- EMDB-12119: Cropped map of the wild type Tetrahymena axoneme 96nm-repeat, obt... -
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基本情報
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データベース: EMDB / ID: EMD-12119
タイトル
Cropped map of the wild type Tetrahymena axoneme 96nm-repeat, obtained by cryo-ET and subtomogram averaging, with the structures of the next-dynein regulatory complex (N-DRC) and of the Ccdc96/Ccdc113 complex.
マップデータ
Cropped map of the Tetrahymena axoneme 96nm-repeat, by cryo-ET and subtomogram averaging, with the structures of the next-dynein regulatory complex (N-DRC) and of the Ccdc96/Ccdc113 complex.
試料
細胞器官・細胞要素: Ccdc113/Ccdc96 complex, a novel regulator of ciliary beating that connects radial spoke 3 to dynein g and the nexin link.
H2020 Marie Curie Actions of the European Commission
753954
ドイツ
Polish National Science Centre
2014/14/M/NZ3/00511
ポーランド
Polish National Science Centre
No 2016/23/N/NZ3/02420
ポーランド
引用
ジャーナル: PLoS Genet / 年: 2021 タイトル: Ccdc113/Ccdc96 complex, a novel regulator of ciliary beating that connects radial spoke 3 to dynein g and the nexin link. 著者: Rafał Bazan / Adam Schröfel / Ewa Joachimiak / Martyna Poprzeczko / Gaia Pigino / Dorota Wloga / 要旨: Ciliary beating requires the coordinated activity of numerous axonemal complexes. The protein composition and role of radial spokes (RS), nexin links (N-DRC) and dyneins (ODAs and IDAs) is well ...Ciliary beating requires the coordinated activity of numerous axonemal complexes. The protein composition and role of radial spokes (RS), nexin links (N-DRC) and dyneins (ODAs and IDAs) is well established. However, how information is transmitted from the central apparatus to the RS and across other ciliary structures remains unclear. Here, we identify a complex comprising the evolutionarily conserved proteins Ccdc96 and Ccdc113, positioned parallel to N-DRC and forming a connection between RS3, dynein g, and N-DRC. Although Ccdc96 and Ccdc113 can be transported to cilia independently, their stable docking and function requires the presence of both proteins. Deletion of either CCDC113 or CCDC96 alters cilia beating frequency, amplitude and waveform. We propose that the Ccdc113/Ccdc96 complex transmits signals from RS3 and N-DRC to dynein g and thus regulates its activity and the ciliary beat pattern.
ダウンロード / ファイル: emd_12119.map.gz / 形式: CCP4 / 大きさ: 1.3 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
Cropped map of the Tetrahymena axoneme 96nm-repeat, by cryo-ET and subtomogram averaging, with the structures of the next-dynein regulatory complex (N-DRC) and of the Ccdc96/Ccdc113 complex.
A: 539.0 Å / B: 560.0 Å / C: 385.0 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
7
7
7
M x/y/z
77
80
55
origin x/y/z
0.000
0.000
0.000
length x/y/z
539.000
560.000
385.000
α/β/γ
90.000
90.000
90.000
MAP C/R/S
1
2
3
start NC/NR/NS
3
0
0
NC/NR/NS
77
80
55
D min/max/mean
8.491
225.457
107.732
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添付データ
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試料の構成要素
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全体 : Ccdc113/Ccdc96 complex, a novel regulator of ciliary beating that...
全体
名称: Ccdc113/Ccdc96 complex, a novel regulator of ciliary beating that connects radial spoke 3 to dynein g and the nexin link.
要素
細胞器官・細胞要素: Ccdc113/Ccdc96 complex, a novel regulator of ciliary beating that connects radial spoke 3 to dynein g and the nexin link.
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超分子 #1: Ccdc113/Ccdc96 complex, a novel regulator of ciliary beating that...
超分子
名称: Ccdc113/Ccdc96 complex, a novel regulator of ciliary beating that connects radial spoke 3 to dynein g and the nexin link. タイプ: organelle_or_cellular_component / ID: 1 / 親要素: 0