystem I reaction center subunit VIII
Macromolecule
Name: Photosystem I reaction center subunit VIII / type: protein_or_peptide / ID: 7 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)
Organism: Chroococcidiopsis sp. TS-821 (bacteria)
Molecular weight
Theoretical: 4.194028 KDa
Sequence
String:
MFSASFLPSI LVPLTGLVFP AIAMALLLIY VEREDPSGI
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Macromolecule #8: Photosystem I reaction center subunit IX
Macromolecule
Name: Photosystem I reaction center subunit IX / type: protein_or_peptide / ID: 8 / Details: BCR is not the part of the protein sequence / Number of copies: 4 / Enantiomer: LEVO
Macromolecule #9: Photosystem I reaction center subunit PsaK
Macromolecule
Name: Photosystem I reaction center subunit PsaK / type: protein_or_peptide / ID: 9 / Details: CLA is not the part of the protein sequence / Number of copies: 4 / Enantiomer: LEVO
Model: Quantifoil R2/2 / Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Support film - topology: HOLEY / Support film - Film thickness: 2.0 nm / Pretreatment - Type: GLOW DISCHARGE
Vitrification
Cryogen name: ETHANE / Chamber humidity: 90 % / Chamber temperature: 293 K / Instrument: FEI VITROBOT MARK III
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Electron microscopy
Microscope
FEI TITAN KRIOS
Image recording
Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Digitization - Dimensions - Width: 3838 pixel / Digitization - Dimensions - Height: 3710 pixel / Number real images: 4845 / Average exposure time: 12.0 sec. / Average electron dose: 4.3 e/Å2
Electron beam
Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
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