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- EMDB-13898: The structure of Photosystem I tetramer from Chroococcidiopsis TS... -

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Basic information

Entry
Database: EMDB / ID: EMD-13898
TitleThe structure of Photosystem I tetramer from Chroococcidiopsis TS-821, a thermophilic, unicellular, non-heterocyst-forming cyanobacterium
Map data
Sample
  • Complex: Photosystem I
    • Protein or peptide: x 11 types
  • Ligand: x 5 types
Function / homology
Function and homology information


thylakoid membrane / photosystem I reaction center / photosystem I / photosynthetic electron transport in photosystem I / : / photosystem I / chlorophyll binding / photosynthesis / 4 iron, 4 sulfur cluster binding / electron transfer activity ...thylakoid membrane / photosystem I reaction center / photosystem I / photosynthetic electron transport in photosystem I / : / photosystem I / chlorophyll binding / photosynthesis / 4 iron, 4 sulfur cluster binding / electron transfer activity / membrane => GO:0016020 / magnesium ion binding / metal ion binding
Similarity search - Function
Photosystem I reaction center subunit PsaK / Photosystem I reaction centre subunit PsaK / Photosystem I PsaM, reaction centre superfamily / Photosystem I PsaM, reaction centre / Photosystem I protein M (PsaM) / Photosystem I reaction centre subunit PsaK superfamily / Photosystem I reaction center subunit V/PsaK / Photosystem I psaG / psaK / Photosystem I PsaL, reaction centre subunit XI / Photosystem I, reaction centre subunit XI ...Photosystem I reaction center subunit PsaK / Photosystem I reaction centre subunit PsaK / Photosystem I PsaM, reaction centre superfamily / Photosystem I PsaM, reaction centre / Photosystem I protein M (PsaM) / Photosystem I reaction centre subunit PsaK superfamily / Photosystem I reaction center subunit V/PsaK / Photosystem I psaG / psaK / Photosystem I PsaL, reaction centre subunit XI / Photosystem I, reaction centre subunit XI / Photosystem I PsaL, reaction centre subunit XI superfamily / Photosystem I reaction centre subunit XI / Photosystem I reaction centre subunit VIII / Photosystem I reaction centre subunit VIII / Photosystem I reaction centre subunit VIII superfamily / Photosystem I PsaF, reaction centre subunit III / Photosystem I PsaF, reaction centre subunit III superfamily / Photosystem I reaction centre subunit III / Photosystem I PsaJ, reaction centre subunit IX / Photosystem I PsaD / Photosystem I PsaJ, reaction centre subunit IX superfamily / Photosystem I, reaction centre subunit PsaD superfamily / Photosystem I reaction centre subunit IX / PsaJ / PsaD / Photosystem I PsaE, reaction centre subunit IV / Photosystem I reaction centre subunit IV / PsaE / Photosystem I protein PsaC / Photosystem I PsaA / Photosystem I PsaB / Photosystem I PsaA/PsaB, conserved site / Photosystem I psaA and psaB proteins signature. / Photosystem I PsaA/PsaB / Photosystem I PsaA/PsaB superfamily / Photosystem I psaA/psaB protein / Electron transport accessory-like domain superfamily / 4Fe-4S dicluster domain / 4Fe-4S ferredoxin, iron-sulphur binding, conserved site / 4Fe-4S ferredoxin-type iron-sulfur binding region signature. / 4Fe-4S ferredoxin-type iron-sulfur binding domain profile. / 4Fe-4S ferredoxin-type, iron-sulphur binding domain
Similarity search - Domain/homology
Photosystem I reaction center subunit PsaK / Photosystem I reaction center subunit XII / Photosystem I iron-sulfur center / Photosystem I reaction center subunit VIII / PSI-F / Photosystem I P700 chlorophyll a apoprotein A1 / Photosystem I P700 chlorophyll a apoprotein A2 / Photosystem I reaction center subunit IV / Photosystem I reaction center subunit II / Photosystem I reaction center subunit IX / Photosystem I reaction center subunit XI
Similarity search - Component
Biological speciesChroococcidiopsis sp. TS-821 (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.7 Å
AuthorsSemchonok DA / Mondal J / Cooper JC / Schlum K / Li M / Amin M / Sorzano COS / Ramirez-Aportela E / Kastritis PL / Boekema EJ ...Semchonok DA / Mondal J / Cooper JC / Schlum K / Li M / Amin M / Sorzano COS / Ramirez-Aportela E / Kastritis PL / Boekema EJ / Guskov A / Bruce BD
Funding support United States, 3 items
OrganizationGrant numberCountry
National Science Foundation (NSF, United States)DGE-0801470 United States
National Science Foundation (NSF, United States)EPS-1004083 United States
National Science Foundation (NSF, United States)2017219379 United States
CitationJournal: Plant Commun / Year: 2022
Title: Cryo-EM structure of a tetrameric photosystem I from TS-821, a thermophilic, unicellular, non-heterocyst-forming cyanobacterium.
Authors: Dmitry A Semchonok / Jyotirmoy Mondal / Connor J Cooper / Katrina Schlum / Meng Li / Muhamed Amin / Carlos O S Sorzano / Erney Ramírez-Aportela / Panagiotis L Kastritis / Egbert J Boekema / ...Authors: Dmitry A Semchonok / Jyotirmoy Mondal / Connor J Cooper / Katrina Schlum / Meng Li / Muhamed Amin / Carlos O S Sorzano / Erney Ramírez-Aportela / Panagiotis L Kastritis / Egbert J Boekema / Albert Guskov / Barry D Bruce /
Abstract: Photosystem I (PSI) is one of two photosystems involved in oxygenic photosynthesis. PSI of cyanobacteria exists in monomeric, trimeric, and tetrameric forms, in contrast to the strictly monomeric ...Photosystem I (PSI) is one of two photosystems involved in oxygenic photosynthesis. PSI of cyanobacteria exists in monomeric, trimeric, and tetrameric forms, in contrast to the strictly monomeric form of PSI in plants and algae. The tetrameric organization raises questions about its structural, physiological, and evolutionary significance. Here we report the ∼3.72 Å resolution cryo-electron microscopy structure of tetrameric PSI from the thermophilic, unicellular cyanobacterium sp. TS-821. The structure resolves 44 subunits and 448 cofactor molecules. We conclude that the tetramer is arranged via two different interfaces resulting from a dimer-of-dimers organization. The localization of chlorophyll molecules permits an excitation energy pathway within and between adjacent monomers. Bioinformatics analysis reveals conserved regions in the PsaL subunit that correlate with the oligomeric state. Tetrameric PSI may function as a key evolutionary step between the trimeric and monomeric forms of PSI organization in photosynthetic organisms.
History
DepositionNov 24, 2021-
SupersessionApr 6, 2022ID: EMD-4659
Header (metadata) releaseApr 6, 2022-
Map releaseApr 6, 2022-
UpdateApr 6, 2022-
Current statusApr 6, 2022Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_13898.png

Downloads & links

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Map

FileDownload / File: emd_13898.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.11 Å/pix.
x 384 pix.
= 425.472 Å		1.11 Å/pix.
x 384 pix.
= 425.472 Å		1.11 Å/pix.
x 384 pix.
= 425.472 Å

Surface

Projections

Slices (1/3)

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Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.108 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0125
Minimum - Maximum-0.028560929 - 0.053150836
Average (Standard dev.)0.00027401146 (±0.0028294416)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions384384384
Spacing384384384
CellA=B=C: 425.47202 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_13898_msk_1.map
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_13898_half_map_1.map
Projections & Slices
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Histogram

Histogram (log scale)

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Half map: #1

Fileemd_13898_half_map_2.map
Projections & Slices
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Histogram (log scale)

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Sample components

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Entire : Photosystem I

EntireName: Photosystem I
Components
  • Complex: Photosystem I
    • Protein or peptide: Photosystem I P700 chlorophyll a apoprotein A1
    • Protein or peptide: Photosystem I P700 chlorophyll a apoprotein A2
    • Protein or peptide: Photosystem I iron-sulfur center
    • Protein or peptide: Photosystem I reaction center subunit II
    • Protein or peptide: Photosystem I reaction center subunit IV
    • Protein or peptide: Photosystem I reaction center subunit III
    • Protein or peptide: Photosystem I reaction center subunit VIII
    • Protein or peptide: Photosystem I reaction center subunit IX
    • Protein or peptide: Photosystem I reaction center subunit PsaK
    • Protein or peptide: Photosystem I reaction center subunit XI
    • Protein or peptide: Photosystem I reaction center subunit XII
  • Ligand: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
  • Ligand: CHLOROPHYLL A
  • Ligand: PHYLLOQUINONEPhytomenadione
  • Ligand: BETA-CAROTENEΒ-Carotene
  • Ligand: IRON/SULFUR CLUSTERIron–sulfur cluster

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Supramolecule #1: Photosystem I

SupramoleculeName: Photosystem I / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#11
Source (natural)Organism: Chroococcidiopsis sp. TS-821 (bacteria)
Molecular weightExperimental: 1.6 MDa

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Macromolecule #1: Photosystem I P700 chlorophyll a apoprotein A1

MacromoleculeName: Photosystem I P700 chlorophyll a apoprotein A1 / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO / EC number: photosystem I
Source (natural)Organism: Chroococcidiopsis sp. TS-821 (bacteria)
Molecular weightTheoretical: 83.352641 KDa
SequenceString: MTMSPPEREE KKARVIVDND PVPTSFEKWG QPGHFDRTLA KGPKTTTWIW NLHALAHDFD THTSDLEDIS RKIFAAHFGQ LAVIFLWLS GMEFHGARFS NYEAWLSDPI GIKPSAQVVW PIVGQDILNA DVGGGFHGIQ ITSGLFQVWR AAGFTNSFQL Y VTAIGGLV ...String:
MTMSPPEREE KKARVIVDND PVPTSFEKWG QPGHFDRTLA KGPKTTTWIW NLHALAHDFD THTSDLEDIS RKIFAAHFGQ LAVIFLWLS GMEFHGARFS NYEAWLSDPI GIKPSAQVVW PIVGQDILNA DVGGGFHGIQ ITSGLFQVWR AAGFTNSFQL Y VTAIGGLV AAALMLFAGW FHYHKRAPKL EWFQNAESML NHHLAGLLGL GCLSWAGHQI HVALPVNKLL DAGVAPKDIP LP HEFILNN SLMAELYPSF AKGLTPFWTL NWGQYADFLT FKGGLNPVTG GLWLTDTAHH HLALAVLFIV AGHMYRTNWG IGH SIKEIL ENHKGPFTGD GHKGLYENMT TSWHAQLGTN LAMLGSLTII VAHHMYAMPP YPYLATDYAT ALSIFTHHMW IGGF LIVGG AAHATIFMVR DYDPAVNQNN VLDRVIRHRD AIISHLNWVC MFLGFHSFGL YIHNDTMQAL GRPQDMFSDT AIQLQ PVFA QFVQNIHTLA PGSTAPNALE PVSYAFGGGV VAVGGKIAMM PIALGTADFM IHHIHAFQIH VTVLILLKGF LFARNS RLI PDKANLGFRF PCDGPGRGGT CQVSGWDHVF LGLFWMFNTI AIAVYHFSWK MQSDVWGTID PDGTIAHITG GNFAMSA NT INGWLRDYQW AQAAQVIQSY GSALSAYGLL FLGAHFVWAF SLMFLFSGRG YWQELIESIV WAHNKLKVAP TIQPRALS I IQGRAVGVAH YLLGAIVTIW AFFEARIISV G

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Macromolecule #2: Photosystem I P700 chlorophyll a apoprotein A2

MacromoleculeName: Photosystem I P700 chlorophyll a apoprotein A2 / type: protein_or_peptide / ID: 2 / Number of copies: 4 / Enantiomer: LEVO / EC number: photosystem I
Source (natural)Organism: Chroococcidiopsis sp. TS-821 (bacteria)
Molecular weightTheoretical: 82.656156 KDa
SequenceString: MATKFPKFSQ DLAQDPTTRR LWYGIATAHD FETHDGMTEE NLYQKLFATH FGHVAIIFLW TSSLLFHVAW QGNFEQWIKD PLNVRPIAH AIWDPHFGKA AVDAFTQAGA TYPVNIAYSG VYHWWYTIGM RTNNDLYSGA VFLLILAGVF LFAGWLHLQP K FRPSLSWF ...String:
MATKFPKFSQ DLAQDPTTRR LWYGIATAHD FETHDGMTEE NLYQKLFATH FGHVAIIFLW TSSLLFHVAW QGNFEQWIKD PLNVRPIAH AIWDPHFGKA AVDAFTQAGA TYPVNIAYSG VYHWWYTIGM RTNNDLYSGA VFLLILAGVF LFAGWLHLQP K FRPSLSWF KSAEPRLNHH LAGLFGVSSL AWTGHLVHVA IPESRGQHVG WDNFLTTLPH PEGLRPFFTG NWGVYAANPD TA NHVFGTS QGAGTAILTF LGGFHPQTQS LWLTDMAHHH LAIAVLFIIA GHMYRTNFGI GHSIKEMLNA KNFFGTKTEG QFN LPHQGL YDTINNSLHF QLSLALAALG TITSLVAQHM YALPPYAFMG QDFTTQAALY THHQYIAVFL MVGAFAHAGI FWVR DYDAE QNKGNVLDRV LQHKEAIISH LSWVSLFLGF HTLGLYVHND VVVAFGTPEK QILIEPVFAQ FIQASHGKVL YGLNT LLSN PDSVASTAGA AWLPGWLDAI NNGTNSLFLT IGPGDFLVHH AFALGIHTTV LVLVKGALDA RGSKLMPDKK DFGYAF PCD GPGRGGTCDI SAWDAFYLAL FWALNTVGWV TFYWHWKHLG IWQGNVAQFN ESSTYLMGWL RDYLWLYSAQ LINGYNP YG MNNLSVWAWM FLFGHLVWAT GFMFLISWRG YWQELIETLV WAHERTPLAS LVRWKDKPVA MSIVQGRLVG LAHFTVGY V LTYAAFVIAS TAGKFG

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Macromolecule #3: Photosystem I iron-sulfur center

MacromoleculeName: Photosystem I iron-sulfur center / type: protein_or_peptide / ID: 3 / Number of copies: 4 / Enantiomer: LEVO / EC number: photosystem I
Source (natural)Organism: Chroococcidiopsis sp. TS-821 (bacteria)
Molecular weightTheoretical: 8.939309 KDa
SequenceString:
MSHSVKIYDT CIGCTQCVRA CPTDVLEMVP WDGCKAGQIA ASPRTEDCVG CKRCETACPT DFLSIRVYLS NAETTRSMGL AY

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Macromolecule #4: Photosystem I reaction center subunit II

MacromoleculeName: Photosystem I reaction center subunit II / type: protein_or_peptide / ID: 4 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Chroococcidiopsis sp. TS-821 (bacteria)
Molecular weightTheoretical: 18.520025 KDa
SequenceString:
MAEELSGQTP IFGGSTGGLL TKAFREEKYV ITWTSPKEQV FEMPTGGAAI MRQGPNKLEL ARKEYCIALG GQQLRAKFRI TDYKIYRVY PNGEVQYLHP SDGVFPEKVN EGRQKVGYVD RNIGKNPDPA KLKFSGMTTY NAPGPKSSEA GKGSQDTKSG S LPRQGIEM

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Macromolecule #5: Photosystem I reaction center subunit IV

MacromoleculeName: Photosystem I reaction center subunit IV / type: protein_or_peptide / ID: 5 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Chroococcidiopsis sp. TS-821 (bacteria)
Molecular weightTheoretical: 13.551099 KDa
SequenceString:
MVERGSKVRI LRKESYWFQD VGVVASVDKS GIRYPVIVRF DKVNYAGVNT NNFAETELVE IEKPTAKAKK ATPAATGGKQ TTIDERTRT TGQGTPLKPE AKTTAAPESG EGSSTVVGDA NQGTESR

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Macromolecule #6: Photosystem I reaction center subunit III

MacromoleculeName: Photosystem I reaction center subunit III / type: protein_or_peptide / ID: 6 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Chroococcidiopsis sp. TS-821 (bacteria)
Molecular weightTheoretical: 18.131969 KDa
SequenceString:
MRRLFALVLV ISLWFSFAPQ ALAVGAGLVP CSESPEFIQR AASARNTTAD PNSGQKRFER YAQELCGPEG LPHLIVDGRL NHAGDFLIP SILFLYIAGW IGWVGRAYLQ EIKKRDNTEL REVIIDVPTA LPLMLSGFTW PLAAVKELLS GELVAKDDEI P VSPR

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Macromolecule #7: Photosystem I reaction center subunit VIII

MacromoleculeName: Photosystem I reaction center subunit VIII / type: protein_or_peptide / ID: 7 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Chroococcidiopsis sp. TS-821 (bacteria)
Molecular weightTheoretical: 4.194028 KDa
SequenceString:
MFSASFLPSI LVPLTGLVFP AIAMALLLIY VEREDPSGI

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Macromolecule #8: Photosystem I reaction center subunit IX

MacromoleculeName: Photosystem I reaction center subunit IX / type: protein_or_peptide / ID: 8 / Details: BCR is not the part of the protein sequence / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Chroococcidiopsis sp. TS-821 (bacteria)
Molecular weightTheoretical: 5.760832 KDa
SequenceString:
MQKKGDNQNY LLRYLSLGPV LLFAWLSFTA VLLIVFNYLY PDLLFHPLP

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Macromolecule #9: Photosystem I reaction center subunit PsaK

MacromoleculeName: Photosystem I reaction center subunit PsaK / type: protein_or_peptide / ID: 9 / Details: CLA is not the part of the protein sequence / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Chroococcidiopsis sp. TS-821 (bacteria)
Molecular weightTheoretical: 9.75361 KDa
SequenceString:
MTDLILLAAV QSTVPDTVRW SWTGSAIIIG ASLVILFIAS RTIRFPQVGP KMPLPFPALF NNPSVGTVLG SVSLGHIVGI GIILGLNNL GILK

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Macromolecule #10: Photosystem I reaction center subunit XI

MacromoleculeName: Photosystem I reaction center subunit XI / type: protein_or_peptide / ID: 10 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Chroococcidiopsis sp. TS-821 (bacteria)
Molecular weightTheoretical: 18.503156 KDa
SequenceString:
MAQAIDASKN RPSDPRNQEV VYPSRRDPQI GNLETPINNS SLVKWFINNL PAYRPGITPL RRGLEVGMAH GYWLLGPFAK LGPLRDTDV ANIAGLLATL GMVVISTLAV SLYAATDPPK PVATVTVPNP PDTFDTTEGW NTFASGFLIG GVGGAVVAYF I LANIELIQ NIFK

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Macromolecule #11: Photosystem I reaction center subunit XII

MacromoleculeName: Photosystem I reaction center subunit XII / type: protein_or_peptide / ID: 11 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Chroococcidiopsis sp. TS-821 (bacteria)
Molecular weightTheoretical: 3.382063 KDa
SequenceString:
MSISDTQVFV ALVIALIPGV LAFRLATELY K

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Macromolecule #12: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE

MacromoleculeName: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / type: ligand / ID: 12 / Number of copies: 5 / Formula: LHG
Molecular weightTheoretical: 722.97 Da
Chemical component information

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM / Phosphatidylglycerol

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Macromolecule #13: CHLOROPHYLL A

MacromoleculeName: CHLOROPHYLL A / type: ligand / ID: 13 / Number of copies: 359 / Formula: CLA
Molecular weightTheoretical: 893.489 Da
Chemical component information

ChemComp-CLA:
CHLOROPHYLL A / Chlorophyll a

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Macromolecule #14: PHYLLOQUINONE

MacromoleculeName: PHYLLOQUINONE / type: ligand / ID: 14 / Number of copies: 8 / Formula: PQN
Molecular weightTheoretical: 450.696 Da
Chemical component information

ChemComp-PQN:
PHYLLOQUINONE / Phytomenadione

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Macromolecule #15: BETA-CAROTENE

MacromoleculeName: BETA-CAROTENE / type: ligand / ID: 15 / Number of copies: 78 / Formula: BCR
Molecular weightTheoretical: 536.873 Da
Chemical component information

ChemComp-BCR:
BETA-CAROTENE / Β-Carotene

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Macromolecule #16: IRON/SULFUR CLUSTER

MacromoleculeName: IRON/SULFUR CLUSTER / type: ligand / ID: 16 / Number of copies: 12 / Formula: SF4
Molecular weightTheoretical: 351.64 Da
Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.32 mg/mL
BufferpH: 6.5
Component:
ConcentrationFormulaName
50.0 mMC6H13NO4SMES
10.0 mMMgCl2magnesium chloride
5.0 mMCaCl2calcium chloride
0.01 %C24H46O11DDM
GridModel: Quantifoil R2/2 / Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Support film - topology: HOLEY / Support film - Film thickness: 2.0 nm / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE / Chamber humidity: 90 % / Chamber temperature: 293 K / Instrument: FEI VITROBOT MARK III

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 3.0 µm / Nominal defocus min: 0.6 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Digitization - Dimensions - Width: 3838 pixel / Digitization - Dimensions - Height: 3710 pixel / Number real images: 4845 / Average exposure time: 12.0 sec. / Average electron dose: 4.3 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 3243
Details: 325648 particles were selected from 4845 micrograph movies
CTF correctionSoftware - Name: CTFFIND (ver. 4) / Software - details: grigoriefflab
Startup modelType of model: NONE / Details: the initial model was obtained de novo
Initial angle assignmentType: OTHER / Software - Name: Scipion (ver. 2.0) / Software - details: xmipp3 - ransac
Details: initial non-linear dimensionality reduction approach
Final 3D classificationNumber classes: 3 / Avg.num./class: 50000 / Software - Name: Scipion (ver. 2.0) / Software - details: relion - 3D classification
Final angle assignmentType: OTHER
Software:
Namedetails
Scipion (ver. 2.0)highres
Scipion (ver. 2.0)relion 3.0

Details: highres is based on a projection matching approach with modifications.
Final reconstructionNumber classes used: 1 / Applied symmetry - Point group: C2 (2 fold cyclic) / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 3.7 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.0) / Number images used: 66130
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelPDB ID:

1jb0

RefinementSpace: REAL / Protocol: FLEXIBLE FIT
Output model

PDB-7qco:
The structure of Photosystem I tetramer from Chroococcidiopsis TS-821, a thermophilic, unicellular, non-heterocyst-forming cyanobacterium

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