|Entry||Database: EMDB / ID: 1269|
|Title||Structure of bacteriophage SPP1 tail reveals trigger for DNA ejection.|
|Sample||Tip of the Tail of the bacteriophage SPP1|
|Source||Bacillus phage SPP1 / virus / Bacteriophage SPP1 / image: Bacillus subtilis|
|Map data||ccp4 map of the tip of the bacteriophage spp1|
|Method||single particle reconstruction, at 20 Å resolution|
|Authors||Plisson C / White HE / Auzat I / Sao-jose C / Lhuillier S / Tavares P / Orlova EV|
|Citation||EMBO J., 2007, 26, 3720-3728|
|Date||Deposition: Sep 21, 2006 / Header (metadata) release: Sep 22, 2006 / Map release: Sep 7, 2007 / Last update: Dec 11, 2013|
Downloads & links
|File||emd_1269.map.gz (map file in CCP4 format, 3251 KB)|
|Projections & slices|
Images are generated by Spider package.
|Voxel size||X=Y=Z: 3.3 Å|
CCP4 map header:
-Entire Tip of the Tail of the bacteriophage SPP1
|Entire||Name: Tip of the Tail of the bacteriophage SPP1|
Details: The total number of proteins that compose the tail Tip is still under investigation.
Number of components: 2
-Component #1: protein, interface tail-tip
|Protein||Name: interface tail-tip / a.k.a: gp19.1 / Recombinant expression: No|
|Mass||Experimental: 28.5 kDa|
|Source||Species: Bacillus phage SPP1 / virus / Bacteriophage SPP1 / image: Bacillus subtilis|
-Component #2: protein, tip fiber
|Sample solution||Buffer solution: 100 mM Tris-Cl, 100 mM NaCl, 10 mM MgCl2 / pH: 7.5|
|Support film||300 mesh copper grid|
|Staining||3.5 microliter of sample was applied onto carbon-coated copper grids, which were glow-discharged for 30 seconds. The sample was left for 1-2 minutes on the grids before blotting and staining with a solution of 2% uranyl acetate.|
|Vitrification||Cryogen name: NONE|
-Electron microscopy imaging
|Imaging||Microscope: FEI TECNAI 12 / Details: Low Dose Imaging|
|Electron gun||Electron source: TUNGSTEN HAIRPIN / Accelerating voltage: 120 kV / Electron dose: 10 e/Å2 / Illumination mode: FLOOD BEAM|
|Lens||Magnification: 42000 X (nominal), 43475 X (calibrated)|
Astigmatism: objective lens astigmatism was corrected at 150,000 times magnification
Cs: 2 mm / Imaging mode: BRIGHT FIELD / Defocus: 600 - 2000 nm
|Specimen Holder||Holder: Gatan single tilt negative stain holder / Model: OTHER / Temperature: 298 K|
|Camera||Detector: KODAK SO-163 FILM|
|Image acquisition||Number of digital images: 20 / Scanner: ZEISS SCAI / Sampling size: 7 microns / Bit depth: 8 / OD range: 2.5|
|Processing||Method: single particle reconstruction / Number of projections: 364 / Applied symmetry: C3 (3 fold cyclic)|
|3D reconstruction||Algorithm: angular reconstitution / Software: IMAGIC|
Details: The 3D reconstruction has been caried out using a C3 symmetry and the exact-filter back projection method.
Resolution: 20 Å / Resolution method: FSC 0.5
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