Type IV conjugative transfer system protein TraV / Type IV conjugative transfer system lipoprotein (TraV) / Type IV conjugative transfer system lipoprotein TraV
Function and homology information
Biological species
Salmonella enterica (bacteria)
Method
single particle reconstruction / cryo EM / Resolution: 5.7 Å
Journal: Nat Commun / Year: 2021 Title: Architecture of the outer-membrane core complex from a conjugative type IV secretion system. Authors: Himani Amin / Aravindan Ilangovan / Tiago R D Costa / Abstract: Conjugation is one of the most important processes that bacteria utilize to spread antibiotic resistance genes among bacterial populations. Interbacterial DNA transfer requires a large double ...Conjugation is one of the most important processes that bacteria utilize to spread antibiotic resistance genes among bacterial populations. Interbacterial DNA transfer requires a large double membrane-spanning nanomachine called the type 4 secretion system (T4SS) made up of the inner-membrane complex (IMC), the outer-membrane core complex (OMCC) and the conjugative pilus. The iconic F plasmid-encoded T4SS has been central in understanding conjugation for several decades, however atomic details of its structure are not known. Here, we report the structure of a complete conjugative OMCC encoded by the pED208 plasmid from E. coli, solved by cryo-electron microscopy at 3.3 Å resolution. This 2.1 MDa complex has a unique arrangement with two radial concentric rings, each having a different symmetry eventually contributing to remarkable differences in protein stoichiometry and flexibility in comparison to other OMCCs. Our structure suggests that F-OMCC is a highly dynamic complex, with implications for pilus extension and retraction during conjugation.
History
Deposition
Jul 18, 2021
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Header (metadata) release
Dec 1, 2021
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Map release
Dec 1, 2021
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Update
Dec 8, 2021
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Current status
Dec 8, 2021
Processing site: PDBe / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
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